##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9371342
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.747877945335897	33.0	31.0	34.0	30.0	34.0
2	31.45723686106003	33.0	31.0	34.0	28.0	34.0
3	31.7713613482466	33.0	31.0	34.0	30.0	34.0
4	33.08924847689904	37.0	35.0	37.0	28.0	37.0
5	34.4805679912226	37.0	35.0	37.0	29.0	37.0
6	35.10703621743823	37.0	35.0	37.0	32.0	37.0
7	35.232794299898565	37.0	35.0	37.0	32.0	37.0
8	35.386325459043114	37.0	35.0	37.0	32.0	37.0
9	37.1141248499948	39.0	37.0	39.0	33.0	39.0
10-11	37.00856462180123	39.0	37.0	39.0	33.0	39.0
12-13	36.900298164339745	39.0	37.0	39.0	32.0	39.0
14-15	38.22318943220725	40.0	38.0	41.0	32.0	41.0
16-17	38.22127188400552	40.0	38.0	41.0	32.5	41.0
18-19	38.17550970821468	40.0	38.0	41.0	32.5	41.0
20-21	38.232033497095046	40.0	38.0	41.0	32.0	41.0
22-23	38.27033124913564	40.0	38.0	41.0	32.5	41.0
24-25	38.16204329703463	40.0	38.0	41.0	32.0	41.0
26-27	38.08005386690577	40.0	38.0	41.0	32.0	41.0
28-29	38.084095800608694	40.0	38.0	41.0	32.0	41.0
30-31	38.02825888649498	40.0	37.0	41.0	32.0	41.0
32-33	37.92006345809683	40.0	37.0	41.0	32.0	41.0
34-35	37.80386042311392	40.0	37.0	41.0	31.0	41.0
36-37	37.75689933922395	40.0	37.0	41.0	31.0	41.0
38-39	37.6747006356602	40.0	37.0	41.0	31.0	41.0
40-41	37.608249180258404	40.0	37.0	41.0	31.0	41.0
42-43	37.5892549551094	40.0	37.0	41.0	31.0	41.0
44-45	37.5278769878903	40.0	36.0	41.0	31.0	41.0
46-47	37.40108359463673	40.0	36.0	41.0	30.0	41.0
48-49	37.57638252803817	40.0	37.0	41.0	31.0	41.0
50-51	37.524561639915284	40.0	36.0	41.0	31.0	41.0
52-53	37.467874766422746	40.0	36.0	41.0	31.0	41.0
54-55	37.34673276441984	39.0	36.0	41.0	30.0	41.0
56-57	37.21401797345257	39.0	35.0	41.0	30.0	41.0
58-59	37.10628600654621	39.0	35.0	41.0	30.5	41.0
60-61	36.902176543691	39.0	35.0	41.0	30.5	41.0
62-63	36.73095917178746	38.5	35.0	41.0	30.0	41.0
64-65	36.48262969915726	38.0	35.0	40.0	30.0	41.0
66-67	36.27346887443045	37.5	35.0	40.0	30.0	41.0
68-69	36.021177556752285	37.0	34.0	40.0	30.0	41.0
70-71	35.7833020926834	37.0	34.0	39.0	30.0	41.0
72-73	35.486408819196946	36.0	34.0	39.0	30.0	41.0
74-75	35.31409814774822	36.0	34.0	39.0	30.0	40.5
76-77	35.121684902144466	35.5	34.0	38.5	30.0	40.0
78-79	34.863138740679304	35.0	34.0	37.0	30.0	39.0
80-81	34.60646096085307	35.0	34.0	37.0	30.0	39.0
82-83	34.352146592039944	35.0	34.0	36.5	30.0	39.0
84-85	34.13650199820933	35.0	34.0	36.0	30.0	37.0
86-87	33.9445459956662	35.0	34.0	36.0	29.5	37.0
88-89	33.80004875936096	35.0	34.0	35.0	29.0	37.0
90-91	33.66204341789266	35.0	34.0	35.0	29.0	36.0
92-93	33.61525705269539	35.0	34.0	35.0	29.0	36.0
94-95	33.5859897412758	35.0	34.0	35.0	29.5	36.0
96-97	33.59320708244367	35.0	34.0	35.0	30.0	36.0
98-99	33.625846755146796	35.0	34.0	35.0	30.0	35.5
100-101	33.23012484881981	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	7.0
17	104.0
18	611.0
19	2501.0
20	6887.0
21	14469.0
22	24126.0
23	36138.0
24	52908.0
25	75840.0
26	107570.0
27	143988.0
28	158206.0
29	143045.0
30	171265.0
31	210656.0
32	265360.0
33	346866.0
34	475804.0
35	666914.0
36	1016854.0
37	1756104.0
38	2320607.0
39	1309306.0
40	65205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.51377508151981	21.310288323700064	14.791638166657453	24.384298428122676
2	13.956154941419316	21.026194498456853	42.575137981090016	22.442512579033817
3	17.14447554460949	27.137051416224256	31.195724333799994	24.522748705366258
4	11.697074551380137	18.844963848373588	39.61939011554058	29.83857148470569
5	12.558455045317066	38.21393479498691	36.18800542592579	13.039604733770242
6	28.44333511201931	39.258895556677274	19.1109040426859	13.186865288617517
7	25.110570282012656	33.35891372903039	23.843316056070805	17.68719993288615
8	23.48806147110693	37.44430466775303	21.62441335494969	17.443220506190336
9	24.892509817986173	17.29673970190542	21.808195450780097	36.002555029328306
10-11	23.56822183195129	27.339735837971833	29.36250939322357	19.729532936853303
12-13	24.503849724756734	24.6320374507833	29.3854966441075	21.478616180352464
14-15	21.67361453504059	26.00786152289115	27.056212188236273	25.26231175383199
16-17	21.226317471254223	29.181039176548353	27.651528995313207	21.941114356884214
18-19	21.14357727343278	28.27820007737531	29.48131594172989	21.096906707462022
20-21	22.108094138317075	27.463065944326452	29.269651118343575	21.159188799012895
22-23	21.63949720721981	27.541703807564076	29.199943567365604	21.61885541785051
24-25	21.42362570772402	27.68005044589421	29.475067469778992	21.421256376602773
26-27	21.51342676631001	27.917740476464203	29.297129887166445	21.271702870059336
28-29	21.295948045229785	27.861068443301342	29.429508082433514	21.413475429035365
30-31	21.668776302980355	27.960694116296143	29.123157703991215	21.247371876732284
32-33	21.899816500271935	27.744107093650328	29.09960099072145	21.25647541535629
34-35	21.642536984470578	27.709935017617575	29.217138287175743	21.430389710736108
36-37	21.537478896378584	27.86033200328035	29.247205510214343	21.35498359012672
38-39	21.41573818508077	27.638438988084474	29.407648047467827	21.53817477936693
40-41	21.689646312655622	27.580082879099805	29.278360080709053	21.45191072753552
42-43	21.8098015051608	27.55986447538561	29.08398945134933	21.546344568104264
44-45	21.61438579592052	27.97530192960704	28.815489945997815	21.594822328474628
46-47	21.940880867080768	27.568466187834233	28.377164673888068	22.11348827119694
48-49	21.871391347718703	27.646564734927985	28.477735682694714	22.0043082346586
50-51	21.53838159566458	27.830293107000276	28.495313940304932	22.136011357030213
52-53	21.69992347710679	27.83473530824369	28.59540606966701	21.86993514498251
54-55	21.80408583220659	27.751477294631428	28.39222948391183	22.05220738925016
56-57	22.014660768188072	28.051353793175497	27.999325367694038	21.934660070942392
58-59	21.686658427243998	28.302738004319217	28.464164398962026	21.54643916947476
60-61	21.932470330315795	28.64228554564076	27.819422110184416	21.60582201385903
62-63	21.69415433155264	28.804075466997286	27.799748172991475	21.7020220284586
64-65	21.59882198989027	28.70775509207132	27.912583539194003	21.780839378844412
66-67	21.783744346049588	28.698586562080173	27.725255023967186	21.792414067903053
68-69	21.821363739780093	28.43069993028581	27.951689321941963	21.79624700799213
70-71	21.86819403304314	27.88557702872786	28.109002034126107	22.137226904102896
72-73	21.99660059915697	27.928466094193915	28.146908739975363	21.928024566673752
74-75	21.68528784948274	28.091423457112857	28.100108969625857	22.12317972377855
76-77	21.79040997077245	28.13017851092832	27.997714012625753	22.081697505673475
78-79	21.88453482701742	28.123546042971842	27.983093460957342	22.0088256690534
80-81	21.758856049194037	28.03450701637947	28.08312667842159	22.123510256004902
82-83	21.90787407629114	27.991340317906012	28.17905500113902	21.921730604663832
84-85	22.178168620631702	27.962901293851196	27.953859899102174	21.905070186414925
86-87	21.694535710235318	28.109062045901865	28.107791059968747	22.088611183894066
88-89	21.928427946558067	27.972039390096583	28.069964008779714	22.029568654565644
90-91	21.997554060440812	28.03698135746137	28.118166617872458	21.84729796422536
92-93	21.98638420961891	28.045187438794105	27.990004173755956	21.97842417783103
94-95	21.805481521211714	28.11122846244371	28.036587128949986	22.04670288739459
96-97	21.72482383736248	28.17662437008942	28.18262031437887	21.91593147816923
98-99	21.943111043285622	28.770982253513782	28.115714757665017	21.17019194553558
100-101	22.680639764613346	31.30941746349117	25.31968256963763	20.690260202257853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2149.0
1	1820.0
2	2457.0
3	3523.5
4	4640.5
5	7590.0
6	9945.5
7	11407.0
8	11978.5
9	10956.0
10	9234.5
11	7759.0
12	8544.5
13	12426.0
14	22220.5
15	35925.0
16	47073.0
17	53536.0
18	54854.0
19	52024.0
20	47200.0
21	42607.0
22	41542.5
23	46317.5
24	56401.0
25	71834.5
26	93223.5
27	116009.0
28	136067.5
29	155953.5
30	174577.5
31	192186.5
32	216896.0
33	246105.0
34	274408.5
35	298419.0
36	324440.5
37	346950.0
38	354365.5
39	358687.5
40	369212.5
41	376194.5
42	377340.0
43	382636.0
44	395825.5
45	412652.0
46	413682.5
47	408015.5
48	397604.0
49	379678.5
50	364247.5
51	347627.0
52	328665.0
53	308384.5
54	287674.0
55	265808.0
56	243411.0
57	219674.5
58	193446.5
59	167156.5
60	139486.0
61	109629.0
62	83824.0
63	62522.5
64	45698.5
65	33545.0
66	24673.0
67	18642.5
68	14163.5
69	10496.5
70	7854.5
71	5593.5
72	4080.5
73	3007.5
74	2163.5
75	1553.5
76	1074.0
77	728.0
78	527.0
79	381.0
80	278.5
81	194.5
82	138.0
83	96.5
84	70.0
85	57.5
86	47.5
87	40.0
88	25.5
89	11.5
90	6.5
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.5
99	2.5
100	2.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.6138350302443345
3	0.005815602503888984
4	8.211374635564468
5	4.0549155072987415E-4
6	0.07203877523624685
7	0.023347776657814855
8	4.801873627064299E-4
9	2.2408743592966727E-4
10-11	0.2651007721199376
12-13	0.278119185064423
14-15	2.667707570591277E-5
16-17	9.070205740010341E-5
18-19	0.41517532921112044
20-21	1.0672758962089132E-4
22-23	1.2811024313455873E-4
24-25	0.15649958692375382
26-27	0.13391235758088194
28-29	8.019421113275285E-5
30-31	8.56523208486689E-5
32-33	1.234241420197599E-4
34-35	2.4223197059368472E-4
36-37	2.486747796633333E-4
38-39	0.0013869174416453126
40-41	0.0018129029395865155
42-43	0.0012304216737839848
44-45	9.464308450497296E-4
46-47	0.0015020365252528343
48-49	0.002579850785749245
50-51	2.6956356110899825E-4
52-53	0.0015039423245059825
54-55	4.4921876481291583E-4
56-57	0.007313478045198002
58-59	1.6651678173967456E-4
60-61	0.0028538704762172554
62-63	0.0014691008331926456
64-65	0.0014912696289132018
66-67	0.004980494718377864
68-69	0.0023084467260815985
70-71	0.0026639994381383006
72-73	0.0036362801730663048
74-75	7.558228330429062E-4
76-77	3.820063420956367E-4
78-79	8.327290496196593E-4
80-81	4.2471238207827006E-4
82-83	0.0016305545454607477
84-85	3.4509021175770663E-4
86-87	3.2822056477820424E-4
88-89	9.895649667424885E-5
90-91	2.5775305066845034E-4
92-93	0.0
94-95	0.0
96-97	5.980988232704701E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1052.0
20-21	2569.0
22-23	3429.0
24-25	4275.0
26-27	5703.0
28-29	9314.0
30-31	21279.0
32-33	26974.0
34-35	35766.0
36-37	51861.0
38-39	64300.0
40-41	65059.0
42-43	76277.0
44-45	91925.0
46-47	91147.0
48-49	83449.0
50-51	75143.0
52-53	72458.0
54-55	77486.0
56-57	83482.0
58-59	84191.0
60-61	86465.0
62-63	87409.0
64-65	93823.0
66-67	101789.0
68-69	104159.0
70-71	94660.0
72-73	82104.0
74-75	81365.0
76-77	84677.0
78-79	88567.0
80-81	89273.0
82-83	84477.0
84-85	84397.0
86-87	85262.0
88-89	89125.0
90-91	93050.0
92-93	93285.0
94-95	102227.0
96-97	153384.0
98-99	741815.0
100-101	5822890.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.51137835170067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.786977942476	74.14722907658215
2	7.444736540085464	12.434404198556164
3	2.0664137041285504	5.177071700298559
4	0.7918931383744172	2.6452834997160646
5	0.37240344428298777	1.5549962467496534
6	0.20014332875834445	1.002854715150415
7	0.11637185455259295	0.6802861782521469
8	0.06468382236976662	0.43214681305246244
9	0.0422917923004601	0.31786612811777387
>10	0.11194590623877917	1.3203431141565212
>50	0.0012050273642522395	0.06820425494965392
>100	8.299208142856422E-4	0.11911301411088923
>500	6.214705761035015E-5	0.04046456903407346
>1k	4.143119649955777E-5	0.059736491273554446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.134166056473022E-5	0.0	0.0	0.0
5	0.0	3.201249084709533E-5	0.0	0.0	0.0
6	0.0	3.201249084709533E-5	0.0	1.067083028236511E-5	0.0
7	0.0	3.201249084709533E-5	0.0	1.067083028236511E-5	0.0
8	0.0	3.201249084709533E-5	0.0	1.067083028236511E-5	0.0
9	0.0	5.335415141182554E-5	0.0	2.560999267767626E-4	0.0
10-11	0.0	5.335415141182554E-5	0.0	2.827770024826754E-4	0.0
12-13	0.0	9.070205740010343E-5	0.0	3.0945407818858817E-4	0.0
14-15	0.0	1.1737913310601619E-4	0.0	3.0945407818858817E-4	0.0
16-17	0.0	1.2271454824719876E-4	0.0	3.0945407818858817E-4	0.0
18-19	0.0	1.3338537852956387E-4	0.0	3.2546032361213586E-4	0.0
20-21	0.0	1.4939162395311153E-4	0.0	3.307957387533184E-4	0.0
22-23	0.0	1.653978693766592E-4	0.0	3.5747281445923113E-4	0.0
24-25	0.0	1.8140411480020686E-4	0.0	4.375040415769694E-4	0.0
26-27	0.0	1.974103602237545E-4	0.0	6.082373260948112E-4	0.0
28-29	0.0	2.080811905061196E-4	0.0	0.0011044309342247887	0.0
30-31	0.0	2.454290964943975E-4	0.0	0.003361311538945009	0.0
32-33	0.0	2.454290964943975E-4	0.0	0.00931563483650474	0.0
34-35	0.0	2.8811241762385795E-4	0.0	0.017473484587372863	0.0
36-37	0.0	3.361311538945009E-4	0.0	0.02901932295289191	0.0
38-39	0.0	4.054915507298741E-4	0.0	0.04511093501869849	0.0
40-41	0.0	4.4817487185933453E-4	0.0	0.060444918134457154	0.0
42-43	0.0	4.4817487185933453E-4	0.0	0.07337796443668367	0.0
44-45	0.0	4.8552277784761244E-4	0.0	0.08700995012240509	0.0
46-47	0.0	5.121998535535252E-4	0.0	0.10031647548451439	0.0
48-49	0.0	5.121998535535252E-4	0.0	0.11235850745816342	0.0
50-51	0.0	5.228706838358903E-4	0.0	0.12211164633624512	0.0
52-53	0.0	5.228706838358903E-4	0.0	0.1309577646403258	0.0
54-55	0.0	5.335415141182555E-4	0.0	0.13962781424474743	0.0
56-57	0.0	5.335415141182555E-4	0.0	0.14644647479517875	0.0
58-59	0.0	5.38876929259438E-4	0.0	0.15218738148709118	0.0
60-61	0.0	5.442123444006205E-4	0.0	0.15849384218396895	0.0
62-63	1.067083028236511E-5	5.442123444006205E-4	0.0	0.16425075512130494	0.0
64-65	1.067083028236511E-5	5.86895665530081E-4	0.0	0.16933007033571074	0.0
66-67	1.067083028236511E-5	5.975664958124461E-4	0.0	0.17436136681384587	0.0
68-69	1.067083028236511E-5	6.509206472242717E-4	0.0	0.17941934036768692	0.0
70-71	1.067083028236511E-5	6.669268926478194E-4	0.0	0.1844666430912456	0.0
72-73	1.067083028236511E-5	7.042747986360972E-4	0.0	0.18850021693797964	0.0
74-75	1.067083028236511E-5	7.202810440596448E-4	0.0	0.19182418057093636	0.0
76-77	1.067083028236511E-5	7.522935349067401E-4	0.0	0.19469463391689257	0.0
78-79	1.067083028236511E-5	7.736351954714703E-4	0.0	0.19739968939347213	0.0
80-81	1.067083028236511E-5	7.89641440895018E-4	0.0	0.19858948696995585	0.0
82-83	1.067083028236511E-5	7.949768560362006E-4	0.0	0.19914437014463882	0.0
84-85	1.067083028236511E-5	8.003122711773831E-4	0.0	0.19946449505310979	0.0
86-87	1.067083028236511E-5	8.003122711773831E-4	0.0	0.19959254501649817	0.0
88-89	1.067083028236511E-5	8.37660177165661E-4	0.0	0.19962989292248645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11750	0.0	54.534435	1
GTATCAA	20940	0.0	41.350582	1
CTTATAC	10175	0.0	39.540897	1
ATCAACG	25715	0.0	33.023952	3
TCAACGC	26530	0.0	32.06	4
TATACAC	13765	0.0	31.912725	3
TATCAAC	27305	0.0	31.458567	2
CAACGCA	27175	0.0	29.763216	5
AACGCAG	28480	0.0	28.60979	6
TTATACA	15910	0.0	26.358318	2
ACGCAGA	31875	0.0	25.44975	7
CGCAGAG	32575	0.0	24.935266	8
ACATCTC	16615	0.0	22.769884	8
ACACATC	16975	0.0	22.159811	6
GCAGAGT	36780	0.0	21.89951	9
TACACAT	19590	0.0	21.22722	5
ATACACA	22960	0.0	20.68925	4
CACATCT	18795	0.0	19.863094	7
CATCTCC	19125	0.0	19.714706	9
TACATGG	25305	0.0	19.646578	2
>>END_MODULE