FastQCFastQC Report
Fri 27 May 2016
SRR2139962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16231787
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199400.052.241321
GTATCAA361300.042.1348081
ATCAACG441750.033.6012153
TCAACGC458200.032.4942364
TATCAAC473500.031.77972
CTTATAC274150.031.1192281
CAACGCA468700.030.3712045
AACGCAG491950.029.1724686
TATACAC328350.027.7963313
ACGCAGA554600.025.7581587
CGCAGAG561550.025.4477448
TTATACA365450.024.166742
GCAGAGT645900.021.8484949
GTGGTAT96250.021.7031651
ACATCTC396150.020.2473688
ATACACA485050.019.9924094
TACATGG431950.019.900542
GAGTACT401150.019.61827912-13
TGGTATC99100.019.3535122
TACACAT443800.019.2870625
Produced by FastQC (version 0.11.5)