##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16231787
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.775394415907503	33.0	31.0	34.0	30.0	34.0
2	31.45573497237242	33.0	31.0	34.0	28.0	34.0
3	31.791297532428192	33.0	31.0	34.0	30.0	34.0
4	32.9488836318515	37.0	35.0	37.0	26.0	37.0
5	34.41694669847504	37.0	35.0	37.0	28.0	37.0
6	35.09447567294963	37.0	35.0	37.0	32.0	37.0
7	35.24082739626881	37.0	35.0	37.0	32.0	37.0
8	35.38209415882552	37.0	35.0	37.0	32.0	37.0
9	37.13970525857689	39.0	37.0	39.0	33.0	39.0
10-11	37.01490436635227	39.0	37.0	39.0	33.0	39.0
12-13	36.90326228406028	39.0	37.0	39.0	32.0	39.0
14-15	38.241269430161935	40.0	38.0	41.0	32.0	41.0
16-17	38.231934074787944	40.0	38.0	41.0	32.0	41.0
18-19	38.17139280474787	40.0	38.0	41.0	32.0	41.0
20-21	38.24142206762022	40.0	38.0	41.0	32.0	41.0
22-23	38.27844263784775	40.0	38.0	41.0	32.0	41.0
24-25	38.157180903210325	40.0	38.0	41.0	32.0	41.0
26-27	38.06043521428174	40.0	38.0	41.0	32.0	41.0
28-29	38.05654708736999	40.0	38.0	41.0	32.0	41.0
30-31	38.009486997605464	40.0	37.0	41.0	32.0	41.0
32-33	37.899260998506506	40.0	37.0	41.0	31.5	41.0
34-35	37.783783216525876	40.0	37.0	41.0	31.0	41.0
36-37	37.74066434253551	40.0	37.0	41.0	31.0	41.0
38-39	37.657169731724025	40.0	37.0	41.0	31.0	41.0
40-41	37.58665619794208	40.0	37.0	41.0	30.0	41.0
42-43	37.570219943775676	40.0	37.0	41.0	30.0	41.0
44-45	37.50340916857213	40.0	36.0	41.0	30.0	41.0
46-47	37.37357030216767	40.0	36.0	41.0	30.0	41.0
48-49	37.555935571410586	40.0	36.5	41.0	30.0	41.0
50-51	37.49128300338411	40.0	36.0	41.0	30.0	41.0
52-53	37.44182425588832	40.0	36.0	41.0	30.0	41.0
54-55	37.32189821364261	39.0	36.0	41.0	30.0	41.0
56-57	37.171819945962525	39.0	35.0	41.0	30.0	41.0
58-59	37.069617994422956	39.0	35.0	41.0	30.5	41.0
60-61	36.866093872860006	39.0	35.0	41.0	30.0	41.0
62-63	36.699507641572495	38.5	35.0	41.0	30.0	41.0
64-65	36.45486621191067	38.0	35.0	40.0	30.0	41.0
66-67	36.24365062025858	37.5	35.0	40.0	30.0	41.0
68-69	35.99689895170782	37.0	34.0	40.0	30.0	41.0
70-71	35.760039632138216	37.0	34.0	39.5	30.0	41.0
72-73	35.46145623398601	36.0	34.0	39.0	30.0	41.0
74-75	35.28671119943606	36.0	34.0	39.0	30.0	40.5
76-77	35.08803027460395	35.5	34.0	38.5	30.0	40.0
78-79	34.823375543198566	35.0	34.0	37.0	30.0	39.0
80-81	34.568515662793416	35.0	34.0	37.0	30.0	39.0
82-83	34.31131634489703	35.0	34.0	36.5	30.0	39.0
84-85	34.093739857054835	35.0	34.0	36.0	29.0	37.0
86-87	33.90432423911906	35.0	34.0	36.0	29.0	37.0
88-89	33.76661964432541	35.0	34.0	35.0	29.0	37.0
90-91	33.63116505122604	35.0	34.0	35.0	29.0	36.0
92-93	33.59004144465473	35.0	34.0	35.0	29.0	36.0
94-95	33.56509289978392	35.0	34.0	35.0	29.0	36.0
96-97	33.57428639546782	35.0	34.0	35.0	29.5	36.0
98-99	33.610628098165236	35.0	34.0	35.0	30.0	35.5
100-101	33.22540206569768	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	11.0
17	137.0
18	845.0
19	3032.0
20	8615.0
21	18606.0
22	33380.0
23	54248.0
24	85160.0
25	130646.0
26	195324.0
27	276483.0
28	308966.0
29	258110.0
30	302867.0
31	370215.0
32	459565.0
33	597404.0
34	813496.0
35	1137578.0
36	1722341.0
37	2989243.0
38	4047035.0
39	2299496.0
40	118981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.53740891252454	21.041158314854673	14.833240480545982	24.588192292074805
2	13.802985058149778	20.91507769434689	42.56020230657141	22.721734940931924
3	17.121289816890375	26.912162941879092	31.297531250675796	24.66901599055473
4	11.508817006537841	18.84117569441465	39.4463260966178	30.203681202429706
5	12.57546866364333	38.03100062550298	36.095040228904644	13.298490481949035
6	28.428358961772172	39.13009488413364	19.095448078281706	13.346098075812485
7	25.093998335313593	33.33937855938874	23.590296111933675	17.976326993363994
8	23.68630081513936	37.07814269396132	21.681612828918816	17.553943661980508
9	24.796904090380945	17.055312615899464	21.606999619264478	36.54078367445511
10-11	23.492561990773826	27.176985977644897	29.276670313762835	20.053781717818445
12-13	24.51265248396753	24.559381061725077	29.143670229115575	21.78429622519182
14-15	21.72384787627502	26.03274829871497	26.90178144108643	25.34162238392358
16-17	21.289941130243147	29.208824746343037	27.498865804111	22.00236831930282
18-19	21.154412234916972	28.100449120507164	29.344310307609383	21.400828336966484
20-21	22.166499303816963	27.387154235305	29.089794209933462	21.356552250944578
22-23	21.731784780745684	27.66416118864602	28.956759679688915	21.64729435091938
24-25	21.596274317157075	27.7178586742549	29.218792148593735	21.46707485999429
26-27	21.661158271866814	27.9836649413259	28.95981767699724	21.395359109810038
28-29	21.244189501777054	27.81002317347614	29.178574281231505	21.767213043515294
30-31	22.074856390035034	27.658366214704838	29.074930341638172	21.19184705362196
32-33	21.926006845678938	27.809996074040487	29.064701634201555	21.19929544607902
34-35	21.653839434451587	27.88708419151402	29.308374641119144	21.15070173291525
36-37	21.804983665562418	27.514759771780543	29.16035846191817	21.519898100738864
38-39	21.45794572498738	27.44489289722725	29.401903780086414	21.69525759769896
40-41	21.626559943427804	27.35797621460636	29.3971620137316	21.618301828234237
42-43	21.746346651554838	27.286842249385202	29.399490987748976	21.567320111310988
44-45	21.550945073544604	27.7838796292744	28.964590301721405	21.700584995459597
46-47	21.968083136014315	27.304532530213184	28.391972855251602	22.335411478520907
48-49	21.86764799931224	27.410214510673896	28.64966222733382	22.07247526268004
50-51	21.571443663491216	27.74141944793424	28.55834358778764	22.12879330078691
52-53	21.59406001632887	27.956195781239774	28.622977566453706	21.82676663597765
54-55	21.680848249179984	27.88520769435324	28.341172953016326	22.092771103450445
56-57	21.98081119130235	28.159134690208155	27.928659530792892	21.931394587696598
58-59	21.5240220217782	28.478018046813325	28.455616689097145	21.542343242311333
60-61	21.818387573238994	28.752157808898325	27.78302628224669	21.64642833561599
62-63	21.583070509497404	28.9249640635308	27.732086367524587	21.759879059447204
64-65	21.51058111631458	28.823201746201804	27.808467955767576	21.85774918171604
66-67	21.724106477571166	28.73103361577689	27.66966886569947	21.87519104095247
68-69	21.79311692714845	28.451629678809898	27.85560657876723	21.899646815274423
70-71	21.79634119283303	27.875658021485094	28.054583639886754	22.273417145795122
72-73	21.88053892919917	27.880564321500895	28.126156423300124	22.112740325999813
74-75	21.734931666924183	28.051239982464445	28.036011693845865	22.17781665676551
76-77	21.84572679179447	28.06359551070483	27.861695487614075	22.22898220988662
78-79	21.9296637204252	28.06659146654642	27.853818876893683	22.149925936134697
80-81	21.800157726443196	27.955430412581457	27.961624043550298	22.282787817425053
82-83	21.953447664152776	27.919667501639157	28.092870783328877	22.034014050879193
84-85	22.234259682692365	27.957081182583266	27.842093163373292	21.966565971351077
86-87	21.745429781628108	28.09069091407612	27.99597480889063	22.167904495405143
88-89	22.010723301171865	27.928641233233854	27.954272265973884	22.1063631996204
90-91	22.061674185595535	27.998839554892957	28.002348864784043	21.93713739472747
92-93	22.05861353055357	28.040503819974205	27.95387876333255	21.947003886139672
94-95	21.917966071821535	28.071283611760318	28.00285927157089	22.007891044847256
96-97	21.735847446409785	28.190890534543655	28.11111043380729	21.96215158523927
98-99	22.007995343191137	28.710876522670688	28.14709179923005	21.134036334908124
100-101	22.71588133574393	31.231115352766416	25.299684593260242	20.753318718229416
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4171.0
1	3591.0
2	4662.0
3	6514.0
4	8673.0
5	14272.0
6	18696.5
7	21197.5
8	21871.0
9	19792.0
10	16557.5
11	13790.5
12	15452.5
13	22760.5
14	40444.5
15	64933.5
16	84732.5
17	95710.0
18	96779.0
19	90121.0
20	80216.5
21	71030.5
22	67834.5
23	75302.5
24	91971.0
25	116374.5
26	150171.0
27	188060.5
28	223715.5
29	259055.5
30	291499.5
31	323763.5
32	362791.5
33	405156.0
34	452268.0
35	497772.5
36	542339.5
37	580914.5
38	598959.5
39	608466.0
40	628314.0
41	644502.5
42	652943.5
43	668478.0
44	684670.0
45	705577.5
46	736480.5
47	756182.0
48	750956.5
49	720646.0
50	673677.5
51	631437.5
52	592533.5
53	552991.0
54	512197.5
55	468034.5
56	422430.5
57	374638.5
58	323742.5
59	277265.0
60	230779.0
61	179655.0
62	136143.5
63	101235.0
64	72916.5
65	52265.0
66	38061.0
67	29230.5
68	22695.0
69	17150.0
70	12865.0
71	9147.0
72	6564.0
73	4550.0
74	3180.0
75	2295.0
76	1636.0
77	1103.0
78	794.0
79	583.5
80	414.0
81	299.5
82	227.5
83	169.0
84	127.0
85	99.0
86	74.5
87	55.5
88	38.5
89	25.5
90	17.5
91	11.0
92	7.5
93	5.0
94	3.5
95	2.0
96	1.0
97	2.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.7819972625318456
3	0.006271644643932304
4	8.673937133354448
5	3.0803755618528017E-4
6	0.07460669610807485
7	0.02529604411393521
8	5.175030943912706E-4
9	2.895553028141633E-4
10-11	0.2993662989786645
12-13	0.31487907030815526
14-15	7.700938904632004E-5
16-17	1.4169727584522888E-4
18-19	0.48186930989175747
20-21	2.618728288239294E-4
22-23	2.0030882073143288E-4
24-25	0.1743311487220417
26-27	0.1520666791291598
28-29	1.018491201578575E-4
30-31	1.88509790549553E-4
32-33	9.602700651140545E-5
34-35	3.57298914656389E-4
36-37	2.870252870837841E-4
38-39	0.0012428001542829686
40-41	0.001639591244532208
42-43	0.0010533108296073392
44-45	8.026106033528897E-4
46-47	0.0012747536149401383
48-49	0.0022769715387403445
50-51	3.670262796768026E-4
52-53	0.0015036981304997925
54-55	5.713649242568408E-4
56-57	0.009417128892721302
58-59	1.3703212735354818E-4
60-61	0.002537056183698395
62-63	0.0013492640885863017
64-65	0.00135055440258226
66-67	0.004716682961478161
68-69	0.0019900511992514205
70-71	0.002379567769407561
72-73	0.0035958687165134726
74-75	7.096921027237039E-4
76-77	3.3210269562032125E-4
78-79	8.188693770084269E-4
80-81	5.786944941559089E-4
82-83	0.001627057450765182
84-85	2.644316794280327E-4
86-87	4.4615986151197895E-4
88-89	1.4787112407192384E-4
90-91	2.8307520413156585E-4
92-93	0.0
94-95	0.0
96-97	4.354094089360814E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1580.0
20-21	4040.0
22-23	5374.0
24-25	7299.0
26-27	9729.0
28-29	15888.0
30-31	36102.0
32-33	45293.0
34-35	59777.0
36-37	87626.0
38-39	105339.0
40-41	111180.0
42-43	130319.0
44-45	156872.0
46-47	156499.0
48-49	144641.0
50-51	129053.0
52-53	117305.0
54-55	128509.0
56-57	144474.0
58-59	166377.0
60-61	162766.0
62-63	158150.0
64-65	172768.0
66-67	183479.0
68-69	191967.0
70-71	172254.0
72-73	146262.0
74-75	145146.0
76-77	151418.0
78-79	157019.0
80-81	158965.0
82-83	151456.0
84-85	151267.0
86-87	153295.0
88-89	160360.0
90-91	165583.0
92-93	166538.0
94-95	182509.0
96-97	271548.0
98-99	1279280.0
100-101	9986481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.30199965229178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33901682408468	70.93799698291447
2	7.09985341847637	11.402648534836244
3	2.1686563634715768	5.224423276363049
4	0.9650217664013303	3.0997271020005455
5	0.48718474688880764	1.9560954687633445
6	0.2972493763772333	1.4321831591093122
7	0.1779622044818305	1.0003504617694723
8	0.11645609483984694	0.7481325829869317
9	0.08674205069897552	0.6269004112561387
>10	0.25739537869978785	3.03557285443518
>50	0.003153553271750644	0.16496027433623148
>100	0.0010957522743179312	0.16519913723275378
>500	1.062953618229769E-4	0.04771266253894119
>1k	1.0617467164320446E-4	0.1580970914573759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2321502247411206E-5
2	6.160751123705603E-6	0.0	0.0	0.0	1.2321502247411206E-5
3	6.160751123705603E-6	0.0	0.0	0.0	1.2321502247411206E-5
4	6.160751123705603E-6	0.0	0.0	0.0	1.2321502247411206E-5
5	6.160751123705603E-6	6.160751123705603E-6	0.0	0.0	1.2321502247411206E-5
6	6.160751123705603E-6	6.160751123705603E-6	0.0	0.0	1.848225337111681E-5
7	6.160751123705603E-6	6.160751123705603E-6	0.0	6.160751123705603E-6	2.4643004494822413E-5
8	2.4643004494822413E-5	6.160751123705603E-6	0.0	6.160751123705603E-6	3.080375561852802E-5
9	2.4643004494822413E-5	6.160751123705603E-6	0.0	6.160751123705603E-6	4.312525786593922E-5
10-11	8.008976460817284E-5	6.160751123705603E-6	0.0	2.156262893296961E-5	4.6205633427792025E-5
12-13	1.2321502247411207E-4	6.160751123705603E-6	0.0	3.080375561852802E-5	4.9286008989644826E-5
14-15	2.895553028141633E-4	6.160751123705603E-6	0.0	3.388413118038082E-5	8.625051573187844E-5
16-17	5.236638455149762E-4	6.160751123705603E-6	0.0	4.312525786593922E-5	1.1089352022670085E-4
18-19	6.253162390561188E-4	6.160751123705603E-6	0.0	4.9286008989644826E-5	1.1089352022670085E-4
20-21	6.68441496922058E-4	6.160751123705603E-6	0.0	5.5446760113350426E-5	1.1089352022670085E-4
22-23	7.700938904632004E-4	6.160751123705603E-6	0.0	9.857201797928964E-5	1.2937577359781765E-4
24-25	8.409425283858148E-4	6.160751123705603E-6	0.0	1.2629539803596487E-4	1.6017952921634567E-4
26-27	9.30273419679546E-4	6.160751123705603E-6	0.0	3.172786828708386E-4	1.6017952921634567E-4
28-29	0.0011089352022670086	6.160751123705603E-6	0.0	0.0013646063739007912	1.6017952921634567E-4
30-31	0.001133578206761831	6.160751123705603E-6	0.0	0.006049857603478902	1.6325990477819848E-4
32-33	0.001133578206761831	6.160751123705603E-6	0.0	0.017823053000880308	1.848225337111681E-4
34-35	0.001133578206761831	6.160751123705603E-6	0.0	0.034244535121117595	2.1562628932969612E-4
36-37	0.0011366585823236837	6.160751123705603E-6	0.0	0.05786485492940488	2.341085427008129E-4
38-39	0.0011397389578855365	6.160751123705603E-6	0.0	0.09585512673373547	2.5567117163378256E-4
40-41	0.0011397389578855365	6.160751123705603E-6	0.0	0.1554326704755305	2.7723380056675215E-4
42-43	0.0011397389578855365	6.160751123705603E-6	0.0	0.18759179134127377	2.8031417612860496E-4
44-45	0.0011397389578855365	6.160751123705603E-6	0.0	0.22752578012513347	2.895553028141633E-4
46-47	0.0011428193334473893	6.160751123705603E-6	0.0	0.2675953054337147	2.9571605393786894E-4
48-49	0.001145899709009242	6.160751123705603E-6	0.0	0.3000624638556433	3.0803755618528017E-4
50-51	0.001145899709009242	6.160751123705603E-6	0.0	0.328589821933962	3.0803755618528017E-4
52-53	0.001145899709009242	6.160751123705603E-6	0.0	0.36575763346327794	3.3268056068010257E-4
54-55	0.001145899709009242	6.160751123705603E-6	0.0	0.4395357085452144	3.57323565174925E-4
56-57	0.001145899709009242	6.160751123705603E-6	0.0	0.5143457094403715	3.634843162986306E-4
58-59	0.0011520604601329477	6.160751123705603E-6	0.0	0.5609671935690137	3.696450674223362E-4
60-61	0.0011520604601329477	6.160751123705603E-6	0.0	0.5839098307536934	3.819665696697474E-4
62-63	0.0011520604601329477	6.160751123705603E-6	0.0	0.5984584445323242	4.004488230408642E-4
64-65	0.0011520604601329477	6.160751123705603E-6	0.0	0.6092582412521801	4.004488230408642E-4
66-67	0.0011520604601329477	6.160751123705603E-6	0.0	0.6164447574379827	4.004488230408642E-4
68-69	0.0011520604601329477	6.160751123705603E-6	0.0	0.6232431463029917	4.03529198602717E-4
70-71	0.0011520604601329477	6.160751123705603E-6	0.0	0.6293422899154604	4.127703252882754E-4
72-73	0.0011520604601329477	6.160751123705603E-6	0.0	0.6338365578602035	4.49734832030509E-4
74-75	0.0011520604601329477	6.160751123705603E-6	0.0	0.6375083655299321	4.49734832030509E-4
76-77	0.0011520604601329477	6.160751123705603E-6	0.0	0.640752000996563	4.49734832030509E-4
78-79	0.0011520604601329477	6.160751123705603E-6	0.0	0.6435243390022306	4.49734832030509E-4
80-81	0.0011520604601329477	6.160751123705603E-6	0.0	0.644750328475848	4.712974609634786E-4
82-83	0.0011520604601329477	6.160751123705603E-6	0.0	0.6452678315702394	4.990208410201539E-4
84-85	0.0011520604601329477	6.160751123705603E-6	0.0	0.6456097532576051	4.990208410201539E-4
86-87	0.0011520604601329477	6.160751123705603E-6	0.0	0.6457298879045172	4.990208410201539E-4
88-89	0.0011520604601329477	6.160751123705603E-6	0.0	0.6457576112845739	5.082619677057123E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19940	0.0	52.24132	1
GTATCAA	36130	0.0	42.134808	1
ATCAACG	44175	0.0	33.601215	3
TCAACGC	45820	0.0	32.494236	4
TATCAAC	47350	0.0	31.7797	2
CTTATAC	27415	0.0	31.119228	1
CAACGCA	46870	0.0	30.371204	5
AACGCAG	49195	0.0	29.172468	6
TATACAC	32835	0.0	27.796331	3
ACGCAGA	55460	0.0	25.758158	7
CGCAGAG	56155	0.0	25.447744	8
TTATACA	36545	0.0	24.16674	2
GCAGAGT	64590	0.0	21.848494	9
GTGGTAT	9625	0.0	21.703165	1
ACATCTC	39615	0.0	20.247368	8
ATACACA	48505	0.0	19.992409	4
TACATGG	43195	0.0	19.90054	2
GAGTACT	40115	0.0	19.618279	12-13
TGGTATC	9910	0.0	19.353512	2
TACACAT	44380	0.0	19.287062	5
>>END_MODULE