FastQCFastQC Report
Fri 27 May 2016
SRR2139961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18861843
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284150.051.9386671
GTATCAA512850.041.0544621
CTTATAC271450.034.861821
ATCAACG622700.033.1735083
TCAACGC643450.032.166094
TATCAAC653200.031.9267082
CAACGCA650950.030.2774335
TATACAC344150.029.8702983
AACGCAG680200.029.1412896
TTATACA371000.026.3609852
ACGCAGA758300.026.034227
CGCAGAG761050.025.9503828
GCAGAGT857750.022.7509489
ACACATC411900.021.5656266
TACACAT453600.020.7349135
TACATGG557550.020.6397862
GAGTACT543150.020.51026312-13
GTACATG595450.019.9126631
AGAGTAC806950.019.4824110-11
CAGAGTA856100.019.27997410-11
Produced by FastQC (version 0.11.5)