##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18861843
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.805029550929884	33.0	31.0	34.0	30.0	34.0
2	31.470451270323903	33.0	31.0	34.0	29.0	34.0
3	31.79979300007958	33.0	31.0	34.0	30.0	34.0
4	32.91255096333906	37.0	35.0	37.0	26.0	37.0
5	34.40490566059743	37.0	35.0	37.0	28.0	37.0
6	35.14917455309113	37.0	35.0	37.0	32.0	37.0
7	35.27651778248817	37.0	35.0	37.0	32.0	37.0
8	35.413213173283225	37.0	35.0	37.0	32.0	37.0
9	37.15025954780771	39.0	37.0	39.0	33.0	39.0
10-11	37.02837137919131	39.0	37.0	39.0	33.0	39.0
12-13	36.919367025799126	39.0	37.0	39.0	32.0	39.0
14-15	38.25556283126734	40.0	38.0	41.0	32.0	41.0
16-17	38.250082931980714	40.0	38.0	41.0	32.0	41.0
18-19	38.189652119360765	40.0	38.0	41.0	32.0	41.0
20-21	38.2645328551951	40.0	38.0	41.0	32.0	41.0
22-23	38.30838855452566	40.0	38.0	41.0	32.0	41.0
24-25	38.184328579860406	40.0	38.0	41.0	32.0	41.0
26-27	38.08531501894936	40.0	38.0	41.0	32.0	41.0
28-29	38.09298304015134	40.0	38.0	41.0	32.0	41.0
30-31	38.03878033615444	40.0	37.0	41.0	32.0	41.0
32-33	37.92827366313335	40.0	37.0	41.0	32.0	41.0
34-35	37.81577266393441	40.0	37.0	41.0	31.0	41.0
36-37	37.77109357833931	40.0	37.0	41.0	31.0	41.0
38-39	37.691235071108764	40.0	37.0	41.0	31.0	41.0
40-41	37.61284234542731	40.0	37.0	41.0	31.0	41.0
42-43	37.59015709169982	40.0	36.5	41.0	31.0	41.0
44-45	37.52237087037936	40.0	36.0	41.0	30.5	41.0
46-47	37.39003079585752	40.0	36.0	41.0	30.0	41.0
48-49	37.56338400108538	40.0	36.5	41.0	31.0	41.0
50-51	37.51685840421839	40.0	36.0	41.0	30.0	41.0
52-53	37.46703645190908	40.0	36.0	41.0	30.0	41.0
54-55	37.338479377613346	39.0	36.0	41.0	30.0	41.0
56-57	37.20030120770063	39.0	35.0	41.0	30.0	41.0
58-59	37.0931433781236	39.0	35.0	41.0	30.5	41.0
60-61	36.8747260401193	39.0	35.0	41.0	30.0	41.0
62-63	36.70207788831923	38.5	35.0	41.0	30.0	41.0
64-65	36.44954361108992	38.0	35.0	40.0	30.0	41.0
66-67	36.23351133675517	37.5	35.0	40.0	30.0	41.0
68-69	35.98345685362736	37.0	34.0	40.0	30.0	41.0
70-71	35.74652906458931	36.5	34.0	39.0	30.0	41.0
72-73	35.44938166384153	36.0	34.0	39.0	30.0	41.0
74-75	35.275330041823565	36.0	34.0	39.0	30.0	40.5
76-77	35.08215347766858	35.5	34.0	38.5	30.0	40.0
78-79	34.82366631646678	35.0	34.0	37.0	30.0	39.0
80-81	34.57291433378012	35.0	34.0	37.0	30.0	39.0
82-83	34.31841671484551	35.0	34.0	36.0	30.0	39.0
84-85	34.1048083553923	35.0	34.0	36.0	29.5	37.0
86-87	33.91531508002194	35.0	34.0	36.0	29.0	37.0
88-89	33.77688444090473	35.0	34.0	35.0	29.0	37.0
90-91	33.64197891924701	35.0	34.0	35.0	29.0	36.0
92-93	33.59994306534717	35.0	34.0	35.0	29.0	36.0
94-95	33.57453780347863	35.0	34.0	35.0	29.0	36.0
96-97	33.58419238766023	35.0	34.0	35.0	29.5	36.0
98-99	33.61743849217328	35.0	34.0	35.0	30.0	35.5
100-101	33.229385196951895	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	11.0
17	160.0
18	965.0
19	3454.0
20	9290.0
21	19984.0
22	35903.0
23	59365.0
24	93612.0
25	145820.0
26	222468.0
27	319348.0
28	362680.0
29	296997.0
30	346438.0
31	427394.0
32	532426.0
33	698727.0
34	953129.0
35	1342485.0
36	2038270.0
37	3530102.0
38	4713295.0
39	2583250.0
40	126267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.684006488655434	21.050843228840364	14.981738528944389	24.283411753559818
2	13.86389919073017	20.92622496744376	42.642256766227874	22.567619075598202
3	17.057423099734166	27.139150094816717	31.3049724086161	24.498454396833015
4	11.532959759740907	19.03294086596289	39.40325310163167	30.03084627266454
5	12.521471988015556	38.1308576113479	35.994214545135286	13.353455855501261
6	28.20652727550553	39.22781477614221	19.102833876636275	13.462824071715989
7	25.09132475786026	33.453238287564005	23.538750843193572	17.91668611138216
8	23.836771970512153	37.02969334317901	21.454817308438628	17.6787173778702
9	24.931691291132015	17.19120929994104	21.568432742649073	36.30866666627787
10-11	23.5386999061634	27.302230612768604	29.11349952922164	20.045569951846357
12-13	24.667518589201816	24.666720809327472	29.09251635142927	21.573244250041444
14-15	21.838118960486646	25.95439593176185	27.010481381813197	25.197003725938306
16-17	21.259093370533	29.20367485255133	27.519123554261842	22.01810822265383
18-19	21.077074359794096	27.96680750432314	29.63219798265171	21.323920153231057
20-21	22.024324438113922	27.166606376810858	29.476288482240793	21.332780702834427
22-23	21.5858511892847	27.28370592923346	29.449590319009218	21.680852562472623
24-25	21.333002942292733	27.443172249363702	29.764041745460755	21.459783062882806
26-27	21.326746277480144	27.55215425327781	29.631119537266965	21.489979931975075
28-29	20.880032298077257	27.3737785297469	29.801826328776365	21.944362843399478
30-31	21.2484141489905	27.14404317099882	29.88673096897671	21.720811711033967
32-33	21.13243726487373	27.46341742729802	29.769356267104243	21.634789040724005
34-35	21.168478807184712	27.78328806992224	29.873268508771293	21.174964614121755
36-37	21.43489454780944	27.432709986990115	29.42948746990968	21.70290799529076
38-39	21.32625514762258	27.330661488798352	29.723338429586743	21.619744933992326
40-41	21.555257375408786	27.230466180737693	29.68468291540215	21.529593528451368
42-43	21.707851947233152	27.222332675029737	29.56223649756372	21.50757888017339
44-45	21.499317863817712	27.719836446238105	29.13911618867655	21.641729501267637
46-47	21.925188928313478	27.357237323439815	28.493364517597204	22.224209230649507
48-49	21.836009897029363	27.38911599783042	28.65714298017962	22.117731124960592
50-51	21.457476106205444	27.706414651178722	28.642366330636616	22.193742911979218
52-53	21.559654742239847	27.862975196790764	28.734327917711646	21.843042143257748
54-55	21.700211261967837	27.783890334805346	28.410394425655603	22.10550397757121
56-57	21.942186713534365	28.094834382833938	27.98385688832763	21.97912201530407
58-59	21.488889635002252	28.382491899419797	28.538440028001165	21.590178437576785
60-61	21.80113723700419	28.703144639949635	27.895309021176086	21.600409101870085
62-63	21.534929963057206	28.904759333719767	27.83165414176783	21.7286565614552
64-65	21.53011210482747	28.8353303380332	27.844351666541208	21.790205890598124
66-67	21.697669871849662	28.723808046343148	27.71437422222402	21.86414785958317
68-69	21.73780870733301	28.372695867097587	28.004285245394232	21.885210180175175
70-71	21.770445449027534	27.90340687956279	28.145218070491314	22.18092960091836
72-73	21.893649297309498	27.902622264309006	28.2048604095514	21.998868028830092
74-75	21.709679800268276	28.062207574658714	28.113626298865917	22.114486326207086
76-77	21.763845854635605	28.048803930958805	28.037869617010173	22.149480597395417
78-79	21.812636306921245	27.99702221802816	28.014951820745914	22.175389654304677
80-81	21.710892411350088	28.018548974276886	28.085812548281496	22.18474606609153
82-83	21.845636156775377	27.947268112887723	28.194058406388212	22.01303732394869
84-85	22.088109091254076	27.876690235430125	28.02975166736465	22.005449005951146
86-87	21.6287602780717	28.053779056421664	28.170526192594814	22.146934472911827
88-89	21.91451089650924	27.874958388379422	28.14088462753242	22.06964608757892
90-91	21.93552368005864	27.940826092544768	28.181044082328622	21.942606145067966
92-93	21.891260269225317	27.994376581115798	28.08077292484897	22.033590224809917
94-95	21.696473934313413	28.078602024199355	28.116633937666187	22.108290103821037
96-97	21.646322065588027	28.163315244855564	28.197650735730225	21.992711953826188
98-99	21.90921653806027	28.683956612087012	28.25041928483771	21.156407565015005
100-101	22.63456347454075	31.219034568400055	25.435730316745353	20.710671640313844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6899.0
1	5792.5
2	6645.0
3	8808.0
4	11356.0
5	18303.0
6	24108.5
7	28296.0
8	30805.5
9	29357.0
10	24893.5
11	20354.0
12	21641.0
13	30309.0
14	53348.0
15	85970.0
16	112627.0
17	127588.5
18	129609.0
19	121461.0
20	107977.5
21	94309.5
22	87373.5
23	92771.0
24	109224.0
25	135492.0
26	174930.5
27	219102.5
28	258844.5
29	296302.5
30	332190.0
31	370671.5
32	419792.5
33	473013.5
34	526376.0
35	575921.0
36	625079.5
37	670623.0
38	690539.0
39	697200.0
40	718100.0
41	735076.5
42	742468.0
43	763958.5
44	801791.5
45	843552.5
46	844317.0
47	834920.5
48	817143.5
49	773546.0
50	739156.5
51	707098.0
52	670727.0
53	630790.0
54	587730.0
55	540292.5
56	492517.5
57	437456.0
58	375866.5
59	320442.5
60	266874.0
61	207858.5
62	158040.5
63	120294.0
64	88244.0
65	63923.5
66	46509.0
67	34577.5
68	26250.0
69	19693.0
70	14742.5
71	10773.0
72	7749.0
73	5374.5
74	3839.5
75	2775.0
76	1906.5
77	1237.0
78	847.0
79	600.5
80	431.0
81	317.0
82	239.5
83	158.0
84	104.0
85	80.5
86	71.5
87	60.5
88	39.5
89	24.5
90	18.0
91	12.5
92	9.5
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.8272710678378565
3	0.0066006275208631525
4	8.80302630024012
5	3.128008222738361E-4
6	0.07508810247227696
7	0.026312381032966927
8	5.672828471745842E-4
9	2.4387860719655023E-4
10-11	0.30403444668689056
12-13	0.3163211569516298
14-15	9.01290504856816E-5
16-17	1.3254272130247294E-4
18-19	0.5072330418612858
20-21	2.147523233616406E-4
22-23	1.511618258152734E-4
24-25	0.17507234746968964
26-27	0.16563077208098426
28-29	1.4077267144537649E-4
30-31	1.3563924662558145E-4
32-33	9.330002149365923E-5
34-35	3.2616070301426495E-4
36-37	3.1671093015047796E-4
38-39	0.0014708818957136101
40-41	0.0016922423887595775
42-43	0.0010586215079338485
44-45	8.801323719087351E-4
46-47	0.001321144944344818
48-49	0.002403582091602202
50-51	3.3790395281747723E-4
52-53	0.0016084386130817347
54-55	5.248645344823598E-4
56-57	0.010526906875753913
58-59	1.4674410920720088E-4
60-61	0.0024525818361611274
62-63	0.0014082035828534255
64-65	0.0014504117397961338
66-67	0.004647063492396568
68-69	0.0018766828882379196
70-71	0.0023316705882412093
72-73	0.003285296515644382
74-75	6.321802803587972E-4
76-77	3.3713750869709415E-4
78-79	6.946362157372325E-4
80-81	4.009290553385808E-4
82-83	0.0016789769299856566
84-85	2.4395440329566133E-4
86-87	4.830750874442995E-4
88-89	9.010211619374839E-5
90-91	2.455529139518746E-4
92-93	0.0
94-95	0.0
96-97	5.119239895260352E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1837.0
20-21	4580.0
22-23	6437.0
24-25	8431.0
26-27	11811.0
28-29	19260.0
30-31	41007.0
32-33	50813.0
34-35	66822.0
36-37	94789.0
38-39	118508.0
40-41	124902.0
42-43	146892.0
44-45	179911.0
46-47	178896.0
48-49	161950.0
50-51	142365.0
52-53	130592.0
54-55	140223.0
56-57	154047.0
58-59	171668.0
60-61	175957.0
62-63	172270.0
64-65	185169.0
66-67	201002.0
68-69	210474.0
70-71	184918.0
72-73	157132.0
74-75	155351.0
76-77	161442.0
78-79	169488.0
80-81	171819.0
82-83	163818.0
84-85	162528.0
86-87	164186.0
88-89	172378.0
90-91	180232.0
92-93	183258.0
94-95	200989.0
96-97	303114.0
98-99	1502302.0
100-101	1.1928275E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.58807475163418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.86340811420438	69.92879492926637
2	7.392206921235045	11.766630340536043
3	2.253002152962067	5.379363112966131
4	0.9830860872571443	3.129677159996526
5	0.520641891188511	2.0718442877371697
6	0.3132661424033628	1.495934949525295
7	0.18255237054701992	1.017029419922897
8	0.12456927447016565	0.7931382982630704
9	0.08389251851409849	0.6009159631142632
>10	0.2789891130804847	3.279195307067733
>50	0.0033236580428522974	0.176648965048686
>100	9.125218720134685E-4	0.13587584033387454
>500	2.7162271072949784E-5	0.013283786746328276
>1k	1.0850841557612581E-4	0.1533101004891165
>5k	1.3563536121334414E-5	0.058357538986550966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.6508544260494587E-5	0.0	0.0	0.0
5	0.0	3.7111961964692423E-5	0.0	0.0	0.0
6	0.0	3.7111961964692423E-5	0.0	0.0	0.0
7	0.0	3.7111961964692423E-5	0.0	0.0	0.0
8	0.0	5.3017088520989174E-5	0.0	0.0	0.0
9	0.0	7.422392392938485E-5	0.0	0.0	0.0
10-11	0.0	1.1133588589407726E-4	0.0	1.0603417704197835E-5	0.0
12-13	0.0	1.219393035982751E-4	0.0	2.3857689834445126E-5	0.0
14-15	0.0	1.5640041113691808E-4	0.0	4.771537966889025E-5	0.0
16-17	0.0	1.6435297441506644E-4	0.0	7.68747783554343E-5	0.0
18-19	0.0	1.7760724654531374E-4	0.0	1.537495567108686E-4	0.0
20-21	0.0	1.9616322752765995E-4	0.0	2.703871514570448E-4	0.0
22-23	0.0	2.120683540839567E-4	0.0	4.029298727595177E-4	0.0
24-25	0.0	2.173700629360556E-4	0.0	6.096965179913756E-4	0.0
26-27	0.0	2.200209173621051E-4	0.0	8.03208891092986E-4	0.0
28-29	0.0	2.438786071965502E-4	0.0	0.0017469130667665932	0.0
30-31	2.6508544260494586E-6	2.968956957175394E-4	0.0	0.00548461780749633	0.0
32-33	5.301708852098917E-6	2.995465501435888E-4	0.0	0.014436553204265352	0.0
34-35	5.301708852098917E-6	3.393093665343307E-4	0.0	0.02653240195032903	0.0
36-37	5.301708852098917E-6	3.896756006292704E-4	0.0	0.043868989896692495	0.0
38-39	5.301708852098917E-6	3.923264550553199E-4	0.0	0.07132919089613884	0.0
40-41	5.301708852098917E-6	4.347401258721112E-4	0.0	0.10847296311394386	0.0
42-43	1.0603417704197835E-5	4.692012334107542E-4	0.0	0.1310290834251987	0.0
44-45	1.0603417704197835E-5	5.513777206182874E-4	2.6508544260494586E-6	0.15714530123063797	0.0
46-47	1.0603417704197835E-5	6.41506771103969E-4	5.301708852098917E-6	0.1825139780879313	0.0
48-49	1.0603417704197835E-5	7.236832583115022E-4	5.301708852098917E-6	0.20282482470032223	0.0
50-51	1.0603417704197835E-5	8.085105999450848E-4	5.301708852098917E-6	0.22085593650631064	0.0
52-53	1.0603417704197835E-5	8.270665809274312E-4	5.301708852098917E-6	0.24158031640916533	0.0
54-55	1.0603417704197835E-5	8.297174353534806E-4	5.301708852098917E-6	0.2767518529339895	0.0
56-57	1.0603417704197835E-5	8.456225619097773E-4	5.301708852098917E-6	0.3125516419577875	0.0
58-59	1.0603417704197835E-5	8.66829397318173E-4	5.301708852098917E-6	0.3411729171958435	0.0
60-61	1.0603417704197835E-5	8.906870871526181E-4	5.301708852098917E-6	0.35690573821444704	0.0
62-63	1.0603417704197835E-5	9.224973402652116E-4	5.301708852098917E-6	0.36612540990824705	0.0
64-65	1.0603417704197835E-5	0.0010338332261592888	5.301708852098917E-6	0.3750375824886253	0.0
66-67	1.0603417704197835E-5	0.0010550400615676847	5.301708852098917E-6	0.3806176310554594	0.0
68-69	1.0603417704197835E-5	0.0010815486058281792	5.301708852098917E-6	0.3860439300655827	0.0
70-71	1.0603417704197835E-5	0.0010895011691063276	5.301708852098917E-6	0.39121839790523116	0.0
72-73	1.0603417704197835E-5	0.001116009713366822	5.301708852098917E-6	0.39515756758234066	0.0
74-75	1.0603417704197835E-5	0.0011504708209054651	5.301708852098917E-6	0.39825376555196645	0.0
76-77	1.0603417704197835E-5	0.0011796302195920092	5.301708852098917E-6	0.4010424644081705	0.0
78-79	1.0603417704197835E-5	0.0012061387638525036	5.301708852098917E-6	0.4036827154165158	0.0
80-81	1.0603417704197835E-5	0.0012087896182785532	5.301708852098917E-6	0.4048862033259422	0.0
82-83	1.0603417704197835E-5	0.0012087896182785532	5.301708852098917E-6	0.4052917840531278	0.0
84-85	1.0603417704197835E-5	0.00122469474483485	5.301708852098917E-6	0.40562844256523606	0.0
86-87	1.0603417704197835E-5	0.0012352981625390477	5.301708852098917E-6	0.4057079681980176	0.0
88-89	1.0603417704197835E-5	0.001248552434669295	5.301708852098917E-6	0.40574508015998223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28415	0.0	51.938667	1
GTATCAA	51285	0.0	41.054462	1
CTTATAC	27145	0.0	34.86182	1
ATCAACG	62270	0.0	33.173508	3
TCAACGC	64345	0.0	32.16609	4
TATCAAC	65320	0.0	31.926708	2
CAACGCA	65095	0.0	30.277433	5
TATACAC	34415	0.0	29.870298	3
AACGCAG	68020	0.0	29.141289	6
TTATACA	37100	0.0	26.360985	2
ACGCAGA	75830	0.0	26.03422	7
CGCAGAG	76105	0.0	25.950382	8
GCAGAGT	85775	0.0	22.750948	9
ACACATC	41190	0.0	21.565626	6
TACACAT	45360	0.0	20.734913	5
TACATGG	55755	0.0	20.639786	2
GAGTACT	54315	0.0	20.510263	12-13
GTACATG	59545	0.0	19.912663	1
AGAGTAC	80695	0.0	19.48241	10-11
CAGAGTA	85610	0.0	19.279974	10-11
>>END_MODULE