FastQCFastQC Report
Fri 27 May 2016
SRR2139960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9162561
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127350.052.1244351
GTATCAA223250.041.3849531
CTTATAC102400.039.6504821
TATACAC133200.033.5707323
ATCAACG276300.032.8888473
TCAACGC280700.032.3717654
TATCAAC292600.031.2099462
CAACGCA290100.029.786255
TTATACA145450.029.5815472
AACGCAG302100.028.8253946
ACGCAGA337750.025.6984397
CGCAGAG345650.025.1164118
ACATCTC164000.022.7501728
TACACAT181500.022.2879565
ACACATC172300.021.9514926
GCAGAGT397050.021.6817789
ATACACA230400.020.5760124
GAGTACT245350.020.30391912-13
TACATGG250100.020.018132
GTACATG261900.019.7212561
Produced by FastQC (version 0.11.5)