##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9162561
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.798526198079337	33.0	31.0	34.0	30.0	34.0
2	31.480524822699678	33.0	31.0	34.0	29.0	34.0
3	31.813509345258385	33.0	31.0	34.0	30.0	34.0
4	32.95322715996107	37.0	35.0	37.0	26.0	37.0
5	34.423535843308436	37.0	35.0	37.0	28.0	37.0
6	35.12045311349087	37.0	35.0	37.0	32.0	37.0
7	35.27080387241078	37.0	35.0	37.0	32.0	37.0
8	35.41959436886696	37.0	35.0	37.0	32.0	37.0
9	37.16959865260379	39.0	37.0	39.0	33.0	39.0
10-11	37.04658653841432	39.0	37.0	39.0	33.0	39.0
12-13	36.9361196613043	39.0	37.0	39.0	32.0	39.0
14-15	38.28312657345474	40.0	38.0	41.0	32.0	41.0
16-17	38.277494196218726	40.0	38.0	41.0	32.5	41.0
18-19	38.21143504528919	40.0	38.0	41.0	32.5	41.0
20-21	38.276036814750995	40.0	38.0	41.0	32.5	41.0
22-23	38.31720761586658	40.0	38.0	41.0	32.5	41.0
24-25	38.19705122163066	40.0	38.0	41.0	32.0	41.0
26-27	38.10611069685916	40.0	38.0	41.0	32.0	41.0
28-29	38.115550634577716	40.0	38.0	41.0	32.0	41.0
30-31	38.064316725582685	40.0	37.5	41.0	32.0	41.0
32-33	37.95693217455572	40.0	37.0	41.0	32.0	41.0
34-35	37.84332289777861	40.0	37.0	41.0	31.0	41.0
36-37	37.79525030850843	40.0	37.0	41.0	31.0	41.0
38-39	37.71765175721019	40.0	37.0	41.0	31.0	41.0
40-41	37.646936876901194	40.0	37.0	41.0	31.0	41.0
42-43	37.6289219981612	40.0	37.0	41.0	31.0	41.0
44-45	37.5664104416472	40.0	36.5	41.0	31.0	41.0
46-47	37.43564552537801	40.0	36.0	41.0	30.0	41.0
48-49	37.604902217836354	40.0	37.0	41.0	31.0	41.0
50-51	37.55817355740102	40.0	36.0	41.0	31.0	41.0
52-53	37.49984113801398	40.0	36.0	41.0	31.0	41.0
54-55	37.36639618135782	39.0	36.0	41.0	30.0	41.0
56-57	37.22915653055256	39.0	35.5	41.0	30.0	41.0
58-59	37.11783717799048	39.0	35.0	41.0	30.5	41.0
60-61	36.891832818732	39.0	35.0	41.0	30.5	41.0
62-63	36.705581629909474	38.5	35.0	40.5	30.0	41.0
64-65	36.44187771765081	38.0	35.0	40.0	30.0	41.0
66-67	36.21371179867836	37.5	35.0	40.0	30.0	41.0
68-69	35.95743216962878	37.0	34.0	40.0	30.0	41.0
70-71	35.70691121543541	36.5	34.0	39.0	30.0	41.0
72-73	35.40329026065339	36.0	34.0	39.0	30.0	41.0
74-75	35.22835632045686	36.0	34.0	39.0	30.0	40.0
76-77	35.03633011734562	35.0	34.0	37.5	30.0	40.0
78-79	34.78199227803115	35.0	34.0	37.0	30.0	39.0
80-81	34.53350797221617	35.0	34.0	37.0	30.0	39.0
82-83	34.287570306708616	35.0	34.0	36.0	30.0	38.5
84-85	34.08058073126184	35.0	34.0	36.0	30.0	37.0
86-87	33.90182635087763	35.0	34.0	36.0	29.0	37.0
88-89	33.767163913718	35.0	34.0	35.0	29.0	36.5
90-91	33.637645244902515	35.0	34.0	35.0	29.0	36.0
92-93	33.59878236915328	35.0	34.0	35.0	29.0	36.0
94-95	33.570914760876335	35.0	34.0	35.0	29.0	36.0
96-97	33.5812737763155	35.0	34.0	35.0	30.0	36.0
98-99	33.61508472515758	35.0	34.0	35.0	30.0	35.0
100-101	33.22844711637259	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	5.0
17	71.0
18	452.0
19	1592.0
20	4547.0
21	9746.0
22	17617.0
23	28639.0
24	45518.0
25	70279.0
26	107262.0
27	152516.0
28	171322.0
29	142014.0
30	167547.0
31	206424.0
32	257751.0
33	337535.0
34	460692.0
35	652576.0
36	1000362.0
37	1741950.0
38	2296104.0
39	1228017.0
40	62020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.133408115918684	20.59054231671691	14.755143239974064	24.520906327390342
2	14.18429739852954	20.643950883953284	42.11506559510039	23.05668612241678
3	17.423017249047827	26.388723804035273	31.00538040189021	25.18287854502669
4	11.915392137407684	18.505901845507207	38.77000853538119	30.808697481703923
5	12.920419273300359	37.82388469547679	35.74163003191262	13.514065999310235
6	29.280616470591838	38.51144484967293	18.653877849443667	13.55406083029157
7	25.697831544900623	33.039699516231266	23.12360851260054	18.13886042626757
8	24.000541772705613	36.96265020713747	21.15583124893452	17.880976771222397
9	25.064370796744136	17.082140978374994	21.25642016296791	36.597068061912964
10-11	23.831348366007564	27.022141789906794	29.005031045708527	20.141478798377115
12-13	24.844200535411407	24.322835397804766	28.944216984765053	21.888747082018774
14-15	21.996343923520456	25.675590797304398	26.668387074805395	25.65967820436975
16-17	21.572916008416435	28.986191506315546	27.235619694912412	22.20527279035561
18-19	21.514277539115987	27.865841547945358	29.158223391166	21.461657521772658
20-21	22.377121478670013	27.068818805146925	29.02257974884489	21.531479967338164
22-23	21.964385188329672	27.17175429413577	28.96398099070127	21.899879526833285
24-25	21.80039886834128	27.313444038189655	29.147011912001258	21.73914518146781
26-27	21.924625262182555	27.504762963662934	29.06690670577242	21.50370506838209
28-29	21.652269604499423	27.36425871161806	29.2506021486718	21.73286953521071
30-31	22.0834378130114	27.365968652100765	28.949089618685715	21.601503916202127
32-33	21.968874239140053	27.18184864740977	29.02653647780329	21.82274063564689
34-35	21.66997414784545	27.51709733236616	29.274938219214874	21.53799030057352
36-37	22.028229652124402	27.234628402725658	28.956919931676268	21.78022201347367
38-39	21.703271197730434	27.256184927139277	29.168488860390234	21.87205501474006
40-41	21.946384100513328	27.155987088288324	29.101326316859932	21.796302494338416
42-43	22.063151149587213	27.10925371737337	28.94974523960044	21.87784989343898
44-45	21.872462946506527	27.56541036102092	28.60763867089547	21.95448802157708
46-47	22.25217987242584	27.194417125905417	28.16826346732707	22.38513953434167
48-49	22.112204009560347	27.26171189310059	28.215752235655405	22.41033186168366
50-51	21.830560464395962	27.479400019576417	28.317384889303764	22.372654626723858
52-53	22.012508334150006	27.508646717349578	28.383212501377763	22.095632447122654
54-55	22.0537135672762	27.43770099271482	28.13773062422531	22.37085481578367
56-57	22.290441941806957	27.672603884892215	27.83728336500706	22.199670808293767
58-59	21.92281031157503	27.944720561172215	28.267321173910865	21.865147953341886
60-61	22.193188133955267	28.285827672112056	27.623238731722548	21.897745462210125
62-63	21.95653401259805	28.551645909000968	27.572054383198115	21.919765695202877
64-65	21.876442810901967	28.426467559434656	27.637711276553773	22.059378353109608
66-67	22.005459499731707	28.333492604321762	27.52159984589433	22.139448050052195
68-69	22.103477683974514	28.038538645361	27.711673546509523	22.146310124154965
70-71	22.14218759423645	27.59897896846773	27.85392789275371	22.404905544542107
72-73	22.194909256809375	27.652968854132215	27.842863401043477	22.309258488014933
74-75	21.977061544100966	27.79764922844755	27.841217563814535	22.384071663636945
76-77	22.13288101123327	27.722853372768814	27.76951485335153	22.37475076264639
78-79	22.136173734066876	27.75986473830685	27.72987486844209	22.374086659184183
80-81	22.10694052601026	27.733427965964	27.75190086693738	22.407730641088357
82-83	22.216664573402475	27.686346756907277	27.85174778185448	22.24524088783577
84-85	22.374203563945166	27.63200941043727	27.720863265053946	22.272923760563618
86-87	22.05505192031661	27.79549107117505	27.828660115727782	22.320796892780557
88-89	22.186051623883138	27.660516238831374	27.78907247198979	22.3643596652957
90-91	22.20832475009313	27.78543071835799	27.807946920046312	22.19829761150257
92-93	22.272138917215585	27.78962899491559	27.71129877766045	22.226933310208377
94-95	22.10315631124898	27.792386477027627	27.728586087479222	22.37587112424417
96-97	22.00805936622783	27.81913310591434	27.91172832951726	22.261079198340568
98-99	22.365541768075474	28.315811681381533	27.873371563283	21.445274987259992
100-101	23.04454727630321	30.99773106645899	24.98704038092547	20.970681276312327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2790.0
1	2255.5
2	2616.0
3	3611.5
4	4516.0
5	7131.0
6	9470.0
7	11422.5
8	12764.0
9	12278.0
10	10468.5
11	8594.5
12	9222.5
13	13086.0
14	22669.5
15	36029.0
16	46928.0
17	52961.0
18	53911.5
19	50934.5
20	46183.5
21	41451.5
22	39521.0
23	43146.5
24	51813.5
25	64925.0
26	83103.0
27	103086.5
28	121825.0
29	140648.0
30	158152.0
31	175571.5
32	197237.0
33	220164.5
34	241031.0
35	260403.0
36	283075.0
37	300582.5
38	308068.0
39	316878.0
40	330267.0
41	342172.0
42	350262.0
43	361001.5
44	379368.5
45	399839.5
46	400484.0
47	392581.0
48	387955.5
49	378377.0
50	369340.5
51	357813.0
52	340898.5
53	322259.0
54	301256.0
55	279932.5
56	260042.0
57	238244.0
58	212719.5
59	183539.5
60	152482.5
61	120187.5
62	92294.5
63	70073.5
64	51971.5
65	38332.0
66	28527.5
67	21811.0
68	16491.5
69	12290.5
70	9241.0
71	6783.0
72	4798.0
73	3417.5
74	2432.5
75	1705.0
76	1169.0
77	772.0
78	543.0
79	392.0
80	288.0
81	210.5
82	156.0
83	104.5
84	62.5
85	42.0
86	38.0
87	31.5
88	19.5
89	11.5
90	9.0
91	7.0
92	5.5
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.775169627792928
3	0.006613871383775781
4	8.702599633443095
5	4.4747314642707423E-4
6	0.07593946714242884
7	0.026182636055574415
8	5.129570215139632E-4
9	2.2919356280411122E-4
10-11	0.29993797585631354
12-13	0.31065004642261046
14-15	7.0940864677463E-5
16-17	1.3096775017377784E-4
18-19	0.4841222885173697
20-21	2.4014390732802953E-4
22-23	1.637751079523624E-4
24-25	0.17468609147461098
26-27	0.1513144376406246
28-29	9.84058417643905E-5
30-31	1.751588129377773E-4
32-33	8.777114770375592E-5
34-35	3.080640495965516E-4
36-37	2.208974511195359E-4
38-39	0.0013437225668210468
40-41	0.001614776016318513
42-43	0.0010799701590745421
44-45	8.733838924304102E-4
46-47	0.001357238755591881
48-49	0.002410492265874869
50-51	3.322966067852635E-4
52-53	0.001545186875076929
54-55	6.099174592188757E-4
56-57	0.008863676123663572
58-59	1.506801279069361E-4
60-61	0.002665083031331154
62-63	0.0014866834629133601
64-65	0.001508352829673012
66-67	0.004777901358625446
68-69	0.002040203707666647
70-71	0.0025166634012261753
72-73	0.003649933273234135
74-75	7.555905156701588E-4
76-77	3.783348976706982E-4
78-79	8.587629479480296E-4
80-81	5.291511757094645E-4
82-83	0.0016942462984148028
84-85	2.4962781186661277E-4
86-87	4.276692167904337E-4
88-89	1.3473248050509647E-4
90-91	2.7992649273852734E-4
92-93	0.0
94-95	0.0
96-97	2.9910424261421896E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	881.0
20-21	2128.0
22-23	2850.0
24-25	3773.0
26-27	5218.0
28-29	8770.0
30-31	18900.0
32-33	23780.0
34-35	31736.0
36-37	45655.0
38-39	56172.0
40-41	57702.0
42-43	68293.0
44-45	84108.0
46-47	83524.0
48-49	75280.0
50-51	67374.0
52-53	65241.0
54-55	71193.0
56-57	75415.0
58-59	76910.0
60-61	78758.0
62-63	82020.0
64-65	88995.0
66-67	95671.0
68-69	100421.0
70-71	87830.0
72-73	75234.0
74-75	76211.0
76-77	79494.0
78-79	82041.0
80-81	81998.0
82-83	78677.0
84-85	78908.0
86-87	79564.0
88-89	83993.0
90-91	87516.0
92-93	88354.0
94-95	96064.0
96-97	149645.0
98-99	740244.0
100-101	5826020.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.04280948673038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6064499346725	75.30777800641924
2	6.889426818458534	11.580135711529632
3	1.8837778922577302	4.749539595429927
4	0.7248603719875799	2.4367720858973074
5	0.3430930248482962	1.4417250861775703
6	0.1817881141229737	0.9166790305313446
7	0.10725354786077355	0.6309722642747237
8	0.07176855699697715	0.48253049302676004
9	0.04732690007739005	0.3579737082121455
>10	0.14183969542319244	1.7504957034373485
>50	0.001572061684243669	0.09150458873632845
>100	6.706105261198079E-4	0.10704280509969033
>500	1.2935400211300086E-4	0.07425047885371176
>1k	4.311708163561925E-5	0.07260044237423623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0913979181148153E-5	0.0
3	0.0	0.0	0.0	1.0913979181148153E-5	0.0
4	0.0	1.0913979181148153E-5	0.0	1.0913979181148153E-5	2.1827958362296307E-5
5	0.0	1.0913979181148153E-5	0.0	1.0913979181148153E-5	2.1827958362296307E-5
6	0.0	1.0913979181148153E-5	0.0	1.0913979181148153E-5	2.1827958362296307E-5
7	0.0	1.0913979181148153E-5	0.0	1.0913979181148153E-5	2.1827958362296307E-5
8	0.0	1.0913979181148153E-5	0.0	2.1827958362296307E-5	2.1827958362296307E-5
9	0.0	1.0913979181148153E-5	0.0	2.1827958362296307E-5	2.1827958362296307E-5
10-11	0.0	1.0913979181148153E-5	0.0	3.274193754344446E-5	2.1827958362296307E-5
12-13	0.0	2.1827958362296307E-5	0.0	3.819892713401854E-5	2.1827958362296307E-5
14-15	0.0	6.002688549631484E-5	0.0	5.456989590574076E-5	4.911290631516669E-5
16-17	0.0	7.639785426803707E-5	0.0	8.185484385861115E-5	6.548387508688892E-5
18-19	0.0	8.185484385861115E-5	0.0	9.822581263033337E-5	9.822581263033337E-5
20-21	0.0	8.731183344918523E-5	0.0	1.637096877172223E-4	1.0368280222090745E-4
22-23	0.0	8.731183344918523E-5	0.0	2.2373657321353712E-4	1.2551076058320374E-4
24-25	0.0	9.822581263033337E-5	0.0	2.946774378910001E-4	1.582526981266482E-4
26-27	0.0	1.0913979181148152E-4	0.0	3.6016131297788906E-4	1.7462366689837046E-4
28-29	0.0	1.0913979181148152E-4	0.0	0.0011896237307451487	1.855376460795186E-4
30-31	0.0	1.0913979181148152E-4	0.0	0.003999973369890798	1.855376460795186E-4
32-33	0.0	1.0913979181148152E-4	0.0	0.011301425442078912	1.855376460795186E-4
34-35	0.0	1.145967814020556E-4	0.0	0.02131500134078234	1.9099463567009268E-4
36-37	0.0	1.5279570853607414E-4	0.0	0.035306722651014276	1.9645162526066675E-4
38-39	0.0	1.9645162526066675E-4	0.0	0.05365312165452432	1.9645162526066675E-4
40-41	0.0	2.346505523946853E-4	0.0	0.07181944000154541	2.073656044418149E-4
42-43	0.0	2.4010754198525936E-4	0.0	0.08789027434578607	2.1827958362296304E-4
44-45	0.0	2.564785107569816E-4	0.0	0.10363914630418286	2.346505523946853E-4
46-47	0.0	2.7284947952870383E-4	0.0	0.11740167405161067	2.4556453157583346E-4
48-49	0.0	2.83763458709852E-4	0.0	0.12891592208772198	2.619355003475557E-4
50-51	0.0	2.946774378910001E-4	0.0	0.1381928043916979	2.619355003475557E-4
52-53	0.0	2.946774378910001E-4	0.0	0.1453523747345311	2.83763458709852E-4
54-55	0.0	3.165053962532964E-4	0.0	0.15220089667070158	3.0559141707214827E-4
56-57	0.0	3.2741937543444456E-4	0.0	0.15843277878313716	3.165053962532964E-4
58-59	0.0	3.2741937543444456E-4	0.0	0.1640862199989719	3.3287636502501864E-4
60-61	0.0	3.874462609307594E-4	0.0	0.16955412356872712	3.492473337967409E-4
62-63	0.0	3.929032505213335E-4	0.0	0.17491288734667088	3.6016131297788906E-4
64-65	0.0	3.929032505213335E-4	0.0	0.1801024844473068	3.6561830256846313E-4
66-67	0.0	3.929032505213335E-4	0.0	0.1853630224126202	3.710752921590372E-4
68-69	0.0	3.929032505213335E-4	0.0	0.19043256574226353	3.710752921590372E-4
70-71	0.0	4.0381722970248164E-4	0.0	0.19508192087343268	4.0381722970248164E-4
72-73	0.0	4.147312088836298E-4	0.0	0.19904369531618943	4.911290631516669E-4
74-75	0.0	4.147312088836298E-4	0.0	0.20215417938281666	4.911290631516669E-4
76-77	0.0	4.2564518806477794E-4	0.0	0.20482264729260738	4.911290631516669E-4
78-79	0.0	4.2564518806477794E-4	0.0	0.207594798004619	4.911290631516669E-4
80-81	0.0	4.31102177655352E-4	0.0	0.20901907228775884	5.075000319233892E-4
82-83	0.0	4.5293013601764836E-4	0.0	0.20941743252787076	5.402419694668335E-4
84-85	0.0	4.5838712560822243E-4	0.0	0.20968482501780888	5.456989590574077E-4
86-87	0.0	4.693011047893706E-4	0.0	0.2098103357783921	5.566129382385558E-4
88-89	1.0913979181148153E-5	4.802150839705187E-4	0.0	0.20985399169511668	5.566129382385558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12735	0.0	52.124435	1
GTATCAA	22325	0.0	41.384953	1
CTTATAC	10240	0.0	39.650482	1
TATACAC	13320	0.0	33.570732	3
ATCAACG	27630	0.0	32.888847	3
TCAACGC	28070	0.0	32.371765	4
TATCAAC	29260	0.0	31.209946	2
CAACGCA	29010	0.0	29.78625	5
TTATACA	14545	0.0	29.581547	2
AACGCAG	30210	0.0	28.825394	6
ACGCAGA	33775	0.0	25.698439	7
CGCAGAG	34565	0.0	25.116411	8
ACATCTC	16400	0.0	22.750172	8
TACACAT	18150	0.0	22.287956	5
ACACATC	17230	0.0	21.951492	6
GCAGAGT	39705	0.0	21.681778	9
ATACACA	23040	0.0	20.576012	4
GAGTACT	24535	0.0	20.303919	12-13
TACATGG	25010	0.0	20.01813	2
GTACATG	26190	0.0	19.721256	1
>>END_MODULE