FastQCFastQC Report
Fri 27 May 2016
SRR2139959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746781
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187500.054.122521
GTATCAA338750.040.3039171
CTTATAC157050.038.973421
ATCAACG406800.032.9338073
TCAACGC411050.032.659994
TATCAAC427800.031.5624182
CAACGCA426400.030.1082255
TATACAC224400.029.9875033
AACGCAG442350.029.248996
TTATACA228150.027.9413852
ACGCAGA500100.025.798787
CGCAGAG507950.025.4176928
ACATCTC247750.023.1264158
GCAGAGT589800.021.6346869
ACACATC261050.021.4770816
TACACAT290950.021.1693345
GTACATG378200.020.3761221
TACATGG369750.020.0274522
GAGTACT379400.019.9168412-13
CATCTCC288500.019.3670949
Produced by FastQC (version 0.11.5)