##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14746781
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.794325012353543	33.0	31.0	34.0	30.0	34.0
2	31.46833346206199	33.0	31.0	34.0	29.0	34.0
3	31.799457047609238	33.0	31.0	34.0	30.0	34.0
4	32.89540103701275	37.0	35.0	37.0	26.0	37.0
5	34.39298888347226	37.0	35.0	37.0	28.0	37.0
6	35.13840288263588	37.0	35.0	37.0	32.0	37.0
7	35.27453964360087	37.0	35.0	37.0	32.0	37.0
8	35.411913623725745	37.0	35.0	37.0	32.0	37.0
9	37.175328703938845	39.0	37.0	39.0	33.0	39.0
10-11	37.0447505798045	39.0	37.0	39.0	33.0	39.0
12-13	36.939439325775574	39.0	37.0	39.0	32.0	39.0
14-15	38.292026680263305	40.0	38.0	41.0	32.0	41.0
16-17	38.28711743939236	40.0	38.0	41.0	32.5	41.0
18-19	38.21413527467452	40.0	38.0	41.0	32.5	41.0
20-21	38.28290568933565	40.0	38.0	41.0	32.5	41.0
22-23	38.328455651850064	40.0	38.0	41.0	32.5	41.0
24-25	38.2066293763887	40.0	38.0	41.0	32.0	41.0
26-27	38.110340935806335	40.0	38.0	41.0	32.0	41.0
28-29	38.12064130148488	40.0	38.0	41.0	32.0	41.0
30-31	38.065066011416434	40.0	37.5	41.0	32.0	41.0
32-33	37.96661625382493	40.0	37.0	41.0	32.0	41.0
34-35	37.865830515395835	40.0	37.0	41.0	31.0	41.0
36-37	37.81916141192802	40.0	37.0	41.0	31.0	41.0
38-39	37.74734198553205	40.0	37.0	41.0	31.0	41.0
40-41	37.66862321392982	40.0	37.0	41.0	31.0	41.0
42-43	37.64423983479095	40.0	37.0	41.0	31.0	41.0
44-45	37.58344792461144	40.0	37.0	41.0	30.5	41.0
46-47	37.45175161666005	40.0	36.0	41.0	30.0	41.0
48-49	37.62664127119559	40.0	37.0	41.0	31.0	41.0
50-51	37.583096589483446	40.0	36.0	41.0	30.5	41.0
52-53	37.52959388719083	40.0	36.0	41.0	30.5	41.0
54-55	37.40119651707286	39.5	36.0	41.0	30.0	41.0
56-57	37.27108563157961	39.0	35.5	41.0	30.0	41.0
58-59	37.17330869192847	39.0	35.0	41.0	30.5	41.0
60-61	36.965266513634916	39.0	35.0	41.0	30.5	41.0
62-63	36.792716914905284	39.0	35.0	41.0	30.0	41.0
64-65	36.540692025681395	38.0	35.0	40.0	30.0	41.0
66-67	36.32965428751299	38.0	35.0	40.0	30.0	41.0
68-69	36.08536542894298	37.0	34.0	40.0	30.0	41.0
70-71	35.8422905664431	37.0	34.0	39.5	30.0	41.0
72-73	35.539247162066964	36.0	34.0	39.0	30.0	41.0
74-75	35.35958243621695	36.0	34.0	39.0	30.0	40.5
76-77	35.160045703344196	35.5	34.0	38.5	30.0	40.0
78-79	34.89789838675984	35.0	34.0	37.0	30.0	39.0
80-81	34.63620996373892	35.0	34.0	37.0	30.0	39.0
82-83	34.37933087809223	35.0	34.0	36.5	30.0	39.0
84-85	34.157584021488574	35.0	34.0	36.0	30.0	37.0
86-87	33.96247232519171	35.0	34.0	36.0	29.5	37.0
88-89	33.81792355394727	35.0	34.0	35.5	29.0	37.0
90-91	33.67564479138962	35.0	34.0	35.0	29.0	36.0
92-93	33.62724361830048	35.0	34.0	35.0	29.0	36.0
94-95	33.59843217615146	35.0	34.0	35.0	29.0	36.0
96-97	33.60223505510944	35.0	34.0	35.0	30.0	36.0
98-99	33.6300613800976	35.0	34.0	35.0	30.0	35.5
100-101	33.24101324125087	34.5	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	18.0
17	111.0
18	708.0
19	2546.0
20	6760.0
21	15212.0
22	27430.0
23	45075.0
24	70942.0
25	111339.0
26	169912.0
27	247126.0
28	281572.0
29	229082.0
30	267786.0
31	330168.0
32	411384.0
33	538763.0
34	735561.0
35	1039184.0
36	1582045.0
37	2727497.0
38	3746494.0
39	2058275.0
40	101789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83465272861921	20.720583020796198	14.92985486120666	24.514909389377927
2	13.905637098054779	20.990566444990087	42.2846347758416	22.819161681113528
3	17.20918584045471	26.521231132597205	31.244106400239957	25.025476626708134
4	11.795789860539493	18.89283765138215	39.05992709141081	30.251445396667542
5	12.79993772177801	37.799815660802864	35.98058498129212	13.419661636127012
6	28.818134925748783	38.82253032790162	19.04192448173852	13.317410264611082
7	25.401570243073696	33.199776731889635	23.45075529344366	17.947897731593017
8	23.6478187786115	37.3851844196651	21.453340179614518	17.51365662210888
9	24.896141388894936	17.229906750180767	21.430641136669543	36.44331072425475
10-11	23.51706314890587	27.11705755775928	29.379312329100603	19.986566964234246
12-13	24.544513871992894	24.602936369988022	29.336155318673534	21.51639443934555
14-15	21.66521798745569	26.04161142856678	26.905543222154606	25.387627361822922
16-17	21.2554987924297	29.287930728921786	27.53593577382639	21.92063470482212
18-19	21.276939353133486	28.15789801013039	29.430693469977303	21.13446916675882
20-21	21.987761346424836	27.352863431567087	29.334429322038407	21.324945899969666
22-23	21.658293562288826	27.525903258209716	29.224146220638524	21.591656958862938
24-25	21.626018686465454	27.61890783779004	29.307813425758976	21.447260049985527
26-27	21.656712833561137	27.717171348995684	29.21629005148584	21.409825765957343
28-29	21.461400892527426	27.563227712424975	29.334237472728404	21.64113392231919
30-31	21.657917930756476	27.514517691884393	29.39813648218454	21.429427895174587
32-33	21.229892037372885	27.46538632709657	29.579998050797844	21.724723584732704
34-35	21.38961536602062	27.563053175392678	29.32836612602418	21.718965332562522
36-37	21.48128202581763	27.615987024794343	29.3932364381173	21.50949451127073
38-39	21.40875543521593	27.57391498972582	29.373749164817227	21.64358041024102
40-41	21.64435663619986	27.455623882964076	29.319743629700724	21.580275851135344
42-43	21.71258999444835	27.49607582626274	29.141599361342273	21.64973481794664
44-45	21.600101330458084	27.780172685864436	28.92469416160246	21.695031822075027
46-47	21.89825296816582	27.57734652841424	28.483917563742693	22.040482939677247
48-49	21.774576902406807	27.682310872463233	28.49076475313886	22.052347471991098
50-51	21.61638270995053	27.87343905346895	28.518354394665124	21.99182384191539
52-53	21.756252017779502	27.849489951140022	28.648961361062746	21.745296670017726
54-55	21.728125873470834	27.83264543242475	28.420807941457216	22.018420752647195
56-57	21.905465509440024	28.060292173539104	28.150533139031413	21.88370917798946
58-59	21.61777423507999	28.301844851432705	28.481655629370863	21.598725284116444
60-61	21.86890879728947	28.50747029712885	28.012176008090584	21.61144489749109
62-63	21.655105373217044	28.73826352346723	27.96724053908014	21.63939056423559
64-65	21.64892790166453	28.61113855074328	27.993459667952376	21.74647387963982
66-67	21.70734622374492	28.543495127742922	27.951243883924775	21.797914764587375
68-69	21.76892929322806	28.343695572130912	28.049106029738844	21.83826910490218
70-71	21.802399279390507	28.00303465354431	28.173022433470894	22.02154363359428
72-73	21.895550688013387	27.960677591832585	28.1539539992074	21.98981772094663
74-75	21.749709999964885	28.097690587005925	28.125165867830376	22.027433545198814
76-77	21.80991582764914	28.05790671729136	28.10203786549507	22.030139589564428
78-79	21.801930204290418	28.085882460706852	28.06647955397604	22.045707781026692
80-81	21.81036734253073	28.085329720338166	28.059145822511777	22.045157114619325
82-83	21.842969217064397	28.03789326198074	28.158411971749413	21.96072554920545
84-85	21.938870127071056	28.05160675571592	28.07180009772507	21.937723019487958
86-87	21.762233939368024	28.043666305053577	28.19250645961548	22.001593295962927
88-89	21.83178777572751	28.04921938745678	28.11294702402008	22.00604581279563
90-91	21.87210910639718	28.066878603325705	28.139483009207826	21.921529281069287
92-93	21.880503377767894	28.076089573802072	28.118136679893663	21.925270368536374
94-95	21.74251926299062	28.108633321137148	28.168831151297002	21.980016264575227
96-97	21.764213687853797	28.15133223778862	28.16497478389787	21.919479290459716
98-99	22.024616393302036	28.682604702831515	28.217650212497126	21.075128691369322
100-101	22.609258501147462	31.28950725165604	25.45713267134436	20.64410157585214
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5875.0
1	4458.5
2	4197.0
3	5485.5
4	6802.0
5	10467.5
6	13903.5
7	17177.0
8	19669.0
9	19497.5
10	16937.0
11	14167.5
12	15089.5
13	20468.0
14	34254.5
15	53512.5
16	69158.5
17	78384.0
18	80899.0
19	77636.0
20	71870.0
21	66807.5
22	65977.0
23	73220.5
24	88241.0
25	109623.0
26	137747.0
27	169041.0
28	202895.0
29	239028.0
30	273573.0
31	308939.0
32	344414.0
33	378916.0
34	412990.5
35	444638.5
36	477669.5
37	505501.5
38	525063.0
39	542778.5
40	560613.5
41	576997.0
42	588507.0
43	597089.0
44	604961.5
45	616199.0
46	634142.0
47	641958.0
48	630155.5
49	606531.5
50	576401.5
51	544941.5
52	512380.0
53	480467.0
54	447467.5
55	410896.0
56	373618.0
57	334898.5
58	296048.5
59	256475.5
60	213771.0
61	172716.0
62	136153.0
63	105176.5
64	79495.5
65	59374.5
66	44737.5
67	34489.0
68	26626.0
69	19852.5
70	14563.5
71	10550.5
72	7473.0
73	5118.5
74	3494.5
75	2420.5
76	1660.5
77	1119.5
78	798.5
79	591.5
80	437.5
81	323.0
82	255.5
83	198.0
84	140.0
85	95.0
86	66.0
87	48.5
88	34.5
89	24.5
90	15.5
91	11.5
92	8.5
93	5.0
94	4.0
95	3.5
96	3.0
97	2.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	1.8226486173491012
3	0.006184400514254603
4	8.886712293347273
5	3.5940046848190123E-4
6	0.07410430791641918
7	0.026907567149739325
8	6.035215414130039E-4
9	3.3905704573764265E-4
10-11	0.3049241729432342
12-13	0.3184728924909104
14-15	1.017171137212928E-4
16-17	1.3901338875243352E-4
18-19	0.5057951291200432
20-21	2.3737322786049575E-4
22-23	2.1029526234961473E-4
24-25	0.17839901717847598
26-27	0.1662819646662536
28-29	1.0869225554092739E-4
30-31	1.0541863696082829E-4
32-33	1.1245384408642549E-4
34-35	4.373364664846687E-4
36-37	2.0918415128965974E-4
38-39	0.001537371060327233
40-41	0.001768431950114407
42-43	0.0011759907495241598
44-45	8.755921411631761E-4
46-47	0.001376686272145755
48-49	0.00248393148658396
50-51	2.9224704313642123E-4
52-53	0.0016501810910183187
54-55	4.5407002924320844E-4
56-57	0.01036945493028452
58-59	1.6010625097622925E-4
60-61	0.002626500176866042
62-63	0.001448593163861332
64-65	0.0015008615404689702
66-67	0.004785881888303939
68-69	0.00176838236401658
70-71	0.002410470418540784
72-73	0.003338720869260569
74-75	5.852640521697111E-4
76-77	3.4232189114261665E-4
78-79	6.463476909249326E-4
80-81	4.8257348117277006E-4
82-83	0.0015976966931756781
84-85	2.634091956065216E-4
86-87	4.765953675703129E-4
88-89	1.258837363855654E-4
90-91	2.3711089333987995E-4
92-93	0.0
94-95	0.0
96-97	3.65384429179089E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1311.0
20-21	3278.0
22-23	4469.0
24-25	6056.0
26-27	8331.0
28-29	13311.0
30-31	28939.0
32-33	36151.0
34-35	48347.0
36-37	70100.0
38-39	85306.0
40-41	88469.0
42-43	103448.0
44-45	125303.0
46-47	124811.0
48-49	114637.0
50-51	103559.0
52-53	100577.0
54-55	107185.0
56-57	115184.0
58-59	120214.0
60-61	122336.0
62-63	124075.0
64-65	132659.0
66-67	141462.0
68-69	149354.0
70-71	133756.0
72-73	117521.0
74-75	117510.0
76-77	122022.0
78-79	126372.0
80-81	127592.0
82-83	123930.0
84-85	122985.0
86-87	125427.0
88-89	130071.0
90-91	136392.0
92-93	138085.0
94-95	152719.0
96-97	238906.0
98-99	1199152.0
100-101	9555469.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.81319203844792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.2392923991609	74.79429951226079
2	7.278638850663177	12.200919115382817
3	1.8904799241982924	4.753414707949859
4	0.6931143780492182	2.3236851388819404
5	0.3428043003320181	1.4365761327666604
6	0.18330380494077533	0.9217966202927641
7	0.10818040304477179	0.6346861426631709
8	0.07017981589336374	0.4705595509354732
9	0.046304566869965605	0.3492840199796623
>10	0.14479730757026754	1.8622802886458738
>50	0.0023804049955141763	0.13602548208730408
>100	4.6693456573303085E-4	0.06283322747695065
>500	2.8463446817337658E-5	0.01762713393446385
>1k	2.844626918184879E-5	0.03601292674228891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.781140914752853E-6	0.0	0.0	0.0
3	0.0	6.781140914752853E-6	0.0	1.3562281829505706E-5	0.0
4	0.0	2.7124563659011413E-5	0.0	2.034342274425856E-5	6.781140914752853E-6
5	0.0	2.7124563659011413E-5	0.0	2.7124563659011413E-5	6.781140914752853E-6
6	0.0	2.7124563659011413E-5	0.0	2.7124563659011413E-5	2.034342274425856E-5
7	6.781140914752853E-6	2.7124563659011413E-5	0.0	3.390570457376427E-5	2.034342274425856E-5
8	6.781140914752853E-6	3.390570457376427E-5	0.0	3.390570457376427E-5	2.034342274425856E-5
9	6.781140914752853E-6	8.137369097703424E-5	0.0	6.781140914752854E-5	2.034342274425856E-5
10-11	3.051513411638784E-5	8.137369097703424E-5	0.0	7.459255006228139E-5	2.034342274425856E-5
12-13	3.390570457376427E-5	8.476426143441066E-5	0.0	9.832654326391638E-5	2.034342274425856E-5
14-15	3.390570457376427E-5	1.4918510012456278E-4	0.0	1.0849825463604565E-4	2.7124563659011413E-5
16-17	3.390570457376427E-5	1.4918510012456278E-4	0.0	1.1527939555079851E-4	3.390570457376427E-5
18-19	4.407741594589355E-5	1.6613795241144492E-4	0.0	1.2884167738030423E-4	3.390570457376427E-5
20-21	5.4249127318022826E-5	1.7970023424095061E-4	0.0	1.6274738195406848E-4	3.390570457376427E-5
22-23	5.4249127318022826E-5	2.2038707972946774E-4	0.0	2.136059388147149E-4	3.390570457376427E-5
24-25	5.763969777539925E-5	2.6107392521798487E-4	0.0	2.9497962979174914E-4	3.390570457376427E-5
26-27	6.442083869015211E-5	2.78026777504867E-4	0.0	4.272118776294298E-4	3.390570457376427E-5
28-29	7.120197960490496E-5	2.8480791841961985E-4	0.0	0.0011799185191669966	3.390570457376427E-5
30-31	8.137369097703424E-5	3.3227590482288983E-4	0.0	0.00414666766937137	3.390570457376427E-5
32-33	8.81548318917871E-5	3.3227590482288983E-4	0.0	0.01133806760946677	3.390570457376427E-5
34-35	1.017171137212928E-4	3.831344616835362E-4	0.0	0.021306344754153467	3.390570457376427E-5
36-37	1.0849825463604565E-4	4.407741594589355E-4	0.0	0.03523141762259845	3.390570457376427E-5
38-39	1.1866996600817494E-4	4.712892935753233E-4	0.0	0.05386260228588192	4.068684548851712E-5
40-41	1.3901338875243352E-4	5.289289913507226E-4	0.0	0.07132404014137052	4.068684548851712E-5
42-43	1.4918510012456278E-4	5.560535550097341E-4	0.0	0.08574752686704984	4.068684548851712E-5
44-45	1.5596624103931563E-4	6.238649641572625E-4	0.0	0.10056771033624219	4.068684548851712E-5
46-47	1.5935681149669204E-4	6.848952323900382E-4	0.0	0.11449278320468717	4.7467986403269976E-5
48-49	1.6274738195406848E-4	6.882858028474147E-4	0.0	0.1261393927257752	5.4249127318022826E-5
50-51	1.6274738195406848E-4	7.967840574834603E-4	0.0	0.13499217218998505	5.4249127318022826E-5
52-53	1.6274738195406848E-4	8.001746279408368E-4	0.0	0.14200726246629689	5.763969777539925E-5
54-55	1.6952852286882133E-4	8.069557688555896E-4	0.0	0.1483883160670793	6.103026823277568E-5
56-57	1.7970023424095061E-4	8.137369097703424E-4	0.0	0.15422687839468152	6.103026823277568E-5
58-59	1.898719456130799E-4	8.137369097703424E-4	0.0	0.15991286505170177	6.103026823277568E-5
60-61	2.3055879110159703E-4	8.205180506850953E-4	0.0	0.1652937003675582	6.103026823277568E-5
62-63	2.3733993201634988E-4	8.272991915998482E-4	0.0	0.1702201992421261	6.103026823277568E-5
64-65	2.8480791841961985E-4	9.357974462358938E-4	0.0	0.17527893036453177	6.103026823277568E-5
66-67	2.9837020024912555E-4	9.561408689801524E-4	0.0	0.18023933494367347	6.103026823277568E-5
68-69	3.187136229933841E-4	9.900465735539167E-4	0.0	0.18524381693876107	6.103026823277568E-5
70-71	3.2549476390813695E-4	0.0010069994258407988	0.0	0.1899736627268012	7.459255006228139E-5
72-73	3.2549476390813695E-4	0.0010137805667555515	0.0	0.1936863373776284	8.137369097703424E-5
74-75	3.2549476390813695E-4	0.001040905130414563	0.0	0.19690737931213598	8.137369097703424E-5
76-77	3.2549476390813695E-4	0.0010849825463604564	0.0	0.19974189621450267	8.137369097703424E-5
78-79	3.2549476390813695E-4	0.0010917636872752093	0.0	0.20199323499820063	8.137369097703424E-5
80-81	3.2549476390813695E-4	0.0010917636872752093	0.0	0.20306465526273157	8.137369097703424E-5
82-83	3.2549476390813695E-4	0.0011019353986473387	0.0	0.2036240993881987	8.137369097703424E-5
84-85	3.2549476390813695E-4	0.0011188882509342208	0.0	0.20400723384988223	8.137369097703424E-5
86-87	3.2888533436551336E-4	0.0011324505327637265	0.0	0.2041530283795494	8.476426143441066E-5
88-89	3.3227590482288983E-4	0.0011866996600817493	0.0	0.20420049636595267	8.81548318917871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18750	0.0	54.12252	1
GTATCAA	33875	0.0	40.303917	1
CTTATAC	15705	0.0	38.97342	1
ATCAACG	40680	0.0	32.933807	3
TCAACGC	41105	0.0	32.65999	4
TATCAAC	42780	0.0	31.562418	2
CAACGCA	42640	0.0	30.108225	5
TATACAC	22440	0.0	29.987503	3
AACGCAG	44235	0.0	29.24899	6
TTATACA	22815	0.0	27.941385	2
ACGCAGA	50010	0.0	25.79878	7
CGCAGAG	50795	0.0	25.417692	8
ACATCTC	24775	0.0	23.126415	8
GCAGAGT	58980	0.0	21.634686	9
ACACATC	26105	0.0	21.477081	6
TACACAT	29095	0.0	21.169334	5
GTACATG	37820	0.0	20.376122	1
TACATGG	36975	0.0	20.027452	2
GAGTACT	37940	0.0	19.91684	12-13
CATCTCC	28850	0.0	19.367094	9
>>END_MODULE