FastQCFastQC Report
Fri 27 May 2016
SRR2139958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266661
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132100.047.0506131
CTTATAC97250.045.775581
GTATCAA237050.036.646811
TATACAC131750.036.0403023
TTATACA140000.033.0695882
ATCAACG290450.029.4266053
TCAACGC293100.029.1466164
TATCAAC300100.028.693142
CAACGCA297750.028.6202345
AACGCAG305050.028.0326236
TACACAT191750.024.9624655
ACGCAGA344850.024.7481357
CGCAGAG347450.024.5751428
ACACATC185100.024.46216
ACATCTC186600.024.4245018
ATACACA237650.021.693254
CACATCT207100.021.6792767
GCAGAGT393300.021.6132139
CATCTCC214400.021.0004529
GAGTACT245700.020.12848712-13
Produced by FastQC (version 0.11.5)