FastQCFastQC Report
Fri 27 May 2016
SRR2139957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9763713
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC187100.056.6967121
TTATACA213400.049.783842
TATACAC224650.048.263193
TACACAT326250.033.3070375
ATACACA354650.031.0769214
ACACATC361500.029.486916
ACATCTC383200.027.7651988
CACATCT389150.027.1566337
CATCTCC421950.024.998489
TACATGG208850.018.2516672
GTACATG212800.018.1372591
ACATGGG210150.017.4754473
AGAGTAC349000.017.12995110-11
CATGGGG154450.017.0025564
ATCTCCG372000.016.8890047
CCGTCTT400550.016.82339146-47
GAGTACT248950.016.79791812-13
TGCCGTC399150.016.70037844-45
TATGCCG395300.016.65544942-43
CGTATGC398750.016.36195440-41
Produced by FastQC (version 0.11.5)