##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9763713
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80728499496042	34.0	31.0	34.0	31.0	34.0
2	32.93935032707332	34.0	31.0	34.0	31.0	34.0
3	33.014378853618496	34.0	31.0	34.0	31.0	34.0
4	36.35823656430704	37.0	37.0	37.0	35.0	37.0
5	36.32747070709678	37.0	37.0	37.0	35.0	37.0
6	36.39295296779002	37.0	37.0	37.0	35.0	37.0
7	36.37683379263606	37.0	37.0	37.0	35.0	37.0
8	36.369300900180086	37.0	37.0	37.0	35.0	37.0
9	38.124785519607144	39.0	39.0	39.0	37.0	39.0
10-11	38.05711433754761	39.0	38.0	39.0	35.0	39.0
12-13	38.03465029133896	39.0	38.0	39.0	35.0	39.0
14-15	39.40274504176843	41.0	39.0	41.0	36.0	41.0
16-17	39.42437083105577	41.0	39.0	41.0	36.5	41.0
18-19	39.47950436478418	41.0	39.5	41.0	36.5	41.0
20-21	39.45177378847001	41.0	39.0	41.0	36.5	41.0
22-23	39.38940405269818	41.0	39.0	41.0	36.0	41.0
24-25	39.262546437228224	41.0	39.0	41.0	36.0	41.0
26-27	39.19315949842239	40.0	39.0	41.0	36.0	41.0
28-29	39.05515901044423	40.5	39.0	41.0	36.0	41.0
30-31	39.01852510569058	40.0	39.0	41.0	35.5	41.0
32-33	38.88938622367125	40.0	38.5	41.0	35.0	41.0
34-35	38.75700100188526	40.0	38.0	41.0	35.0	41.0
36-37	38.81829506874847	40.0	38.0	41.0	35.0	41.0
38-39	38.7831208926751	40.0	38.0	41.0	35.0	41.0
40-41	38.9215660779263	40.0	38.0	41.0	35.0	41.0
42-43	38.89541228358853	40.0	38.0	41.0	35.0	41.0
44-45	38.831515884723714	40.0	38.0	41.0	35.0	41.0
46-47	38.76994849717355	40.0	38.0	41.0	35.0	41.0
48-49	38.677467122917506	40.0	38.0	41.0	35.0	41.0
50-51	38.588314492355295	40.0	38.0	41.0	35.0	41.0
52-53	38.560718464364186	40.0	38.0	41.0	34.5	41.0
54-55	38.43162504686993	40.0	38.0	41.0	34.0	41.0
56-57	38.19616118312205	40.0	37.0	41.0	34.0	41.0
58-59	38.009225150741095	40.0	37.0	41.0	34.0	41.0
60-61	37.710753401988256	39.0	36.0	41.0	33.0	41.0
62-63	37.48254465893996	39.0	36.0	40.5	33.0	41.0
64-65	37.213690681114386	38.5	35.5	40.0	33.0	41.0
66-67	36.80591312673806	38.0	35.0	40.0	32.0	41.0
68-69	36.503287321918606	37.0	35.0	39.5	32.0	41.0
70-71	36.121593886727496	37.0	35.0	39.0	31.5	40.5
72-73	35.75833882640751	36.0	35.0	39.0	31.0	40.0
74-75	35.43884757444992	36.0	34.0	38.0	31.0	40.0
76-77	34.503161471447996	35.0	33.5	37.0	30.5	39.0
78-79	34.750513405026766	35.0	34.0	37.0	31.0	39.0
80-81	34.59424653829729	35.0	34.0	36.0	31.0	39.0
82-83	34.311866940802815	35.0	34.0	36.0	31.0	37.5
84-85	34.10221490695222	35.0	34.0	36.0	31.0	37.0
86-87	33.942032724554906	35.0	34.0	35.0	31.0	37.0
88-89	33.63856658740677	35.0	34.0	35.0	30.0	36.0
90-91	33.56674318632114	35.0	33.0	35.0	30.0	36.0
92-93	33.51486941438193	35.0	33.0	35.0	30.0	36.0
94-95	33.41710559435337	35.0	33.0	35.0	30.0	35.0
96-97	33.395810563984526	34.5	33.0	35.0	30.0	35.0
98-99	33.421618520317466	34.0	33.0	35.0	30.0	35.0
100-101	32.186865803215305	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	2.0
19	10.0
20	66.0
21	283.0
22	1210.0
23	3834.0
24	9207.0
25	18195.0
26	31313.0
27	48493.0
28	68252.0
29	90941.0
30	115562.0
31	145649.0
32	184499.0
33	241576.0
34	342423.0
35	511021.0
36	923651.0
37	2129182.0
38	3197762.0
39	1629787.0
40	70790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45026736762972	20.4277574815311	14.236094681222609	22.88588046961657
2	12.505930388910603	23.644747375674875	38.27341899314082	25.575903242273696
3	21.860501796960378	25.809174995980012	32.38440101150075	19.94592219555886
4	10.960782325570916	19.059322736951426	40.509601370790406	29.470293566687257
5	11.764401031984768	39.82144082526007	35.08568976341985	13.328468379335314
6	26.07483221029711	38.16206134758202	17.60397430894609	18.159132133174786
7	23.272557255387554	34.17373109197221	23.129957772199297	19.423753880440938
8	22.803880614655636	30.817667585613705	21.23841027248758	25.140041527243074
9	21.40927837543421	20.320192402628024	24.551143153634918	33.71938606830285
10-11	24.818912181561924	24.862307913458036	27.195471193970523	23.123308711009518
12-13	28.634771725895426	23.059571147713655	27.00728099145494	21.29837613493598
14-15	23.562465132366167	24.909908742690153	28.50450510057088	23.023121024372802
16-17	20.38009502375594	29.816925867756105	28.15154945812021	21.651429650367742
18-19	20.392070147091598	30.547971918876755	27.001938402115915	22.05801953191573
20-21	22.069198326069202	26.763801233651858	25.922978590764185	25.24402184951476
22-23	21.185987541480852	26.934733818475475	27.930079494683667	23.94919914536
24-25	24.64205514480832	27.06732494756415	26.617097915350342	21.673521992277188
26-27	22.63346639982152	25.684239222210216	31.558590204657516	20.123704173310745
28-29	20.71195168814062	29.408122677688436	28.364636299755485	21.515289334415456
30-31	21.558141692671995	27.316684205697616	27.789741629152026	23.335432472478363
32-33	22.844143849861464	25.862612713176837	29.052612668787436	22.24063076817426
34-35	23.56501912867245	26.861528762243243	28.52794191848993	21.04551019059438
36-37	22.622764551561165	25.752276423535463	30.79698275725773	20.827976267645642
38-39	21.77047623456572	27.27948945418836	29.434802281279293	21.515232029966626
40-41	21.17778899616058	28.115573531181997	29.474296036797966	21.23234143585946
42-43	21.402395093992702	27.268721278744003	26.70403713497337	24.624846492289922
44-45	21.3587810201442	26.25352645055109	27.988549165801125	24.39914336350358
46-47	24.833072891513304	26.36578569196848	27.51792258014463	21.28321883637359
48-49	22.56003901121505	25.94193505433432	28.63758662192433	22.8604393125263
50-51	19.659350964210976	27.070368538826685	31.174789628274453	22.095490868687886
52-53	21.204091251092574	28.483259738477805	28.178473467315946	22.134175543113674
54-55	20.848459605438688	26.18606657801159	28.61770452967146	24.34776928687826
56-57	23.420012030023578	26.760317017453595	26.06741606277388	23.752254889748947
58-59	23.456054240492374	28.084235156127296	27.842062654569887	20.61764794881044
60-61	23.37990638797113	28.717186133476574	28.028210577850682	19.87469690070162
62-63	20.71170231079803	31.07622606457385	27.480761430580745	20.731310194047374
64-65	20.8030401242955	31.02891906684702	26.22525706107221	21.942783747785274
66-67	21.47680316414995	29.09539028867711	25.782741908708346	23.6450646384646
68-69	21.664888805405752	27.51845828616811	27.783710572754728	23.03294233567141
70-71	23.58370086634025	26.789368931817563	26.71466246889409	22.91226773294809
72-73	21.8187863313165	26.46917448734973	29.731320436189534	21.98071874514423
74-75	21.571399950684256	28.658797521443542	27.27395941578456	22.49584311208764
76-77	21.408944323804178	27.486992365576135	27.45543372937862	23.648629581241064
78-79	22.384648716045007	26.3797795606799	28.200881631683707	23.034690091591386
80-81	23.270921852879237	26.962327424293253	26.94691177548275	22.819838947344756
82-83	21.79484519947165	26.73603032996627	29.42409719983895	22.04502727072313
84-85	22.318551054145136	27.055345483852143	27.95798847679396	22.66811498520876
86-87	21.29439594665026	28.124787068272468	28.66944535465203	21.911371630425243
88-89	21.824950920830183	26.85658266359984	26.773766207047245	24.544700208522734
90-91	21.187831245676186	27.092084447560094	27.70593032355862	24.014153983205098
92-93	22.688219945268248	26.701538421023933	28.483417994628784	22.126823639079028
94-95	21.67424973025205	25.885528890338392	29.194292786057897	23.245928593351657
96-97	20.752773235570313	26.702366900132464	30.1260033439294	22.41885652036782
98-99	21.702213129787694	26.481572025141766	29.162241334356153	22.65397351071439
100-101	27.202076515219993	27.35087411126479	34.599974646140275	10.847074727374949
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2433.0
1	2349.5
2	3670.0
3	5780.5
4	8851.5
5	13291.0
6	16150.0
7	18226.0
8	19245.0
9	18318.0
10	16221.0
11	14685.0
12	16028.0
13	21036.0
14	32536.5
15	47151.0
16	57373.0
17	61249.0
18	60010.0
19	55675.5
20	50052.5
21	44307.0
22	40494.5
23	40846.5
24	45328.0
25	53635.0
26	65054.5
27	78095.5
28	91950.0
29	107151.0
30	122022.5
31	137096.0
32	151907.5
33	165738.5
34	180140.0
35	193084.5
36	207244.5
37	221556.0
38	233645.5
39	245454.0
40	259589.0
41	279588.5
42	310977.5
43	372119.5
44	477517.5
45	659779.0
46	903679.5
47	1130067.5
48	1142211.5
49	890612.5
50	620973.0
51	441699.5
52	336697.5
53	281232.0
54	239157.5
55	206839.5
56	184536.0
57	162825.0
58	140368.0
59	120881.0
60	99664.5
61	79834.0
62	63346.5
63	48380.0
64	35864.0
65	26735.0
66	20624.0
67	15946.5
68	12063.0
69	8869.0
70	6354.5
71	4538.0
72	3261.5
73	2298.5
74	1624.0
75	1054.5
76	632.0
77	399.0
78	227.0
79	151.5
80	105.0
81	69.0
82	52.0
83	38.5
84	28.5
85	18.0
86	11.0
87	8.0
88	6.5
89	4.5
90	2.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.072601580976417E-5
2	0.004516724324035334
3	3.072601580976417E-5
4	3.072601580976417E-5
5	3.072601580976417E-5
6	3.072601580976417E-5
7	3.072601580976417E-5
8	2.0484010539842784E-5
9	2.1508211066834925E-4
10-11	8.193604215937113E-4
12-13	1.5363007904882087E-5
14-15	2.0484010539842784E-5
16-17	0.0015823898142028551
18-19	1.0242005269921392E-5
20-21	0.0
22-23	7.945355637193942E-4
24-25	0.0027035463910861975
26-27	0.007225620911676157
28-29	0.3666989924212648
30-31	0.3374934052357766
32-33	0.3362614752409812
34-35	0.18727430964193603
36-37	0.04701887381632382
38-39	0.04579998078961483
40-41	0.025886135098715214
42-43	0.025644388356971182
44-45	0.014282177798645079
46-47	0.01000801141035553
48-49	0.009788958268731924
50-51	0.008139765812676211
52-53	0.007165235750655786
54-55	9.381298689887071E-4
56-57	0.004603505895179827
58-59	0.002983839298220209
60-61	0.006085856804179996
62-63	0.0017171325943753431
64-65	1.3255677785529193E-4
66-67	7.478375085767614E-4
68-69	0.001581760717170309
70-71	0.00404182829790567
72-73	0.002076274452542066
74-75	0.0014791752421564669
76-77	5.797591010129649E-4
78-79	1.5699561768141735E-4
80-81	0.001189735256169343
82-83	0.0013457663306513674
84-85	1.5275470969502064E-5
86-87	0.0037164883889140446
88-89	0.001581943551757178
90-91	0.004333906171013635
92-93	0.012954529937496715
94-95	0.0013848571753518272
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1119.0
20-21	6942.0
22-23	7751.0
24-25	9096.0
26-27	55405.0
28-29	25010.0
30-31	38719.0
32-33	55644.0
34-35	53158.0
36-37	76151.0
38-39	87450.0
40-41	88685.0
42-43	107400.0
44-45	126395.0
46-47	107996.0
48-49	97032.0
50-51	116108.0
52-53	87585.0
54-55	130987.0
56-57	127085.0
58-59	127120.0
60-61	134352.0
62-63	138342.0
64-65	153414.0
66-67	172907.0
68-69	146895.0
70-71	94125.0
72-73	101572.0
74-75	106526.0
76-77	130481.0
78-79	167422.0
80-81	124863.0
82-83	160440.0
84-85	192310.0
86-87	148967.0
88-89	141173.0
90-91	155310.0
92-93	140210.0
94-95	182867.0
96-97	180990.0
98-99	1306390.0
100-101	4151319.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.58136313468249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02637375874683	73.57364310559105
2	8.29266124750481	13.862238621612175
3	2.1118607269758822	5.2953659493373735
4	0.7855637708847363	2.626339631990707
5	0.3497594944903887	1.4616687659402077
6	0.16913492634540106	0.8481916618579647
7	0.09280297974456433	0.5429619685007712
8	0.05053794449931518	0.33792242330221534
9	0.032450673158980335	0.2441044347539067
>10	0.08735535354463107	1.0674123816063907
>50	0.0011376880536173133	0.06505575538220748
>100	3.2210701161196266E-4	0.0532848983126466
>500	3.93290391903959E-5	0.02181040181236307
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0242005269921392E-5	0.0
2	0.0	0.0	0.0	1.0242005269921392E-5	0.0
3	0.0	0.0	0.0	1.0242005269921392E-5	0.0
4	0.0	2.0484010539842784E-5	0.0	1.0242005269921392E-5	0.0
5	0.0	2.0484010539842784E-5	0.0	1.0242005269921392E-5	0.0
6	0.0	2.0484010539842784E-5	0.0	1.0242005269921392E-5	0.0
7	0.0	2.0484010539842784E-5	0.0	3.072601580976417E-5	0.0
8	0.0	2.0484010539842784E-5	0.0	3.072601580976417E-5	0.0
9	0.0	8.193604215937114E-5	0.0	5.121002634960696E-5	0.0
10-11	0.0	8.193604215937114E-5	0.0	6.145203161952835E-5	0.0
12-13	0.0	8.193604215937114E-5	0.0	7.681503952441044E-5	0.0
14-15	0.0	1.1266205796913531E-4	0.0	9.729905006425322E-5	0.0
16-17	0.0	1.1266205796913531E-4	0.0	1.4338807377889948E-4	0.0
18-19	0.0	1.1266205796913531E-4	0.0	2.1508211066834923E-4	0.0
20-21	0.0	1.1266205796913531E-4	0.0	3.3286517127244523E-4	0.0
22-23	0.0	1.2802506587401738E-4	0.0	5.121002634960695E-4	0.0
24-25	0.0	1.4850907641386017E-4	0.0	7.066983636245759E-4	0.0
26-27	0.0	1.5363007904882088E-4	0.0	9.473854874677287E-4	0.0
28-29	0.0	1.5363007904882088E-4	0.0	0.0017104148800768724	0.0
30-31	0.0	2.0484010539842783E-4	0.0	0.0029855445361820854	0.0
32-33	0.0	2.0484010539842783E-4	0.0	0.006073509125063385	0.0
34-35	0.0	2.3044511857323133E-4	0.0	0.009760631022235086	0.0
36-37	0.0	2.560501317480348E-4	0.0	0.013498962945756395	0.0
38-39	0.0	2.714131396529169E-4	0.0	0.019152549854753002	0.0
40-41	0.0	3.0726015809764176E-4	0.0	0.02474980573476504	0.0
42-43	0.0	3.1238116073260244E-4	0.0	0.030306093593697396	0.0
44-45	0.0	3.379861739074059E-4	0.0	0.037214326148259376	0.0
46-47	0.0	3.584701844472487E-4	0.0	0.04434276181612466	0.0
48-49	0.0	3.584701844472487E-4	0.0	0.050421391943823016	0.0
50-51	0.0	4.1480121343181634E-4	0.0	0.05680216122698404	0.0
52-53	0.0	4.1992221606677703E-4	0.0	0.06253768417814001	0.0
54-55	0.0	4.2504321870173777E-4	0.0	0.06906696253771491	0.0
56-57	5.121002634960696E-6	4.3016422133669845E-4	0.0	0.07628757625300948	0.0
58-59	1.0242005269921392E-5	4.404062266066198E-4	0.0	0.08253519946766152	0.0
60-61	1.0242005269921392E-5	4.557692345115019E-4	0.0	0.0882195123924679	0.0
62-63	1.0242005269921392E-5	4.762532450513447E-4	0.0	0.09364777518552625	0.0
64-65	1.0242005269921392E-5	6.247623214652049E-4	0.0	0.09904019096013986	0.0
66-67	1.0242005269921392E-5	6.350043267351263E-4	0.0	0.10455551079799252	0.0
68-69	1.0242005269921392E-5	6.759723478148118E-4	0.0	0.11010667765428991	0.0
70-71	1.0242005269921392E-5	6.913353557196939E-4	0.0	0.11499723517067739	0.0
72-73	1.0242005269921392E-5	6.964563583546547E-4	0.0	0.11863826804413444	0.0
74-75	1.0242005269921392E-5	7.169403688944974E-4	0.0	0.12174671664355557	0.0
76-77	1.0242005269921392E-5	7.681503952441044E-4	0.0	0.12441988001900507	0.0
78-79	1.0242005269921392E-5	7.783924005140257E-4	0.0	0.1274771185920766	0.0
80-81	1.0242005269921392E-5	7.783924005140257E-4	0.0	0.12868567521392732	0.0
82-83	1.0242005269921392E-5	7.783924005140257E-4	0.0	0.12904926640100955	0.0
84-85	1.0242005269921392E-5	7.783924005140257E-4	0.0	0.12927971151958276	0.0
86-87	1.0242005269921392E-5	7.886344057839471E-4	0.0	0.12939749458018684	0.0
88-89	1.0242005269921392E-5	8.500864374034755E-4	0.0	0.12943846260126654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	18710	0.0	56.696712	1
TTATACA	21340	0.0	49.78384	2
TATACAC	22465	0.0	48.26319	3
TACACAT	32625	0.0	33.307037	5
ATACACA	35465	0.0	31.076921	4
ACACATC	36150	0.0	29.48691	6
ACATCTC	38320	0.0	27.765198	8
CACATCT	38915	0.0	27.156633	7
CATCTCC	42195	0.0	24.99848	9
TACATGG	20885	0.0	18.251667	2
GTACATG	21280	0.0	18.137259	1
ACATGGG	21015	0.0	17.475447	3
AGAGTAC	34900	0.0	17.129951	10-11
CATGGGG	15445	0.0	17.002556	4
ATCTCCG	37200	0.0	16.889004	7
CCGTCTT	40055	0.0	16.823391	46-47
GAGTACT	24895	0.0	16.797918	12-13
TGCCGTC	39915	0.0	16.700378	44-45
TATGCCG	39530	0.0	16.655449	42-43
CGTATGC	39875	0.0	16.361954	40-41
>>END_MODULE