FastQCFastQC Report
Fri 27 May 2016
SRR2139956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27341722
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA377600.049.636441
GTATCAA676750.034.8957021
ATCAACG839750.027.5939463
TCAACGC845650.027.430314
CAACGCA859600.026.999615
TATCAAC879800.026.5415612
AACGCAG890850.026.1305646
GTACATG1117100.023.1882061
TACATGG1104300.023.1143552
ACGCAGA1038750.022.3473197
ACATGGG1119950.022.3368633
CGCAGAG1047200.022.1631078
CATGGGG700550.020.199454
GCAGAGT1226100.018.822879
TGGTATC179050.017.575452
GAGTACT706450.017.52982112-13
GTGGTAT197900.017.3617691
CAGAGTA1215800.017.13566610-11
AGAGTAC1147350.017.11301810-11
ATGGGGG432350.016.8358465
Produced by FastQC (version 0.11.5)