##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27341722
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.825615116706988	31.0	31.0	34.0	30.0	34.0
2	31.997821241836927	33.0	31.0	34.0	30.0	34.0
3	32.061528531377796	33.0	31.0	34.0	30.0	34.0
4	35.76024586161764	37.0	35.0	37.0	35.0	37.0
5	35.524868660430386	37.0	35.0	37.0	33.0	37.0
6	35.56202220913518	37.0	35.0	37.0	33.0	37.0
7	35.52453269036968	37.0	35.0	37.0	33.0	37.0
8	35.522148934145406	37.0	35.0	37.0	33.0	37.0
9	37.301131618557164	39.0	37.0	39.0	34.0	39.0
10-11	37.148769433761345	39.0	37.0	39.0	33.0	39.0
12-13	37.152625902640665	39.0	37.0	39.0	33.0	39.0
14-15	38.252070388982816	40.0	38.0	41.0	33.5	41.0
16-17	38.1963494654799	40.0	38.0	41.0	33.0	41.0
18-19	38.28312020727883	40.0	38.0	41.0	33.5	41.0
20-21	38.333177556531645	40.0	38.0	41.0	34.0	41.0
22-23	38.319877643082904	40.0	38.0	41.0	34.0	41.0
24-25	38.26968019674822	40.0	38.0	41.0	34.0	41.0
26-27	38.157118962901805	40.0	38.0	41.0	34.0	41.0
28-29	37.98846045218592	40.0	37.0	41.0	33.0	41.0
30-31	37.88562225756659	40.0	37.0	41.0	33.0	41.0
32-33	37.78712573624972	40.0	37.0	41.0	33.0	41.0
34-35	37.71942640258945	39.0	37.0	41.0	33.0	41.0
36-37	37.68334796478891	39.0	37.0	41.0	33.0	41.0
38-39	37.60375583153832	39.0	37.0	41.0	33.0	41.0
40-41	37.594971239182726	39.0	37.0	41.0	33.0	41.0
42-43	37.55348230915931	39.0	37.0	41.0	32.5	41.0
44-45	37.49200436016559	39.0	37.0	41.0	32.0	41.0
46-47	37.45590176045481	39.0	37.0	41.0	32.0	41.0
48-49	37.48846519213215	39.0	37.0	41.0	32.0	41.0
50-51	37.40285917130119	39.0	36.0	40.5	32.0	41.0
52-53	37.23664412192241	39.0	36.0	40.0	31.0	41.0
54-55	37.06952982362584	39.0	36.0	40.0	31.0	41.0
56-57	36.97207833565308	39.0	35.0	40.0	31.0	41.0
58-59	36.809783222260634	39.0	35.0	40.0	31.0	41.0
60-61	36.65515616395956	38.0	35.0	40.0	31.0	41.0
62-63	36.54018629231018	38.0	35.0	40.0	30.5	41.0
64-65	36.31490498908107	38.0	35.0	40.0	30.0	41.0
66-67	36.203963533585394	38.0	34.0	40.0	30.0	41.0
68-69	35.956698327589365	37.0	34.0	40.0	30.0	41.0
70-71	35.65227237535089	37.0	34.0	39.0	29.5	41.0
72-73	35.3677937287438	36.0	34.0	39.0	29.0	40.5
74-75	35.023961904447745	36.0	34.0	39.0	29.0	40.0
76-77	32.85706161853511	34.0	31.0	36.5	27.0	39.0
78-79	34.12976622198225	35.0	33.0	37.0	29.0	39.0
80-81	34.11235664750812	35.0	33.0	37.0	29.0	39.0
82-83	33.917756428290744	35.0	33.0	36.5	29.0	39.0
84-85	33.69091554002378	35.0	33.0	36.0	29.0	37.5
86-87	33.43007041296689	35.0	33.0	36.0	29.0	37.0
88-89	33.24317146044362	35.0	33.0	35.0	29.0	37.0
90-91	33.066337637442686	34.5	32.5	35.0	29.0	36.0
92-93	33.023685964589674	34.0	32.0	35.0	29.0	36.0
94-95	32.96728344506548	34.0	32.0	35.0	29.0	36.0
96-97	32.97857678234197	34.0	32.0	35.0	29.0	35.5
98-99	33.10807825100703	34.0	32.5	35.0	29.0	35.0
100-101	31.954466621684777	33.0	31.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	2.0
18	21.0
19	53.0
20	165.0
21	877.0
22	3892.0
23	13233.0
24	34569.0
25	74293.0
26	136167.0
27	221401.0
28	328673.0
29	453535.0
30	599610.0
31	775427.0
32	997124.0
33	1312100.0
34	1784136.0
35	2494803.0
36	3821484.0
37	5849529.0
38	6144098.0
39	2272212.0
40	24317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.556524550886905	21.152359063279157	13.69830011797721	23.59281626785673
2	14.454527383491033	22.555098802866624	41.19741865544273	21.79295515819961
3	17.828621090117863	27.771836292683005	31.07156411817005	23.327978499029083
4	11.704989975939695	18.74909090377103	40.36584391286355	29.18007520742572
5	11.939374109556686	39.2383097506894	36.44018638477655	12.382129754977358
6	28.06467346862791	39.569321932246986	19.723640669011264	12.64236393011384
7	25.25656211411995	33.53370720395738	24.40676194425501	16.802968737667655
8	23.94977455283169	37.106737505659446	22.251645204189874	16.691842737318982
9	25.762441004995956	16.186262884247014	21.901853877381974	36.14944223337506
10-11	23.3558600820759	27.47115585791322	29.989095205633852	19.183888854377024
12-13	24.308258269752255	25.23091096464402	30.056908888174387	20.403921877429333
14-15	21.330315649656516	26.550274093019343	27.876329952376995	24.24308030494715
16-17	20.60414337846284	29.671354087927494	28.556523041169886	21.167979492439787
18-19	20.5423573044254	28.81382994082663	30.421262920304265	20.222549834443708
20-21	21.60449330824851	28.015840059697954	30.181692220579432	20.19797441147411
22-23	21.19491680952207	28.029585307254617	29.96491249633328	20.810585386890036
24-25	20.8202449791205	28.099669304184587	30.229630123324913	20.85045559337
26-27	20.771485734894434	28.336748404335623	30.065071214608498	20.82669464616145
28-29	20.71617460407905	28.520310632852798	29.98052065474909	20.782994108319063
30-31	20.964386149489904	28.314382971339842	29.981528195475754	20.739702683694507
32-33	20.722754778741024	28.467301316121336	29.890319540047905	20.91962436508974
34-35	20.84024240250445	28.587046321508254	29.893557179563384	20.67915409642391
36-37	20.8432351528609	28.485016170912647	29.840032483765977	20.831716192460473
38-39	20.70027051369899	28.59829562217231	29.88140094720525	20.820032916923452
40-41	20.95168065789583	28.51500837942448	29.704227323896156	20.829083638783537
42-43	20.931690416579805	28.58101949792296	29.479962445851605	21.00732763964563
44-45	20.973908392444763	28.774096604705335	29.246719192824973	21.00527581002493
46-47	20.967773715643297	28.726522020279628	29.008524819070097	21.29717944500698
48-49	21.01638058096407	28.82376333168346	28.94116638429442	21.218689703058043
50-51	21.024378338609147	28.95686983908258	28.770997930249685	21.247753892058586
52-53	21.109752564897004	28.881243529145223	28.687848622858205	21.321155283099564
54-55	21.1287622251855	28.804202235953852	28.72297453497002	21.34406100389063
56-57	21.158924010061796	28.83418703073346	28.65981737782381	21.347071581380934
58-59	21.03523457916014	28.911715441197728	28.696881249510973	21.35616873013116
60-61	21.17837413453953	28.861857660919394	28.66287572650885	21.29689247803222
62-63	20.997911632806176	28.902113666220924	28.69870034412773	21.40127435684517
64-65	20.963808913591027	28.883737678880685	28.712344103201293	21.440109304326995
66-67	21.063775580312473	28.964978086966237	28.498053263930643	21.473193068790643
68-69	21.074799461852464	29.019910504218966	28.49380287062025	21.411487163308323
70-71	21.033629220930138	28.787144192158216	28.567918290803906	21.61130829610774
72-73	21.04656780362203	28.745057074602894	28.59966614926436	21.608708972510723
74-75	21.062754356977837	28.782452598099606	28.500368487930057	21.6544245569925
76-77	21.114927422909773	28.74462077335415	28.45003981242376	21.690411991312317
78-79	21.196261073748044	28.656437281999935	28.45499783306171	21.69230381119031
80-81	21.174528699628528	28.71178769775753	28.37339459760192	21.740289005012023
82-83	21.239514324934305	28.638806779931596	28.481393275845907	21.640285619288193
84-85	21.37968107205868	28.598237700344658	28.43703865590619	21.585042571690476
86-87	21.183355010611454	28.65221931471289	28.497890507817146	21.66653516685851
88-89	21.299752875315185	28.567333509521397	28.52420391590314	21.608709699260277
90-91	21.280408064268503	28.4929514551887	28.65042458018321	21.576215900359593
92-93	21.219922089963646	28.385262402708598	28.723669701163058	21.671145806164695
94-95	21.09783745601811	28.472941423102654	28.80417344156997	21.62504767930927
96-97	21.065769295551327	28.495695246561002	28.882072184170372	21.5564632737173
98-99	21.30459388556137	29.03901191770154	28.97071920706866	20.685674989668428
100-101	24.993973123021576	36.050953950159816	29.767444514159784	9.18762841265882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5496.0
1	5141.0
2	8382.5
3	12911.5
4	17880.5
5	30447.5
6	41405.0
7	48486.5
8	53084.0
9	51610.5
10	46119.5
11	40600.0
12	43551.5
13	58495.0
14	92687.0
15	139252.5
16	176372.5
17	196554.0
18	201552.5
19	195135.5
20	183774.5
21	173912.5
22	172512.5
23	189564.0
24	227107.0
25	281804.0
26	352656.5
27	429238.5
28	505263.5
29	581566.5
30	648721.5
31	710211.0
32	772548.5
33	835757.5
34	898002.5
35	954180.0
36	1010779.0
37	1058689.0
38	1089536.5
39	1117506.0
40	1148319.5
41	1167978.0
42	1173746.0
43	1178070.5
44	1180340.5
45	1174935.5
46	1160760.5
47	1146918.0
48	1128119.0
49	1094181.0
50	1055747.0
51	1003004.5
52	933728.0
53	864134.5
54	794902.0
55	719773.0
56	642808.5
57	565413.0
58	487400.0
59	416084.0
60	349266.5
61	283315.0
62	222593.0
63	171913.0
64	129697.0
65	97153.5
66	72342.0
67	53801.0
68	40377.0
69	28671.5
70	19787.0
71	13968.5
72	9735.0
73	6796.0
74	4804.5
75	3394.0
76	2298.0
77	1508.5
78	1049.5
79	747.0
80	538.0
81	381.0
82	278.0
83	201.5
84	152.0
85	117.5
86	84.0
87	57.5
88	33.5
89	16.5
90	9.5
91	7.0
92	5.5
93	3.5
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.6574141160531148E-6
2	5.74214016220339E-4
3	2.669912304718774E-4
4	2.56018988123718E-5
5	1.4629656464212459E-5
6	0.0
7	0.0
8	0.0010350481948430315
9	0.0
10-11	2.871070081101695E-4
12-13	4.498619362745331E-4
14-15	1.8287070580265574E-6
16-17	1.8287070580265574E-6
18-19	4.2243133040413474E-4
20-21	0.0
22-23	0.0
24-25	1.609961711268552E-4
26-27	1.8303520954978153E-6
28-29	4.963542505561824E-4
30-31	3.945147621643902E-4
32-33	0.0011156254463192147
34-35	0.0012054345797804212
36-37	3.9066226406417636E-5
38-39	0.0017699581124303294
40-41	3.941949860861489E-4
42-43	1.2639062239798093E-4
44-45	1.9387698106165056E-4
46-47	4.129516016593339E-5
48-49	3.788222869684894E-5
50-51	3.838031747431009E-5
52-53	3.679233770950937E-5
54-55	4.1340237263196917E-4
56-57	6.255993670859326E-4
58-59	5.63727030825569E-4
60-61	6.551492150667994E-4
62-63	1.045053383504024E-4
64-65	8.824250859156639E-5
66-67	2.215603014965727E-4
68-69	1.3410484075516117E-4
70-71	1.5937796949741265E-4
72-73	0.0
74-75	1.1912837390317612E-5
76-77	9.389177840030286E-4
78-79	3.68819498041089E-5
80-81	2.62643363880174E-4
82-83	3.563405727059869E-5
84-85	7.765509365903192E-6
86-87	1.0534028916330736E-5
88-89	5.365041731440604E-5
90-91	8.758991823617991E-5
92-93	8.950346192398264E-5
94-95	4.121742299397521E-4
96-97	6.760849613042772E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	922.0
20-21	2680.0
22-23	5233.0
24-25	11947.0
26-27	16425.0
28-29	36087.0
30-31	84348.0
32-33	107100.0
34-35	147887.0
36-37	222560.0
38-39	258198.0
40-41	266997.0
42-43	304753.0
44-45	358359.0
46-47	355690.0
48-49	332596.0
50-51	302576.0
52-53	266764.0
54-55	288271.0
56-57	303083.0
58-59	309703.0
60-61	322209.0
62-63	295849.0
64-65	315457.0
66-67	341921.0
68-69	350291.0
70-71	344799.0
72-73	323017.0
74-75	326024.0
76-77	319698.0
78-79	343522.0
80-81	348685.0
82-83	330850.0
84-85	327346.0
86-87	341163.0
88-89	366164.0
90-91	388596.0
92-93	398413.0
94-95	436261.0
96-97	613239.0
98-99	2140190.0
100-101	1.4385849E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.70747026881618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35786270621935	72.1949744008456
2	7.546941039020923	12.332829211326214
3	2.012844972631268	4.933934122710163
4	0.8821652399994138	2.8831796047774074
5	0.4353600374984194	1.7786083660066403
6	0.2641299790352211	1.2948835445474025
7	0.15066279202229826	0.8617192919845138
8	0.09331876256352334	0.609987201414544
9	0.06502339567269663	0.4781607451833897
>10	0.1882469144336986	2.2523803881801965
>50	0.002620607229290292	0.13882744082856488
>100	7.258977124869874E-4	0.11088252064409085
>500	3.291188548250115E-5	0.018050129665482646
>1k	6.47440758981862E-5	0.1115830318858231
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.6574141160531148E-6	0.0
2	0.0	0.0	0.0	7.3148282321062295E-6	0.0
3	0.0	0.0	0.0	7.3148282321062295E-6	0.0
4	0.0	0.0	0.0	2.5601898812371804E-5	7.3148282321062295E-6
5	0.0	0.0	0.0	2.5601898812371804E-5	7.3148282321062295E-6
6	0.0	0.0	0.0	2.5601898812371804E-5	1.0972242348159343E-5
7	0.0	0.0	0.0	3.6574141160531146E-5	1.0972242348159343E-5
8	0.0	0.0	0.0	3.6574141160531146E-5	1.0972242348159343E-5
9	0.0	0.0	0.0	5.8518625856849836E-5	1.0972242348159343E-5
10-11	5.486121174079673E-6	0.0	0.0	7.680569643711541E-5	1.0972242348159343E-5
12-13	7.3148282321062295E-6	0.0	0.0	1.0057888819146066E-4	1.28009494061859E-5
14-15	7.3148282321062295E-6	0.0	0.0	1.1337983759764656E-4	1.6458363522239014E-5
16-17	9.143535290132787E-6	0.0	0.0	1.5544009993225736E-4	1.8287070580265573E-5
18-19	1.0972242348159343E-5	0.0	0.0	2.5967640223977114E-4	2.011577763829213E-5
20-21	1.28009494061859E-5	0.0	0.0	6.930799749920653E-4	2.1944484696318687E-5
22-23	1.4629656464212459E-5	0.0	0.0	0.0017189846345449639	2.3773191754345246E-5
24-25	1.4629656464212459E-5	0.0	0.0	0.003593409369022185	2.5601898812371804E-5
26-27	1.4629656464212459E-5	1.8287070580265574E-6	0.0	0.007333115302686495	2.9259312928424918E-5
28-29	2.011577763829213E-5	3.6574141160531148E-6	0.0	0.03209380886836608	2.9259312928424918E-5
30-31	2.3773191754345246E-5	3.6574141160531148E-6	0.0	0.047356197974655734	2.9259312928424918E-5
32-33	2.5601898812371804E-5	3.6574141160531148E-6	0.0	0.05440769239040613	3.2916727044478035E-5
34-35	2.5601898812371804E-5	3.6574141160531148E-6	0.0	0.063970001596827	3.2916727044478035E-5
36-37	2.5601898812371804E-5	3.6574141160531148E-6	0.0	0.07748048934152721	3.2916727044478035E-5
38-39	3.2916727044478035E-5	3.6574141160531148E-6	0.0	0.10419607075223719	3.474543410250459E-5
40-41	3.6574141160531146E-5	3.6574141160531148E-6	0.0	0.16328708191824934	3.6574141160531146E-5
42-43	3.6574141160531146E-5	3.6574141160531148E-6	0.0	0.1859392762460243	3.8402848218557705E-5
44-45	5.486121174079672E-5	3.6574141160531148E-6	0.0	0.21862375749413296	4.023155527658426E-5
46-47	6.0347332914876395E-5	3.6574141160531148E-6	0.0	0.2563408405659307	4.023155527658426E-5
48-49	6.217603997290295E-5	3.6574141160531148E-6	0.0	0.28833041313198926	4.023155527658426E-5
50-51	6.217603997290295E-5	5.486121174079673E-6	0.0	0.32208468800904344	4.023155527658426E-5
52-53	6.217603997290295E-5	7.3148282321062295E-6	0.0	0.3658694210993733	4.023155527658426E-5
54-55	6.949086820500917E-5	7.3148282321062295E-6	0.0	0.4553590296909609	4.023155527658426E-5
56-57	6.949086820500917E-5	7.3148282321062295E-6	0.0	0.5504700837789223	4.023155527658426E-5
58-59	7.314828232106229E-5	7.3148282321062295E-6	0.0	0.6524845070109337	4.023155527658426E-5
60-61	7.680569643711541E-5	7.3148282321062295E-6	0.0	0.7186562719056246	4.023155527658426E-5
62-63	7.680569643711541E-5	7.3148282321062295E-6	0.0	0.7453828255586827	4.754638350869049E-5
64-65	7.863440349514197E-5	7.3148282321062295E-6	0.0	0.7700575698926352	4.754638350869049E-5
66-67	8.046311055316853E-5	7.3148282321062295E-6	0.0	0.7800861993988528	4.754638350869049E-5
68-69	8.412052466922164E-5	7.3148282321062295E-6	0.0	0.7886463771374751	4.754638350869049E-5
70-71	8.412052466922164E-5	7.3148282321062295E-6	0.0	0.7969359062315094	4.754638350869049E-5
72-73	8.412052466922164E-5	7.3148282321062295E-6	0.0	0.8032870058440358	6.217603997290295E-5
74-75	9.692147407540754E-5	7.3148282321062295E-6	0.0	0.8085628257064423	6.217603997290295E-5
76-77	1.0240759524948722E-4	7.3148282321062295E-6	0.0	0.8133211214714274	6.217603997290295E-5
78-79	1.0240759524948722E-4	7.3148282321062295E-6	0.0	0.817421082695523	6.217603997290295E-5
80-81	1.0240759524948722E-4	7.3148282321062295E-6	0.0	0.8192625907029558	6.400474703092951E-5
82-83	1.0606500936554032E-4	7.3148282321062295E-6	0.0	0.8199757864555861	6.583345408895607E-5
84-85	1.1703725171369967E-4	7.3148282321062295E-6	0.0	0.8206743525517521	6.583345408895607E-5
86-87	1.3166690817791211E-4	7.3148282321062295E-6	0.0	0.8210711819833441	6.583345408895607E-5
88-89	1.3898173641001835E-4	7.3148282321062295E-6	0.0	0.8212467378609145	6.583345408895607E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	37760	0.0	49.63644	1
GTATCAA	67675	0.0	34.895702	1
ATCAACG	83975	0.0	27.593946	3
TCAACGC	84565	0.0	27.43031	4
CAACGCA	85960	0.0	26.99961	5
TATCAAC	87980	0.0	26.541561	2
AACGCAG	89085	0.0	26.130564	6
GTACATG	111710	0.0	23.188206	1
TACATGG	110430	0.0	23.114355	2
ACGCAGA	103875	0.0	22.347319	7
ACATGGG	111995	0.0	22.336863	3
CGCAGAG	104720	0.0	22.163107	8
CATGGGG	70055	0.0	20.19945	4
GCAGAGT	122610	0.0	18.82287	9
TGGTATC	17905	0.0	17.57545	2
GAGTACT	70645	0.0	17.529821	12-13
GTGGTAT	19790	0.0	17.361769	1
CAGAGTA	121580	0.0	17.135666	10-11
AGAGTAC	114735	0.0	17.113018	10-11
ATGGGGG	43235	0.0	16.835846	5
>>END_MODULE