FastQCFastQC Report
Fri 27 May 2016
SRR2139955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223299
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106900.049.770251
GTATCAA188500.036.862221
TCAACGC228150.029.9616364
ATCAACG229900.029.7707713
CAACGCA235850.028.9838055
TATCAAC240150.028.682362
AACGCAG241700.028.389496
ACGCAGA276300.024.7718687
CGCAGAG281850.024.2534358
GTGGTAT50850.020.976971
TGGTATC50900.020.3624612
GCAGAGT333050.020.3308229
CTTATAC53550.020.1612431
GAGTACT203500.019.8835312-13
GTACATG224550.019.2704321
TACATGG221500.019.048362
AGAGTAC300050.018.83231410-11
CAGAGTA326900.018.28288510-11
ACATGGG224100.017.9779263
AGTACTT222500.017.70040112-13
Produced by FastQC (version 0.11.5)