##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8223299
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.664361590159864	34.0	31.0	34.0	31.0	34.0
2	32.822716041335724	34.0	31.0	34.0	31.0	34.0
3	32.8701012817362	34.0	31.0	34.0	31.0	34.0
4	36.33217726851474	37.0	37.0	37.0	35.0	37.0
5	36.26770691422019	37.0	37.0	37.0	35.0	37.0
6	36.30244808075202	37.0	37.0	37.0	35.0	37.0
7	36.25891786252695	37.0	37.0	37.0	35.0	37.0
8	36.23946654499611	37.0	37.0	37.0	35.0	37.0
9	37.921923184357034	39.0	38.0	39.0	35.0	39.0
10-11	37.89518476708679	39.0	38.0	39.0	35.0	39.0
12-13	37.87065975589602	39.0	38.0	39.0	35.0	39.0
14-15	39.29510601523792	41.0	39.0	41.0	36.0	41.0
16-17	39.279734325603386	41.0	39.0	41.0	36.0	41.0
18-19	39.30285710151121	41.0	39.0	41.0	36.0	41.0
20-21	39.22126260018594	41.0	39.0	41.0	36.0	41.0
22-23	39.218303509394204	40.0	39.0	41.0	36.0	41.0
24-25	39.130105956715326	40.0	39.0	41.0	36.0	41.0
26-27	38.99526998247345	40.0	39.0	41.0	35.0	41.0
28-29	38.871199782843405	40.0	39.0	41.0	35.0	41.0
30-31	38.761356565560206	40.0	38.0	41.0	35.0	41.0
32-33	38.67042104020443	40.0	38.0	41.0	35.0	41.0
34-35	38.527602705779316	40.0	38.0	41.0	34.5	41.0
36-37	38.671057440938704	40.0	38.0	41.0	35.0	41.0
38-39	38.73331147444428	40.0	38.0	41.0	35.0	41.0
40-41	38.71651132493673	40.0	38.0	41.0	35.0	41.0
42-43	38.68037899028316	40.0	38.0	41.0	35.0	41.0
44-45	38.60859562904682	40.0	38.0	41.0	34.5	41.0
46-47	38.54388945708473	40.0	38.0	41.0	34.0	41.0
48-49	38.45895771558966	40.0	38.0	41.0	34.0	41.0
50-51	38.36588298103669	40.0	38.0	41.0	34.0	41.0
52-53	38.25755565756684	40.0	38.0	41.0	34.0	41.0
54-55	38.11878020001549	40.0	37.0	41.0	33.5	41.0
56-57	37.97409433446223	40.0	37.0	41.0	33.0	41.0
58-59	37.790196638798676	39.5	36.5	41.0	33.0	41.0
60-61	37.561396218201395	39.0	36.0	41.0	33.0	41.0
62-63	37.362966062498764	39.0	36.0	40.0	33.0	41.0
64-65	37.127289127234945	38.5	35.0	40.0	32.0	41.0
66-67	36.84571876258903	38.0	35.0	40.0	32.0	41.0
68-69	36.489961974858254	37.5	35.0	40.0	31.5	41.0
70-71	36.2057208219304	37.0	35.0	39.5	31.0	41.0
72-73	35.8733759760311	36.5	35.0	39.0	31.0	40.5
74-75	35.53740224884964	36.0	34.0	39.0	31.0	40.0
76-77	34.50402974365332	35.0	33.5	37.0	29.5	39.0
78-79	34.79129530040497	35.0	34.0	37.0	30.5	39.0
80-81	34.617920130442045	35.0	34.0	37.0	31.0	39.0
82-83	34.33601620028671	35.0	34.0	36.0	30.0	38.5
84-85	34.064072335551	35.0	34.0	36.0	30.0	37.0
86-87	33.83373985501204	35.0	34.0	35.5	30.0	37.0
88-89	33.64356026750309	35.0	33.0	35.0	30.0	36.5
90-91	33.452981864894156	35.0	33.0	35.0	30.0	36.0
92-93	33.31627636013444	34.5	33.0	35.0	29.0	36.0
94-95	33.225127416107966	34.0	33.0	35.0	29.0	35.5
96-97	33.205120674539366	34.0	33.0	35.0	29.5	35.0
98-99	33.214924336770736	34.0	33.0	35.0	30.0	35.0
100-101	32.21550378378754	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	2.0
19	9.0
20	36.0
21	209.0
22	900.0
23	3057.0
24	8122.0
25	16628.0
26	29775.0
27	47619.0
28	69342.0
29	93596.0
30	121809.0
31	153762.0
32	196210.0
33	256144.0
34	359046.0
35	526054.0
36	913912.0
37	1895563.0
38	2501783.0
39	992468.0
40	37250.0
41	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.45580709153346	21.491143639553904	15.729757607013925	24.32329166189871
2	13.48697012151815	21.744682972865164	41.5358327739023	23.23251413171439
3	16.82780840147002	26.542376727393712	31.374829007190424	25.254985863945844
4	11.808110542523274	19.066026680053024	39.28244352599693	29.843419251426766
5	12.915862599669548	37.9042279746851	35.935358789702285	13.24455063594307
6	29.22813581264648	38.72411789964101	18.91464727234167	13.133099015370838
7	25.846597575985015	33.026720784444294	23.265601798013183	17.861079841557505
8	24.14520011980593	36.91429680472521	21.318110894423274	17.622392181045587
9	25.038004820206588	17.136771993819025	21.542619330757642	36.28260385521674
10-11	23.617442759788112	27.246577991156773	29.19499491684543	19.940984332209688
12-13	24.57598829861349	24.68449706133755	29.252894732393898	21.486619907655065
14-15	21.694534934915872	26.170586136865857	27.006211601592877	25.128667326625393
16-17	21.264531600521327	29.38147573782483	27.62811020105512	21.725882460598726
18-19	21.275263127365307	28.41719606693129	29.476922826228257	20.830617979475146
20-21	22.087842336158058	27.521745456774227	29.39477549633563	20.995636710732086
22-23	21.703872506616843	27.514490164809185	29.290336900322444	21.491300428251527
24-25	21.552345046553757	27.6512274911501	29.351658268974546	21.4447691933216
26-27	21.573107613599163	27.813276185399772	29.194957692761708	21.418658508239353
28-29	21.400067585730007	27.7948268704937	29.35260833409969	21.452497209676604
30-31	21.734548692735302	27.615065676600477	29.16379758642183	21.486588044242396
32-33	21.584669925795538	27.790201758327804	29.16433353476081	21.460794781115847
34-35	21.729993877483082	27.87900675573487	29.0924507410808	21.298548625701244
36-37	21.600946869409025	27.787050972202575	29.079411218581306	21.53259093980709
38-39	21.46442335629963	27.85618226044711	29.183230146143877	21.49616423710938
40-41	21.731969699942212	27.768246477157614	29.028400033674455	21.471383789225715
42-43	21.710716425163927	27.83656353790636	28.81218380652129	21.64053623040842
44-45	21.765833906045067	27.964676902472856	28.613150216623495	21.656338974858585
46-47	21.85285625026944	27.915621954631746	28.322768384937152	21.908753410161662
48-49	21.821059225088838	28.02239613723169	28.296383668084264	21.860160969595206
50-51	21.80001322386553	28.122756176109636	28.20966600063539	21.867564599389443
52-53	21.922444911032173	28.059530394194326	28.19940280494254	21.81862188983096
54-55	21.888870530069816	28.051533779075584	28.13199074061663	21.927604950237974
56-57	21.937652438492197	28.15479460906812	28.037431749943664	21.87012120249602
58-59	21.82251062221658	28.240150282616316	28.157000699064554	21.78033839610255
60-61	21.999776810474494	28.270084481517216	28.00382927928115	21.72630942872714
62-63	21.889513119725382	28.357927986338183	27.95440594716294	21.7981529467735
64-65	21.853913297149884	28.30695880862544	27.979851853704634	21.859276040520044
66-67	21.934117058385173	28.262452015049032	27.87206399721723	21.931366929348567
68-69	21.956114901718486	28.172285717904572	27.92934093449682	21.94225844588012
70-71	21.92682533709995	27.979574973749067	28.008675168085574	22.084924521065414
72-73	21.95236113267639	27.999296635318654	28.00651767696906	22.04182455503589
74-75	21.858102209202098	28.08998098361723	27.962233871731694	22.08968293544898
76-77	21.961470467896095	28.04128303468108	27.87644575019106	22.120800747231762
78-79	21.92638510779991	28.091423697646512	27.87683501084488	22.1053561837087
80-81	21.950612062205582	28.050641616867345	27.89841646809438	22.100329852832697
82-83	21.967520933136036	28.003083355691206	27.956861083054534	22.07253462811822
84-85	22.053961783287427	27.991391839183024	27.92076881067418	22.033877566855374
86-87	21.936656932360346	27.97505868031279	27.983458817610547	22.10482556971631
88-89	21.99230277373668	27.941334068421973	27.966642149304406	22.09972100853694
90-91	22.040006725532844	27.932965251748325	27.997779987183797	22.029248035535034
92-93	22.023878687921815	27.902669024823602	28.01904482116353	22.054407466091057
94-95	21.8864457366491	27.8881062798508	28.07313732841676	22.15231065508334
96-97	21.870148357392292	27.951292996245066	28.119866787719722	22.058691858642927
98-99	22.09638261469236	28.513110362565268	28.166585841191555	21.22392118155082
100-101	22.580606914011575	31.269268242924447	25.385214297772585	20.764910545291393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1711.0
1	1504.5
2	1901.5
3	2683.5
4	3609.0
5	5382.0
6	6961.5
7	8671.5
8	10209.5
9	10613.0
10	9869.0
11	9077.5
12	10185.5
13	14028.0
14	22995.5
15	35002.5
16	44323.5
17	49182.5
18	49546.0
19	46510.0
20	42425.5
21	38563.0
22	36909.5
23	39704.5
24	46726.5
25	58086.5
26	73403.5
27	90577.5
28	109542.5
29	130179.5
30	149283.0
31	167618.0
32	186890.0
33	206438.5
34	226127.0
35	244710.5
36	263406.0
37	279265.0
38	291080.5
39	302144.5
40	314145.5
41	324131.0
42	329968.0
43	334349.0
44	338205.5
45	340784.5
46	341380.0
47	340710.0
48	339601.5
49	332141.5
50	319732.0
51	305913.0
52	287826.0
53	268366.5
54	248744.0
55	227310.0
56	205127.5
57	182922.0
58	160766.5
59	138492.5
60	115592.5
61	94057.0
62	74163.5
63	56851.0
64	42421.0
65	31581.0
66	23925.5
67	18293.0
68	13873.5
69	10009.0
70	6997.5
71	4925.5
72	3445.0
73	2333.0
74	1573.0
75	1029.0
76	639.5
77	391.0
78	252.0
79	163.5
80	103.5
81	65.0
82	47.0
83	34.0
84	27.0
85	23.0
86	17.0
87	11.5
88	7.0
89	4.0
90	2.5
91	2.5
92	2.5
93	2.0
94	1.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008013815379934501
3	0.0
4	2.4321139241075877E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.174736076117383E-4
12-13	0.0
14-15	6.992327531809314E-4
16-17	6.870721835603934E-4
18-19	0.0
20-21	4.925161861492288E-4
22-23	0.0012709042465471538
24-25	0.004543196216995458
26-27	0.016900567355331325
28-29	0.3215581262161753
30-31	0.30897941808612245
32-33	0.3177097892921187
34-35	0.1841755518917891
36-37	0.04569923857735525
38-39	0.035772093989510555
40-41	0.03188867735969696
42-43	0.027833205845135597
44-45	0.015498575584627604
46-47	0.008468934152894226
48-49	0.006408766629340883
50-51	0.0029414172251327844
52-53	0.0019417326931546715
54-55	0.0017915329146769372
56-57	7.048177255729608E-4
58-59	0.0030202712641700506
60-61	0.0030841486201044076
62-63	5.865895514916063E-4
64-65	2.4468731170546715E-4
66-67	0.007378854096875576
68-69	0.006560968177435709
70-71	0.008700668918040397
72-73	0.00908711926907439
74-75	0.00704208237573567
76-77	0.004302162682840121
78-79	3.7636837952639975E-4
80-81	5.555631498763908E-4
82-83	6.203302150662701E-4
84-85	0.0
86-87	0.001696587815045989
88-89	0.0014034422930843625
90-91	0.0011044333654417085
92-93	0.01135989356304275
94-95	1.5098714201445296E-4
96-97	4.852904030344885E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	452.0
22-23	1037.0
24-25	2302.0
26-27	3588.0
28-29	5952.0
30-31	13847.0
32-33	18460.0
34-35	27620.0
36-37	37561.0
38-39	44709.0
40-41	47586.0
42-43	57321.0
44-45	69148.0
46-47	68573.0
48-49	59949.0
50-51	53119.0
52-53	51364.0
54-55	55222.0
56-57	58133.0
58-59	58915.0
60-61	58160.0
62-63	58605.0
64-65	63166.0
66-67	67496.0
68-69	64694.0
70-71	62871.0
72-73	61555.0
74-75	62944.0
76-77	63885.0
78-79	67681.0
80-81	69470.0
82-83	68836.0
84-85	69775.0
86-87	74416.0
88-89	79640.0
90-91	86826.0
92-93	90925.0
94-95	104069.0
96-97	159500.0
98-99	713808.0
100-101	5339981.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.67212058499304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99537745977231	79.77757705330116
2	6.611410266707743	11.592727162793244
3	1.4334671743207799	3.7702532088504195
4	0.4756960759454129	1.66821134928377
5	0.20310624723719148	0.890337769967223
6	0.0946517700532364	0.49789928386142246
7	0.05723279351204122	0.35124042619425794
8	0.036489341464956554	0.2559278355986126
9	0.0205481613071407	0.1621350788327575
>10	0.07086849721466823	0.9305462065133715
>50	8.750144684511532E-4	0.05032799588528847
>100	2.5737061897542E-4	0.04026604375996579
>500	1.982737707383993E-5	0.012550585158527148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2160569620537937E-5	0.0
2	0.0	0.0	0.0	3.6481708861613815E-5	0.0
3	0.0	1.2160569620537937E-5	0.0	3.6481708861613815E-5	0.0
4	0.0	8.512398734376556E-5	0.0	4.8642278482151747E-5	0.0
5	0.0	8.512398734376556E-5	0.0	4.8642278482151747E-5	0.0
6	1.2160569620537937E-5	8.512398734376556E-5	0.0	6.0802848102689685E-5	2.4321139241075873E-5
7	1.2160569620537937E-5	8.512398734376556E-5	0.0	7.296341772322763E-5	2.4321139241075873E-5
8	2.4321139241075873E-5	1.0944512658484144E-4	0.0	7.296341772322763E-5	2.4321139241075873E-5
9	2.4321139241075873E-5	1.8240854430806905E-4	0.0	9.728455696430349E-5	4.8642278482151747E-5
10-11	2.4321139241075873E-5	1.8240854430806905E-4	0.0	1.155254113951104E-4	5.472256329242072E-5
12-13	4.8642278482151747E-5	1.8240854430806905E-4	0.0	1.337662658259173E-4	6.0802848102689685E-5
14-15	5.472256329242072E-5	2.4321139241075874E-4	0.0	1.337662658259173E-4	6.0802848102689685E-5
16-17	1.0944512658484144E-4	2.4929167722102775E-4	0.0	1.6416768987726214E-4	6.0802848102689685E-5
18-19	1.0944512658484144E-4	2.7361281646210356E-4	0.0	1.88488829118338E-4	6.0802848102689685E-5
20-21	1.0944512658484144E-4	2.7969310127237256E-4	0.0	1.9456911392860699E-4	6.688313291295866E-5
22-23	1.155254113951104E-4	3.283353797545243E-4	0.0	2.0064939873887597E-4	7.90437025334966E-5
24-25	1.520071202567242E-4	3.526565189956002E-4	0.0	2.0672968354914495E-4	8.512398734376556E-5
26-27	1.8240854430806905E-4	3.708973734264071E-4	0.0	2.9185367089291047E-4	9.728455696430349E-5
28-29	1.8240854430806905E-4	3.83057943046945E-4	0.0	5.107439240625933E-4	9.728455696430349E-5
30-31	1.9456911392860699E-4	4.134593670982899E-4	0.0	0.0010032469936943798	9.728455696430349E-5
32-33	2.0064939873887597E-4	4.3170022152909675E-4	0.0	0.002188902531696829	9.728455696430349E-5
34-35	2.371311076004898E-4	4.8034250001124853E-4	0.0	0.0038305794304694506	1.2160569620537937E-4
36-37	2.675325316518346E-4	5.107439240625934E-4	0.0	0.006110686234320314	1.2160569620537937E-4
38-39	2.675325316518346E-4	5.229044936831313E-4	0.0	0.009710214841999543	1.2160569620537937E-4
40-41	2.7969310127237256E-4	5.83707341785821E-4	0.0	0.013808326804120828	1.2160569620537937E-4
42-43	2.7969310127237256E-4	5.958679114063589E-4	0.0	0.017614585095349203	1.2768598101564832E-4
44-45	2.9793395570317947E-4	6.870721835603934E-4	0.0	0.022071433861276357	1.337662658259173E-4
46-47	3.040142405134484E-4	8.025975949555039E-4	0.0	0.027142391393040678	1.337662658259173E-4
48-49	3.040142405134484E-4	8.329990190068487E-4	0.0	0.032170786931133115	1.4592683544645526E-4
50-51	3.040142405134484E-4	8.938018671095383E-4	0.0	0.03745455443125685	1.4592683544645526E-4
52-53	3.100945253237174E-4	8.998821519198073E-4	0.0	0.042045169463009915	1.580874050669932E-4
54-55	3.161748101339864E-4	8.998821519198073E-4	0.0	0.04686075503274294	1.88488829118338E-4
56-57	3.161748101339864E-4	9.120427215403453E-4	0.0	0.05204723797590237	1.9456911392860699E-4
58-59	3.161748101339864E-4	9.363638607814211E-4	0.0	0.057616778862108746	1.9456911392860699E-4
60-61	3.2225509494425534E-4	9.78925854453304E-4	0.0	0.06274853924197575	1.9456911392860699E-4
62-63	3.283353797545243E-4	9.85006139263573E-4	0.0	0.06737563598259044	1.9456911392860699E-4
64-65	3.283353797545243E-4	0.0010822906962278764	0.0	0.07236754981182127	1.9456911392860699E-4
66-67	3.4049594937506225E-4	0.0011005315506586832	0.0	0.07736554392586237	2.0064939873887597E-4
68-69	3.4049594937506225E-4	0.0011370132595202973	0.0	0.08246082259686775	2.0672968354914495E-4
70-71	3.4049594937506225E-4	0.001155254113951104	0.0	0.08700279535013866	2.0672968354914495E-4
72-73	3.648170886161381E-4	0.001161334398761373	0.0	0.09121643272365507	2.0672968354914495E-4
74-75	3.648170886161381E-4	0.001209976677243525	0.0	0.09435385968575385	2.0672968354914495E-4
76-77	3.648170886161381E-4	0.0013315823734489042	0.0	0.09722375411620081	2.0672968354914495E-4
78-79	3.7697765823667607E-4	0.0014167063607926698	0.0	0.09972883145803163	2.0672968354914495E-4
80-81	3.7697765823667607E-4	0.0014227866456029386	0.0	0.10080504186944923	2.1889025316968288E-4
82-83	3.7697765823667607E-4	0.0014349472152234766	0.0	0.10127322379983994	2.310508227902208E-4
84-85	3.7697765823667607E-4	0.0014349472152234766	0.0	0.10153467604668151	2.310508227902208E-4
86-87	3.7697765823667607E-4	0.0014471077848440146	0.0	0.10162588031883554	2.371311076004898E-4
88-89	3.7697765823667607E-4	0.001513990917756973	0.0	0.10165020145807661	2.4321139241075874E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10690	0.0	49.77025	1
GTATCAA	18850	0.0	36.86222	1
TCAACGC	22815	0.0	29.961636	4
ATCAACG	22990	0.0	29.770771	3
CAACGCA	23585	0.0	28.983805	5
TATCAAC	24015	0.0	28.68236	2
AACGCAG	24170	0.0	28.38949	6
ACGCAGA	27630	0.0	24.771868	7
CGCAGAG	28185	0.0	24.253435	8
GTGGTAT	5085	0.0	20.97697	1
TGGTATC	5090	0.0	20.362461	2
GCAGAGT	33305	0.0	20.330822	9
CTTATAC	5355	0.0	20.161243	1
GAGTACT	20350	0.0	19.88353	12-13
GTACATG	22455	0.0	19.270432	1
TACATGG	22150	0.0	19.04836	2
AGAGTAC	30005	0.0	18.832314	10-11
CAGAGTA	32690	0.0	18.282885	10-11
ACATGGG	22410	0.0	17.977926	3
AGTACTT	22250	0.0	17.700401	12-13
>>END_MODULE