FastQCFastQC Report
Fri 27 May 2016
SRR2139954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18868081
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241000.050.2476041
GTATCAA465150.039.476131
ATCAACG610300.029.6209093
TCAACGC612350.029.5290814
TATCAAC626000.029.099222
CAACGCA631200.028.634065
AACGCAG650200.027.8370046
ACGCAGA753300.024.0271077
CGCAGAG759800.023.810248
CTTATAC136100.023.571131
GCAGAGT865900.020.7492689
TGGTATC110400.020.5277462
AGAGTAC800300.019.80116510-11
GTGGTAT121500.019.2540631
GAGTACT560750.018.93450212-13
GTACATG512500.018.6696381
AGTACTT603050.018.59757412-13
TATACAC229000.018.4209733
TACATGG504950.018.4207742
ACATGGG514350.017.623453
Produced by FastQC (version 0.11.5)