##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18868081
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19138904481065	33.0	31.0	34.0	30.0	34.0
2	32.43044621230956	34.0	31.0	34.0	31.0	34.0
3	32.480648561981475	34.0	31.0	34.0	31.0	34.0
4	36.119378117997265	37.0	35.0	37.0	35.0	37.0
5	36.0018017730579	37.0	35.0	37.0	35.0	37.0
6	36.02935014959921	37.0	35.0	37.0	35.0	37.0
7	35.97174524531668	37.0	35.0	37.0	35.0	37.0
8	35.941455201512014	37.0	35.0	37.0	35.0	37.0
9	37.55076141553558	39.0	37.0	39.0	35.0	39.0
10-11	37.46861837724779	39.0	37.0	39.0	35.0	39.0
12-13	37.437248891394944	39.0	37.0	39.0	35.0	39.0
14-15	38.70232598111063	40.0	38.0	41.0	34.5	41.0
16-17	38.67719563001664	40.0	38.0	41.0	34.5	41.0
18-19	38.694353098229755	40.0	38.0	41.0	34.0	41.0
20-21	38.60854105858674	40.0	38.0	41.0	34.0	41.0
22-23	38.58056054948601	40.0	38.0	41.0	34.0	41.0
24-25	38.46480059772622	40.0	38.0	41.0	34.0	41.0
26-27	38.30360659147934	40.0	38.0	41.0	34.0	41.0
28-29	38.17550881472238	40.0	38.0	41.0	34.0	41.0
30-31	38.03864777636427	40.0	37.5	41.0	33.5	41.0
32-33	37.91810975972881	40.0	37.0	41.0	33.0	41.0
34-35	37.73449368059196	40.0	37.0	41.0	33.0	41.0
36-37	37.89381894764191	40.0	37.0	41.0	33.0	41.0
38-39	37.94829599507446	40.0	37.0	41.0	33.0	41.0
40-41	37.9325624619142	40.0	37.0	41.0	33.0	41.0
42-43	37.891542172887554	40.0	37.0	41.0	33.0	41.0
44-45	37.808989448499915	40.0	37.0	41.0	33.0	41.0
46-47	37.727849324760484	40.0	37.0	41.0	33.0	41.0
48-49	37.63266875920651	40.0	37.0	41.0	33.0	41.0
50-51	37.537547768765926	39.5	37.0	41.0	32.0	41.0
52-53	37.416249891152425	39.0	36.0	40.0	32.0	41.0
54-55	37.267312727328445	39.0	36.0	40.0	32.0	41.0
56-57	37.10347876570912	39.0	36.0	40.0	31.0	41.0
58-59	36.91208376782757	39.0	35.0	40.0	31.0	41.0
60-61	36.69290066057604	38.0	35.0	40.0	31.0	41.0
62-63	36.48838788714711	38.0	35.0	40.0	31.0	41.0
64-65	36.247638457785854	38.0	35.0	40.0	30.5	41.0
66-67	35.96265157388686	37.0	34.0	40.0	30.0	41.0
68-69	35.60426827689149	37.0	34.0	39.0	30.0	40.5
70-71	35.33316436251462	36.0	34.0	39.0	29.0	40.0
72-73	34.9977124432949	36.0	34.0	39.0	29.0	40.0
74-75	34.67511606886014	35.0	33.5	38.0	29.0	40.0
76-77	33.70712153038765	34.5	32.0	37.0	28.5	39.0
78-79	34.06424580792654	35.0	33.0	37.0	29.0	39.0
80-81	33.9203774183517	35.0	33.0	36.5	29.0	39.0
82-83	33.64565625975203	35.0	33.0	36.0	29.0	37.5
84-85	33.37127053481049	35.0	33.0	35.5	29.0	37.0
86-87	33.13272552788021	34.0	32.0	35.0	29.0	37.0
88-89	32.94684407717695	34.0	32.0	35.0	29.0	36.0
90-91	32.738858465758454	34.0	32.0	35.0	28.0	36.0
92-93	32.596954975097916	34.0	32.0	35.0	28.0	35.5
94-95	32.51824664741865	34.0	32.0	35.0	27.0	35.0
96-97	32.51988902980955	34.0	32.0	35.0	28.0	35.0
98-99	32.576422563041945	34.0	32.0	35.0	29.0	35.0
100-101	31.562165846837797	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	4.0
19	18.0
20	157.0
21	853.0
22	3793.0
23	12252.0
24	30855.0
25	62325.0
26	107961.0
27	167226.0
28	235189.0
29	312614.0
30	397571.0
31	495837.0
32	626973.0
33	816555.0
34	1136413.0
35	1692104.0
36	2862635.0
37	4702066.0
38	4065671.0
39	1129216.0
40	9790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.382814381898655	24.189012564554133	18.029901189198963	25.39827186434825
2	11.604035071679103	23.34405836622794	40.88592958897974	24.165976973113214
3	14.470985626040681	27.758782438848172	32.80849099688421	24.96174093822694
4	10.966581279056683	20.38711735170468	38.99349679355241	29.652804575686226
5	12.44723959444944	38.23898553742541	36.09137421991926	13.222400648205893
6	28.956599132322904	39.735142088391726	18.38752636132168	12.920732417963693
7	25.190847462531824	33.38240209827402	23.41519769977643	18.01155273941772
8	24.095285577796705	37.44681825353622	21.03244097796697	17.425455190700102
9	24.77340965411374	16.782464523021712	21.444782858415756	36.99934296444879
10-11	23.49613933874574	27.638267365237652	29.14456801115055	19.721025284866055
12-13	24.41294109347951	24.974105739741102	29.358833577193145	21.254119589586242
14-15	21.483915494541773	26.439882867982256	27.137380809066553	24.938820828409415
16-17	21.04231793097792	29.689843052616084	27.71474745729497	21.553091559111024
18-19	21.001343485858474	28.543543988389704	29.90405595566396	20.551056570087862
20-21	21.796113731275064	27.619782044666362	29.78725101434478	20.796853209713795
22-23	21.38720046345022	27.680056403617904	29.739039179889442	21.19370395304243
24-25	21.298879834394675	27.853094832791648	29.754097678364438	21.09392765444924
26-27	21.332250314686984	27.93129117113843	29.640952108011614	21.09550640616297
28-29	21.10768418836045	27.94745139108377	29.83116340279618	21.113701017759603
30-31	21.448468530407467	27.7840714740409	29.65105537318411	21.116404622367526
32-33	21.422363821153017	27.86188682763086	29.63910922340996	21.07664012780616
34-35	21.46490927558705	28.043249610837645	29.61597438840907	20.875866725166233
36-37	21.36716817365413	27.92701631581381	29.546114389232304	21.159701121299758
38-39	21.275571693679307	27.96885932653102	29.62462243333121	21.13094654645846
40-41	21.52319118876736	27.904725757029436	29.484471397983093	21.08761165622011
42-43	21.536606096398387	27.976978791451838	29.230278324365695	21.25613678778408
44-45	21.584320200965593	28.16535830606569	28.920643352198717	21.329678140770003
46-47	21.726928155396962	28.060431513202772	28.621302012680484	21.591338318719778
48-49	21.664667656196617	28.200665958583997	28.586518461327902	21.548147923891484
50-51	21.64873512479261	28.289971177219304	28.463442475457573	21.597851222530515
52-53	21.82513546780606	28.2788245780337	28.416488165136318	21.479551789023922
54-55	21.785204350805863	28.256082683350396	28.35598064020557	21.602732325638165
56-57	21.889576942894184	28.422946369379037	28.164825417462446	21.52265127026433
58-59	21.673087705279897	28.579024892659856	28.353267962481937	21.39461943957831
60-61	21.892422105297147	28.634731005968383	28.130184984359673	21.342661904374797
62-63	21.790497297419396	28.71090716920329	28.059626655171797	21.438968878205518
64-65	21.727389132440415	28.617799492582886	28.131056157161797	21.523755217814905
66-67	21.83638621476319	28.559457648697133	28.033613888633724	21.570542247905948
68-69	21.85929524284395	28.401678339999233	28.086961593394534	21.652064823762284
70-71	21.84561665410614	28.22457642759137	28.168200737302364	21.761606181000126
72-73	21.880718766431443	28.25645992287868	28.1352367625009	21.727584548188982
74-75	21.754857102706104	28.3736406757491	28.06969846769929	21.80180375384551
76-77	21.859803753384007	28.30397191398592	28.05603006147604	21.780194271154034
78-79	21.837215331627966	28.35893181830307	28.036511328302936	21.767341521766028
80-81	21.86212229532988	28.293563085525687	28.067418067054973	21.776896552089458
82-83	21.870038585295237	28.26173441390732	28.131505268079017	21.73672173271843
84-85	21.948341932764862	28.28385437696784	28.072970137387397	21.694833552879896
86-87	21.840202656847683	28.249734413963306	28.11779324556293	21.79226968362609
88-89	21.871730531430032	28.206884096674923	28.13474924219325	21.78663612970179
90-91	21.88754598592378	28.219079617635597	28.17469957321726	21.718674823223363
92-93	21.928473015496166	28.167392856934352	28.15232161726599	21.751812510303488
94-95	21.769525204286797	28.21350361211409	28.205229474431942	21.811741709167176
96-97	21.690376708980512	28.287852400565995	28.30529227922672	21.716478611226773
98-99	21.87526821015129	28.85493540562537	28.317165415609267	20.95263096861407
100-101	22.34957408168775	31.66522912096979	25.576343589630085	20.408853207712376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5736.0
1	4848.5
2	5642.5
3	7605.5
4	9519.5
5	13249.0
6	16579.0
7	21193.0
8	25774.5
9	27189.5
10	25738.0
11	24347.5
12	27631.0
13	38822.5
14	67126.5
15	105730.5
16	134419.0
17	146161.0
18	143945.0
19	133081.0
20	118907.0
21	105828.5
22	98646.5
23	102218.0
24	117253.5
25	144643.0
26	181690.0
27	223000.0
28	268315.0
29	316086.5
30	361778.0
31	407483.5
32	455129.5
33	502202.5
34	546549.0
35	586742.5
36	628901.0
37	664573.5
38	686328.0
39	704791.0
40	728149.5
41	749390.5
42	758478.0
43	762725.0
44	765071.0
45	767280.0
46	768937.5
47	767874.5
48	770295.5
49	754684.5
50	719898.5
51	681623.5
52	633821.0
53	583852.0
54	535771.0
55	485894.0
56	436358.0
57	385971.5
58	335428.5
59	286741.5
60	237945.5
61	192196.5
62	150383.0
63	113883.0
64	84347.0
65	62661.0
66	47343.5
67	36180.0
68	27349.5
69	19913.0
70	14416.5
71	10520.0
72	7557.5
73	5237.5
74	3545.0
75	2346.0
76	1491.5
77	909.0
78	589.5
79	396.5
80	263.5
81	180.0
82	128.5
83	92.5
84	61.5
85	40.0
86	32.5
87	23.5
88	15.5
89	11.5
90	7.5
91	4.5
92	4.0
93	3.5
94	2.5
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0599912094928996E-5
2	0.005331755783749285
3	1.0599912094928996E-5
4	2.1199824189857992E-5
5	1.0599912094928996E-5
6	1.0599912094928996E-5
7	1.0599912094928996E-5
8	0.0
9	0.0
10-11	7.128440883839751E-4
12-13	0.0
14-15	7.419938466450297E-4
16-17	7.075441323365105E-4
18-19	0.0
20-21	4.478615841638052E-4
22-23	0.001073378883822717
24-25	0.004217468808822722
26-27	0.013704264205909752
28-29	0.29569076165364905
30-31	0.28347978332529417
32-33	0.29134590117069886
34-35	0.16607885573516476
36-37	0.03869853049950514
38-39	0.02828453907573191
40-41	0.024317911897640432
42-43	0.019886751585888207
44-45	0.00882951667127057
46-47	0.003956045622003382
48-49	0.002840434834300388
50-51	9.83770125863831E-4
52-53	8.149224063707917E-4
54-55	5.327330865364219E-4
56-57	2.1814284263143758E-4
58-59	0.0014379031264122263
60-61	0.001195896511549458
62-63	1.6176398745993805E-4
64-65	6.824983737250707E-5
66-67	0.006736829386169656
68-69	0.006171010535509943
70-71	0.008200538986841128
72-73	0.008653201902113166
74-75	0.006851624628841797
76-77	0.004146286260357857
78-79	2.731050873444055E-4
80-81	3.78822450957501E-4
82-83	4.8701762000528124E-4
84-85	0.0
86-87	0.001402831745523928
88-89	0.0011318842183505719
90-91	8.785681103774174E-4
92-93	0.009906351792238333
94-95	1.0263034496182328E-4
96-97	1.0859200975648532E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	377.0
20-21	1427.0
22-23	2955.0
24-25	5891.0
26-27	9633.0
28-29	14497.0
30-31	29492.0
32-33	40167.0
34-35	60839.0
36-37	80296.0
38-39	97816.0
40-41	109069.0
42-43	135724.0
44-45	164606.0
46-47	160311.0
48-49	135820.0
50-51	119952.0
52-53	117159.0
54-55	127942.0
56-57	137177.0
58-59	139499.0
60-61	141954.0
62-63	147237.0
64-65	160360.0
66-67	167435.0
68-69	157375.0
70-71	151991.0
72-73	150776.0
74-75	156096.0
76-77	157640.0
78-79	168297.0
80-81	175156.0
82-83	174276.0
84-85	180643.0
86-87	194082.0
88-89	211324.0
90-91	233109.0
92-93	250270.0
94-95	295866.0
96-97	444518.0
98-99	1669642.0
100-101	1.1789385E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.7041936340126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7506119242307	71.69592988160439
2	8.043369014437406	13.143539588508219
3	2.2269072726679924	5.458429890331696
4	0.8860601440594242	2.8957931832644923
5	0.43009440870328314	1.75702584247996
6	0.22921869082149762	1.1236876979648707
7	0.1283317139208179	0.7339667442499851
8	0.08450470240696434	0.552351085475458
9	0.05397163544951218	0.3968738058160154
>10	0.16417778487236218	1.9817720852141254
>50	0.002071165660815704	0.11032126951715908
>100	6.108598960490337E-4	0.08802961949764332
>500	3.5385456279567495E-5	0.01812613136136977
>1k	3.5297416920792755E-5	0.0441531747146034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0599912094928996E-5	0.0
2	0.0	0.0	0.0	2.1199824189857992E-5	0.0
3	0.0	0.0	0.0	2.1199824189857992E-5	0.0
4	0.0	3.709969233225149E-5	0.0	4.2399648379715984E-5	0.0
5	0.0	3.709969233225149E-5	0.0	4.2399648379715984E-5	0.0
6	0.0	3.709969233225149E-5	0.0	4.2399648379715984E-5	0.0
7	0.0	3.709969233225149E-5	0.0	5.2999560474644985E-5	0.0
8	0.0	3.709969233225149E-5	0.0	6.359947256957398E-5	5.299956047464498E-6
9	0.0	6.359947256957398E-5	0.0	1.0599912094928997E-4	5.299956047464498E-6
10-11	1.0599912094928996E-5	6.624945059330623E-5	0.0	1.1659903304421897E-4	5.299956047464498E-6
12-13	1.0599912094928996E-5	6.889942861703847E-5	0.0	1.3779885723407697E-4	5.299956047464498E-6
14-15	1.0599912094928996E-5	7.419938466450297E-5	0.0	1.5104874735273822E-4	5.299956047464498E-6
16-17	1.0599912094928996E-5	8.479929675943197E-5	0.0	1.6959859351886396E-4	5.299956047464498E-6
18-19	1.0599912094928996E-5	9.539920885436097E-5	0.0	1.8814843968498969E-4	5.299956047464498E-6
20-21	1.3249890118661245E-5	1.1129907699675446E-4	0.0	1.934483957324542E-4	5.299956047464498E-6
22-23	2.3849802213590242E-5	1.404488352578092E-4	0.0	2.1199824189857991E-4	1.3249890118661245E-5
24-25	2.6499780237322493E-5	1.457487913052737E-4	0.0	2.464479562070992E-4	2.6499780237322493E-5
26-27	3.974967035598374E-5	1.4839876932900596E-4	0.0	3.1269740680040545E-4	2.6499780237322493E-5
28-29	4.2399648379715984E-5	1.5104874735273822E-4	0.0	5.034958245091274E-4	2.6499780237322493E-5
30-31	4.2399648379715984E-5	1.6429863747139946E-4	0.0	0.0011103407919438124	2.6499780237322493E-5
32-33	4.2399648379715984E-5	1.6959859351886394E-4	0.0	0.0023001809245995924	2.6499780237322493E-5
34-35	4.2399648379715984E-5	2.1729819794604442E-4	0.0	0.003945817277337319	3.179973628478699E-5
36-37	4.7699604427180485E-5	2.702977584206894E-4	0.0	0.006648794861544213	3.179973628478699E-5
38-39	5.2999560474644985E-5	2.755977144681539E-4	0.0	0.010644961721332445	3.179973628478699E-5
40-41	5.829951652210948E-5	2.808976705156184E-4	0.0	0.015255923482626558	3.179973628478699E-5
42-43	6.359947256957398E-5	2.9679753865801187E-4	0.0	0.01947203851838457	3.179973628478699E-5
44-45	6.359947256957398E-5	3.444971430851924E-4	0.0	0.02445664718102493	3.4449714308519244E-5
46-47	7.684936268823522E-5	3.7629687936997937E-4	0.0	0.030082550525408494	3.709969233225149E-5
48-49	9.009925280689648E-5	3.7629687936997937E-4	0.0	0.035650154353269944	3.709969233225149E-5
50-51	9.009925280689648E-5	4.054466376310341E-4	0.0	0.0411515087305381	3.709969233225149E-5
52-53	9.274923083062872E-5	4.0809661565476636E-4	0.0	0.046573363767094283	4.504962640344824E-5
54-55	9.539920885436097E-5	4.0809661565476636E-4	0.0	0.052159517441121864	5.829951652210948E-5
56-57	9.804918687809322E-5	4.0809661565476636E-4	0.0	0.05836576597270279	5.829951652210948E-5
58-59	1.0069916490182546E-4	4.1074659367849864E-4	0.0	0.06437591613052754	5.829951652210948E-5
60-61	1.0864909897302221E-4	4.186965277496954E-4	0.0	0.06991172022210419	5.829951652210948E-5
62-63	1.1659903304421896E-4	4.292964398446244E-4	0.0	0.07537067495099263	5.829951652210948E-5
64-65	1.1659903304421896E-4	4.8759595636673386E-4	0.0	0.08066533104240967	6.0949494545841726E-5
66-67	1.2189898909168345E-4	4.8759595636673386E-4	0.0	0.08674703060687516	6.359947256957398E-5
68-69	1.2719894513914796E-4	5.008458464853951E-4	0.0	0.0924921829623267	6.359947256957398E-5
70-71	1.2719894513914796E-4	5.087957805565918E-4	0.0	0.09758014076789262	7.154940664077073E-5
72-73	1.2719894513914796E-4	5.140957366040563E-4	0.0	0.10125831026483298	7.419938466450298E-5
74-75	1.351488792103447E-4	5.299956047464499E-4	0.0	0.10431638490421999	7.419938466450298E-5
76-77	1.4309881328154145E-4	5.803451871973626E-4	0.0	0.10711476169728124	7.419938466450298E-5
78-79	1.4309881328154145E-4	5.988950333634883E-4	0.0	0.1095845412153997	7.419938466450298E-5
80-81	1.4839876932900596E-4	6.015450113872206E-4	0.0	0.11086183062283864	7.949934071196747E-5
82-83	1.4839876932900596E-4	6.068449674346851E-4	0.0	0.11145807567817839	8.479929675943197E-5
84-85	1.4839876932900596E-4	6.147949015058818E-4	0.0	0.11184232249161957	8.479929675943197E-5
86-87	1.4839876932900596E-4	6.253948136008108E-4	0.0	0.11198542130490112	8.479929675943197E-5
88-89	1.4839876932900596E-4	6.49244615814401E-4	0.0	0.1120410708433995	8.479929675943197E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24100	0.0	50.247604	1
GTATCAA	46515	0.0	39.47613	1
ATCAACG	61030	0.0	29.620909	3
TCAACGC	61235	0.0	29.529081	4
TATCAAC	62600	0.0	29.09922	2
CAACGCA	63120	0.0	28.63406	5
AACGCAG	65020	0.0	27.837004	6
ACGCAGA	75330	0.0	24.027107	7
CGCAGAG	75980	0.0	23.81024	8
CTTATAC	13610	0.0	23.57113	1
GCAGAGT	86590	0.0	20.749268	9
TGGTATC	11040	0.0	20.527746	2
AGAGTAC	80030	0.0	19.801165	10-11
GTGGTAT	12150	0.0	19.254063	1
GAGTACT	56075	0.0	18.934502	12-13
GTACATG	51250	0.0	18.669638	1
AGTACTT	60305	0.0	18.597574	12-13
TATACAC	22900	0.0	18.420973	3
TACATGG	50495	0.0	18.420774	2
ACATGGG	51435	0.0	17.62345	3
>>END_MODULE