FastQCFastQC Report
Fri 27 May 2016
SRR2139953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981174
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111350.052.633991
GTATCAA204800.038.3023261
CTTATAC72500.036.7930341
ATCAACG258150.030.0046673
TCAACGC261000.029.67744
TATCAAC267150.029.202952
TATACAC103450.029.0900783
CAACGCA266500.029.0490655
AACGCAG273800.028.3370136
ACGCAGA312750.024.7942437
CGCAGAG315050.024.6132348
TTATACA114850.024.603082
ACACATC124000.021.8563926
GTGGTAT53650.021.8323211
TGGTATC50200.021.7147852
GCAGAGT361050.021.3355469
ACATCTC127100.021.1551448
TACACAT144200.020.691935
GAGTACT220350.019.8559712-13
GTACATG248050.019.6292421
Produced by FastQC (version 0.11.5)