##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139953_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7981174 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.674621427875145 34.0 31.0 34.0 31.0 34.0 2 32.82594189777093 34.0 31.0 34.0 31.0 34.0 3 32.873161517340684 34.0 31.0 34.0 31.0 34.0 4 36.332718469738914 37.0 37.0 37.0 35.0 37.0 5 36.269044128094436 37.0 37.0 37.0 35.0 37.0 6 36.30477746757557 37.0 37.0 37.0 35.0 37.0 7 36.261812610525716 37.0 37.0 37.0 35.0 37.0 8 36.24232525189903 37.0 37.0 37.0 35.0 37.0 9 37.92575465714693 39.0 38.0 39.0 35.0 39.0 10-11 37.90018273000939 39.0 38.0 39.0 35.0 39.0 12-13 37.87449365218701 39.0 38.0 39.0 35.0 39.0 14-15 39.29621976666591 41.0 39.0 41.0 36.0 41.0 16-17 39.27955335894193 41.0 39.0 41.0 36.0 41.0 18-19 39.31124806951959 41.0 39.0 41.0 36.0 41.0 20-21 39.23389742158534 41.0 39.0 41.0 36.0 41.0 22-23 39.22948645607925 40.5 39.0 41.0 36.0 41.0 24-25 39.13598856495997 40.0 39.0 41.0 36.0 41.0 26-27 39.00010770726614 40.0 39.0 41.0 35.0 41.0 28-29 38.87396623146009 40.0 39.0 41.0 35.0 41.0 30-31 38.756840581410735 40.0 38.0 41.0 35.0 41.0 32-33 38.65657299161006 40.0 38.0 41.0 35.0 41.0 34-35 38.50981623026115 40.0 38.0 41.0 34.5 41.0 36-37 38.65522630321442 40.0 38.0 41.0 35.0 41.0 38-39 38.71097248922293 40.0 38.0 41.0 35.0 41.0 40-41 38.699009781649984 40.0 38.0 41.0 35.0 41.0 42-43 38.66276761630327 40.0 38.0 41.0 35.0 41.0 44-45 38.59240311984752 40.0 38.0 41.0 34.5 41.0 46-47 38.52809492249226 40.0 38.0 41.0 34.0 41.0 48-49 38.44296164252469 40.0 38.0 41.0 34.0 41.0 50-51 38.34621582445671 40.0 38.0 41.0 34.0 41.0 52-53 38.24266757008938 40.0 37.5 41.0 34.0 41.0 54-55 38.09901974177802 40.0 37.0 41.0 33.5 41.0 56-57 37.950511592917266 40.0 37.0 41.0 33.0 41.0 58-59 37.76457555779722 39.5 36.5 41.0 33.0 41.0 60-61 37.528165530575876 39.0 36.0 41.0 33.0 41.0 62-63 37.33588207866438 39.0 35.5 40.0 33.0 41.0 64-65 37.106598444400895 38.5 35.0 40.0 32.0 41.0 66-67 36.83170877691647 38.0 35.0 40.0 32.0 41.0 68-69 36.481718978885816 37.5 35.0 40.0 31.5 41.0 70-71 36.208355199652885 37.0 35.0 39.5 31.0 41.0 72-73 35.883371334448626 36.5 35.0 39.0 31.0 40.5 74-75 35.548805571333176 36.0 34.0 39.0 31.0 40.0 76-77 34.520028258065224 35.0 33.5 37.0 29.5 39.0 78-79 34.808196427924535 35.0 34.0 37.0 30.5 39.0 80-81 34.64169341350148 35.0 34.0 37.0 31.0 39.0 82-83 34.363003919939416 35.0 34.0 36.0 30.0 38.5 84-85 34.09419633690851 35.0 34.0 36.0 30.0 37.0 86-87 33.86106402179592 35.0 34.0 35.5 30.0 37.0 88-89 33.67368522995879 35.0 33.0 35.0 30.0 36.5 90-91 33.48330821423705 35.0 33.0 35.0 30.0 36.0 92-93 33.344789509186135 35.0 33.0 35.0 30.0 36.0 94-95 33.25131515737943 34.0 33.0 35.0 29.0 35.5 96-97 33.2334818620732 34.0 33.0 35.0 30.0 35.0 98-99 33.24267294070968 34.0 33.0 35.0 30.0 35.0 100-101 32.247734043286265 33.0 31.0 34.5 27.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 1.0 15 0.0 16 0.0 17 0.0 18 4.0 19 10.0 20 32.0 21 204.0 22 922.0 23 3114.0 24 7931.0 25 16394.0 26 28833.0 27 46101.0 28 67383.0 29 90220.0 30 117519.0 31 148881.0 32 187928.0 33 246437.0 34 347393.0 35 508332.0 36 878220.0 37 1812183.0 38 2403619.0 39 1029476.0 40 40036.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.6794724685867 21.390825460013776 15.730129427074264 24.199572644325258 2 13.502514116846287 21.570568693631607 41.78659177779538 23.14032541172673 3 16.87922102688151 26.579460615693883 31.486332712455585 25.054985644969026 4 11.786746000084198 19.131986696683995 39.03115433731003 30.050112965921784 5 12.826985102692912 37.9776584246879 35.84950033666726 13.345856135951928 6 29.047969634542486 38.718614579759816 18.815815818575064 13.417599967122632 7 25.74165154149001 33.17226262702705 22.948942097992102 18.137143733490838 8 24.194711204141147 36.86294773174974 21.263488303851037 17.678852760258078 9 25.006108123942667 17.196091702799613 21.317202206091483 36.48059796716623 10-11 23.635937174916048 27.286124244724558 29.001242496110972 20.076696084248415 12-13 24.66362091592039 24.652432085805923 29.09946581793606 21.58448118033763 14-15 21.73885015331519 26.185538786440354 26.86595187447356 25.2096591857709 16-17 21.251560560263062 29.491951493451786 27.46416913541328 21.792318810871876 18-19 21.226595235237323 28.27116035811273 29.53079960416851 20.97144480248144 20-21 22.05326435938225 27.365052980935683 29.42201112043327 21.159671539248794 22-23 21.677838018551242 27.500766567218644 29.33963194498309 21.481763469247028 24-25 21.552830638224485 27.676048756234245 29.438156254022424 21.33296435151885 26-27 21.50167736267892 27.802390632875028 29.369160037182912 21.326771967263134 28-29 21.211538903198264 27.718945223463763 29.600917897330532 21.46859797600744 30-31 21.651260674080035 27.524739455903894 29.25300301293176 21.570996857084307 32-33 21.608212311223113 27.646308989168865 29.39889221530815 21.346586484299863 34-35 21.623734266435942 27.916957692555087 29.311708345870564 21.147599695138403 36-37 21.425605951037426 27.775555756009084 29.23802756089663 21.56081073205686 38-39 21.3445733436781 27.75621203288973 29.395738546551925 21.503476076880247 40-41 21.61470540195171 27.65312468974087 29.288871665615122 21.443298242692297 42-43 21.69484436982519 27.680120851212326 29.101152988295503 21.52388179066698 44-45 21.602188562137588 27.95125652548532 28.864410009399 21.582144902978094 46-47 21.868444041454485 27.74814326686953 28.38384373512872 21.99956895654726 48-49 21.77327430224836 27.893592436097776 28.40138973172415 21.93174352992971 50-51 21.654655015766128 28.02212602212602 28.408001977446425 21.915216984661427 52-53 21.808149715428534 28.032177176645632 28.440684586756575 21.718988521169255 54-55 21.77101272076203 28.01329683620336 28.2717469695074 21.943943473527206 56-57 21.9830046035325 28.223038643289726 27.949358338677122 21.844598414500652 58-59 21.660242818161294 28.47403972113395 28.28447776651013 21.581239694194636 60-61 21.96162321616581 28.57956676665902 27.885588635163572 21.5732213820116 62-63 21.73794306698234 28.739005921618688 27.84106246098649 21.68198855041249 64-65 21.77081754424701 28.607257933117342 27.88186929529588 21.74005522733977 66-67 21.879056140593466 28.431119369343662 27.82031696076748 21.86950752929539 68-69 21.87884541828712 28.304086493431583 27.934229316096758 21.88283877218453 70-71 21.88268241590508 28.0361641280596 28.01127227520045 22.069881180834873 72-73 21.914805566882244 28.054627195269976 27.999478741915908 22.031088495931876 74-75 21.767922046681516 28.170316739211348 27.99222872338516 22.06953249072198 76-77 21.929415714149307 28.12330411219178 27.866745369961894 22.08053480369702 78-79 21.90843624069071 28.17332583898488 27.85048316870497 22.067754751619443 80-81 21.897627649025445 28.1034875148338 27.8980889950151 22.100795841125652 82-83 21.9427481270489 28.073675483961946 27.963849169989174 22.019727218999975 84-85 22.065192087493067 28.012490441592508 27.932456287794672 21.989861183119753 86-87 21.91811620370662 27.98946530608041 27.994399401968415 22.09801908824455 88-89 21.96342838996178 27.983220697340656 27.9646420646116 22.088708848085965 90-91 21.99602349188553 27.96449827318578 27.999403359870705 22.040074875057982 92-93 22.016897475060393 27.921340412915757 28.02315578067335 22.038606331350508 94-95 21.851599192807868 27.970302467235282 28.103409410054216 22.074688929902642 96-97 21.84404806690857 27.971176861893838 28.14728918957603 22.037485881621567 98-99 22.071235547383754 28.589157695110657 28.16855366212866 21.171053095376934 100-101 22.51995688549485 31.312815847755836 25.423665319897626 20.743561946851685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1791.0 1 1584.5 2 1999.0 3 2813.0 4 3889.0 5 5959.0 6 7640.5 7 9397.5 8 10959.5 9 11247.5 10 10480.5 11 9800.0 12 11094.0 13 15399.0 14 25622.5 15 39211.0 16 49685.5 17 55265.5 18 55840.0 19 52472.0 20 47324.0 21 42113.0 22 39344.0 23 41336.5 24 48108.0 25 59518.5 26 74718.5 27 91870.0 28 110886.5 29 129853.0 30 147370.5 31 165846.5 32 184503.0 33 203010.0 34 221564.5 35 238914.5 36 257558.5 37 273076.5 38 282898.0 39 290095.0 40 299143.0 41 309103.5 42 314725.5 43 319017.0 44 323494.5 45 326214.0 46 329136.0 47 332904.5 48 337696.0 49 330498.0 50 314699.5 51 300519.5 52 281135.5 53 260819.0 54 241620.0 55 221628.5 56 200755.0 57 179661.0 58 158843.5 59 137716.5 60 115633.5 61 94826.5 62 75097.0 63 57395.5 64 42966.0 65 32016.5 66 24195.5 67 18609.0 68 14079.0 69 10058.5 70 7125.5 71 5085.5 72 3618.5 73 2532.0 74 1753.5 75 1189.0 76 762.0 77 449.0 78 272.5 79 184.5 80 119.5 81 73.5 82 53.5 83 40.5 84 30.0 85 18.0 86 10.0 87 7.0 88 5.0 89 2.5 90 1.5 91 2.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007993811436763563 3 0.0 4 7.517691006360718E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.830928131625748E-4 12-13 0.0 14-15 8.332107532049795E-4 16-17 8.26946010699679E-4 18-19 0.0 20-21 4.197459785046205E-4 22-23 0.0012530569891571726 24-25 0.004912831702302451 26-27 0.017507536138676234 28-29 0.32367369391725137 30-31 0.3111039122475271 32-33 0.3190027199324935 34-35 0.18485060111921997 36-37 0.04647403874086 38-39 0.03663684226572773 40-41 0.03258620280067175 42-43 0.02858875587149229 44-45 0.01627022906896458 46-47 0.008946994538460458 48-49 0.0066987939321781235 50-51 0.002752315886768121 52-53 0.0019247894576454791 54-55 0.0017495531010726997 56-57 7.04369635678141E-4 58-59 0.003305200944142035 60-61 0.003168622310179154 62-63 7.099706093255974E-4 64-65 2.910803881677668E-4 66-67 0.007552165759413699 68-69 0.0069116548215036105 70-71 0.009308818399917549 72-73 0.009896700961402275 74-75 0.007733578374581329 76-77 0.004668194833229873 78-79 4.1979980434276025E-4 80-81 5.479321811219923E-4 82-83 7.101629785015717E-4 84-85 0.0 86-87 0.001884182345099653 88-89 0.0015845754194840041 90-91 0.0011637625327799944 92-93 0.011726397693043327 94-95 1.0138896117546306E-4 96-97 8.606791912128787E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 84.0 20-21 420.0 22-23 979.0 24-25 2387.0 26-27 3807.0 28-29 6144.0 30-31 13708.0 32-33 19214.0 34-35 28488.0 36-37 39071.0 38-39 46478.0 40-41 49600.0 42-43 60863.0 44-45 73942.0 46-47 73251.0 48-49 63828.0 50-51 56015.0 52-53 54966.0 54-55 60130.0 56-57 65038.0 58-59 66279.0 60-61 67070.0 62-63 69006.0 64-65 74024.0 66-67 80912.0 68-69 72360.0 70-71 67413.0 72-73 64390.0 74-75 65652.0 76-77 67253.0 78-79 71415.0 80-81 72351.0 82-83 70881.0 84-85 72199.0 86-87 77068.0 88-89 81907.0 90-91 88512.0 92-93 91041.0 94-95 102925.0 96-97 154079.0 98-99 669820.0 100-101 5016204.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.1969022772113 #Duplication Level Percentage of deduplicated Percentage of total 1 89.9427363210376 77.5278525320946 2 7.191626078590824 12.39791780621075 3 1.7060823539861172 4.411770418302479 4 0.5782865878339967 1.993860499989959 5 0.24362572601582094 1.0499891448800185 6 0.12217144799016545 0.6318480218084215 7 0.07190491535567053 0.4338586673514718 8 0.0465822947097284 0.3212199603954172 9 0.02482260107647557 0.19256681873296477 >10 0.0707615405534614 0.8721832698646089 >50 0.0010190530357492439 0.05667087948137862 >100 3.1705770889746946E-4 0.05408373993341335 >500 4.2681546509520994E-5 0.029045762269405813 >1k 2.1340559019527296E-5 0.027132478685122002 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.264742505300599E-5 0.0 3 0.0 0.0 0.0 6.264742505300599E-5 0.0 4 0.0 0.0 0.0 1.0023588008480957E-4 0.0 5 0.0 0.0 0.0 1.2529485010601198E-4 0.0 6 0.0 0.0 0.0 1.2529485010601198E-4 0.0 7 0.0 0.0 0.0 1.6288330513781557E-4 0.0 8 0.0 0.0 0.0 1.6288330513781557E-4 0.0 9 0.0 1.2529485010601197E-5 0.0 2.756486702332263E-4 1.2529485010601197E-5 10-11 0.0 1.2529485010601197E-5 0.0 4.260024903604407E-4 1.2529485010601197E-5 12-13 0.0 1.2529485010601197E-5 0.0 4.260024903604407E-4 1.2529485010601197E-5 14-15 0.0 1.2529485010601197E-5 0.0 4.5732620288694366E-4 1.8794227515901796E-5 16-17 0.0 1.2529485010601197E-5 0.0 5.011794004240479E-4 3.758845503180359E-5 18-19 0.0 1.8794227515901796E-5 0.0 5.011794004240479E-4 3.758845503180359E-5 20-21 0.0 3.758845503180359E-5 0.0 5.638268254770539E-4 3.758845503180359E-5 22-23 0.0 3.758845503180359E-5 0.0 5.763563104876551E-4 3.758845503180359E-5 24-25 0.0 6.264742505300599E-5 0.0 6.07680023014158E-4 3.758845503180359E-5 26-27 0.0 8.770639507420838E-5 0.0 6.828569330777652E-4 5.0117940042404786E-5 28-29 0.0 9.397113757950897E-5 0.0 8.89593435752685E-4 5.0117940042404786E-5 30-31 0.0 1.0023588008480957E-4 0.0 0.0014158318061979354 5.6382682547705384E-5 32-33 0.0 1.0023588008480957E-4 0.0 0.0025372207146467426 7.517691006360718E-5 34-35 0.0 1.2529485010601198E-4 0.0 0.004404113981226321 7.517691006360718E-5 36-37 0.0 1.5035382012721437E-4 0.0 0.006922540468357162 7.517691006360718E-5 38-39 0.0 1.5035382012721437E-4 0.0 0.010731503911579926 7.517691006360718E-5 40-41 0.0 1.8794227515901796E-4 0.0 0.014734674372467008 7.517691006360718E-5 42-43 0.0 1.9420701766431855E-4 0.0 0.018806757000912395 7.517691006360718E-5 44-45 0.0 2.3179547269612213E-4 0.0 0.02398769905279599 1.0023588008480957E-4 46-47 0.0 2.693839277279257E-4 0.0 0.02972620318765134 1.0023588008480957E-4 48-49 0.0 2.756486702332263E-4 0.0 0.035038704832146245 1.0023588008480957E-4 50-51 0.0 3.1950186777033056E-4 0.0 0.04011314626143973 1.0023588008480957E-4 52-53 0.0 3.2576661027563115E-4 0.0 0.04508108706814311 1.0023588008480957E-4 54-55 0.0 3.3203135278093174E-4 0.0 0.05028708809004791 1.0650062259011018E-4 56-57 0.0 3.3829609528623233E-4 0.0 0.055724884584648825 1.1276536509541078E-4 58-59 0.0 3.3829609528623233E-4 0.0 0.06147591820451477 1.1276536509541078E-4 60-61 0.0 3.508255802968335E-4 0.0 0.06708912748926411 1.2529485010601198E-4 62-63 6.264742505300598E-6 3.508255802968335E-4 0.0 0.07245174707380142 1.3782433511661316E-4 64-65 1.2529485010601197E-5 4.260024903604407E-4 0.0 0.0774510115930313 1.3782433511661316E-4 66-67 1.2529485010601197E-5 4.385319753710419E-4 0.0 0.08290133757264281 1.3782433511661316E-4 68-69 1.2529485010601197E-5 4.635909453922443E-4 0.0 0.08887790192269959 1.5035382012721437E-4 70-71 1.2529485010601197E-5 4.886499154134467E-4 0.0 0.09434702212982701 1.7541279014841676E-4 72-73 1.2529485010601197E-5 4.886499154134467E-4 0.0 0.0984629579558095 2.2553073019082156E-4 74-75 1.2529485010601197E-5 5.074441429293485E-4 0.0 0.10167677086102872 2.2553073019082156E-4 76-77 1.2529485010601197E-5 5.262383704452503E-4 0.0 0.10477155365864721 2.2553073019082156E-4 78-79 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.1077222473786438 2.2553073019082156E-4 80-81 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.10905663753227282 2.3806021520142277E-4 82-83 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.10949516950764386 2.756486702332263E-4 84-85 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.10970190601031879 2.756486702332263E-4 86-87 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.1098272008604248 2.756486702332263E-4 88-89 1.2529485010601197E-5 5.387678554558514E-4 0.0 0.1098710540579619 2.756486702332263E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11135 0.0 52.63399 1 GTATCAA 20480 0.0 38.302326 1 CTTATAC 7250 0.0 36.793034 1 ATCAACG 25815 0.0 30.004667 3 TCAACGC 26100 0.0 29.6774 4 TATCAAC 26715 0.0 29.20295 2 TATACAC 10345 0.0 29.090078 3 CAACGCA 26650 0.0 29.049065 5 AACGCAG 27380 0.0 28.337013 6 ACGCAGA 31275 0.0 24.794243 7 CGCAGAG 31505 0.0 24.613234 8 TTATACA 11485 0.0 24.60308 2 ACACATC 12400 0.0 21.856392 6 GTGGTAT 5365 0.0 21.832321 1 TGGTATC 5020 0.0 21.714785 2 GCAGAGT 36105 0.0 21.335546 9 ACATCTC 12710 0.0 21.155144 8 TACACAT 14420 0.0 20.69193 5 GAGTACT 22035 0.0 19.85597 12-13 GTACATG 24805 0.0 19.629242 1 >>END_MODULE