FastQCFastQC Report
Fri 27 May 2016
SRR2139952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11595540
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172900.053.180831
GTATCAA311150.037.712751
ATCAACG396650.029.1803743
TCAACGC397200.029.129284
CAACGCA407150.028.4282075
TATCAAC411050.028.3276772
AACGCAG419500.027.6220136
CTTATAC87850.027.1937641
ACGCAGA485250.023.8881637
CGCAGAG489800.023.6750268
TGGTATC80600.022.762982
GTGGTAT84400.022.5017411
TATACAC136750.020.9555573
GCAGAGT558200.020.6358669
GAGTACT329200.020.22308712-13
GTACATG387200.019.4528351
AGAGTAC517250.018.90970410-11
TACATGG386850.018.8484482
CAGAGTA550900.018.5969710-11
GTACTTT360350.018.47528814-15
Produced by FastQC (version 0.11.5)