##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11595540
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61804185057358	34.0	31.0	34.0	31.0	34.0
2	32.780945432468	34.0	31.0	34.0	31.0	34.0
3	32.82693026801684	34.0	31.0	34.0	31.0	34.0
4	36.307701064374754	37.0	37.0	37.0	35.0	37.0
5	36.241129089287774	37.0	37.0	37.0	35.0	37.0
6	36.2770163355911	37.0	37.0	37.0	35.0	37.0
7	36.23234062406753	37.0	37.0	37.0	35.0	37.0
8	36.21007309707008	37.0	37.0	37.0	35.0	37.0
9	37.88333350581344	39.0	38.0	39.0	35.0	39.0
10-11	37.851407480807275	39.0	38.0	39.0	35.0	39.0
12-13	37.82511474239234	39.0	38.0	39.0	35.0	39.0
14-15	39.22286348889314	41.0	39.0	41.0	36.0	41.0
16-17	39.20466049877798	40.0	39.0	41.0	36.0	41.0
18-19	39.23587465525539	40.5	39.0	41.0	36.0	41.0
20-21	39.15697990130239	40.0	39.0	41.0	36.0	41.0
22-23	39.15185280935866	40.0	39.0	41.0	36.0	41.0
24-25	39.05704351559823	40.0	39.0	41.0	35.0	41.0
26-27	38.91449097222075	40.0	38.0	41.0	35.0	41.0
28-29	38.78952862040502	40.0	38.0	41.0	35.0	41.0
30-31	38.668366006207	40.0	38.0	41.0	35.0	41.0
32-33	38.56516135805566	40.0	38.0	41.0	35.0	41.0
34-35	38.41606053188707	40.0	38.0	41.0	34.0	41.0
36-37	38.56506375588117	40.0	38.0	41.0	35.0	41.0
38-39	38.623748227273616	40.0	38.0	41.0	35.0	41.0
40-41	38.60903584016397	40.0	38.0	41.0	35.0	41.0
42-43	38.57062782789703	40.0	38.0	41.0	34.5	41.0
44-45	38.49654091991101	40.0	38.0	41.0	34.0	41.0
46-47	38.42451225874083	40.0	38.0	41.0	34.0	41.0
48-49	38.333416978647676	40.0	38.0	41.0	34.0	41.0
50-51	38.23774623793257	40.0	38.0	41.0	34.0	41.0
52-53	38.12721452812745	40.0	37.0	41.0	33.0	41.0
54-55	37.980113059184426	40.0	37.0	41.0	33.0	41.0
56-57	37.826813606520695	40.0	37.0	41.0	33.0	41.0
58-59	37.637841823118436	39.0	36.0	41.0	33.0	41.0
60-61	37.39929378741026	39.0	36.0	40.5	32.5	41.0
62-63	37.192972553158015	39.0	35.0	40.0	32.0	41.0
64-65	36.953256038656704	38.0	35.0	40.0	32.0	41.0
66-67	36.66558333907527	38.0	35.0	40.0	32.0	41.0
68-69	36.30483473062502	37.0	35.0	40.0	31.0	41.0
70-71	36.02649493699049	37.0	35.0	39.0	31.0	41.0
72-73	35.69510006521014	36.0	34.0	39.0	31.0	40.0
74-75	35.36386268934907	36.0	34.0	38.5	31.0	40.0
76-77	34.343654918905386	35.0	33.0	37.0	29.5	39.0
78-79	34.64244971751421	35.0	34.0	37.0	30.0	39.0
80-81	34.48252495953632	35.0	34.0	37.0	30.0	39.0
82-83	34.21322722276378	35.0	34.0	36.0	30.0	37.5
84-85	33.951561266427476	35.0	34.0	36.0	30.0	37.0
86-87	33.728703617828664	35.0	33.0	35.0	30.0	37.0
88-89	33.54870237539641	35.0	33.0	35.0	30.0	36.0
90-91	33.36261660129756	35.0	33.0	35.0	29.0	36.0
92-93	33.2286083038299	34.0	33.0	35.0	29.0	36.0
94-95	33.13993578928281	34.0	33.0	35.0	29.0	35.0
96-97	33.123540899392175	34.0	33.0	35.0	29.0	35.0
98-99	33.139055966736336	34.0	33.0	35.0	29.0	35.0
100-101	32.13642441653557	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	14.0
20	62.0
21	283.0
22	1342.0
23	4414.0
24	11660.0
25	24352.0
26	43596.0
27	69987.0
28	101884.0
29	138820.0
30	180067.0
31	229045.0
32	291232.0
33	381941.0
34	538586.0
35	784707.0
36	1364924.0
37	2748566.0
38	3338556.0
39	1295010.0
40	46491.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.56819949739296	21.34915665850836	15.889497168739014	24.193146675359664
2	13.657589642712495	21.58512823449619	41.31608107707179	23.441201045719527
3	16.932070433977202	26.373321121741633	31.367724142213298	25.326884302067864
4	11.942980830817783	18.938886756363804	38.71207711862294	30.40605529419547
5	12.998739170405175	37.93643935513137	35.547701961271315	13.51711951319214
6	29.495193841770195	38.34939123145623	18.616312823723604	13.539102103049967
7	26.155944440707373	33.07092209590929	22.63647057403105	18.136662889352284
8	24.580261031396557	36.58008165208347	20.90176912847526	17.937888188044717
9	25.3095845471621	17.02183770656649	21.11992196999881	36.548655776272604
10-11	23.94049098640256	27.12811103075874	28.63302414174659	20.298373841092115
12-13	24.96232603225033	24.577617773730246	28.821784065252675	21.63827212876675
14-15	22.03810020145401	25.96628738141739	26.762888600145374	25.23272381698322
16-17	21.433796382616844	29.22456507524414	27.339511674144024	22.002126867994992
18-19	21.442452998519517	28.094916501940965	29.429632378451746	21.032998121087772
20-21	22.320929903632372	27.111166018983752	29.384558928796277	21.183345148587605
22-23	21.93950240474545	27.163245613940518	29.29567102694113	21.601580954372906
24-25	21.76265291508703	27.330682209185003	29.355642080209815	21.55102279551815
26-27	21.7202193763829	27.50141685651228	29.247392317286792	21.530971449818033
28-29	21.446042628961802	27.47623301171683	29.467596596466635	21.610127762854738
30-31	21.858324967599668	27.286185760288944	29.213904781206413	21.64158449090498
32-33	21.782150833336374	27.394477608388957	29.25432012697996	21.56905143129471
34-35	21.84432475967969	27.612820723035743	29.17341063529032	21.36944388199425
36-37	21.696743292240093	27.471592192488657	29.178153880991438	21.65351063427981
38-39	21.59365796003504	27.546143957136156	29.245588077984504	21.614610004844312
40-41	21.845513039770744	27.43982879926233	29.106123438386515	21.608534722580412
42-43	21.84410091224618	27.48562218285967	28.92060507951697	21.749671825377177
44-45	21.860428099743416	27.715286728486593	28.647482159534977	21.776803012235018
46-47	22.056566671834627	27.593136247098204	28.247723405769023	22.10257367529815
48-49	21.970894848368978	27.751477990653733	28.215640908291817	22.06198625268547
50-51	21.904311975579933	27.874166628752572	28.153677114966115	22.06784428070138
52-53	22.10190422994816	27.840281579723896	28.073928520259884	21.983885670068055
54-55	22.062450864699954	27.78766570304125	28.020522354159503	22.129361078099294
56-57	22.160669127239697	27.925074684815677	27.843253333096037	22.071002854848594
58-59	21.95595818220352	28.113549567492647	28.019603839355167	21.91088841094867
60-61	22.182670478492756	28.16863282408975	27.79961338825335	21.849083309164147
62-63	22.062543084094244	28.26014966765363	27.745866124232325	21.931441124019805
64-65	22.03812329149125	28.138993478623757	27.792721168222045	22.030162061662946
66-67	22.13799379565602	28.103209571431982	27.653212566713535	22.105584066198464
68-69	22.102650860727405	27.966250149008232	27.793383297916634	22.137715692347733
70-71	22.12449830377558	27.757476231762997	27.846065855298292	22.27195960916313
72-73	22.16203014404133	27.831740540335677	27.788115171377243	22.218114144245753
74-75	22.02484714975798	27.91662863915642	27.752171739663716	22.30635247142188
76-77	22.1330835306298	27.86518030154256	27.70469778249248	22.297038385335156
78-79	22.138508240293522	27.851540441265744	27.696215821786936	22.313735496653806
80-81	22.13623399171013	27.839822570498885	27.7105788773829	22.31336456040808
82-83	22.192162892604188	27.796715532489607	27.791482162080822	22.219639412825384
84-85	22.293295186329818	27.7886968917629	27.716828185869723	22.20117973603756
86-87	22.134481291189058	27.796496132263073	27.77157447605959	22.297448100488282
88-89	22.177261001915262	27.728342945331434	27.75313676708	22.341259285673303
90-91	22.222428732143285	27.7292395993705	27.817105242729813	22.231226425756397
92-93	22.25358449102142	27.65718938675071	27.7922512712421	22.29697485098577
94-95	22.097445556790362	27.721873614069835	27.850740377162335	22.329940451977464
96-97	22.065174536343314	27.774363147005555	27.911605000188512	22.248857316462622
98-99	22.30286388368537	28.325004490918314	27.96716568798589	21.404965937410427
100-101	22.798919806240228	31.03122894773131	25.144252028123976	21.02559921790449
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3177.0
1	2755.0
2	3328.0
3	4557.5
4	6006.5
5	8800.0
6	11202.0
7	14288.5
8	17388.0
9	18399.5
10	17128.0
11	15669.5
12	17468.0
13	24132.5
14	39858.5
15	60691.0
16	76842.0
17	84440.5
18	83700.5
19	77133.5
20	68139.0
21	59339.0
22	54099.0
23	56212.5
24	65638.0
25	81276.0
26	101815.5
27	124395.5
28	148646.0
29	174096.0
30	197830.0
31	221843.0
32	248562.0
33	275772.0
34	301311.0
35	325694.5
36	352783.0
37	375649.0
38	392287.0
39	406234.0
40	420589.5
41	434327.5
42	442777.5
43	451110.0
44	459382.0
45	467610.5
46	474195.0
47	478289.0
48	484027.0
49	477570.0
50	461058.5
51	444238.0
52	421586.5
53	397265.0
54	371805.5
55	341856.0
56	310233.0
57	278570.5
58	246227.0
59	214231.0
60	181353.5
61	148569.0
62	116876.5
63	89257.0
64	66944.0
65	49908.0
66	37750.0
67	28834.0
68	21985.0
69	15816.5
70	10955.5
71	7659.5
72	5340.0
73	3728.5
74	2504.0
75	1659.0
76	1071.0
77	654.5
78	410.0
79	265.0
80	184.5
81	138.5
82	104.0
83	74.5
84	48.5
85	32.5
86	25.5
87	20.0
88	14.0
89	8.5
90	6.0
91	5.0
92	3.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.007278660588467635
3	0.0
4	8.624005436573029E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.459764702635669E-4
12-13	0.0
14-15	7.71848486573286E-4
16-17	7.416644675452803E-4
18-19	8.624005436573029E-6
20-21	4.872690608768523E-4
22-23	0.0012031668386174638
24-25	0.004727302574840079
26-27	0.017038644716255436
28-29	0.3178630376036912
30-31	0.3051990919385042
32-33	0.3135545219641822
34-35	0.18075460110214442
36-37	0.043670997363018986
38-39	0.033550527935146145
40-41	0.03084119197115341
42-43	0.027126111359261684
44-45	0.014843648538462945
46-47	0.00784819412017081
48-49	0.0053369067879226436
50-51	0.0021625456350963562
52-53	0.001628802168733938
54-55	0.0011943130622623745
56-57	4.402503263355544E-4
58-59	0.002908861222386596
60-61	0.002276376055038392
62-63	2.929697818479765E-4
64-65	1.2598400167926983E-4
66-67	0.007928057866600736
68-69	0.007158804481629138
70-71	0.009485674260448159
72-73	0.010033432849600253
74-75	0.007894854376053686
76-77	0.004987381308928209
78-79	3.164903564610131E-4
80-81	4.772407054225977E-4
82-83	5.67342914677034E-4
84-85	0.0
86-87	0.0016050243334702666
88-89	0.0012407737355211723
90-91	0.0010850927509460618
92-93	0.011864158602737544
94-95	1.1474699721451664E-4
96-97	4.676239669675106E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	186.0
20-21	664.0
22-23	1440.0
24-25	3733.0
26-27	5385.0
28-29	8431.0
30-31	18503.0
32-33	25353.0
34-35	37460.0
36-37	51683.0
38-39	61713.0
40-41	67936.0
42-43	84499.0
44-45	103706.0
46-47	102817.0
48-49	89025.0
50-51	78290.0
52-53	75573.0
54-55	81379.0
56-57	87388.0
58-59	88729.0
60-61	88937.0
62-63	90476.0
64-65	98002.0
66-67	105735.0
68-69	99500.0
70-71	94211.0
72-73	92451.0
74-75	94150.0
76-77	95905.0
78-79	102057.0
80-81	104744.0
82-83	102153.0
84-85	104969.0
86-87	112004.0
88-89	119284.0
90-91	129811.0
92-93	135280.0
94-95	151904.0
96-97	230384.0
98-99	996285.0
100-101	7373405.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.16150711656343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.86872795834458	74.79326080506156
2	7.72241359309787	12.998599331451052
3	1.9523655252152092	4.929420751335988
4	0.6954480437885373	2.3411982194603635
5	0.31588071925680455	1.3292498700858364
6	0.15111015822021245	0.7630595193861262
7	0.08678507751142153	0.5112774043012308
8	0.053206210148020266	0.3582331867214381
9	0.03610302485108185	0.2734636484640412
>10	0.11493182722546523	1.4491767404125362
>50	0.0023985130317982144	0.13226525308322643
>100	5.788359962030109E-4	0.08422397697310866
>500	3.367986945801175E-5	0.01779964779004045
>1k	1.6833443325717014E-5	0.018771645473360195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7248010873146054E-5	0.0	0.0	8.624005436573027E-6
3	0.0	1.7248010873146054E-5	0.0	0.0	8.624005436573027E-6
4	0.0	5.174403261943816E-5	0.0	1.7248010873146054E-5	8.624005436573027E-6
5	0.0	6.036803805601119E-5	0.0	1.7248010873146054E-5	8.624005436573027E-6
6	2.587201630971908E-5	6.036803805601119E-5	0.0	1.7248010873146054E-5	8.624005436573027E-6
7	2.587201630971908E-5	6.036803805601119E-5	0.0	3.449602174629211E-5	8.624005436573027E-6
8	2.587201630971908E-5	6.899204349258421E-5	0.0	3.449602174629211E-5	8.624005436573027E-6
9	2.587201630971908E-5	1.1211207067544935E-4	0.0	5.174403261943816E-5	8.624005436573027E-6
10-11	3.0184019028005592E-5	1.1211207067544935E-4	0.0	8.624005436573028E-5	8.624005436573027E-6
12-13	3.449602174629211E-5	1.1211207067544935E-4	0.0	9.48640598023033E-5	8.624005436573027E-6
14-15	3.880802446457862E-5	1.6385610329488752E-4	0.0	1.1211207067544935E-4	1.293600815485954E-5
16-17	6.899204349258421E-5	1.854161168863201E-4	0.0	1.552320978583145E-4	2.587201630971908E-5
18-19	6.899204349258421E-5	2.0697613047775264E-4	0.0	1.8110411416803358E-4	4.312002718286514E-5
20-21	6.899204349258421E-5	2.242241413508987E-4	0.0	2.0697613047775264E-4	4.743202990115165E-5
22-23	6.899204349258421E-5	2.4147215222404477E-4	0.0	2.457841549423313E-4	6.46800407742977E-5
24-25	7.330404621087073E-5	2.500961576606178E-4	0.0	2.8459217940690986E-4	8.624005436573028E-5
26-27	7.761604892915725E-5	2.500961576606178E-4	0.0	3.837682419274997E-4	8.624005436573028E-5
28-29	7.761604892915725E-5	2.5872016309719085E-4	0.0	6.123043859966848E-4	8.624005436573028E-5
30-31	8.192805164744376E-5	3.147761984349155E-4	0.0	0.0010262566469521903	9.48640598023033E-5
32-33	9.055205708401679E-5	3.19088201153202E-4	0.0	0.0018024171362437625	1.0348806523887632E-4
34-35	9.48640598023033E-5	3.449602174629211E-4	0.0	0.003031337910955419	1.0348806523887632E-4
36-37	9.48640598023033E-5	3.7083223377264016E-4	0.0	0.005045043180395221	1.0348806523887632E-4
38-39	9.48640598023033E-5	3.794562392092132E-4	0.0	0.008024637058731202	1.0348806523887632E-4
40-41	9.48640598023033E-5	3.880802446457862E-4	0.0	0.011284511113755806	1.0348806523887632E-4
42-43	9.48640598023033E-5	4.096402582372188E-4	0.0	0.014863473369933612	1.0348806523887632E-4
44-45	1.2073607611202238E-4	4.6138429085665697E-4	0.0	0.018817579862602345	1.0348806523887632E-4
46-47	1.2073607611202238E-4	4.915683098846626E-4	0.0	0.023125270578170574	1.0348806523887632E-4
48-49	1.2073607611202238E-4	4.915683098846626E-4	0.0	0.027342409236654784	1.3798408698516843E-4
50-51	1.250480788303089E-4	5.605603533772468E-4	0.0	0.03167597196853273	1.4229608970345493E-4
52-53	1.3798408698516843E-4	5.778083642503928E-4	0.0	0.0358413665943975	1.7248010873146055E-4
54-55	1.3798408698516843E-4	5.821203669686794E-4	0.0	0.040248233372486314	1.9835212504117961E-4
56-57	1.3798408698516843E-4	5.950563751235388E-4	0.0	0.045254468528416955	2.0266412775946612E-4
58-59	1.3798408698516843E-4	6.03680380560112E-4	0.0	0.04975188736358979	2.1128813319603917E-4
60-61	1.6816810601317405E-4	6.252403941515445E-4	0.0	0.05443903431836723	2.1560013591432567E-4
62-63	1.9835212504117961E-4	6.468004077429771E-4	0.0	0.05907443724052523	2.1560013591432567E-4
64-65	1.9835212504117961E-4	7.330404621087073E-4	0.0	0.0635632320702615	2.1560013591432567E-4
66-67	2.1128813319603917E-4	7.416644675452803E-4	0.0	0.06786229878039315	2.1560013591432567E-4
68-69	2.1560013591432567E-4	7.416644675452803E-4	0.0	0.07257531775148031	2.1560013591432567E-4
70-71	2.242241413508987E-4	7.459764702635668E-4	0.0	0.07705117657306171	2.3284814678747174E-4
72-73	2.587201630971908E-4	7.502884729818533E-4	0.0	0.08083711495971727	2.500961576606178E-4
74-75	2.587201630971908E-4	7.847844947281456E-4	0.0	0.08388570088154584	2.500961576606178E-4
76-77	2.7596817397033686E-4	8.494645355024431E-4	0.0	0.0865807025804749	2.500961576606178E-4
78-79	2.845921794069099E-4	8.710245490938757E-4	0.0	0.08912909618698224	2.544081603789043E-4
80-81	2.845921794069099E-4	8.710245490938757E-4	0.0	0.09030627292907445	2.6734416853376386E-4
82-83	2.845921794069099E-4	8.753365518121623E-4	0.0	0.0907116011845934	2.802801766886234E-4
84-85	2.975281875617694E-4	8.839605572487353E-4	0.0	0.09101344137487344	2.845921794069099E-4
86-87	3.061521929983425E-4	9.055205708401678E-4	0.0	0.09108243341836603	2.845921794069099E-4
88-89	3.10464195716629E-4	9.529526007413195E-4	0.0	0.09112986544826718	2.845921794069099E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17290	0.0	53.18083	1
GTATCAA	31115	0.0	37.71275	1
ATCAACG	39665	0.0	29.180374	3
TCAACGC	39720	0.0	29.12928	4
CAACGCA	40715	0.0	28.428207	5
TATCAAC	41105	0.0	28.327677	2
AACGCAG	41950	0.0	27.622013	6
CTTATAC	8785	0.0	27.193764	1
ACGCAGA	48525	0.0	23.888163	7
CGCAGAG	48980	0.0	23.675026	8
TGGTATC	8060	0.0	22.76298	2
GTGGTAT	8440	0.0	22.501741	1
TATACAC	13675	0.0	20.955557	3
GCAGAGT	55820	0.0	20.635866	9
GAGTACT	32920	0.0	20.223087	12-13
GTACATG	38720	0.0	19.452835	1
AGAGTAC	51725	0.0	18.909704	10-11
TACATGG	38685	0.0	18.848448	2
CAGAGTA	55090	0.0	18.59697	10-11
GTACTTT	36035	0.0	18.475288	14-15
>>END_MODULE