FastQCFastQC Report
Fri 27 May 2016
SRR2139951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027388
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151300.054.198591
CTTATAC101700.042.137931
GTATCAA279500.038.117821
TATACAC148500.031.4939143
TCAACGC345600.030.2450074
ATCAACG346950.030.0535133
TTATACA148900.029.8104952
CAACGCA351650.029.7007265
TATCAAC358100.029.453882
AACGCAG362900.028.7799956
ACGCAGA415050.025.122817
CGCAGAG419300.024.837698
TACACAT199650.023.4682455
ACACATC185300.023.1019086
ACATCTC191300.023.0453138
GTGGTAT68700.022.7565021
TGGTATC66550.022.0834672
GCAGAGT478850.021.5889669
GAGTACT273450.020.6863512-13
CACATCT209500.020.3723417
Produced by FastQC (version 0.11.5)