##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10027388
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65604223153627	34.0	31.0	34.0	31.0	34.0
2	32.814216124877184	34.0	31.0	34.0	31.0	34.0
3	32.85946040982956	34.0	31.0	34.0	31.0	34.0
4	36.32139994981744	37.0	37.0	37.0	35.0	37.0
5	36.25727856546491	37.0	37.0	37.0	35.0	37.0
6	36.290791879201244	37.0	37.0	37.0	35.0	37.0
7	36.24493447346408	37.0	37.0	37.0	35.0	37.0
8	36.22324517611167	37.0	37.0	37.0	35.0	37.0
9	37.90152759621947	39.0	38.0	39.0	35.0	39.0
10-11	37.874557362296144	39.0	38.0	39.0	35.0	39.0
12-13	37.84983227935331	39.0	38.0	39.0	35.0	39.0
14-15	39.2647772281276	41.0	39.0	41.0	36.0	41.0
16-17	39.24664952627743	41.0	39.0	41.0	36.0	41.0
18-19	39.27788707288478	41.0	39.0	41.0	36.0	41.0
20-21	39.198215902482644	41.0	39.0	41.0	36.0	41.0
22-23	39.20071516475292	40.5	39.0	41.0	36.0	41.0
24-25	39.10686332799507	40.0	39.0	41.0	35.5	41.0
26-27	38.96169012716797	40.0	39.0	41.0	35.0	41.0
28-29	38.82808498799007	40.0	38.0	41.0	35.0	41.0
30-31	38.702431572426455	40.0	38.0	41.0	35.0	41.0
32-33	38.60692842657434	40.0	38.0	41.0	35.0	41.0
34-35	38.455970958553706	40.0	38.0	41.0	34.5	41.0
36-37	38.592936656952034	40.0	38.0	41.0	35.0	41.0
38-39	38.641427443790135	40.0	38.0	41.0	35.0	41.0
40-41	38.635438095469596	40.0	38.0	41.0	35.0	41.0
42-43	38.60630092367981	40.0	38.0	41.0	34.5	41.0
44-45	38.533295578915585	40.0	38.0	41.0	34.0	41.0
46-47	38.46526392141804	40.0	38.0	41.0	34.0	41.0
48-49	38.38010586626871	40.0	38.0	41.0	34.0	41.0
50-51	38.27691247175338	40.0	38.0	41.0	34.0	41.0
52-53	38.16264278764257	40.0	37.0	41.0	33.5	41.0
54-55	38.02453544434688	40.0	37.0	41.0	33.0	41.0
56-57	37.86703945075666	40.0	37.0	41.0	33.0	41.0
58-59	37.66773231021834	39.0	36.0	41.0	33.0	41.0
60-61	37.43813479267032	39.0	36.0	41.0	33.0	41.0
62-63	37.23811108107661	39.0	35.0	40.0	32.0	41.0
64-65	37.00571668073991	38.5	35.0	40.0	32.0	41.0
66-67	36.72734042046784	38.0	35.0	40.0	32.0	41.0
68-69	36.373762762294646	37.0	35.0	40.0	31.0	41.0
70-71	36.10077000551104	37.0	35.0	39.0	31.0	41.0
72-73	35.774885796810935	36.0	34.5	39.0	31.0	40.0
74-75	35.44397212401715	36.0	34.0	38.5	31.0	40.0
76-77	34.42974629415602	35.0	33.5	37.0	29.5	39.0
78-79	34.70992817324321	35.0	34.0	37.0	30.0	39.0
80-81	34.545723083695364	35.0	34.0	37.0	30.0	39.0
82-83	34.27619356590084	35.0	34.0	36.0	30.0	38.0
84-85	34.017129220097644	35.0	34.0	36.0	30.0	37.0
86-87	33.79634635956105	35.0	33.5	35.0	30.0	37.0
88-89	33.616492930320234	35.0	33.0	35.0	30.0	36.5
90-91	33.435267903589995	35.0	33.0	35.0	30.0	36.0
92-93	33.304243051720675	35.0	33.0	35.0	29.0	36.0
94-95	33.21750167402705	34.0	33.0	35.0	29.0	35.5
96-97	33.20103683203918	34.0	33.0	35.0	29.0	35.0
98-99	33.2151981031249	34.0	33.0	35.0	30.0	35.0
100-101	32.22452915865193	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	4.0
19	8.0
20	37.0
21	250.0
22	1290.0
23	4329.0
24	10879.0
25	22546.0
26	39721.0
27	62622.0
28	89406.0
29	119701.0
30	154258.0
31	193417.0
32	244266.0
33	318285.0
34	445066.0
35	648464.0
36	1117960.0
37	2276461.0
38	2959609.0
39	1266990.0
40	51818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.715160789146765	21.41672601246965	15.840577410645466	24.02753578773812
2	13.677586614608689	21.437712193660946	41.700838566708086	23.18386262502228
3	16.904034919565785	26.583027063780424	31.4922621416726	25.02067587498119
4	11.90973400246086	19.080326617525245	38.62338599511378	30.38655338490011
5	12.89764721357618	38.036090558786654	35.66162351168854	13.40463871594863
6	29.23326884660979	38.40442180998898	18.696854923421228	13.66545441998
7	26.024885645682172	33.16826208063976	22.606607284629586	18.200244989048493
8	24.487603351939708	36.72821875447524	21.02958417486189	17.75459371872316
9	25.178810274420414	17.21932969981814	21.092691336966315	36.50916868879513
10-11	23.888001201519373	27.138845097346202	28.690947237662286	20.282206463472136
12-13	24.932574664508845	24.52771848461434	28.81444300350201	21.72526384737481
14-15	22.066367922656056	26.025124973646978	26.51869184509431	25.389815258602656
16-17	21.531964816871053	29.39763681291864	27.101466734933588	21.968931635276714
18-19	21.550075807386314	28.172853007468447	29.12523970601713	21.15183147912811
20-21	22.47790316571904	27.12835474132319	29.056711896115893	21.337030196841873
22-23	22.08671908326703	27.34464647818673	28.960411074450544	21.608223364095696
24-25	21.987010207263346	27.669065608743836	28.952327280086127	21.39159690390669
26-27	21.98051321796904	27.81637200105701	28.86169235617787	21.34142242479608
28-29	21.604982237420813	27.72916271600419	29.068791664775855	21.597063381799135
30-31	22.294525745034637	27.437075438784802	28.95557809459777	21.312820721582796
32-33	21.56209629825658	27.895425009961617	28.959353984777962	21.583124707003837
34-35	21.89080637050872	27.81300449341843	29.0676443488789	21.228544787193947
36-37	21.690007553223598	27.76859234864905	28.97399625243906	21.567403845688286
38-39	21.627417473156537	27.447859923343003	29.194305500633515	21.730417102866937
40-41	21.83032588192618	27.354531872771627	29.166588235366138	21.64855400993606
42-43	21.957466821780216	27.362658339302104	28.98053002469625	21.699344814221433
44-45	21.849505026790876	27.69126502452054	28.664271975243416	21.794957973445168
46-47	22.170746031843162	27.468740191633483	28.17014382004066	22.19036995648269
48-49	22.02274728540443	27.557239952949615	28.228256041452326	22.191756720193627
50-51	21.884845752779533	27.746326391060933	28.246259672233244	22.12256818392629
52-53	22.043499719902353	27.725235287657146	28.32754851167308	21.903716480767425
54-55	22.00336820123045	27.734170978925928	28.0887901877079	22.17367063213572
56-57	22.214105766796127	27.98393465467919	27.761283045782807	22.04067653274188
58-59	21.84717792842259	28.249524336543928	28.12961991642598	21.7736778186075
60-61	22.184265791695452	28.47916276508094	27.607324871107924	21.72924657211568
62-63	21.923435570028126	28.608339888648228	27.60704807681612	21.861176464507533
64-65	21.98285541003661	28.46072563960637	27.646822332468417	21.909596617888603
66-67	22.12042499993967	28.264655208968108	27.573589614503806	22.041330176588414
68-69	22.10603180716973	28.02890537487952	27.751088056774325	22.113974761176422
70-71	22.108545064370873	27.831667939916404	27.811636078205847	22.248150917506877
72-73	22.153676943530858	27.87026650789065	27.773245956655764	22.202810591922727
74-75	22.038771891862336	27.974247763410293	27.73330469788614	22.253675646841238
76-77	22.15955880094816	27.898261137508477	27.704500287751944	22.237679773791417
78-79	22.140485589671492	27.908994100698592	27.692954722639783	22.257565586990133
80-81	22.140633357631057	27.912827619137424	27.698686517450614	22.247852505780898
82-83	22.17525485542926	27.851892771102825	27.798004622831503	22.174847750636413
84-85	22.275953968858126	27.833822580620637	27.74797412598151	22.142249324539723
86-87	22.11867826855946	27.82069020173705	27.798735637681983	22.261895892021506
88-89	22.172895830437533	27.798369171972205	27.780773532625002	22.247961464965258
90-91	22.243353345728774	27.753221528443955	27.830523983653194	22.172901142174076
92-93	22.24101952391671	27.716370584088533	27.828084466019547	22.21452542597521
94-95	22.060977211677187	27.75189776972855	27.880115765873175	22.307009252721084
96-97	22.064695281243562	27.740699512318418	27.95915039185916	22.235454814578855
98-99	22.252748696517628	28.386496696524826	27.972621920820984	21.38813268613656
100-101	22.791639233897516	31.07942153604367	25.20579818793724	20.92314104212157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2325.0
1	2047.5
2	2580.5
3	3593.0
4	4842.5
5	7260.5
6	9339.5
7	11783.5
8	14364.0
9	15257.5
10	14245.0
11	13104.5
12	14339.0
13	19232.5
14	31536.5
15	48553.5
16	62016.0
17	69059.0
18	69311.0
19	64219.0
20	56960.5
21	49654.0
22	45364.0
23	47008.5
24	54601.5
25	67207.5
26	84023.0
27	102917.0
28	123670.0
29	146679.5
30	169520.5
31	192119.0
32	216724.0
33	243454.5
34	267975.5
35	289900.5
36	313946.0
37	334554.0
38	348048.5
39	359667.5
40	372860.0
41	384417.0
42	390328.5
43	396760.0
44	405867.5
45	418401.5
46	433818.5
47	442378.0
48	434355.5
49	420492.0
50	409632.0
51	393020.0
52	370140.0
53	345095.0
54	321043.5
55	294895.0
56	266966.5
57	238936.5
58	210842.0
59	182723.0
60	154021.0
61	126470.5
62	99685.5
63	76294.0
64	57652.0
65	43724.0
66	33616.0
67	26079.0
68	20124.0
69	14365.5
70	10004.5
71	7181.5
72	5118.5
73	3669.5
74	2532.5
75	1702.5
76	1154.5
77	751.5
78	482.0
79	306.0
80	194.5
81	123.0
82	83.5
83	60.0
84	47.5
85	39.0
86	28.0
87	19.0
88	11.0
89	6.0
90	3.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.972686805377433E-6
2	0.008726100954705253
3	9.972686805377433E-6
4	1.9945373610754865E-5
5	9.972686805377433E-6
6	9.972686805377433E-6
7	9.972686805377433E-6
8	0.0
9	0.0
10-11	8.377056916517043E-4
12-13	0.0
14-15	7.379788235979299E-4
16-17	8.177603180409494E-4
18-19	9.972686805377433E-6
20-21	4.886753233671824E-4
22-23	0.0012915976089984957
24-25	0.00426965824727636
26-27	0.016461267570494542
28-29	0.3220846352867803
30-31	0.3097966480328543
32-33	0.31858746703684815
34-35	0.18319049114664654
36-37	0.044655833988786336
38-39	0.034390673796678685
40-41	0.031005818027567945
42-43	0.026594970459816257
44-45	0.014058143965646556
46-47	0.0068354787601749
48-49	0.005364588184431162
50-51	0.0017731026084895193
52-53	0.0016256720114970957
54-55	0.0011516286759865686
56-57	4.636394573760156E-4
58-59	0.0022138760685394003
60-61	0.0021135556730865553
62-63	2.5842911502859685E-4
64-65	1.4761883159808344E-4
66-67	0.007669874359414679
68-69	0.006895822724561653
70-71	0.008951230779538768
72-73	0.009468432690633535
74-75	0.00773984527503729
76-77	0.004912227400150044
78-79	3.979173617466736E-4
80-81	5.324708461376577E-4
82-83	7.390463158447209E-4
84-85	0.0
86-87	0.0017568472158459925
88-89	0.0013769745945589238
90-91	0.001284405688033403
92-93	0.011727712732284267
94-95	1.6988787671957112E-4
96-97	4.154899932351304E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	635.0
22-23	1275.0
24-25	3775.0
26-27	5020.0
28-29	8195.0
30-31	17981.0
32-33	24419.0
34-35	36010.0
36-37	50581.0
38-39	59408.0
40-41	63245.0
42-43	77592.0
44-45	94311.0
46-47	94112.0
48-49	82055.0
50-51	72159.0
52-53	69884.0
54-55	77842.0
56-57	87579.0
58-59	89058.0
60-61	90243.0
62-63	91701.0
64-65	98754.0
66-67	107484.0
68-69	94636.0
70-71	85996.0
72-73	82459.0
74-75	84968.0
76-77	85777.0
78-79	91123.0
80-81	92923.0
82-83	90868.0
84-85	93113.0
86-87	98310.0
88-89	104879.0
90-91	112673.0
92-93	116375.0
94-95	130448.0
96-97	193368.0
98-99	835765.0
100-101	6230220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.94478135790716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39991747355833	75.94056443206357
2	7.417705290146006	12.601907080976874
3	1.8158435918714113	4.6273931067502145
4	0.6525655836533366	2.2172816330051104
5	0.28631679298925783	1.2160558689784835
6	0.1467399060189488	0.747887353995567
7	0.09013210333169247	0.5359376267587239
8	0.05180993033774247	0.3520786563766348
9	0.035056656023794826	0.26800919829725495
>10	0.10164244811920799	1.303815850581653
>50	0.0017297491320613393	0.09752960795836808
>100	5.029039985821139E-4	0.07077933728185835
>500	3.757081960079238E-5	0.020760246975667297
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.972686805377433E-6	0.0	9.972686805377433E-6	0.0
3	0.0	9.972686805377433E-6	0.0	9.972686805377433E-6	0.0
4	0.0	4.9863434026887164E-5	0.0	4.9863434026887164E-5	0.0
5	0.0	4.9863434026887164E-5	0.0	5.983612083226459E-5	0.0
6	7.978149444301946E-5	4.9863434026887164E-5	0.0	7.978149444301946E-5	0.0
7	7.978149444301946E-5	4.9863434026887164E-5	0.0	8.975418124839689E-5	0.0
8	7.978149444301946E-5	5.983612083226459E-5	0.0	8.975418124839689E-5	0.0
9	7.978149444301946E-5	1.6953567569141635E-4	0.0	1.3961761527528407E-4	9.972686805377433E-6
10-11	7.978149444301946E-5	1.6953567569141635E-4	0.0	2.2937179652368094E-4	9.972686805377433E-6
12-13	7.978149444301946E-5	1.6953567569141635E-4	0.0	2.8920791735594553E-4	9.972686805377433E-6
14-15	7.978149444301946E-5	2.0942642291292608E-4	0.0	3.0416694756401166E-4	2.99180604161323E-5
16-17	1.296449284699066E-4	2.1441276631561478E-4	0.0	3.390713513828327E-4	5.983612083226459E-5
18-19	1.296449284699066E-4	2.493171701344358E-4	0.0	4.03893815617786E-4	5.983612083226459E-5
20-21	1.296449284699066E-4	2.7923523055056813E-4	0.0	4.637299364500506E-4	6.482246423495332E-5
22-23	1.3463127187259534E-4	3.091532909667004E-4	0.0	4.8367531006080546E-4	1.0471321145646303E-4
24-25	1.3961761527528407E-4	3.590167249935876E-4	0.0	5.036206836715603E-4	1.296449284699066E-4
26-27	1.3961761527528407E-4	3.7397575520165373E-4	0.0	5.484977742957588E-4	1.3961761527528407E-4
28-29	1.3961761527528407E-4	3.8893478540971986E-4	0.0	8.726100954705254E-4	1.4460395867797277E-4
30-31	1.3961761527528407E-4	4.637299364500506E-4	0.0	0.0017103157871222298	1.495903020806615E-4
32-33	1.4460395867797277E-4	4.7868896665811673E-4	0.0	0.003470495008271347	1.5956298888603892E-4
34-35	1.5956298888603892E-4	5.484977742957588E-4	0.0	0.005639554388440938	1.6953567569141635E-4
36-37	1.5956298888603892E-4	6.083338951280233E-4	0.0	0.009334434849833276	1.7950836249679378E-4
38-39	1.6454933228872762E-4	6.282792687387782E-4	0.0	0.015158483944173698	1.7950836249679378E-4
40-41	1.6953567569141635E-4	6.581973291549105E-4	0.0	0.02135650879371577	1.894810493021712E-4
42-43	1.6953567569141635E-4	6.881153895710427E-4	0.0	0.02725535303909652	1.9945373610754866E-4
44-45	1.6953567569141635E-4	8.177603180409495E-4	0.0	0.034410755821954825	2.193991097183035E-4
46-47	1.6953567569141635E-4	9.075144992893463E-4	0.0	0.04244874138708904	2.3934448332905837E-4
48-49	1.6953567569141635E-4	9.075144992893463E-4	0.0	0.05053160404284745	2.3934448332905837E-4
50-51	1.8449470589948248E-4	0.001037159427759253	0.0	0.05812081870173967	2.3934448332905837E-4
52-53	1.894810493021712E-4	0.0010670774881753854	0.0	0.06568011530021577	2.6427620034250195E-4
54-55	1.894810493021712E-4	0.0010820365183834514	0.0	0.07343886563479941	3.0416694756401166E-4
56-57	1.894810493021712E-4	0.001106968235396895	0.0	0.07926790107254253	3.1912597777207784E-4
58-59	1.894810493021712E-4	0.0011169409222022725	0.0	0.08446367089814416	3.2909866457745524E-4
60-61	2.0444007951023736E-4	0.0011468589826184048	0.0	0.08954475482548396	3.390713513828327E-4
62-63	2.3934448332905837E-4	0.0011668043562291595	0.0	0.09460589337921302	3.4904403818821015E-4
64-65	2.3934448332905837E-4	0.001316394658309821	0.0	0.0997368407405797	3.4904403818821015E-4
66-67	2.3934448332905837E-4	0.0013612717489340195	0.0	0.10491266519257059	3.4904403818821015E-4
68-69	2.3934448332905837E-4	0.0013911898093501517	0.0	0.11040262927893085	3.4904403818821015E-4
70-71	2.3934448332905837E-4	0.0014211078697662842	0.0	0.11524935506634429	3.640030683962763E-4
72-73	2.493171701344358E-4	0.0014360668999743502	0.0	0.11945284255481088	4.487709062419845E-4
74-75	2.493171701344358E-4	0.0014659849603904825	0.0	0.12250448471725638	4.587435930473619E-4
76-77	2.493171701344358E-4	0.001545766454833502	0.0	0.1251971101547083	4.637299364500506E-4
78-79	2.493171701344358E-4	0.0015856572020550117	0.0	0.1277501179768849	4.6871627985273933E-4
80-81	2.493171701344358E-4	0.0015906435454577004	0.0	0.1289069496463087	4.836753100608055E-4
82-83	2.543035135371245E-4	0.0015956298888603893	0.0	0.12940059764317485	4.986343402688716E-4
84-85	2.592898569398132E-4	0.0015956298888603893	0.0	0.129654901156712	4.986343402688716E-4
86-87	2.592898569398132E-4	0.0015956298888603893	0.0	0.12981945048900073	5.086070270742491E-4
88-89	2.692625437451907E-4	0.0016654386964980311	0.0	0.12985435489281955	5.086070270742491E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15130	0.0	54.19859	1
CTTATAC	10170	0.0	42.13793	1
GTATCAA	27950	0.0	38.11782	1
TATACAC	14850	0.0	31.493914	3
TCAACGC	34560	0.0	30.245007	4
ATCAACG	34695	0.0	30.053513	3
TTATACA	14890	0.0	29.810495	2
CAACGCA	35165	0.0	29.700726	5
TATCAAC	35810	0.0	29.45388	2
AACGCAG	36290	0.0	28.779995	6
ACGCAGA	41505	0.0	25.12281	7
CGCAGAG	41930	0.0	24.83769	8
TACACAT	19965	0.0	23.468245	5
ACACATC	18530	0.0	23.101908	6
ACATCTC	19130	0.0	23.045313	8
GTGGTAT	6870	0.0	22.756502	1
TGGTATC	6655	0.0	22.083467	2
GCAGAGT	47885	0.0	21.588966	9
GAGTACT	27345	0.0	20.68635	12-13
CACATCT	20950	0.0	20.372341	7
>>END_MODULE