FastQCFastQC Report
Fri 27 May 2016
SRR2139950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9643826
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177400.052.9294931
GTATCAA324350.037.628811
CTTATAC80050.031.5191651
ATCAACG408650.029.4500393
TCAACGC412150.029.2000984
TATCAAC417900.029.0837632
CAACGCA422100.028.4819855
AACGCAG435700.027.6706716
GTGGTAT77750.025.6454491
TGGTATC75000.025.5133192
ACGCAGA501100.024.0592947
CGCAGAG505400.023.8378438
TATACAC134700.022.5640663
GCAGAGT573100.020.9112019
TTATACA133600.020.533282
GAGTACT313450.020.0484812-13
GTACATG433200.019.3884471
ACATCTC134250.019.2030538
TACATGG431100.019.1293722
CAGAGTA566250.018.7458710-11
Produced by FastQC (version 0.11.5)