##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9643826
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.675498707670585	34.0	31.0	34.0	31.0	34.0
2	32.826883852943844	34.0	31.0	34.0	31.0	34.0
3	32.87201894766662	34.0	31.0	34.0	31.0	34.0
4	36.32611382660782	37.0	37.0	37.0	35.0	37.0
5	36.26862512865744	37.0	37.0	37.0	35.0	37.0
6	36.30811111689489	37.0	37.0	37.0	35.0	37.0
7	36.265642080228325	37.0	37.0	37.0	35.0	37.0
8	36.246890912382696	37.0	37.0	37.0	35.0	37.0
9	37.925091141213045	39.0	38.0	39.0	35.0	39.0
10-11	37.9060661712478	39.0	38.0	39.0	35.0	39.0
12-13	37.87730212054842	39.0	38.0	39.0	35.0	39.0
14-15	39.28445712313764	41.0	39.0	41.0	36.0	41.0
16-17	39.274681905293605	41.0	39.0	41.0	36.0	41.0
18-19	39.320553533421275	41.0	39.0	41.0	36.0	41.0
20-21	39.2514255994594	41.0	39.0	41.0	36.0	41.0
22-23	39.250718851914556	41.0	39.0	41.0	36.0	41.0
24-25	39.15617020004694	40.5	39.0	41.0	36.0	41.0
26-27	39.015768753904034	40.0	39.0	41.0	35.0	41.0
28-29	38.88500119781271	40.0	39.0	41.0	35.0	41.0
30-31	38.75592338463842	40.0	38.0	41.0	35.0	41.0
32-33	38.652324342848374	40.0	38.0	41.0	35.0	41.0
34-35	38.50664453311866	40.0	38.0	41.0	34.5	41.0
36-37	38.63924659906493	40.0	38.0	41.0	35.0	41.0
38-39	38.69378472149869	40.0	38.0	41.0	35.0	41.0
40-41	38.68650206794733	40.0	38.0	41.0	35.0	41.0
42-43	38.660004052541865	40.0	38.0	41.0	35.0	41.0
44-45	38.59239064877029	40.0	38.0	41.0	34.5	41.0
46-47	38.529619014007096	40.0	38.0	41.0	34.0	41.0
48-49	38.450287797123146	40.0	38.0	41.0	34.0	41.0
50-51	38.360291172352234	40.0	38.0	41.0	34.0	41.0
52-53	38.26357870864605	40.0	38.0	41.0	34.0	41.0
54-55	38.13872435395974	40.0	37.0	41.0	34.0	41.0
56-57	37.997725919736396	40.0	37.0	41.0	33.0	41.0
58-59	37.81760309374006	40.0	36.5	41.0	33.0	41.0
60-61	37.600029348256456	39.0	36.0	41.0	33.0	41.0
62-63	37.41481907410046	39.0	36.0	40.5	33.0	41.0
64-65	37.19306177353435	39.0	35.0	40.0	32.5	41.0
66-67	36.92486593738379	38.0	35.0	40.0	32.0	41.0
68-69	36.5792581417399	37.5	35.0	40.0	32.0	41.0
70-71	36.3081620642225	37.0	35.0	39.5	31.5	41.0
72-73	35.983422418549935	36.5	35.0	39.0	31.0	40.5
74-75	35.64920353662569	36.0	34.5	39.0	31.0	40.0
76-77	34.61758696473492	35.0	33.5	37.0	29.5	39.0
78-79	34.89263356217674	35.0	34.0	37.0	31.0	39.0
80-81	34.71450777118777	35.0	34.0	37.0	31.0	39.0
82-83	34.42965531658743	35.0	34.0	36.5	30.5	38.5
84-85	34.1576886180812	35.0	34.0	36.0	30.0	37.0
86-87	33.92170582017773	35.0	34.0	35.5	30.0	37.0
88-89	33.7297089132233	35.0	33.5	35.0	30.0	36.5
90-91	33.537415301754294	35.0	33.0	35.0	30.0	36.0
92-93	33.39568726049279	35.0	33.0	35.0	30.0	36.0
94-95	33.30013972581182	34.5	33.0	35.0	30.0	36.0
96-97	33.2763217490978	34.0	33.0	35.0	30.0	35.0
98-99	33.28394073235302	34.0	33.0	35.0	30.0	35.0
100-101	32.28816936138194	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	4.0
20	44.0
21	266.0
22	1119.0
23	3816.0
24	9729.0
25	19904.0
26	35256.0
27	55567.0
28	80345.0
29	108298.0
30	139668.0
31	176999.0
32	224268.0
33	293114.0
34	414545.0
35	597416.0
36	1013833.0
37	2132137.0
38	2934156.0
39	1347752.0
40	55587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.30911638370837	21.416224518406807	15.6862257129537	23.588433384931122
2	13.616118279418243	21.82680733011831	41.908163214513564	22.648911175949884
3	17.057007981791457	27.119083973423407	31.37083055737739	24.453077487407747
4	11.640364162673263	19.250282719859406	39.22404416008508	29.88530895738225
5	12.554190122092649	38.46680528387909	35.593567447933516	13.385437146094745
6	28.487714002246413	38.76167794020297	19.16298970201032	13.587618355540293
7	25.613540270587652	33.61248259896877	22.893177758824947	17.88079937161863
8	24.333786196474307	36.653917231604964	21.260866797057517	17.751429774863215
9	25.396655372738515	17.109030908379196	21.37721287974429	36.117100839138
10-11	23.65164824687169	27.518993785247257	28.734429093506346	20.094928874374705
12-13	24.831919656779355	25.086103020010057	29.016913464475273	21.06506385873531
14-15	21.902522508220216	26.350090102355882	27.18627376920472	24.56111362021918
16-17	20.917185180653366	29.759600653065792	27.74292559581366	21.58028857046718
18-19	20.90357602884996	28.392994647560005	30.109896217538555	20.593533106051478
20-21	22.107432328210287	27.270519947996124	29.983872577548897	20.63817514624469
22-23	21.63835254832769	27.43136987809362	29.872390852901837	21.05788672067686
24-25	21.358331740237936	27.6887257909576	29.976715048014324	20.97622742079014
26-27	21.312429820610586	27.90548898819222	29.845973752648913	20.93610743854828
28-29	20.94434974360988	27.98039289108197	29.99240732565219	21.082850039655955
30-31	21.55590072344149	27.67137630718695	29.94182251702252	20.830900452349038
32-33	21.04315008476849	27.967201498742643	29.874632290647902	21.115016125840967
34-35	21.277073663139916	28.019246079221322	29.9160536672053	20.78762659043346
36-37	21.103338821014326	27.982616907866376	29.85549538306232	21.058548888056976
38-39	21.028482060239632	27.81037978086597	29.982143077757918	21.178995081136488
40-41	21.26941814348665	27.752026060803725	29.86862124111984	21.109934554589792
42-43	21.32285423225075	27.762157737698363	29.672449366167335	21.24253866388355
44-45	21.338535357371967	28.116157750874343	29.31291704705476	21.23238984469893
46-47	21.51864629297596	27.951738221537532	28.848983958529328	21.680631526957182
48-49	21.49866190407763	28.076742895994204	28.795095529980586	21.629499669947585
50-51	21.436287785589734	28.246959626157377	28.692996479038335	21.623756109214558
52-53	21.538136611936654	28.270984859354897	28.68730995949724	21.503568569211204
54-55	21.538296842540355	28.265333617065576	28.520221513219575	21.676148027174495
56-57	21.684316504097048	28.43847576717385	28.246375811950575	21.630831916778533
58-59	21.417834939470392	28.601190395263547	28.53646896614127	21.44450569912479
60-61	21.707651454765184	28.703637906409757	28.18752176829881	21.40118887052625
62-63	21.476877101567876	28.78060867981252	28.185036311386032	21.55747790723358
64-65	21.465217350855085	28.702898607768475	28.245747244723162	21.586136796653282
66-67	21.601514628353605	28.607895312764285	28.12488614107335	21.665703917808756
68-69	21.655070218348033	28.46599484660168	28.20969213889468	21.669242796155604
70-71	21.589588869931298	28.268292985515497	28.29667279175882	21.845445352794382
72-73	21.610038779843215	28.310021655952017	28.323290332300182	21.756649231904586
74-75	21.57469766311392	28.374048006678347	28.23712396866537	21.814130361542368
76-77	21.616527509448954	28.324374314949186	28.204604305986347	21.85449386961551
78-79	21.66728977887452	28.33223668591324	28.166918134266112	21.833555400946132
80-81	21.637640018538427	28.28788617943952	28.193055705047886	21.881418096974166
82-83	21.695336254467847	28.275873400611545	28.264516952385932	21.764273392534676
84-85	21.82465645557942	28.223843226164373	28.174194990756813	21.777305327499395
86-87	21.65003201541337	28.246072542714767	28.244889415834823	21.85900602603704
88-89	21.753675022508038	28.204549538819023	28.21588149053121	21.82589394814173
90-91	21.74958344856423	28.17231207123768	28.27448906464299	21.803615415555104
92-93	21.741156531043643	28.085428689847795	28.299778378954976	21.873636400153586
94-95	21.643139707877314	28.178360326171543	28.30964755163604	21.8688524143151
96-97	21.58582219399907	28.183094450962326	28.425620184604682	21.805463170433917
98-99	21.82600898252978	28.695402890015444	28.469569078380758	21.009019049074023
100-101	22.23186579977793	31.467732539350497	25.760532389065105	20.539869271806477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2863.0
1	2508.5
2	3218.0
3	4565.0
4	6307.0
5	9529.5
6	12050.5
7	14751.0
8	17282.5
9	17911.0
10	16576.5
11	15313.5
12	17512.5
13	24459.5
14	40338.0
15	61288.0
16	77194.0
17	84595.5
18	83628.0
19	76347.5
20	66392.5
21	56587.5
22	50583.0
23	52057.5
24	60401.5
25	73963.0
26	92277.5
27	113284.5
28	135831.0
29	159406.0
30	181696.5
31	204462.5
32	229027.5
33	252228.5
34	275573.0
35	298650.0
36	320377.5
37	338853.0
38	351012.5
39	361291.0
40	372361.5
41	381971.5
42	387516.0
43	392747.5
44	399289.5
45	407210.5
46	414776.0
47	416282.0
48	407429.0
49	393968.0
50	380023.0
51	360143.5
52	335489.5
53	310748.0
54	285586.0
55	258955.5
56	231388.5
57	203941.0
58	177830.0
59	152460.0
60	127870.5
61	103817.0
62	81472.0
63	62205.5
64	46155.0
65	33944.0
66	25201.0
67	18734.5
68	14061.5
69	9885.0
70	6707.0
71	4785.5
72	3376.0
73	2385.5
74	1622.5
75	1080.0
76	757.0
77	490.5
78	299.5
79	186.0
80	118.5
81	85.0
82	67.5
83	45.5
84	23.0
85	11.5
86	8.5
87	7.5
88	9.0
89	10.5
90	8.5
91	6.5
92	6.0
93	4.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.0738657043376768E-5
2	0.007901428333526549
3	1.0369328521688384E-5
4	4.1477314086753536E-5
5	2.0738657043376768E-5
6	2.0738657043376768E-5
7	1.0369328521688384E-5
8	0.0
9	1.0369328521688384E-5
10-11	8.243616174742266E-4
12-13	5.184664260844192E-6
14-15	7.414069893007195E-4
16-17	8.399156102567591E-4
18-19	0.0
20-21	4.199696937425449E-4
22-23	0.0013274132775239014
24-25	0.00448605218953835
26-27	0.017224062222443576
28-29	0.3240788061233712
30-31	0.31178733664140185
32-33	0.3202444755210958
34-35	0.18551759220167915
36-37	0.04626449123172991
38-39	0.03629515790293682
40-41	0.03187592332711313
42-43	0.028033235124208754
44-45	0.01514744677134767
46-47	0.008251149460577686
48-49	0.006192073361031436
50-51	0.0026680005925417217
52-53	0.0019194717973866364
54-55	0.0016006510676598044
56-57	6.072549087679703E-4
58-59	0.002655434621753297
60-61	0.0025770664113520417
62-63	4.6630786932306325E-4
64-65	1.6101892777495994E-4
66-67	0.008148920527396205
68-69	0.007366512177418326
70-71	0.00965857045998124
72-73	0.010298226921024762
74-75	0.008395134527934086
76-77	0.005217645580434403
78-79	4.18770882976474E-4
80-81	5.671802886204467E-4
82-83	6.202786238788794E-4
84-85	0.0
86-87	0.0017036736815881214
88-89	0.0014250377069773067
90-91	0.001223245416044184
92-93	0.012409181975853764
94-95	1.5786589421449483E-4
96-97	7.318433149808985E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	165.0
20-21	596.0
22-23	1282.0
24-25	3110.0
26-27	4967.0
28-29	8566.0
30-31	18857.0
32-33	25889.0
34-35	38196.0
36-37	52727.0
38-39	63180.0
40-41	68101.0
42-43	83908.0
44-45	103402.0
46-47	103764.0
48-49	89363.0
50-51	77247.0
52-53	72382.0
54-55	79032.0
56-57	84975.0
58-59	86259.0
60-61	86064.0
62-63	85947.0
64-65	90794.0
66-67	98936.0
68-69	92442.0
70-71	86937.0
72-73	82643.0
74-75	84591.0
76-77	86289.0
78-79	90449.0
80-81	93016.0
82-83	89719.0
84-85	90625.0
86-87	96223.0
88-89	102413.0
90-91	109865.0
92-93	112418.0
94-95	129369.0
96-97	184148.0
98-99	785585.0
100-101	5899385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.10573016732033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40375760051239	76.08772070293722
2	7.418206295714458	12.62663726657183
3	1.8235071247397001	4.655727159488491
4	0.6640543036384987	2.2605930552762348
5	0.2899506983968625	1.2338232949794727
6	0.1428555536809852	0.7294695722686243
7	0.07965860002014864	0.4745582323174888
8	0.04802200091173641	0.326955796135124
9	0.033479867518021386	0.2564395713923724
>10	0.09479808381426584	1.1735138246235364
>50	0.0012753542957799303	0.07147954039976492
>100	3.5935139255831257E-4	0.045462621237122
>500	5.637535237205205E-5	0.03363229674451435
>1k	1.8790012204914554E-5	0.023987065628281706
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0369328521688384E-5	0.0	0.0	0.0	0.0
2	1.0369328521688384E-5	0.0	0.0	3.110798556506515E-5	0.0
3	1.0369328521688384E-5	0.0	0.0	3.110798556506515E-5	0.0
4	1.0369328521688384E-5	2.0738657043376768E-5	0.0	4.1477314086753536E-5	0.0
5	2.0738657043376768E-5	2.0738657043376768E-5	0.0	4.1477314086753536E-5	0.0
6	2.0738657043376768E-5	2.0738657043376768E-5	0.0	4.1477314086753536E-5	0.0
7	2.0738657043376768E-5	2.0738657043376768E-5	0.0	4.1477314086753536E-5	0.0
8	3.110798556506515E-5	2.0738657043376768E-5	0.0	4.1477314086753536E-5	0.0
9	3.110798556506515E-5	3.110798556506515E-5	0.0	5.184664260844192E-5	0.0
10-11	4.6661978347597725E-5	3.110798556506515E-5	0.0	7.258529965181868E-5	0.0
12-13	5.184664260844192E-5	3.110798556506515E-5	0.0	7.258529965181868E-5	0.0
14-15	6.740063539097449E-5	5.7031306869286116E-5	0.0	8.295462817350707E-5	1.0369328521688384E-5
16-17	7.776996391266287E-5	6.22159711301303E-5	0.0	1.1924727799941641E-4	1.0369328521688384E-5
18-19	1.5035526356448157E-4	6.22159711301303E-5	0.0	1.6590925634701415E-4	2.0738657043376768E-5
20-21	1.866479133903909E-4	6.22159711301303E-5	0.0	1.866479133903909E-4	2.0738657043376768E-5
22-23	1.918325776512351E-4	6.22159711301303E-5	0.0	2.0738657043376768E-4	3.110798556506515E-5
24-25	2.1775589895545606E-4	6.22159711301303E-5	0.0	2.2294056321630023E-4	4.1477314086753536E-5
26-27	2.2812522747714444E-4	6.22159711301303E-5	0.0	2.747872058247422E-4	4.1477314086753536E-5
28-29	2.6960254156389797E-4	6.22159711301303E-5	0.0	6.273443755621473E-4	4.1477314086753536E-5
30-31	2.799718700855864E-4	7.776996391266287E-5	0.0	0.0015191066284273482	4.1477314086753536E-5
32-33	2.955258628681189E-4	8.295462817350707E-5	0.0	0.0034218784121571666	4.1477314086753536E-5
34-35	3.214491841723399E-4	1.244319422602606E-4	0.0	0.006066057185187705	4.1477314086753536E-5
36-37	3.4218784121571664E-4	1.866479133903909E-4	0.0	0.01022934258664559	4.1477314086753536E-5
38-39	3.4218784121571664E-4	1.866479133903909E-4	0.0	0.01632650775739836	4.1477314086753536E-5
40-41	3.732958267807818E-4	2.3849455599883284E-4	0.0	0.023336173838059707	4.1477314086753536E-5
42-43	3.732958267807818E-4	2.3849455599883284E-4	0.0	0.030283623947590925	4.1477314086753536E-5
44-45	3.7848049104162603E-4	2.747872058247422E-4	0.0	0.03812802097424819	4.6661978347597725E-5
46-47	3.836651553024702E-4	3.1107985565065154E-4	0.0	0.046786410289857985	5.184664260844192E-5
48-49	3.836651553024702E-4	3.1107985565065154E-4	0.0	0.05555886221920636	5.184664260844192E-5
50-51	3.992191480850028E-4	3.318185126940283E-4	0.0	0.06418095888499026	5.184664260844192E-5
52-53	4.1477314086753536E-4	3.318185126940283E-4	0.0	0.07256456099477532	6.22159711301303E-5
54-55	4.1477314086753536E-4	3.318185126940283E-4	0.0	0.08095334776882121	7.776996391266287E-5
56-57	4.1477314086753536E-4	3.318185126940283E-4	0.0	0.08805115314191692	8.295462817350707E-5
58-59	4.1477314086753536E-4	3.318185126940283E-4	0.0	0.09392537774945339	8.295462817350707E-5
60-61	4.1995780512837954E-4	3.4737250547656087E-4	0.0	0.09950926115838257	8.295462817350707E-5
62-63	4.355117979109121E-4	3.5255716973740505E-4	0.0	0.1046731867621834	8.295462817350707E-5
64-65	4.458811264326005E-4	3.940344838241586E-4	0.0	0.10986303568728842	8.295462817350707E-5
66-67	4.458811264326005E-4	4.1477314086753536E-4	0.0	0.11499585330552417	8.295462817350707E-5
68-69	4.666197834759773E-4	4.1477314086753536E-4	0.0	0.12043975077941058	8.295462817350707E-5
70-71	4.769891119976657E-4	4.1477314086753536E-4	0.0	0.12555182974060297	8.295462817350707E-5
72-73	4.769891119976657E-4	4.1477314086753536E-4	0.0	0.12993805570527714	1.1406261373857222E-4
74-75	4.8735844051935403E-4	4.355117979109121E-4	0.0	0.13334438012465177	1.1406261373857222E-4
76-77	4.8735844051935403E-4	4.4588112643260047E-4	0.0	0.1367610738725481	1.1406261373857222E-4
78-79	4.8735844051935403E-4	4.562504549542889E-4	0.0	0.13945191462392623	1.1406261373857222E-4
80-81	4.8735844051935403E-4	4.6143511921513305E-4	0.0	0.14058217143279028	1.1924727799941641E-4
82-83	4.925431047801982E-4	4.666197834759773E-4	0.0	0.14104879121626623	1.244319422602606E-4
84-85	5.029124333018867E-4	4.666197834759773E-4	0.0	0.14132876308635184	1.244319422602606E-4
86-87	5.184664260844192E-4	4.8735844051935403E-4	0.0	0.14141690237878618	1.244319422602606E-4
88-89	5.184664260844192E-4	5.029124333018867E-4	0.0	0.14145837969287292	1.244319422602606E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17740	0.0	52.929493	1
GTATCAA	32435	0.0	37.62881	1
CTTATAC	8005	0.0	31.519165	1
ATCAACG	40865	0.0	29.450039	3
TCAACGC	41215	0.0	29.200098	4
TATCAAC	41790	0.0	29.083763	2
CAACGCA	42210	0.0	28.481985	5
AACGCAG	43570	0.0	27.670671	6
GTGGTAT	7775	0.0	25.645449	1
TGGTATC	7500	0.0	25.513319	2
ACGCAGA	50110	0.0	24.059294	7
CGCAGAG	50540	0.0	23.837843	8
TATACAC	13470	0.0	22.564066	3
GCAGAGT	57310	0.0	20.911201	9
TTATACA	13360	0.0	20.53328	2
GAGTACT	31345	0.0	20.04848	12-13
GTACATG	43320	0.0	19.388447	1
ACATCTC	13425	0.0	19.203053	8
TACATGG	43110	0.0	19.129372	2
CAGAGTA	56625	0.0	18.74587	10-11
>>END_MODULE