FastQCFastQC Report
Fri 27 May 2016
SRR2139949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9435356
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164100.042.445071
GTATCAA275950.033.5044251
TCAACGC333400.027.1505434
ATCAACG333600.027.1336923
CAACGCA342250.026.4610025
TATCAAC347900.026.341212
AACGCAG352950.025.7073866
CTTATAC64850.022.340311
ACGCAGA407750.022.2524137
CGCAGAG411400.022.0654058
GAGTACT259100.019.68516212-13
GTACATG292350.019.6464541
GCAGAGT462150.019.540749
TACATGG294350.019.0178592
AGAGTAC395100.018.85941510-11
GTACTTT282600.017.95797514-15
ACATGGG304300.017.9435543
AGTACTT279750.017.7801112-13
CAGAGTA447050.017.77039310-11
TATACAC105050.017.5025863
Produced by FastQC (version 0.11.5)