##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9435356
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66990318118363	34.0	31.0	34.0	31.0	34.0
2	32.826394785739936	34.0	31.0	34.0	31.0	34.0
3	32.87145646650747	34.0	31.0	34.0	31.0	34.0
4	36.3285568663228	37.0	37.0	37.0	35.0	37.0
5	36.2646701406921	37.0	37.0	37.0	35.0	37.0
6	36.30024187746599	37.0	37.0	37.0	35.0	37.0
7	36.2579631335585	37.0	37.0	37.0	35.0	37.0
8	36.239443535569826	37.0	37.0	37.0	35.0	37.0
9	37.91981235260228	39.0	38.0	39.0	35.0	39.0
10-11	37.89683870963639	39.0	38.0	39.0	35.0	39.0
12-13	37.869583299241704	39.0	38.0	39.0	35.0	39.0
14-15	39.2910839294246	41.0	39.0	41.0	36.0	41.0
16-17	39.277202153262685	41.0	39.0	41.0	36.0	41.0
18-19	39.30764763936835	41.0	39.0	41.0	36.0	41.0
20-21	39.2281402826482	41.0	39.0	41.0	36.0	41.0
22-23	39.22606028266304	40.5	39.0	41.0	36.0	41.0
24-25	39.13307003713038	40.5	39.0	41.0	36.0	41.0
26-27	38.99319343066715	40.0	39.0	41.0	35.0	41.0
28-29	38.86606800267762	40.0	39.0	41.0	35.0	41.0
30-31	38.74952488525781	40.0	38.0	41.0	35.0	41.0
32-33	38.65326623369303	40.0	38.0	41.0	35.0	41.0
34-35	38.50658847048528	40.0	38.0	41.0	34.5	41.0
36-37	38.64070536023688	40.0	38.0	41.0	35.0	41.0
38-39	38.6958257639364	40.0	38.0	41.0	35.0	41.0
40-41	38.6798315767484	40.0	38.0	41.0	35.0	41.0
42-43	38.64440345909256	40.0	38.0	41.0	35.0	41.0
44-45	38.57006708280477	40.0	38.0	41.0	34.0	41.0
46-47	38.49958764656797	40.0	38.0	41.0	34.0	41.0
48-49	38.41086099568319	40.0	38.0	41.0	34.0	41.0
50-51	38.31431445838773	40.0	38.0	41.0	34.0	41.0
52-53	38.20331880355533	40.0	37.5	41.0	34.0	41.0
54-55	38.063671198698295	40.0	37.0	41.0	33.0	41.0
56-57	37.91309356411938	40.0	37.0	41.0	33.0	41.0
58-59	37.72054793782114	39.5	36.0	41.0	33.0	41.0
60-61	37.48820150039174	39.0	36.0	41.0	33.0	41.0
62-63	37.286010657612735	39.0	35.5	40.0	32.5	41.0
64-65	37.0452874726267	38.5	35.0	40.0	32.0	41.0
66-67	36.76181784263768	38.0	35.0	40.0	32.0	41.0
68-69	36.40357392751625	37.0	35.0	40.0	31.0	41.0
70-71	36.11845304925565	37.0	35.0	39.0	31.0	41.0
72-73	35.78860441999976	36.0	34.5	39.0	31.0	40.0
74-75	35.45732511384732	36.0	34.0	38.5	31.0	40.0
76-77	34.44202338098191	35.0	33.5	37.0	29.5	39.0
78-79	34.724016146311676	35.0	34.0	37.0	30.5	39.0
80-81	34.557594503765614	35.0	34.0	37.0	30.5	39.0
82-83	34.28550333395876	35.0	34.0	36.0	30.0	38.0
84-85	34.025690452876404	35.0	34.0	36.0	30.0	37.0
86-87	33.80466044111817	35.0	33.5	35.5	30.0	37.0
88-89	33.626164886736056	35.0	33.0	35.0	30.0	36.5
90-91	33.44290211142361	35.0	33.0	35.0	30.0	36.0
92-93	33.309704815636564	35.0	33.0	35.0	29.0	36.0
94-95	33.22258118146462	34.0	33.0	35.0	29.0	35.5
96-97	33.20522543426608	34.0	33.0	35.0	29.0	35.0
98-99	33.22019478666361	34.0	33.0	35.0	30.0	35.0
100-101	32.214986347605105	33.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	50.0
21	208.0
22	1117.0
23	3875.0
24	9824.0
25	20576.0
26	36214.0
27	57599.0
28	82343.0
29	110761.0
30	142759.0
31	178646.0
32	226460.0
33	295585.0
34	413845.0
35	608095.0
36	1051769.0
37	2162694.0
38	2804987.0
39	1180912.0
40	47029.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.17124059759907	21.120305370565774	15.596157686048093	24.11229634578706
2	13.774273336787275	21.516832877000397	41.31300510714143	23.395888679070893
3	17.279454002583474	26.243885233371163	31.11999165691258	25.356669107132788
4	12.06872449420292	18.763671319203144	38.716338706916694	30.451265479677236
5	13.118031794454815	37.78298349315066	35.49736756090602	13.601617151488506
6	29.62389548417675	38.162269658929674	18.61727315853265	13.596561698360931
7	26.21414602692257	32.937072008729714	22.755463598829763	18.093318365517952
8	24.456650072344914	36.668324968342475	20.880918536619074	17.994106422693537
9	25.14486999748605	17.243758476097774	21.303191951633835	36.30817957478234
10-11	24.000637607040428	27.08923218323656	28.61917112462552	20.290959085097487
12-13	25.00004239373692	24.52195762406845	28.900266190274117	21.577733791920515
14-15	22.041902154469966	25.964870927927446	26.753744360070314	25.239482557532277
16-17	21.48027474304179	29.29393047738124	27.331876108935294	21.893918670641675
18-19	21.472724505572444	28.170781261459553	29.355076798374114	21.001417434593883
20-21	22.29278773068115	27.173023091458898	29.33139071803246	21.202798459827495
22-23	21.96383629957704	27.145463818200717	29.285024969275657	21.60567491294659
24-25	21.847850212463143	27.374982460174724	29.231476552984216	21.545690774377917
26-27	21.819300797333398	27.515119718811842	29.170805301639135	21.49477418221563
28-29	21.58794211561376	27.59794425342326	29.238601259348656	21.57551237161432
30-31	22.023641468575946	27.37569937839366	29.122662169659574	21.47799698337082
32-33	21.656409017847476	27.585718687798256	29.093044252780416	21.664828041573855
34-35	21.88905126455133	27.598647491572216	29.103425646543602	21.40887559733285
36-37	21.751804468966274	27.579620131596666	29.088741229394177	21.57983417004288
38-39	21.651405628373837	27.559971687222124	29.15491658196192	21.63370610244212
40-41	21.875626470885916	27.442480626404787	29.028756381740394	21.653136520968907
42-43	21.827347382230233	27.54995506834863	28.791870602516074	21.83082694690506
44-45	21.903934076305838	27.67542431307355	28.579035806481713	21.841605804138904
46-47	22.082698124296154	27.65070143009394	28.223243046913943	22.043357398695967
48-49	21.964007075619293	27.799846679601547	28.19659388476498	22.039552360014177
50-51	21.918920544709433	27.929679965274335	28.11749113289655	22.03390835711968
52-53	22.079082422304605	27.86555255318684	28.06410416317672	21.99126086133184
54-55	22.04163092886602	27.81012610411246	27.986385137538395	22.16185782948313
56-57	22.14893952067866	27.930169159490138	27.85176838050989	22.06912293932131
58-59	22.013218048258157	28.044505498700385	27.9900310685503	21.95224538449116
60-61	22.167542790969037	28.091614630712503	27.845345568212043	21.895497010106414
62-63	22.00154953640477	28.212855958977656	27.82000004624066	21.965594458376913
64-65	22.021663650981633	28.165497413392703	27.754644966333853	22.058193969291807
66-67	22.08591343424216	28.10772399463645	27.64593928558486	22.160423285536538
68-69	22.08691069848266	27.991838472638914	27.769170927530446	22.152079901347985
70-71	22.115168569446332	27.79962755813442	27.825873999088678	22.25932987333057
72-73	22.138498913602497	27.832239718483613	27.806176382274543	22.223084985639343
74-75	22.024890046048036	27.938846489566632	27.773027582530553	22.263235881854783
76-77	22.119993616632073	27.872312598297142	27.71690194553181	22.290791839538976
78-79	22.112853605145226	27.867725736080136	27.714186860125178	22.305233798649454
80-81	22.140115564812557	27.890179608394067	27.706950824506855	22.26275400228652
82-83	22.142571276564873	27.85717650223439	27.787930318029968	22.212321903170775
84-85	22.214453678367978	27.845645437475458	27.747547145212504	22.192353738944067
86-87	22.09040566949014	27.823591318243047	27.828550775019	22.25745223724781
88-89	22.127217451852218	27.78291356814289	27.75070771779342	22.33916126221147
90-91	22.126301827552112	27.769803708588718	27.838096865269137	22.265797598590034
92-93	22.176600606691476	27.686783518810564	27.87759619723281	22.25901967726515
94-95	22.050315524199053	27.72784354532491	27.898573874160913	22.323267056315125
96-97	22.02834730761257	27.785967064187776	27.952733779325577	22.232951848874073
98-99	22.259493373834392	28.373648530678985	27.949588950570813	21.41726914491581
100-101	22.710891010622593	31.063894172465652	25.242502919443226	20.98271189746853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2380.0
1	2030.5
2	2497.5
3	3543.0
4	4710.0
5	6975.0
6	8938.0
7	11403.0
8	14047.0
9	14960.0
10	13880.0
11	12581.0
12	13849.0
13	18551.5
14	29842.5
15	45296.0
16	57629.5
17	64073.5
18	64209.0
19	59751.5
20	53595.0
21	47233.5
22	43352.5
23	44861.5
24	51702.0
25	63625.0
26	79486.0
27	97402.5
28	117683.0
29	139677.0
30	160924.5
31	182547.5
32	205160.0
33	226915.5
34	247816.5
35	267236.0
36	288275.5
37	308192.5
38	323669.5
39	335795.0
40	347828.0
41	359675.0
42	366105.0
43	372146.5
44	379582.0
45	387930.0
46	396288.5
47	401677.0
48	399018.5
49	386558.0
50	372868.5
51	357377.5
52	337996.5
53	319160.0
54	300045.5
55	277815.5
56	253499.0
57	228032.5
58	202203.5
59	175740.5
60	148560.5
61	122770.0
62	97793.5
63	75650.5
64	57437.5
65	43242.0
66	33011.0
67	25289.0
68	19306.5
69	13941.5
70	9780.0
71	7119.0
72	5058.0
73	3503.5
74	2392.0
75	1578.5
76	1023.5
77	653.5
78	434.5
79	293.0
80	182.5
81	113.5
82	76.0
83	53.0
84	38.0
85	28.5
86	25.5
87	20.0
88	13.0
89	8.5
90	4.5
91	1.5
92	0.5
93	1.0
94	2.5
95	3.0
96	3.0
97	3.0
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0076732663823177415
3	0.0
4	1.0598434229720637E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.213786528033495E-4
12-13	0.0
14-15	6.941974420467018E-4
16-17	7.100950933912828E-4
18-19	0.0
20-21	4.504461775375846E-4
22-23	0.0012030431586168417
24-25	0.004410273073825162
26-27	0.016608353598847807
28-29	0.32173033337511003
30-31	0.3090432613824092
32-33	0.3176295298501831
34-35	0.18346737164354174
36-37	0.045589652418404916
38-39	0.03554261856648182
40-41	0.031328626239739386
42-43	0.027490818355644497
44-45	0.014854090990927844
46-47	0.007676597545464829
48-49	0.005691904492755791
50-51	0.0024287041197317244
52-53	0.0019630584268209086
54-55	0.0015247386262666723
56-57	5.89432617363402E-4
58-59	0.0023952556454602037
60-61	0.0024571045679631304
62-63	4.3276328725570215E-4
64-65	2.212866382642276E-4
66-67	0.007682332182110892
68-69	0.006986285037744854
70-71	0.009316957343000951
72-73	0.00979845602462268
74-75	0.007737616908200273
76-77	0.004972487682310806
78-79	4.101359722507128E-4
80-81	4.5999730610028343E-4
82-83	5.699511532208202E-4
84-85	0.0
86-87	0.0016352536013612279
88-89	0.0012757316948957364
90-91	0.001334343399188912
92-93	0.011956211370461708
94-95	9.218691762687614E-5
96-97	4.3342279179756034E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	560.0
22-23	1178.0
24-25	3437.0
26-27	5332.0
28-29	7538.0
30-31	16682.0
32-33	22431.0
34-35	33103.0
36-37	45416.0
38-39	54228.0
40-41	58554.0
42-43	70652.0
44-45	86169.0
46-47	85541.0
48-49	74810.0
50-51	66691.0
52-53	63187.0
54-55	68991.0
56-57	72929.0
58-59	73628.0
60-61	72267.0
62-63	72843.0
64-65	78487.0
66-67	85179.0
68-69	81575.0
70-71	77997.0
72-73	75617.0
74-75	77351.0
76-77	79593.0
78-79	84423.0
80-81	85275.0
82-83	84738.0
84-85	86181.0
86-87	90583.0
88-89	96905.0
90-91	105107.0
92-93	108658.0
94-95	123084.0
96-97	184030.0
98-99	803181.0
100-101	5971062.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.71124086716276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.05770913050914	77.1895799922995
2	7.024407358241749	12.04141342062658
3	1.6468228420696636	4.234536878465354
4	0.5972248895537156	2.0475554544161274
5	0.26309216073319514	1.1274977779432591
6	0.14087698034417195	0.7244844476950725
7	0.08884204848153542	0.5330333551573123
8	0.0461104659790396	0.3161748204821262
9	0.029206055728281794	0.22529585495758855
>10	0.10346107209467945	1.3762833942015464
>50	0.0017282732054675419	0.09444921494744683
>100	4.991334082200371E-4	0.07819387367256288
>500	1.9589651105900178E-5	0.011501515135424216
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1196868459441275E-5
2	0.0	0.0	0.0	0.0	2.1196868459441275E-5
3	0.0	0.0	0.0	3.179530268916191E-5	2.1196868459441275E-5
4	0.0	2.1196868459441275E-5	0.0	3.179530268916191E-5	3.179530268916191E-5
5	0.0	4.239373691888255E-5	0.0	3.179530268916191E-5	3.179530268916191E-5
6	3.179530268916191E-5	4.239373691888255E-5	0.0	3.179530268916191E-5	5.299217114860319E-5
7	3.179530268916191E-5	4.239373691888255E-5	0.0	3.179530268916191E-5	5.299217114860319E-5
8	3.179530268916191E-5	5.299217114860319E-5	0.0	3.179530268916191E-5	5.299217114860319E-5
9	3.179530268916191E-5	1.377796449863683E-4	0.0	3.179530268916191E-5	7.418903960804447E-5
10-11	3.7094519804022234E-5	1.377796449863683E-4	0.0	4.239373691888255E-5	7.418903960804447E-5
12-13	4.239373691888255E-5	1.5367729633094927E-4	0.0	5.299217114860319E-5	7.418903960804447E-5
14-15	4.239373691888255E-5	1.7487416479039053E-4	0.0	6.359060537832382E-5	1.1128355941206669E-4
16-17	7.418903960804447E-5	1.907718161349715E-4	0.0	1.0598434229720637E-4	1.1658277652692702E-4
18-19	7.418903960804447E-5	2.1196868459441278E-4	0.0	1.642757305606699E-4	1.1658277652692702E-4
20-21	7.418903960804447E-5	2.543624215132953E-4	0.0	2.1726790170927308E-4	1.1658277652692702E-4
22-23	7.418903960804447E-5	2.7555928997273656E-4	0.0	2.861577242024572E-4	1.2718121075664768E-4
24-25	7.418903960804447E-5	3.2325224400647947E-4	0.0	2.967561584321779E-4	1.377796449863683E-4
26-27	7.418903960804447E-5	3.285514611213398E-4	0.0	3.3385067823620007E-4	1.430788621012286E-4
28-29	7.418903960804447E-5	3.3385067823620007E-4	0.0	6.359060537832383E-4	1.4837807921608894E-4
30-31	7.418903960804447E-5	3.709451980402223E-4	0.0	0.0014890800092757497	1.5367729633094927E-4
32-33	7.418903960804447E-5	3.709451980402223E-4	0.0	0.0032060263544904932	1.907718161349715E-4
34-35	7.418903960804447E-5	4.6103188899284774E-4	0.0	0.005548280319258754	1.907718161349715E-4
36-37	7.418903960804447E-5	5.352209286008922E-4	0.0	0.00915174795736377	2.1196868459441278E-4
38-39	7.418903960804447E-5	5.670162312900542E-4	0.0	0.014482760374913252	2.225671188241334E-4
40-41	7.418903960804447E-5	6.518037051278193E-4	0.0	0.020486773366049992	2.225671188241334E-4
42-43	7.418903960804447E-5	6.835990078169812E-4	0.0	0.026199329415869416	2.225671188241334E-4
44-45	7.948825672290478E-5	7.577880474250256E-4	0.0	0.032998224974235206	2.225671188241334E-4
46-47	8.47874738377651E-5	8.160794356884891E-4	0.0	0.041090129508626914	2.225671188241334E-4
48-49	9.008669095262543E-5	8.266778699182098E-4	0.0	0.04933571133934957	2.3316555305385404E-4
50-51	1.0598434229720637E-4	9.273629951005558E-4	0.0	0.057353426834133234	2.4376398728357467E-4
52-53	1.0598434229720637E-4	9.326622122154161E-4	0.0	0.06484122061743086	2.6496085574301596E-4
54-55	1.0598434229720637E-4	9.48559863559997E-4	0.0	0.07280594394106593	2.9145694131731755E-4
56-57	1.0598434229720637E-4	9.750559491342987E-4	0.0	0.07880465771508781	3.020553755470382E-4
58-59	1.0598434229720637E-4	9.856543833640194E-4	0.0	0.08399789048765091	3.073545926618985E-4
60-61	1.1128355941206669E-4	0.0010068512518234605	0.0	0.08882547727928866	3.073545926618985E-4
62-63	1.2718121075664765E-4	0.001033347337397762	0.0	0.09379614293302765	3.1795302689161914E-4
64-65	1.2718121075664765E-4	0.0012082215021881528	0.0	0.09863432815889511	3.1795302689161914E-4
66-67	1.2718121075664765E-4	0.0012347175877624544	0.0	0.10343011964784371	3.1795302689161914E-4
68-69	1.2718121075664765E-4	0.0012612136733367559	0.0	0.10859155711771766	3.1795302689161914E-4
70-71	1.377796449863683E-4	0.0012824105417961972	0.0	0.11356752198857151	3.285514611213398E-4
72-73	1.4837807921608894E-4	0.0012930089760259179	0.0	0.1175207379562573	3.709451980402223E-4
74-75	1.4837807921608894E-4	0.0013195050616002196	0.0	0.12042470893520074	3.709451980402223E-4
76-77	1.4837807921608894E-4	0.0013460011471745211	0.0	0.1235830423356575	3.709451980402223E-4
78-79	1.4837807921608894E-4	0.0013565995814042416	0.0	0.12621675324174308	3.709451980402223E-4
80-81	1.4837807921608894E-4	0.0013565995814042416	0.0	0.1272024076251071	3.81543632269943E-4
82-83	1.5897651344580957E-4	0.0013565995814042416	0.0	0.12758395125737704	4.133389349591049E-4
84-85	1.5897651344580957E-4	0.0013671980156339622	0.0	0.12779591994197145	4.133389349591049E-4
86-87	1.6427573056066987E-4	0.001377796449863683	0.0	0.12783831367889031	4.133389349591049E-4
88-89	1.8017338190525086E-4	0.0014519854894717275	0.0	0.12788070741580923	4.133389349591049E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16410	0.0	42.44507	1
GTATCAA	27595	0.0	33.504425	1
TCAACGC	33340	0.0	27.150543	4
ATCAACG	33360	0.0	27.133692	3
CAACGCA	34225	0.0	26.461002	5
TATCAAC	34790	0.0	26.34121	2
AACGCAG	35295	0.0	25.707386	6
CTTATAC	6485	0.0	22.34031	1
ACGCAGA	40775	0.0	22.252413	7
CGCAGAG	41140	0.0	22.065405	8
GAGTACT	25910	0.0	19.685162	12-13
GTACATG	29235	0.0	19.646454	1
GCAGAGT	46215	0.0	19.54074	9
TACATGG	29435	0.0	19.017859	2
AGAGTAC	39510	0.0	18.859415	10-11
GTACTTT	28260	0.0	17.957975	14-15
ACATGGG	30430	0.0	17.943554	3
AGTACTT	27975	0.0	17.78011	12-13
CAGAGTA	44705	0.0	17.770393	10-11
TATACAC	10505	0.0	17.502586	3
>>END_MODULE