FastQCFastQC Report
Fri 27 May 2016
SRR2139948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7897385
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.042.7125781
GTATCAA138600.033.321281
TCAACGC161450.027.8679454
ATCAACG163350.027.5439723
CAACGCA166050.027.071215
AACGCAG171300.026.3135936
TATCAAC178050.025.3390832
ACGCAGA201850.022.2496787
GTACATG198500.022.2114121
CGCAGAG204800.021.929198
TACATGG198950.021.5422842
ACATGGG200150.020.8798483
CATGGGG147800.018.9704974
GCAGAGT250250.017.9135239
TATACCG4550.017.1425195
AGAGTAC218350.017.12764210-11
GAGTACT148150.016.54280912-13
CAGAGTA242500.016.14987210-11
ATGGGGG97050.015.1432795
AGTACTT162900.014.62910512-13
Produced by FastQC (version 0.11.5)