##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7897385
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72823675178556	31.0	31.0	34.0	30.0	34.0
2	31.923260800885355	33.0	31.0	34.0	30.0	34.0
3	31.987623498157934	33.0	31.0	34.0	30.0	34.0
4	35.69584615667085	37.0	35.0	37.0	33.0	37.0
5	35.44893771799146	37.0	35.0	37.0	33.0	37.0
6	35.4766551206507	37.0	35.0	37.0	33.0	37.0
7	35.43468629172821	37.0	35.0	37.0	33.0	37.0
8	35.42422586716996	37.0	35.0	37.0	33.0	37.0
9	37.19328359957125	39.0	37.0	39.0	34.0	39.0
10-11	37.03397073841531	39.0	37.0	39.0	33.0	39.0
12-13	37.038858115692726	39.0	37.0	39.0	33.0	39.0
14-15	38.138119516776754	40.0	37.5	41.0	33.0	41.0
16-17	38.06488350257712	40.0	37.0	41.0	33.0	41.0
18-19	38.132820547560996	40.0	37.5	41.0	33.5	41.0
20-21	38.17367027451449	40.0	37.5	41.0	33.5	41.0
22-23	38.15857551845205	40.0	37.5	41.0	34.0	41.0
24-25	38.112339166349784	40.0	37.0	41.0	33.0	41.0
26-27	37.98931429621015	40.0	37.0	41.0	33.0	41.0
28-29	37.817453619003516	40.0	37.0	41.0	33.0	41.0
30-31	37.71840254805532	39.0	37.0	41.0	33.0	41.0
32-33	37.626557923376524	39.0	37.0	41.0	32.5	41.0
34-35	37.55850512943731	39.0	37.0	41.0	32.0	41.0
36-37	37.516206277781734	39.0	37.0	40.5	32.0	41.0
38-39	37.43340412012134	39.0	36.0	40.0	32.0	41.0
40-41	37.42383542198427	39.0	36.0	40.0	32.0	41.0
42-43	37.37877956913621	39.0	36.0	40.0	32.0	41.0
44-45	37.315456811651984	39.0	36.0	40.0	31.0	41.0
46-47	37.26491599255316	39.0	36.0	40.0	31.0	41.0
48-49	37.28384097494437	39.0	36.0	40.0	31.0	41.0
50-51	37.189590393970676	39.0	36.0	40.0	31.0	41.0
52-53	37.01149705089753	39.0	36.0	40.0	31.0	41.0
54-55	36.822857733094345	39.0	35.0	40.0	31.0	41.0
56-57	36.715199040677234	39.0	35.0	40.0	31.0	41.0
58-59	36.535471112122515	38.0	35.0	40.0	30.0	41.0
60-61	36.369518974988196	38.0	35.0	40.0	30.0	41.0
62-63	36.238097718584484	38.0	34.5	40.0	30.0	41.0
64-65	35.99116499868827	38.0	34.0	40.0	29.0	41.0
66-67	35.860582611328155	37.0	34.0	40.0	29.0	41.0
68-69	35.60118597791157	37.0	34.0	39.0	29.0	41.0
70-71	35.2938645648989	36.0	34.0	39.0	29.0	40.5
72-73	35.0015305297228	36.0	34.0	39.0	29.0	40.0
74-75	34.651829886571946	35.5	33.0	38.5	29.0	40.0
76-77	32.50085850264195	33.5	30.5	36.0	25.5	38.5
78-79	33.78853639603219	35.0	32.5	37.0	28.0	39.0
80-81	33.792325267098235	35.0	33.0	36.5	29.0	39.0
82-83	33.6191941682747	35.0	33.0	36.0	29.0	38.5
84-85	33.40968414754415	35.0	33.0	36.0	29.0	37.0
86-87	33.16187498933063	35.0	32.0	35.0	28.5	37.0
88-89	32.99889432841637	34.0	32.0	35.0	28.0	36.0
90-91	32.830538225609615	34.0	32.0	35.0	28.0	36.0
92-93	32.791443616508644	34.0	32.0	35.0	28.5	36.0
94-95	32.753518511995765	34.0	32.0	35.0	29.0	35.5
96-97	32.779029124653874	34.0	32.0	35.0	29.0	35.0
98-99	32.919715153910204	34.0	32.0	35.0	29.0	35.0
100-101	31.75415144749384	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	15.0
20	64.0
21	348.0
22	1448.0
23	4944.0
24	12208.0
25	25823.0
26	46454.0
27	74067.0
28	108154.0
29	147464.0
30	192160.0
31	246225.0
32	314941.0
33	410351.0
34	553881.0
35	775633.0
36	1173734.0
37	1697855.0
38	1586998.0
39	519664.0
40	4952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03445634219428	21.052601589006994	13.689392121569355	24.223549947229365
2	14.857391258983085	22.31717962828506	40.310436093561606	22.514993019170248
3	17.983317241488468	26.81485936782792	31.008214754902735	24.193608635780876
4	12.370146873952184	18.431799694683708	39.46078853453245	29.73726489683166
5	12.521437478537198	38.413251780589626	36.45162752627959	12.61368321459359
6	29.361263253595972	39.05551014671312	18.93160837416436	12.651618225526551
7	25.753220844621357	32.89696526128586	24.116159969407594	17.233653924685196
8	24.12796079264619	37.09207229716913	21.83492218980324	16.945044720381436
9	25.45412183906445	16.182065329219736	21.508765243178594	36.85504758853722
10-11	23.70106709744367	26.957232213433418	29.89053616985175	19.45116451927116
12-13	24.45837864914991	24.551663069891898	29.81447074735041	21.17548753360778
14-15	21.55396511338659	26.04933949967866	27.15115365093342	25.245541736001332
16-17	21.321032215093982	29.162330315667784	27.833877922882067	21.682759546356166
18-19	21.33812759156901	28.504563579984637	29.282595387688566	20.87471344075779
20-21	22.02744072793522	27.76691875045073	29.130128911790386	21.075511609823664
22-23	21.7584532203489	27.714737525610015	28.95123088280571	21.575578371235373
24-25	21.565382361790743	27.807978209574486	29.046333159136616	21.580306269498156
26-27	21.544350968782815	27.991435779021955	28.922505170266245	21.541708081928984
28-29	21.519529752583193	28.13334985185034	28.864611405376273	21.482508990190198
30-31	21.80645515532444	27.94059844249456	28.768437474718866	21.484508927462134
32-33	21.532748273575606	28.136903702803632	28.7260201479877	21.604327875633057
34-35	21.66859674236677	28.149900184831527	28.764454124835915	21.417048947965792
36-37	21.675875229817073	28.045621047207476	28.721027983894427	21.557475739081024
38-39	21.5333969411756	28.202235804619402	28.754581429820934	21.50978582438406
40-41	21.777041653344796	28.0948259595679	28.61182379487287	21.516308592214436
42-43	21.674652835952728	28.189780829740975	28.397560354981326	21.738005979324967
44-45	21.783056379524258	28.24513505469406	28.23301574774184	21.738792818039844
46-47	21.821866135078118	28.227892400730653	28.055605206098143	21.894636258093087
48-49	21.74420808412098	28.336380519184516	28.100326468525534	21.81908492816897
50-51	21.73296403596757	28.41463210723805	28.023875420210352	21.82852843658403
52-53	21.91864364876487	28.29541194977665	27.882033452067194	21.903910949391285
54-55	21.84893817024243	28.212061979614866	27.97462473235714	21.964375117785565
56-57	21.826347789324867	28.369154533003794	27.905687343551268	21.898810334120075
58-59	21.83373274100052	28.40068321015626	27.880096049957505	21.88548799888572
60-61	21.91437491728394	28.363245057404484	27.891733112929217	21.83064691238236
62-63	21.928511801182495	28.35166225254727	27.878944377913832	21.840881568356398
64-65	21.875605442734514	28.327982120713617	27.83855374637566	21.957858690176206
66-67	21.925336053066687	28.379434382434294	27.703790868530014	21.991438695969006
68-69	21.89648092243118	28.342323541265575	27.78659704358511	21.974598492718137
70-71	21.86366372655258	28.157684656410975	27.839056785751794	22.139594831284647
72-73	21.89790944457045	28.20372862112078	27.816390359983128	22.081971574325642
74-75	21.77219449200876	28.322263803107113	27.7767961773013	22.12874552758283
76-77	21.898254471260298	28.249778243417623	27.712783741728515	22.139183543593564
78-79	21.89959046875971	28.270448989291413	27.681207978772644	22.148752563176238
80-81	21.915850953801673	28.25268637698771	27.687630100743494	22.143832568467122
82-83	21.936151723654486	28.225559856852616	27.763060592807786	22.075227826685104
84-85	22.03598273420864	28.187468989397686	27.719527879419374	22.057020396974295
86-87	21.858639583397952	28.24825069991611	27.775395109909912	22.117714606776023
88-89	21.89891538540514	28.26544379518245	27.718964484558654	22.11667633485376
90-91	21.956860440967247	28.232987594647895	27.785543118994205	22.024608845390656
92-93	21.97008699798526	28.196299112667138	27.769047307600648	22.064566581746956
94-95	21.796969674770388	28.248093467608328	27.83939245437682	22.11554440324447
96-97	21.798469596104642	28.22599766068672	27.886891282320857	22.088641460887782
98-99	22.01941312248311	28.82166640941124	27.943643055336842	21.215277412768813
100-101	25.968774300956955	35.88751694338015	28.560631066466023	9.583077689196877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1509.0
1	1441.5
2	2148.0
3	3069.5
4	4010.0
5	6440.5
6	8616.5
7	10031.0
8	10906.0
9	10895.0
10	10197.0
11	9169.0
12	9584.5
13	12081.0
14	18096.0
15	26888.5
16	34186.0
17	38497.5
18	40540.5
19	40675.0
20	39994.0
21	39915.5
22	41558.5
23	46958.5
24	56682.0
25	70862.0
26	89002.5
27	108240.5
28	127878.5
29	147831.0
30	165378.5
31	181229.5
32	198085.5
33	215965.5
34	234035.5
35	251244.0
36	270108.0
37	286256.5
38	297107.0
39	306386.5
40	316144.5
41	324699.5
42	328714.5
43	332479.5
44	336777.0
45	340556.5
46	341822.0
47	339889.5
48	335953.0
49	327770.0
50	318403.0
51	307856.5
52	292931.5
53	275444.5
54	258029.5
55	238245.5
56	217311.0
57	196560.0
58	173371.0
59	149350.5
60	124825.0
61	101173.0
62	80262.0
63	62420.0
64	47445.0
65	35712.0
66	27220.0
67	21015.5
68	15939.0
69	11513.0
70	8201.5
71	5908.0
72	4130.0
73	2813.0
74	1904.0
75	1305.5
76	901.0
77	606.5
78	415.5
79	304.5
80	216.5
81	145.5
82	105.5
83	77.0
84	58.0
85	45.5
86	38.5
87	33.0
88	24.0
89	15.0
90	8.5
91	7.0
92	6.5
93	4.0
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.444840285740153E-4
3	1.2662419269163146E-4
4	1.2662419269163148E-5
5	1.2662419269163148E-5
6	0.0
7	0.0
8	0.0011522801534938464
9	0.0
10-11	3.4821652990198654E-4
12-13	4.0519741661322075E-4
14-15	6.331209634581574E-6
16-17	0.0
18-19	2.722420142870076E-4
20-21	0.0
22-23	0.0
24-25	1.7102921007878936E-4
26-27	0.0
28-29	5.328525480913698E-4
30-31	4.44850684914357E-4
32-33	0.0010837549405678353
34-35	0.0012865583393079354
36-37	2.5751608992719376E-5
38-39	0.0017268211747486551
40-41	4.3896468018114434E-4
42-43	1.455175533525227E-4
44-45	1.7389703797162214E-4
46-47	6.77308951294781E-5
48-49	0.0
50-51	0.0
52-53	4.209340906311787E-5
54-55	3.4767906837903577E-4
56-57	6.39350178845641E-4
58-59	6.40387166437425E-4
60-61	6.045839109751665E-4
62-63	8.214160795035182E-5
64-65	1.2099387392892063E-4
66-67	1.9928864684005238E-4
68-69	9.331176276417435E-5
70-71	1.4203302441413547E-4
72-73	0.0
74-75	0.0
76-77	7.332785104658205E-4
78-79	1.672340779570016E-5
80-81	1.444263779317174E-4
82-83	8.632904470782668E-6
84-85	4.3846714342076105E-5
86-87	3.565087808112714E-5
88-89	3.627836242374288E-5
90-91	5.5455689102097524E-5
92-93	6.601683467007993E-5
94-95	3.084526434584327E-4
96-97	4.9498775400296596E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	310.0
20-21	899.0
22-23	1720.0
24-25	4090.0
26-27	5965.0
28-29	11230.0
30-31	23266.0
32-33	29620.0
34-35	39762.0
36-37	60644.0
38-39	70801.0
40-41	70970.0
42-43	79849.0
44-45	92541.0
46-47	91696.0
48-49	86891.0
50-51	80878.0
52-53	78660.0
54-55	85130.0
56-57	89300.0
58-59	89161.0
60-61	87395.0
62-63	83538.0
64-65	86826.0
66-67	93016.0
68-69	93344.0
70-71	92404.0
72-73	87807.0
74-75	89715.0
76-77	87171.0
78-79	93736.0
80-81	94591.0
82-83	90192.0
84-85	90857.0
86-87	95699.0
88-89	101649.0
90-91	106871.0
92-93	112297.0
94-95	126786.0
96-97	186781.0
98-99	643784.0
100-101	4259543.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.27285987415723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.1396278888731	79.54015973728455
2	6.330208468620004	11.04910793312154
3	1.452675086042935	3.8033732768071307
4	0.5043567758047165	1.7606663288554703
5	0.2358097012651741	1.0289893507741208
6	0.11340151054824942	0.5938124483757081
7	0.06454670760351666	0.39432230376139366
8	0.03937161162457921	0.27488585154653133
9	0.028776159282699672	0.22602399451159297
>10	0.08928350882640103	1.1617981993134454
>50	0.0015795087004783158	0.08694575224310254
>100	3.201532965490358E-4	0.05300193069451589
>500	4.291951142706819E-5	0.026912892710931575
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.0649677076652586E-5	0.0	0.0	0.0
5	0.0	6.331209634581573E-5	0.0	2.5324838538326293E-5	0.0
6	3.798725780748944E-5	6.331209634581573E-5	0.0	5.0649677076652586E-5	0.0
7	3.798725780748944E-5	7.597451561497888E-5	0.0	5.0649677076652586E-5	0.0
8	3.798725780748944E-5	7.597451561497888E-5	0.0	5.0649677076652586E-5	0.0
9	3.798725780748944E-5	2.152611275757735E-4	0.0	5.0649677076652586E-5	0.0
10-11	5.0649677076652586E-5	2.152611275757735E-4	0.0	8.863693488414203E-5	0.0
12-13	5.0649677076652586E-5	2.152611275757735E-4	0.0	9.496814451872361E-5	0.0
14-15	5.0649677076652586E-5	3.2922290099824184E-4	0.0	1.0129935415330517E-4	0.0
16-17	8.230572524956046E-5	3.2922290099824184E-4	0.0	1.0129935415330517E-4	0.0
18-19	8.863693488414203E-5	3.41885320267405E-4	0.0	1.2662419269163146E-4	0.0
20-21	8.863693488414203E-5	3.5454773953656814E-4	0.0	1.5194903122995777E-4	0.0
22-23	9.496814451872361E-5	4.1785983588238384E-4	0.0	2.0259870830661035E-4	6.331209634581573E-6
24-25	1.0763056378788675E-4	4.368534647861286E-4	0.0	2.0259870830661035E-4	1.2662419269163147E-5
26-27	1.1396177342246832E-4	4.4318467442071015E-4	0.0	3.0389806245991553E-4	1.2662419269163147E-5
28-29	1.1396177342246832E-4	4.4318467442071015E-4	0.0	7.090954790731363E-4	1.2662419269163147E-5
30-31	1.1396177342246832E-4	5.128279804011075E-4	0.0	0.0020386495023352667	1.899362890374472E-5
32-33	1.1396177342246832E-4	5.444840285740154E-4	0.0	0.004457171582745428	3.798725780748944E-5
34-35	1.2029298305704989E-4	6.077961249198311E-4	0.0	0.007920343252861548	3.798725780748944E-5
36-37	1.2662419269163146E-4	6.584458019964837E-4	0.0	0.012725731365508964	3.798725780748944E-5
38-39	1.2662419269163146E-4	6.8377064053481E-4	0.0	0.020373832604083503	3.798725780748944E-5
40-41	1.2662419269163146E-4	7.344203176114626E-4	0.0	0.028427131259271265	3.798725780748944E-5
42-43	1.2662419269163146E-4	7.534139465152072E-4	0.0	0.03565104145232884	4.431846744207102E-5
44-45	1.3295540232621304E-4	8.230572524956046E-4	0.0	0.044039894218149425	5.0649677076652586E-5
46-47	1.3928661196079461E-4	8.800381392068387E-4	0.0	0.0532581354461002	5.6980886711234155E-5
48-49	1.3928661196079461E-4	8.863693488414203E-4	0.0	0.061646988211920786	6.331209634581573E-5
50-51	1.5194903122995777E-4	9.876687029947255E-4	0.0	0.06955466904551316	6.331209634581573E-5
52-53	1.5194903122995777E-4	9.93999912629307E-4	0.0	0.07746868108874014	6.964330598039731E-5
54-55	1.5194903122995777E-4	0.0010129935415330517	0.0	0.08540168676087084	7.597451561497888E-5
56-57	1.5194903122995777E-4	0.001025655960802215	0.0	0.09384118920376808	7.597451561497888E-5
58-59	1.5194903122995777E-4	0.0010763056378788674	0.0	0.10237565979118404	7.597451561497888E-5
60-61	1.5194903122995777E-4	0.0011269553149555202	0.0	0.11037197755966058	7.597451561497888E-5
62-63	1.7727386976828407E-4	0.001183936201666754	0.0	0.11752624444673775	7.597451561497888E-5
64-65	1.7727386976828407E-4	0.0012535795076471516	0.0	0.12497174697700567	7.597451561497888E-5
66-67	1.7727386976828407E-4	0.001259910717281733	0.0	0.13284777176242515	7.597451561497888E-5
68-69	1.7727386976828407E-4	0.0012789043461854778	0.0	0.14064149082259508	7.597451561497888E-5
70-71	1.7727386976828407E-4	0.0013105603943583857	0.0	0.1477071207747881	7.597451561497888E-5
72-73	1.7727386976828407E-4	0.0013295540232621304	0.0	0.15353816484823773	7.597451561497888E-5
74-75	1.7727386976828407E-4	0.0013612100714350383	0.0	0.1587360879582292	7.597451561497888E-5
76-77	1.7727386976828407E-4	0.001411859748511691	0.0	0.16325657163732046	7.597451561497888E-5
78-79	1.7727386976828407E-4	0.0014181909581462726	0.0	0.16696032927355067	7.597451561497888E-5
80-81	1.7727386976828407E-4	0.0014371845870500173	0.0	0.16856845652073438	8.230572524956046E-5
82-83	1.7727386976828407E-4	0.0014435157966845987	0.0	0.16922690232273088	8.863693488414203E-5
84-85	1.7727386976828407E-4	0.0014625094255883435	0.0	0.17008161562339938	9.496814451872361E-5
86-87	1.7727386976828407E-4	0.0014751718448575068	0.0	0.17055012513635842	1.0129935415330517E-4
88-89	1.7727386976828407E-4	0.0015258215219341592	0.0	0.1707020741675884	1.0129935415330517E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7910	0.0	42.712578	1
GTATCAA	13860	0.0	33.32128	1
TCAACGC	16145	0.0	27.867945	4
ATCAACG	16335	0.0	27.543972	3
CAACGCA	16605	0.0	27.07121	5
AACGCAG	17130	0.0	26.313593	6
TATCAAC	17805	0.0	25.339083	2
ACGCAGA	20185	0.0	22.249678	7
GTACATG	19850	0.0	22.211412	1
CGCAGAG	20480	0.0	21.92919	8
TACATGG	19895	0.0	21.542284	2
ACATGGG	20015	0.0	20.879848	3
CATGGGG	14780	0.0	18.970497	4
GCAGAGT	25025	0.0	17.913523	9
TATACCG	455	0.0	17.142519	5
AGAGTAC	21835	0.0	17.127642	10-11
GAGTACT	14815	0.0	16.542809	12-13
CAGAGTA	24250	0.0	16.149872	10-11
ATGGGGG	9705	0.0	15.143279	5
AGTACTT	16290	0.0	14.629105	12-13
>>END_MODULE