FastQCFastQC Report
Fri 27 May 2016
SRR2139947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398305
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.046.2817151
GTATCAA196800.033.665651
TCAACGC232450.027.6950244
ATCAACG233450.027.558523
CAACGCA236100.027.3023365
AACGCAG246100.026.260986
TATCAAC253900.025.536162
ACGCAGA291350.022.1537067
CGCAGAG293750.021.9300948
GTACATG264100.020.6317631
TACATGG263050.020.349592
ACATGGG261950.019.7978523
GCAGAGT357950.017.8567249
CATGGGG186700.017.6318574
AGAGTAC320200.017.46670210-11
GAGTACT230800.016.85700212-13
CAGAGTA349950.016.31564310-11
AGTACTT257200.015.37817112-13
GAGTACA193650.015.0598711
ATGGGGG126350.014.69440655
Produced by FastQC (version 0.11.5)