##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11398305
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476897749270616	31.0	31.0	34.0	30.0	34.0
2	31.67932933887977	31.0	31.0	34.0	30.0	34.0
3	31.733676893187187	31.0	31.0	34.0	30.0	34.0
4	35.49137911294706	37.0	35.0	37.0	33.0	37.0
5	35.205637680339315	37.0	35.0	37.0	32.0	37.0
6	35.22676520763394	37.0	35.0	37.0	32.0	37.0
7	35.188064716639886	37.0	35.0	37.0	32.0	37.0
8	35.17270067786394	37.0	35.0	37.0	32.0	37.0
9	36.90602593982175	39.0	37.0	39.0	33.0	39.0
10-11	36.72360175482232	38.5	36.0	39.0	32.0	39.0
12-13	36.73114511324272	38.5	36.0	39.0	32.0	39.0
14-15	37.77108995591888	39.0	37.0	40.0	33.0	41.0
16-17	37.689299242299626	39.0	37.0	40.0	32.5	41.0
18-19	37.753396667311506	39.0	37.0	40.5	33.0	41.0
20-21	37.80078926240871	39.0	37.0	40.5	33.0	41.0
22-23	37.782852057895084	39.0	37.0	40.0	33.0	41.0
24-25	37.73366324957491	39.0	37.0	41.0	32.0	41.0
26-27	37.59572625728291	39.0	37.0	40.5	32.0	41.0
28-29	37.41617815946363	39.0	36.5	40.0	32.0	41.0
30-31	37.30760315258959	39.0	36.0	40.0	32.0	41.0
32-33	37.201694646300936	39.0	36.0	40.0	31.0	41.0
34-35	37.12480530314997	39.0	36.0	40.0	31.0	41.0
36-37	37.0768277742083	39.0	36.0	40.0	31.0	41.0
38-39	36.99094803443667	39.0	36.0	40.0	31.0	41.0
40-41	36.97810232428425	39.0	36.0	40.0	31.0	41.0
42-43	36.92716495384444	39.0	35.5	40.0	31.0	41.0
44-45	36.85965125489241	39.0	35.0	40.0	30.5	41.0
46-47	36.80889265003443	39.0	35.0	40.0	30.0	41.0
48-49	36.82960391739071	39.0	35.0	40.0	30.0	41.0
50-51	36.73023315386499	39.0	35.0	40.0	30.0	41.0
52-53	36.5489065353582	38.0	35.0	40.0	30.0	41.0
54-55	36.361644271423394	38.0	35.0	40.0	30.0	41.0
56-57	36.25167812955578	38.0	35.0	40.0	30.0	41.0
58-59	36.07453566492589	38.0	34.0	40.0	29.0	41.0
60-61	35.914957761248886	38.0	34.0	40.0	29.0	41.0
62-63	35.794052318841096	38.0	34.0	40.0	28.5	41.0
64-65	35.560655067496874	37.0	34.0	40.0	28.0	41.0
66-67	35.44725714222949	37.0	34.0	39.5	28.0	41.0
68-69	35.19955461940397	36.5	34.0	39.0	28.0	40.5
70-71	34.90018879025466	36.0	33.0	39.0	28.0	40.0
72-73	34.62025768384918	36.0	33.0	39.0	28.0	40.0
74-75	34.27920690499657	35.0	32.5	38.0	28.0	40.0
76-77	32.1556954088749	33.5	30.0	35.5	25.5	38.5
78-79	33.44593184547803	35.0	32.0	37.0	26.5	39.0
80-81	33.467371056956466	35.0	32.0	36.5	27.0	39.0
82-83	33.30256832031163	35.0	32.0	36.0	27.0	38.5
84-85	33.09003354479397	34.5	32.0	36.0	27.0	37.0
86-87	32.83315180801387	34.0	32.0	35.0	27.0	37.0
88-89	32.662823954101746	34.0	32.0	35.0	27.0	36.5
90-91	32.492261580852656	34.0	31.5	35.0	26.5	36.0
92-93	32.461584935788366	34.0	31.5	35.0	27.0	36.0
94-95	32.42872084899177	34.0	32.0	35.0	27.0	35.5
96-97	32.47421237632364	34.0	32.0	35.0	27.0	35.0
98-99	32.65237953062818	34.0	32.0	35.0	28.5	35.0
100-101	31.48371732782028	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	3.0
19	36.0
20	174.0
21	788.0
22	3086.0
23	9847.0
24	24045.0
25	49249.0
26	86403.0
27	134407.0
28	192306.0
29	259153.0
30	334042.0
31	424715.0
32	534883.0
33	687696.0
34	910665.0
35	1251077.0
36	1797117.0
37	2335472.0
38	1873060.0
39	487181.0
40	2898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43728431551884	21.65730781901344	14.05216828291575	23.853239582551964
2	14.818995761549408	22.694068722189307	40.46661822071404	22.020317295547244
3	17.948407510707533	27.163292168375825	31.269900468377397	23.618399852539245
4	12.336556795915744	18.898648237254985	39.832599627558565	28.93219533927071
5	12.295924701084942	38.645921476921345	36.71580116517325	12.342352656820466
6	28.82343471244189	39.76521947780832	19.020108691599322	12.391237118150462
7	25.371096842907782	33.11607295997081	24.431623824770437	17.081206372350977
8	23.74275213871676	37.63544036377706	21.915383056432646	16.70642444107354
9	25.128148439614485	16.36622287261132	21.805180682566398	36.70044800520779
10-11	23.37619331621847	27.287999117059613	30.151427259450948	19.184380307270978
12-13	24.155113034621834	24.884593085319064	30.097351300819675	20.86294257923943
14-15	21.241129382055433	26.404160654076254	27.49599852750023	24.85871143636808
16-17	20.951466948439567	29.50453727569745	28.198321074858107	21.345674701004874
18-19	20.93352906509134	28.85508546156527	29.71644118254092	20.494944290802472
20-21	21.686134991469487	28.069061632315844	29.60400536375303	20.64079801246164
22-23	21.33707488631299	28.05904116547755	29.42615032439945	21.177733623810006
24-25	21.166641701213425	28.147623204731186	29.52522905711913	21.160506036936262
26-27	21.180278759812605	28.31225209140118	29.387590836696596	21.119878312089618
28-29	21.15380863082933	28.4139529209593	29.370347010696072	21.061891437515293
30-31	21.436638960417778	28.273282295529594	29.291842221070013	20.998236522982612
32-33	21.202664098505096	28.39743126783183	29.23062492965872	21.16927970400435
34-35	21.3213207099496	28.426450756096973	29.235252361529234	21.016976172424194
36-37	21.298258276672613	28.380477450590142	29.18251661852419	21.138747654213052
38-39	21.155705161903825	28.490198253721104	29.261281163325503	21.09281542104957
40-41	21.40379189410596	28.39307929376324	29.104460656240537	21.098668155890262
42-43	21.277390670741372	28.498215274988866	28.939133973212112	21.285260081057654
44-45	21.413442969732817	28.59037034662472	28.742586562166668	21.253600121475795
46-47	21.410274839279634	28.55548515284375	28.559179634164767	21.47506037371185
48-49	21.375271593530154	28.65175776560046	28.584535702597975	21.38843493827141
50-51	21.361704266216424	28.768462309047578	28.43067705311373	21.43915637162227
52-53	21.517240720279514	28.62456236748589	28.368840445546034	21.489356466688566
54-55	21.50014587319754	28.574851190519272	28.438874980348377	21.48612795593481
56-57	21.48152187836489	28.704368102356366	28.356265845501365	21.457844173777378
58-59	21.46390676045514	28.74424471314894	28.348200849322353	21.443647677073567
60-61	21.547084215763405	28.694052743690097	28.35064316417215	21.40821987637435
62-63	21.501302359383736	28.726828270072215	28.320819518269662	21.45104985227439
64-65	21.492268438590596	28.679127268634435	28.31922820072578	21.509376092049184
66-67	21.573150575268816	28.723248437428992	28.163466644428357	21.54013434287383
68-69	21.54709826150616	28.70060313595491	28.21005682028334	21.542241782255587
70-71	21.519818628365062	28.509825226333476	28.281400511341225	21.68895563396024
72-73	21.573446128149875	28.510357494647465	28.287087544690596	21.62910883251206
74-75	21.452628200479644	28.628480728192248	28.248066238621927	21.670824832706185
76-77	21.536034970500555	28.582142634822482	28.18527171995933	21.696550674717628
78-79	21.533917659618474	28.59811742689197	28.183035385133852	21.684929528355703
80-81	21.552662041553333	28.505769467063057	28.217072527586705	21.7244959637969
82-83	21.5476006030298	28.527529347022035	28.305344640277035	21.619525409671134
84-85	21.669562893917945	28.48707895672838	28.24671696611843	21.596641183235242
86-87	21.534431916283218	28.470118012739086	28.299334456445298	21.696115614532403
88-89	21.561647994663847	28.480490414757657	28.292247067448677	21.66561452312982
90-91	21.616686971459952	28.45357025673255	28.328965902816535	21.600776868990962
92-93	21.603839024318013	28.382094188372946	28.344848524809752	21.66921826249929
94-95	21.453798363654265	28.414692913141025	28.458295982714223	21.673212740490488
96-97	21.4089803615572	28.435947731410234	28.503106903112446	21.651965003920118
98-99	21.627395078849585	29.060642465300262	28.519558565120974	20.79240389072918
100-101	22.358293391963144	31.73941349223487	25.746910426854118	20.15538268894787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1628.0
1	1557.5
2	2587.5
3	3966.5
4	5348.5
5	8712.0
6	11761.0
7	14060.5
8	15852.0
9	15888.5
10	14552.5
11	12903.0
12	13506.5
13	17585.0
14	27287.0
15	40840.0
16	51773.0
17	58289.5
18	61332.5
19	61519.0
20	60410.0
21	60084.5
22	62685.0
23	70577.5
24	84495.5
25	105495.0
26	133780.0
27	164057.0
28	194293.5
29	225633.5
30	254786.5
31	280659.5
32	306341.5
33	331556.5
34	356305.5
35	379842.5
36	405049.5
37	426493.0
38	440518.0
39	451811.0
40	463366.0
41	474034.0
42	477495.5
43	479986.0
44	482164.5
45	481342.0
46	477033.0
47	471960.5
48	465849.0
49	452422.0
50	436334.5
51	415346.5
52	388199.5
53	361758.5
54	335449.5
55	306692.0
56	276529.0
57	245976.5
58	214534.5
59	183479.0
60	153109.5
61	124019.0
62	97445.0
63	74679.5
64	56252.0
65	42328.5
66	32073.5
67	24470.5
68	18633.0
69	13465.5
70	9540.5
71	6913.5
72	4915.0
73	3345.0
74	2264.0
75	1573.5
76	1113.5
77	774.5
78	531.5
79	373.0
80	271.5
81	194.5
82	136.0
83	87.0
84	58.0
85	46.5
86	38.5
87	32.5
88	27.0
89	22.0
90	17.0
91	11.5
92	8.5
93	6.5
94	5.5
95	5.0
96	5.0
97	4.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.1583643357499205E-4
3	1.5791821678749602E-4
4	2.6319702797916007E-5
5	0.0
6	0.0
7	0.0
8	0.0010089219405867802
9	0.0
10-11	2.3687732518124408E-4
12-13	4.342750961656141E-4
14-15	8.773234265972002E-6
16-17	4.386617132986001E-6
18-19	4.605947989635301E-4
20-21	0.0
22-23	0.0
24-25	1.7555517898969664E-4
26-27	0.0
28-29	4.6582411096809234E-4
30-31	3.476857420854842E-4
32-33	9.617719050279362E-4
34-35	0.001044477803806617
36-37	4.446039288137536E-5
38-39	0.00166474020722167
40-41	3.471196484913166E-4
42-43	1.2267541448387727E-4
44-45	1.467539920181421E-4
46-47	2.3177418222883512E-5
48-49	9.368927763693155E-6
50-51	3.3125469435223995E-5
52-53	2.388986200260113E-5
54-55	2.991766850644243E-4
56-57	5.512084611376816E-4
58-59	4.933994988639969E-4
60-61	5.790348357839735E-4
62-63	1.1107745688566378E-4
64-65	5.6174352935288675E-5
66-67	1.4476247553966545E-4
68-69	1.2049502499616904E-4
70-71	7.435521942039363E-5
72-73	0.0
74-75	5.456552473755347E-6
76-77	6.695256703888604E-4
78-79	2.244444466888889E-5
80-81	1.3101856567254336E-4
82-83	5.7854570849546354E-6
84-85	0.0
86-87	5.971035105805249E-6
88-89	3.0385345123738546E-5
90-91	5.575967382325559E-5
92-93	3.1639187715743665E-5
94-95	2.4612486121472466E-4
96-97	3.998758252270728E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	438.0
20-21	1358.0
22-23	2656.0
24-25	5616.0
26-27	7711.0
28-29	13730.0
30-31	26010.0
32-33	33167.0
34-35	45668.0
36-37	68771.0
38-39	81451.0
40-41	84282.0
42-43	97008.0
44-45	114628.0
46-47	114736.0
48-49	109121.0
50-51	102715.0
52-53	100675.0
54-55	109565.0
56-57	115726.0
58-59	117409.0
60-61	115136.0
62-63	111180.0
64-65	117208.0
66-67	126358.0
68-69	129144.0
70-71	128777.0
72-73	123521.0
74-75	128564.0
76-77	122493.0
78-79	132255.0
80-81	135728.0
82-83	131901.0
84-85	134717.0
86-87	143703.0
88-89	153959.0
90-91	167009.0
92-93	175905.0
94-95	203383.0
96-97	295384.0
98-99	968971.0
100-101	6300568.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19468503120969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.71149079827111	76.42942202238973
2	7.107574334699248	12.110551135612246
3	1.8002693931080282	4.601201517514965
4	0.6588760219656603	2.245309406639233
5	0.2880386633723577	1.2269681601409324
6	0.15285786124745276	0.7813606406112654
7	0.08516205180976295	0.5078747926381022
8	0.05404763983004721	0.3683657321600909
9	0.03494030907738161	0.26790557640665785
>10	0.105271007203006	1.3230275829070093
>50	0.0011272224963597244	0.06403068346896658
>100	2.9668896652654606E-4	0.04354158636435749
>500	4.800795306523075E-5	0.030441163146434958
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.773234265972002E-6
2	0.0	8.773234265972002E-6	0.0	0.0	8.773234265972002E-6
3	0.0	8.773234265972002E-6	0.0	0.0	8.773234265972002E-6
4	0.0	4.386617132986001E-5	0.0	8.773234265972002E-6	8.773234265972002E-6
5	0.0	4.386617132986001E-5	0.0	1.7546468531944004E-5	8.773234265972002E-6
6	0.0	4.386617132986001E-5	0.0	1.7546468531944004E-5	3.509293706388801E-5
7	0.0	4.386617132986001E-5	0.0	2.6319702797916007E-5	3.509293706388801E-5
8	0.0	4.386617132986001E-5	0.0	2.6319702797916007E-5	3.509293706388801E-5
9	0.0	6.141263986180402E-5	0.0	7.018587412777602E-5	4.386617132986001E-5
10-11	0.0	6.141263986180402E-5	0.0	8.773234265972002E-5	4.386617132986001E-5
12-13	0.0	6.579925699479002E-5	0.0	9.211895979270602E-5	4.386617132986001E-5
14-15	0.0	1.0089219405867803E-4	0.0	1.0089219405867803E-4	5.702602272881802E-5
16-17	0.0	1.1405204545763603E-4	0.0	1.0527881119166403E-4	7.895910839374801E-5
18-19	0.0	1.2282527972360804E-4	0.0	1.2282527972360804E-4	8.773234265972002E-5
20-21	0.0	1.3159851398958003E-4	0.0	2.4565055944721607E-4	9.211895979270602E-5
22-23	0.0	1.4914498252152403E-4	0.0	3.6408922203783804E-4	1.0966542832465003E-4
24-25	0.0	1.7985130245242603E-4	0.0	5.132342045593621E-4	1.2282527972360804E-4
26-27	0.0	2.1055762238332806E-4	0.0	8.553903409322702E-4	1.2282527972360804E-4
28-29	0.0	2.1055762238332806E-4	0.0	0.0029521933304995788	1.2282527972360804E-4
30-31	0.0	2.2810409091527205E-4	0.0	0.006781710087596357	1.2282527972360804E-4
32-33	0.0	2.2810409091527205E-4	0.0	0.012953680393707662	1.3159851398958003E-4
34-35	0.0	2.7197026224513204E-4	0.0	0.022349814292563674	1.4914498252152403E-4
36-37	0.0	3.2899628497395006E-4	0.0	0.03515434970374981	1.4914498252152403E-4
38-39	0.0	3.6408922203783804E-4	0.0	0.05127955428460636	1.5353159965451E-4
40-41	0.0	3.947955419687401E-4	0.0	0.06855405255430522	1.5791821678749602E-4
42-43	0.0	4.035687762347121E-4	0.0	0.08106468461758129	1.7107806818645403E-4
44-45	0.0	4.605947989635301E-4	0.0	0.0966678817596125	1.8862453671839805E-4
46-47	0.0	5.088475874263761E-4	0.0	0.11120074432119512	1.9739777098437002E-4
48-49	0.0	5.088475874263761E-4	0.0	0.12284721280927297	2.1933085664930006E-4
50-51	0.0	5.702602272881802E-4	0.0	0.1343971757204251	2.1933085664930006E-4
52-53	0.0	5.790334615541522E-4	0.0	0.14513122784484184	2.2371747378228604E-4
54-55	0.0	5.878066958201241E-4	0.0	0.15593546584338636	2.3687732518124405E-4
56-57	0.0	6.053531643520682E-4	0.0	0.16609048450624897	2.5003717658020206E-4
58-59	0.0	6.053531643520682E-4	0.0	0.17609197156945705	2.588104108461741E-4
60-61	4.386617132986001E-6	6.185130157510261E-4	0.0	0.18529509431446167	2.76356879378118E-4
62-63	8.773234265972002E-6	6.228996328840121E-4	0.0	0.19441048471680655	2.9828996504304805E-4
64-65	8.773234265972002E-6	6.667658042138722E-4	0.0	0.2026090721383574	2.9828996504304805E-4
66-67	8.773234265972002E-6	6.755390384798442E-4	0.0	0.21143055919279227	2.9828996504304805E-4
68-69	8.773234265972002E-6	6.886988898788021E-4	0.0	0.22015115405316843	3.0267658217603404E-4
70-71	8.773234265972002E-6	7.018587412777602E-4	0.0	0.22776193477889914	3.202230507079781E-4
72-73	8.773234265972002E-6	7.150185926767181E-4	0.0	0.23307412812694517	3.684758391708241E-4
74-75	8.773234265972002E-6	7.237918269426902E-4	0.0	0.23756602407112287	3.684758391708241E-4
76-77	8.773234265972002E-6	7.369516783416482E-4	0.0	0.2411893698229693	3.772490734367961E-4
78-79	8.773234265972002E-6	7.369516783416482E-4	0.0	0.24451881222690566	3.860223077027681E-4
80-81	8.773234265972002E-6	7.413382954746341E-4	0.0	0.24627784569723304	3.9479554196874005E-4
82-83	8.773234265972002E-6	7.457249126076201E-4	0.0	0.24710252971823443	4.211152447666561E-4
84-85	8.773234265972002E-6	7.457249126076201E-4	0.0	0.24787018771650698	4.211152447666561E-4
86-87	8.773234265972002E-6	7.544981468735922E-4	0.0	0.24816409106441706	4.211152447666561E-4
88-89	8.773234265972002E-6	7.808178496715082E-4	0.0	0.2483000761955396	4.211152447666561E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11025	0.0	46.281715	1
GTATCAA	19680	0.0	33.66565	1
TCAACGC	23245	0.0	27.695024	4
ATCAACG	23345	0.0	27.55852	3
CAACGCA	23610	0.0	27.302336	5
AACGCAG	24610	0.0	26.26098	6
TATCAAC	25390	0.0	25.53616	2
ACGCAGA	29135	0.0	22.153706	7
CGCAGAG	29375	0.0	21.930094	8
GTACATG	26410	0.0	20.631763	1
TACATGG	26305	0.0	20.34959	2
ACATGGG	26195	0.0	19.797852	3
GCAGAGT	35795	0.0	17.856724	9
CATGGGG	18670	0.0	17.631857	4
AGAGTAC	32020	0.0	17.466702	10-11
GAGTACT	23080	0.0	16.857002	12-13
CAGAGTA	34995	0.0	16.315643	10-11
AGTACTT	25720	0.0	15.378171	12-13
GAGTACA	19365	0.0	15.059871	1
ATGGGGG	12635	0.0	14.6944065	5
>>END_MODULE