FastQCFastQC Report
Fri 27 May 2016
SRR2139946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18961539
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190250.047.702911
GTATCAA334700.035.392821
TCAACGC398150.028.8142914
ATCAACG401800.028.5316433
CAACGCA411250.027.8863755
AACGCAG423900.027.1237266
TATCAAC435800.026.6236822
ACGCAGA496650.023.13377
CGCAGAG505800.022.7152088
GTACATG477100.021.8552651
TACATGG477850.021.3906842
ACATGGG476700.020.8345723
CATGGGG351100.018.6751984
GCAGAGT619350.018.4691839
GAGTACT378500.017.65042712-13
AGAGTAC554050.017.44866810-11
CAGAGTA598200.016.88365610-11
GTGGTAT105100.015.9761131
AGTACTT415050.015.69660512-13
GTACTTT435250.015.40759514-15
Produced by FastQC (version 0.11.5)