##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18961539
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85242785408927	31.0	31.0	34.0	30.0	34.0
2	32.033886542648254	33.0	31.0	34.0	30.0	34.0
3	32.09890104384459	33.0	31.0	34.0	30.0	34.0
4	35.7867740060551	37.0	35.0	37.0	35.0	37.0
5	35.55225564760329	37.0	35.0	37.0	33.0	37.0
6	35.58260154937845	37.0	35.0	37.0	33.0	37.0
7	35.542195862899106	37.0	35.0	37.0	33.0	37.0
8	35.53710155067054	37.0	35.0	37.0	33.0	37.0
9	37.319486619730604	39.0	37.0	39.0	34.0	39.0
10-11	37.17049546980337	39.0	37.0	39.0	33.0	39.0
12-13	37.17600129926163	39.0	37.0	39.0	33.0	39.0
14-15	38.308593516591664	40.0	38.0	41.0	33.5	41.0
16-17	38.24431363403572	40.0	38.0	41.0	33.0	41.0
18-19	38.31006707841594	40.0	38.0	41.0	33.5	41.0
20-21	38.34942189535411	40.0	38.0	41.0	34.0	41.0
22-23	38.3346261406619	40.0	38.0	41.0	34.0	41.0
24-25	38.290814956107084	40.0	38.0	41.0	34.0	41.0
26-27	38.177121311802864	40.0	38.0	41.0	34.0	41.0
28-29	38.010223638572164	40.0	37.0	41.0	33.0	41.0
30-31	37.91468406509074	40.0	37.0	41.0	33.0	41.0
32-33	37.82446932021317	40.0	37.0	41.0	33.0	41.0
34-35	37.75768258226603	40.0	37.0	41.0	33.0	41.0
36-37	37.71709367446887	40.0	37.0	41.0	33.0	41.0
38-39	37.63464585314638	39.0	37.0	41.0	33.0	41.0
40-41	37.62214318415998	39.0	37.0	41.0	33.0	41.0
42-43	37.57202948468196	39.0	37.0	41.0	32.5	41.0
44-45	37.50464471200877	39.0	37.0	41.0	32.0	41.0
46-47	37.45582446874177	39.0	36.5	41.0	32.0	41.0
48-49	37.47449613003994	39.0	36.5	41.0	32.0	41.0
50-51	37.37509674555939	39.0	36.0	40.5	31.5	41.0
52-53	37.19431793065951	39.0	36.0	40.0	31.0	41.0
54-55	37.00891851410894	39.0	35.5	40.0	31.0	41.0
56-57	36.897969123830954	39.0	35.0	40.0	31.0	41.0
58-59	36.71692038715606	38.5	35.0	40.0	31.0	41.0
60-61	36.54595524006392	38.0	35.0	40.0	30.5	41.0
62-63	36.40932423939929	38.0	35.0	40.0	30.5	41.0
64-65	36.15598395651624	38.0	34.0	40.0	30.0	41.0
66-67	36.02080125680981	37.0	34.0	40.0	30.0	41.0
68-69	35.75341647232277	37.0	34.0	39.5	30.0	41.0
70-71	35.437044074324106	36.0	34.0	39.0	29.0	41.0
72-73	35.14396607808248	36.0	34.0	39.0	29.0	40.0
74-75	34.79183186983197	35.5	33.5	38.5	29.0	40.0
76-77	32.64713093779307	33.5	30.5	36.0	26.5	38.5
78-79	33.92047655870015	35.0	32.5	37.0	28.5	39.0
80-81	33.92028197765865	35.0	33.0	36.5	29.0	39.0
82-83	33.7451109671317	35.0	33.0	36.0	29.0	38.5
84-85	33.53803698887818	35.0	33.0	36.0	29.0	37.0
86-87	33.292237522125774	35.0	33.0	35.0	29.0	37.0
88-89	33.12686090233491	35.0	33.0	35.0	29.0	36.5
90-91	32.962543927661144	34.0	32.0	35.0	29.0	36.0
92-93	32.92003706641952	34.0	32.0	35.0	29.0	36.0
94-95	32.87699706862869	34.0	32.0	35.0	29.0	36.0
96-97	32.89711351234494	34.0	32.0	35.0	29.0	35.0
98-99	33.02930548578168	34.0	32.0	35.0	29.0	35.0
100-101	31.807660337490972	33.0	31.0	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	9.0
19	37.0
20	124.0
21	599.0
22	2697.0
23	9451.0
24	25316.0
25	53893.0
26	98576.0
27	161200.0
28	238016.0
29	329236.0
30	432286.0
31	557957.0
32	716006.0
33	940160.0
34	1279926.0
35	1817573.0
36	2808299.0
37	4205961.0
38	4002073.0
39	1269824.0
40	12317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6340783308781	20.54749880798178	13.467593532360429	24.350829328779692
2	14.913002711342848	22.019161717195406	40.26897094858711	22.79886462287463
3	18.01193333420176	26.63252451400619	30.848829684265073	24.506712467526974
4	12.356883305398714	18.15831174445405	39.38774056132686	30.097064388820378
5	12.617668461281992	38.32766624732656	36.32988526563615	12.724780025755294
6	29.63142917882351	38.76668977133132	18.86231913981244	12.739561910032723
7	25.982389931534566	32.814324828802135	23.97291169245281	17.230373547210487
8	24.342034898377435	36.86910840689529	21.705111558571282	17.08374513615599
9	25.608232538508606	16.13606363913815	21.367674849599496	36.88802897275374
10-11	23.821001599269845	26.83664667164044	29.782370988149626	19.55998074094009
12-13	24.619006901127975	24.449684545743576	29.68453572325409	21.24677282987436
14-15	21.695486937821183	25.89564728346403	27.06280998935846	25.346055789356324
16-17	21.44213987283099	28.96814066453892	27.760411629056676	21.829307833573417
18-19	21.467802313887848	28.33367793577748	29.277167872617007	20.92135187771767
20-21	22.174305437510643	27.59114155704378	29.140190837462214	21.09436216798336
22-23	21.845747798127036	27.537100607682152	28.97752143336747	21.639630160823344
24-25	21.674567385394116	27.617987384457066	29.065025572559843	21.642419657588974
26-27	21.64582200901828	27.775897265153997	28.97201414514997	21.60626658067775
28-29	21.644320054710118	27.879112619448904	28.927348098003275	21.549219227837707
30-31	21.90578707024611	27.731395520085293	28.83124119263794	21.531576217030654
32-33	21.641294465804076	27.91864993738925	28.795118179033448	21.644937417773228
34-35	21.79158319877556	27.911777281492522	28.798641763932487	21.49799775579943
36-37	21.766467325471016	27.826987749219313	28.782376619767643	21.62416830554203
38-39	21.63241096928081	28.008812131347	28.809318287905818	21.549458611466374
40-41	21.896563870413956	27.878738876359144	28.636758780192324	21.587938473034576
42-43	21.762493655557513	27.996909198530854	28.475131565864558	21.765465580047074
44-45	21.867573833246144	28.054241508711424	28.311006750599375	21.76717790744306
46-47	21.907673245632733	28.065104073114185	28.116644751068677	21.91057793018441
48-49	21.82142406401836	28.15226505142934	28.12903236378654	21.897278520765763
50-51	21.826717578097725	28.24862487573353	28.03463216112496	21.89002538504378
52-53	22.052716987243954	28.11812355444169	27.912324087980718	21.916835370333636
54-55	21.957667190084138	28.041553816824887	27.999604457537984	22.001174535552984
56-57	21.92730831295671	28.168773033802875	27.962158794361724	21.941759858878687
58-59	21.93743352702413	28.216209060019974	27.9086657528558	21.937691660100096
60-61	21.98188449428046	28.197011462103	27.9352508243589	21.88585321925764
62-63	21.984844502780778	28.20313062129965	27.908273265505983	21.90375161041359
64-65	21.9571517309547	28.17342000940687	27.89628521669467	21.973143042943757
66-67	21.99932264507778	28.211817040160724	27.766954015484487	22.021906299277013
68-69	21.97335008874758	28.21784200561357	27.836659879292274	21.972148026346577
70-71	21.97832882117111	27.965822557330146	27.924408968426185	22.131439653072558
72-73	22.012290723501877	27.998669682164905	27.883942112770566	22.105097481562648
74-75	21.854899464847453	28.11942400763108	27.865610430249205	22.16006609727226
76-77	21.985140426319802	28.050606723247988	27.799805457242776	22.164447393189427
78-79	21.978891565441934	28.069787820746217	27.763665442972446	22.187655170839406
80-81	21.97856200352268	28.048192399541037	27.78256396665688	22.1906816302794
82-83	22.021565511749216	28.01445184140573	27.868331897904852	22.095650748940198
84-85	22.112771314049525	27.983250640463257	27.819104815140104	22.084873230347114
86-87	21.925776451055903	27.97887081743785	27.91660493877136	22.178747792734885
88-89	21.99760758699904	27.97043748987792	27.872038309341086	22.159916613781956
90-91	22.061391233164223	27.942950545585532	27.90450718569806	22.09115103555219
92-93	22.083550138773877	27.843142579131502	27.95300783928736	22.12029944280726
94-95	21.948006873382006	27.83317611246564	28.02755759416926	22.19125941998309
96-97	21.927669424544543	27.83319830488187	28.08550828217811	22.153623988395477
98-99	22.18432872965676	28.44152660395477	28.133076586609285	21.241068079779186
100-101	22.914891230186683	31.083348811890477	25.272710721398845	20.729049236524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2802.0
1	2666.0
2	4285.5
3	6497.0
4	8885.0
5	14657.0
6	19894.0
7	23709.5
8	26422.5
9	26171.0
10	23695.5
11	21007.5
12	22240.0
13	28914.0
14	44385.5
15	65598.5
16	83000.0
17	93321.5
18	96881.0
19	95239.5
20	92132.0
21	90328.5
22	92701.0
23	104228.5
24	126327.5
25	157992.0
26	198812.5
27	242518.0
28	286415.0
29	331271.0
30	370336.0
31	408602.0
32	452022.0
33	494873.0
34	536076.5
35	573354.0
36	613409.0
37	650006.5
38	678131.5
39	702877.0
40	725173.5
41	745403.5
42	757066.5
43	766386.0
44	777442.0
45	785717.0
46	787672.0
47	786058.0
48	780909.5
49	768640.5
50	751540.5
51	728169.5
52	696297.0
53	661855.0
54	624545.5
55	579453.0
56	531402.0
57	480522.5
58	426780.0
59	371845.0
60	314832.5
61	259333.0
62	207169.5
63	160200.5
64	120323.5
65	89880.0
66	67698.0
67	51686.0
68	39564.0
69	28586.5
70	20044.5
71	14211.5
72	9837.5
73	6711.5
74	4581.5
75	3187.0
76	2244.0
77	1517.5
78	1043.0
79	727.0
80	477.5
81	299.5
82	225.0
83	173.0
84	129.5
85	103.0
86	87.0
87	69.5
88	48.5
89	33.0
90	20.5
91	13.0
92	11.5
93	8.5
94	5.5
95	4.5
96	4.0
97	3.5
98	2.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.535496828606581E-4
3	1.687626726923379E-4
4	1.0547667043271119E-5
5	5.2738335216355595E-6
6	0.0
7	0.0
8	0.0010494928708054763
9	0.0
10-11	3.876267638402136E-4
12-13	3.876267638402136E-4
14-15	5.2738335216355595E-6
16-17	5.2738335216355595E-6
18-19	3.981744308834847E-4
20-21	0.0
22-23	0.0
24-25	1.6883728096000878E-4
26-27	0.0
28-29	4.939523590081669E-4
30-31	4.127658748035592E-4
32-33	0.001095792189830051
34-35	0.001320664398567569
36-37	4.014226096146656E-5
38-39	0.001735893647079398
40-41	4.4556445341127816E-4
42-43	1.1778120049337723E-4
44-45	2.350539494442094E-4
46-47	5.0321747468529544E-5
48-49	2.8253298049861306E-6
50-51	2.853502440258217E-6
52-53	3.4558608808643795E-5
54-55	3.3136163471739796E-4
56-57	5.842829815778512E-4
58-59	5.578016963936399E-4
60-61	5.8471208192374E-4
62-63	1.333015469735414E-4
64-65	6.733179461819413E-5
66-67	2.0420853998876604E-4
68-69	1.533879356819969E-4
70-71	1.71090962791581E-4
72-73	0.0
74-75	1.6218117330229316E-5
76-77	6.861147028176236E-4
78-79	3.655230911237768E-5
80-81	1.6496333050325835E-4
82-83	1.0235594032594088E-5
84-85	3.802711326261604E-5
86-87	1.752388487985239E-5
88-89	4.979893147855553E-5
90-91	6.507241185688755E-5
92-93	6.254883638376037E-5
94-95	2.775691737077026E-4
96-97	6.912386916710231E-5
98-99	4.022141729334316E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	661.0
20-21	1845.0
22-23	3656.0
24-25	8436.0
26-27	12706.0
28-29	25852.0
30-31	49455.0
32-33	61702.0
34-35	84366.0
36-37	126147.0
38-39	147018.0
40-41	146380.0
42-43	165415.0
44-45	193640.0
46-47	191884.0
48-49	178403.0
50-51	163534.0
52-53	157107.0
54-55	169820.0
56-57	177112.0
58-59	177410.0
60-61	173406.0
62-63	166393.0
64-65	173397.0
66-67	185719.0
68-69	191290.0
70-71	188957.0
72-73	179532.0
74-75	185864.0
76-77	179227.0
78-79	193725.0
80-81	198439.0
82-83	191521.0
84-85	195102.0
86-87	205940.0
88-89	221094.0
90-91	239236.0
92-93	250971.0
94-95	288876.0
96-97	433710.0
98-99	1617698.0
100-101	1.1058893E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.67339388093329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.23630009986225	74.6670408673294
2	7.276291946548808	12.176640840724822
3	1.8774010154083236	4.712655439041743
4	0.7159876973878189	2.3963648246973377
5	0.3329580467261868	1.3929864894773205
6	0.18585939554702724	0.9330891846047151
7	0.10327469227045158	0.604894080299437
8	0.06744345883369088	0.45145784765471414
9	0.04870893566598614	0.3668077763550988
>10	0.1517763756249015	1.9596528682040217
>50	0.0032203390341727736	0.1838680057152347
>100	7.211728860290427E-4	0.10193687739590343
>500	3.409605211674495E-5	0.026570246599322896
>1k	2.27281523008345E-5	0.02603465190096392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.2738335216355595E-6
2	0.0	3.1643001129813354E-5	0.0	5.2738335216355595E-6	5.2738335216355595E-6
3	0.0	3.1643001129813354E-5	0.0	1.5821500564906677E-5	5.2738335216355595E-6
4	0.0	5.801216873799115E-5	0.0	4.2190668173084476E-5	5.2738335216355595E-6
5	0.0	6.328600225962671E-5	0.0	4.7464501694720034E-5	5.2738335216355595E-6
6	1.0547667043271119E-5	6.328600225962671E-5	0.0	4.7464501694720034E-5	3.691683465144891E-5
7	1.0547667043271119E-5	6.855983578126226E-5	0.0	5.801216873799115E-5	3.691683465144891E-5
8	2.1095334086542238E-5	7.383366930289782E-5	0.0	7.383366930289782E-5	4.2190668173084476E-5
9	2.1095334086542238E-5	1.3184583804088897E-4	0.0	1.160243374759823E-4	4.7464501694720034E-5
10-11	2.9006084368995575E-5	1.3184583804088897E-4	0.0	1.423935050841601E-4	5.801216873799115E-5
12-13	4.2190668173084476E-5	1.3448275480170676E-4	0.0	1.7403650621397346E-4	6.855983578126226E-5
14-15	4.2190668173084476E-5	1.819472564964268E-4	0.0	2.0304259058296904E-4	1.1075050395434674E-4
16-17	6.064908549880893E-5	1.8458417325724458E-4	0.0	2.056795073437868E-4	1.3184583804088897E-4
18-19	6.855983578126226E-5	1.8985800677888013E-4	0.0	2.425963419952357E-4	1.3711967156252453E-4
20-21	6.855983578126226E-5	1.951318403005157E-4	0.0	2.8215009340750244E-4	1.4766733860579567E-4
22-23	7.91075028245334E-5	2.0040567382215125E-4	0.0	3.4016226214549355E-4	1.713995894531557E-4
24-25	8.438133634616895E-5	2.188640911478757E-4	0.0	3.876267638402136E-4	1.951318403005157E-4
26-27	8.438133634616895E-5	2.320486749519646E-4	0.0	5.036511013161959E-4	1.951318403005157E-4
28-29	9.492900338944007E-5	2.3995942523441792E-4	0.0	0.0011312372903908275	1.951318403005157E-4
30-31	9.492900338944007E-5	2.847870101683202E-4	0.0	0.0024101419193874505	1.9776875706133346E-4
32-33	9.492900338944007E-5	2.9006084368995576E-4	0.0	0.0043324542380236124	2.1095334086542237E-4
34-35	9.756592015025785E-5	3.4016226214549355E-4	0.0	0.006842798994322137	2.2677484143032904E-4
36-37	1.028397536718934E-4	3.955375141226669E-4	0.0	0.010529208625945394	2.2677484143032904E-4
38-39	1.0547667043271118E-4	4.1663284820920915E-4	0.0	0.016077281490706002	2.294117581911468E-4
40-41	1.2129817099761786E-4	4.535496828606581E-4	0.0	0.021918052115917384	2.320486749519646E-4
42-43	1.2657200451925341E-4	4.7991885046883595E-4	0.0	0.02717606413698804	2.3995942523441792E-4
44-45	1.3184583804088897E-4	5.537525197717337E-4	0.0	0.03307748384769822	2.5050709227768904E-4
46-47	1.3711967156252453E-4	6.091277717489071E-4	0.0	0.03950101307705034	2.6896550960341353E-4
48-49	1.3711967156252453E-4	6.22312355552996E-4	0.0	0.04589817313879427	2.9006084368995576E-4
50-51	1.397565883233423E-4	6.882352745734405E-4	0.0	0.05193407560430617	2.9006084368995576E-4
52-53	1.423935050841601E-4	6.961460248558938E-4	0.0	0.05763772655795503	3.11156177776498E-4
54-55	1.423935050841601E-4	7.066936918991649E-4	0.0	0.06375273652629146	3.34888428623858E-4
56-57	1.423935050841601E-4	7.119675254208005E-4	0.0	0.07001277691647287	3.375253453846758E-4
58-59	1.423935050841601E-4	7.119675254208005E-4	0.0	0.07618052522002565	3.375253453846758E-4
60-61	1.6085192240988458E-4	7.225151924640716E-4	0.0	0.08192900375860841	3.507099291887647E-4
62-63	1.7403650621397346E-4	7.356997762681605E-4	0.0	0.08735841536913222	3.5862067947121804E-4
64-65	1.819472564964268E-4	7.910750282453338E-4	0.0	0.09294867890206591	3.638945129928536E-4
66-67	1.8985800677888013E-4	7.963488617669694E-4	0.0	0.09885537244629775	3.6916834651448915E-4
68-69	2.0831642410460457E-4	8.174441958535116E-4	0.0	0.10449573739768697	3.6916834651448915E-4
70-71	2.2150100790869348E-4	8.279918628967828E-4	0.0	0.10998316117694876	3.6916834651448915E-4
72-73	2.2677484143032904E-4	8.332656964184184E-4	0.0	0.11460831317542315	4.113590146875736E-4
74-75	2.320486749519646E-4	8.411764467008717E-4	0.0	0.11853204531552002	4.166328482092092E-4
76-77	2.320486749519646E-4	8.991886154388628E-4	0.0	0.12227383019912044	4.19269764970027E-4
78-79	2.3732250847360015E-4	9.229208662862229E-4	0.0	0.12550669014788304	4.2190668173084473E-4
80-81	2.452332587560535E-4	9.229208662862229E-4	0.0	0.12697808970041935	4.2981743201329805E-4
82-83	2.478701755168713E-4	9.229208662862229E-4	0.0	0.12758721747216828	4.4300201581738696E-4
84-85	2.478701755168713E-4	9.229208662862229E-4	0.0	0.12826754199645926	4.4563893257820476E-4
86-87	2.478701755168713E-4	9.33468533329494E-4	0.0	0.12855760284014922	4.535496828606581E-4
88-89	2.478701755168713E-4	0.001004665285871574	0.0	0.128676264094386	4.588235163822936E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19025	0.0	47.70291	1
GTATCAA	33470	0.0	35.39282	1
TCAACGC	39815	0.0	28.814291	4
ATCAACG	40180	0.0	28.531643	3
CAACGCA	41125	0.0	27.886375	5
AACGCAG	42390	0.0	27.123726	6
TATCAAC	43580	0.0	26.623682	2
ACGCAGA	49665	0.0	23.1337	7
CGCAGAG	50580	0.0	22.715208	8
GTACATG	47710	0.0	21.855265	1
TACATGG	47785	0.0	21.390684	2
ACATGGG	47670	0.0	20.834572	3
CATGGGG	35110	0.0	18.675198	4
GCAGAGT	61935	0.0	18.469183	9
GAGTACT	37850	0.0	17.650427	12-13
AGAGTAC	55405	0.0	17.448668	10-11
CAGAGTA	59820	0.0	16.883656	10-11
GTGGTAT	10510	0.0	15.976113	1
AGTACTT	41505	0.0	15.696605	12-13
GTACTTT	43525	0.0	15.407595	14-15
>>END_MODULE