FastQCFastQC Report
Fri 27 May 2016
SRR2139945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529708
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65450.047.202531
GTATCAA116550.035.235951
TCAACGC138100.029.045274
ATCAACG140700.028.4790333
CAACGCA142600.028.1563055
AACGCAG147450.027.2864956
TATCAAC148850.027.2550962
GTACATG181500.023.381711
ACGCAGA173550.023.0163157
TACATGG180500.022.9131152
CGCAGAG176600.022.6188098
ACATGGG182900.022.0670663
CATGGGG140650.020.695234
GCAGAGT215800.018.5101099
ACCGAAC8100.017.9418968
GAGTACT125450.017.89013912-13
AGAGTAC190450.017.15849310-11
CAGAGTA209550.016.79335210-11
ATGGGGG90950.016.161045
GTACTTT144100.015.71874314-15
Produced by FastQC (version 0.11.5)