FastQCFastQC Report
Fri 27 May 2016
SRR2139944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8145832
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89450.047.0019721
GTATCAA152600.033.8487241
TCAACGC178900.028.1771624
ATCAACG181450.027.7581793
CAACGCA184950.027.3443585
AACGCAG190400.026.5616536
TATCAAC193500.026.3302212
ACGCAGA221150.022.8872347
GTACATG214150.022.727421
CGCAGAG224400.022.5372988
TACATGG212850.022.262952
ACATGGG215450.021.3782753
CATGGGG162150.019.7715644
GCAGAGT275450.018.375439
GAGTACT166500.017.82423812-13
CAGAGTA265400.017.2374310-11
AGAGTAC243000.017.13892210-11
GTGGTAT46450.016.6757851
ACCGAAC9500.016.567968
TATACTG45100.015.9800835
Produced by FastQC (version 0.11.5)