##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8145832
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50409988813911	34.0	31.0	34.0	31.0	34.0
2	32.70344625324952	34.0	31.0	34.0	31.0	34.0
3	32.80617277645795	34.0	31.0	34.0	31.0	34.0
4	36.25851637991061	37.0	37.0	37.0	35.0	37.0
5	36.109526319717865	37.0	35.0	37.0	35.0	37.0
6	36.15433500224409	37.0	36.0	37.0	35.0	37.0
7	36.14500925135701	37.0	36.0	37.0	35.0	37.0
8	36.11425438187284	37.0	36.0	37.0	35.0	37.0
9	37.984296508938556	39.0	38.0	39.0	35.0	39.0
10-11	37.873229462625794	39.0	38.0	39.0	35.0	39.0
12-13	37.893092627002375	39.0	38.0	39.0	35.0	39.0
14-15	39.26501540910738	40.0	39.0	41.0	36.0	41.0
16-17	39.24312617790301	40.0	39.0	41.0	36.0	41.0
18-19	39.28039069551152	40.0	39.0	41.0	36.0	41.0
20-21	39.28103533111212	40.0	39.0	41.0	36.0	41.0
22-23	39.246545333752564	40.0	39.0	41.0	36.0	41.0
24-25	39.252643309223586	40.0	39.0	41.0	36.0	41.0
26-27	39.13126441140436	40.0	39.0	41.0	36.0	41.0
28-29	39.0478730200156	40.0	39.0	41.0	36.0	41.0
30-31	38.95194385458252	40.0	38.5	41.0	35.0	41.0
32-33	38.86674960870333	40.0	38.0	41.0	35.0	41.0
34-35	38.806593121909415	40.0	38.0	41.0	35.0	41.0
36-37	38.77667526408432	40.0	38.0	41.0	35.0	41.0
38-39	38.71343496184373	40.0	38.0	41.0	35.0	41.0
40-41	38.69035924210372	40.0	38.0	41.0	35.0	41.0
42-43	38.6665775274756	40.0	38.0	41.0	35.0	41.0
44-45	38.605194172146035	40.0	38.0	41.0	35.0	41.0
46-47	38.53160250295497	40.0	38.0	41.0	34.0	41.0
48-49	38.499420891971084	40.0	38.0	41.0	34.0	41.0
50-51	38.43917929334834	40.0	38.0	41.0	34.0	41.0
52-53	38.27717558239303	40.0	38.0	41.0	34.0	41.0
54-55	38.141815512190036	40.0	37.0	41.0	34.0	41.0
56-57	38.003628878707616	40.0	37.0	41.0	33.0	41.0
58-59	37.85433280190585	39.5	36.5	41.0	33.0	41.0
60-61	37.73103908568308	39.0	36.0	41.0	33.0	41.0
62-63	37.533822504587235	39.0	36.0	41.0	33.0	41.0
64-65	37.31165764136271	39.0	35.0	40.0	33.0	41.0
66-67	37.08329854402576	38.5	35.0	40.0	32.5	41.0
68-69	36.81249404251086	38.0	35.0	40.0	32.0	41.0
70-71	36.51467427242925	37.0	35.0	40.0	32.0	41.0
72-73	36.20332459546425	37.0	35.0	39.0	32.0	41.0
74-75	35.806295133986545	36.0	34.5	39.0	31.5	40.5
76-77	33.83256543488846	34.5	32.0	36.5	29.0	39.0
78-79	34.949331869725384	35.0	34.0	37.0	31.0	39.0
80-81	34.91555690823854	35.0	34.0	37.0	31.0	39.0
82-83	34.64503107037403	35.0	34.0	36.5	31.0	39.0
84-85	34.330727775056125	35.0	34.0	36.0	31.0	37.5
86-87	34.151380432193314	35.0	34.0	36.0	31.0	37.0
88-89	33.96304465442232	35.0	34.0	35.5	31.0	37.0
90-91	33.79596745528575	35.0	34.0	35.0	30.0	36.0
92-93	33.71885292790071	35.0	34.0	35.0	30.0	36.0
94-95	33.6518994285954	35.0	34.0	35.0	30.0	36.0
96-97	33.66591327067964	35.0	34.0	35.0	31.0	36.0
98-99	33.697094970062366	35.0	34.0	35.0	31.0	35.0
100-101	32.45996147653658	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	4.0
19	13.0
20	30.0
21	144.0
22	530.0
23	1656.0
24	4360.0
25	9868.0
26	18478.0
27	30973.0
28	47145.0
29	66335.0
30	91058.0
31	121549.0
32	163861.0
33	225867.0
34	337099.0
35	526733.0
36	970063.0
37	1922785.0
38	2484412.0
39	1103190.0
40	19676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36819517024166	20.088580270253548	13.245522372668624	24.297702186836165
2	14.805745237131534	21.53344310946099	40.845742688537925	22.815068964869553
3	18.142836729262278	26.40459562632767	30.680647477139228	24.77192016727082
4	12.27929252807079	17.85980030610515	39.447002277435075	30.413904888388988
5	12.759116438678205	38.156686010360914	36.18944077609499	12.89475677486589
6	29.694283206071542	38.390106713797664	18.992887997469126	12.92272208266167
7	26.07164007310733	32.777990019926754	23.85928165471618	17.29108825224974
8	24.415308344193694	36.68386482804949	21.703823501393103	17.197003326363717
9	25.675646146397323	16.26511570579899	21.399348771248906	36.65988937655478
10-11	23.987862215671065	26.706237555708135	29.616975760400788	19.688924468220016
12-13	24.766391510114367	24.406481534565046	29.546187495450106	21.28093945987048
14-15	21.827229392216633	25.798801349893136	27.055290866615188	25.318678391275046
16-17	21.50542142288228	28.876399611482288	27.73608024324587	21.88209872238956
18-19	21.52780898014302	28.228708222078996	29.260608292667957	20.982874505110026
20-21	22.283709293367174	27.446059489477932	29.14169001640103	21.12854120075386
22-23	21.97896453142275	27.401414273989033	28.98539131950818	21.634229875080035
24-25	21.70522048665234	27.494166775300542	29.117448561424457	21.683164176622657
26-27	21.67345292430758	27.677094786333324	29.003311487321366	21.646140802037735
28-29	21.655355243206877	27.785428293059105	28.949754065989303	21.609462397744718
30-31	21.90178208470847	27.61921225225807	28.878847221017068	21.600158442016397
32-33	21.652849208337717	27.831219944186564	28.808109553270107	21.707821294205612
34-35	21.844576747384192	27.811504650550447	28.852494211384645	21.491424390680717
36-37	21.795231580984993	27.807815920606505	28.777272554924277	21.619679943484222
38-39	21.644481261174498	27.90050277305517	28.837755302357394	21.61726066341294
40-41	21.881325943445823	27.758781593745752	28.727262297454796	21.632630165353632
42-43	21.759819255034213	27.881854260216205	28.53523829324518	21.8230881915044
44-45	21.930856692878102	27.98533651478616	28.33989898116712	21.743907811168615
46-47	21.926446379235134	27.963711079989693	28.143078490552032	21.966764050223137
48-49	21.842748600881052	28.102734840992046	28.161837586319542	21.89267897180736
50-51	21.901419537446095	28.19425800476592	27.997297142160853	21.90702531562713
52-53	22.067971274995234	28.05723939695593	27.90686581407021	21.96792351397863
54-55	21.976368232481537	28.017654519742376	27.988821072065168	22.01715617571092
56-57	21.95800425681759	28.14920024215637	27.944255464563817	21.948540036462227
58-59	21.949318338414813	28.17108725028104	27.921054891871645	21.9585395194325
60-61	22.019561061410954	28.115946149682387	27.96933237277829	21.895160416128366
62-63	21.992383983467757	28.17526273185903	27.909028644345607	21.923324640327607
64-65	21.962140892167646	28.152914883313663	27.865371830601294	22.019572393917397
66-67	22.011114580945573	28.177467772081798	27.760275199680954	22.05114244729167
68-69	21.979591961583818	28.151861983205826	27.838998610407963	22.0295474448024
70-71	21.990246112131988	27.923514738068473	27.879062737119863	22.207176412679676
72-73	22.04136958712211	27.968862826529644	27.885318539019654	22.10444904732859
74-75	21.90377991893875	28.070636853668667	27.839305842539012	22.186277384853568
76-77	22.01694972652873	27.969028825566824	27.80607516454674	22.207946283357707
78-79	21.996751611513314	28.023669264777585	27.76489069675202	22.214688426957085
80-81	22.013845366569836	28.009302383422835	27.763927350707913	22.212924899299413
82-83	22.040940624283827	27.968380369953373	27.846240944588573	22.144438061174228
84-85	22.193421745694682	27.8876548384247	27.78942472406562	22.12949869181499
86-87	21.995319671866852	27.899525745719455	27.921246159043772	22.183908423369918
88-89	22.075453480493227	27.86946836680375	27.865469518608744	22.18960863409428
90-91	22.121937391581834	27.888605479011353	27.886956120911144	22.102501008495672
92-93	22.156023072156103	27.77900483229961	27.917458955467634	22.14751314007666
94-95	22.004425223504505	27.745547762206353	28.04523346229232	22.204793551996826
96-97	22.042696006005226	27.794470152105255	28.01833806353478	22.144495778354738
98-99	22.279612285663468	28.39451035257552	28.05269725827579	21.273180103485224
100-101	26.25922083199162	35.34578705555207	28.82596414681138	9.569027965644928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1161.0
1	1125.0
2	1843.0
3	2868.0
4	3951.5
5	6517.5
6	8906.0
7	10654.0
8	11853.0
9	11792.5
10	10838.0
11	9709.0
12	10259.0
13	13237.0
14	19891.0
15	29098.0
16	36860.0
17	41636.0
18	43330.0
19	42337.0
20	40442.5
21	39116.5
22	39530.5
23	43847.5
24	52690.0
25	65193.5
26	81193.5
27	98724.0
28	118087.0
29	139781.5
30	160267.0
31	179134.5
32	197811.5
33	216096.5
34	233128.5
35	248978.0
36	266246.0
37	281052.5
38	292570.5
39	303411.0
40	313729.5
41	323603.0
42	330010.0
43	335556.5
44	341523.5
45	346427.0
46	348998.0
47	348761.0
48	345707.5
49	338473.5
50	329661.5
51	318878.5
52	303831.0
53	287681.0
54	271610.5
55	252946.0
56	232403.5
57	210373.0
58	187710.0
59	163921.0
60	138326.5
61	114057.5
62	91650.0
63	71866.5
64	55164.0
65	42196.5
66	32905.0
67	25980.5
68	20103.5
69	14499.0
70	10317.5
71	7576.0
72	5355.0
73	3712.5
74	2620.5
75	1841.5
76	1259.0
77	826.5
78	537.5
79	367.0
80	248.5
81	167.5
82	123.5
83	87.5
84	57.0
85	36.5
86	24.0
87	19.0
88	14.5
89	10.5
90	7.0
91	3.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0020010233454360463
3	0.0
4	0.003253197463438971
5	3.069054210791482E-4
6	3.069054210791482E-4
7	0.0
8	0.0
9	0.0
10-11	3.5601028845181194E-4
12-13	5.524297579424668E-4
14-15	2.4552433686331857E-5
16-17	0.0
18-19	0.0010066497811396062
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.071537773372237E-5
28-29	0.0
30-31	4.926677187175243E-5
32-33	9.88363502305605E-4
34-35	7.131458768416915E-4
36-37	3.492311675845626E-4
38-39	0.001716584806111746
40-41	6.345574666029232E-6
42-43	1.2810068508886888E-5
44-45	1.2950617546960073E-4
46-47	1.311356828782499E-5
48-49	5.9733227424108766E-5
50-51	1.879778371444609E-4
52-53	6.7851986590819E-6
54-55	0.0032577168109983808
56-57	0.0029626850169039566
58-59	0.0013343495927108555
60-61	9.240654215572237E-5
62-63	0.0
64-65	1.0911609705396722E-4
66-67	3.681737638786782E-5
68-69	1.9397252752171746E-4
70-71	0.0
72-73	0.0
74-75	1.4736413647097952E-4
76-77	1.571470518466625E-5
78-79	4.538836675456615E-4
80-81	0.0
82-83	1.7200399147548217E-4
84-85	2.490831456976159E-5
86-87	0.0016416321965998513
88-89	0.0
90-91	9.548906198837394E-5
92-93	2.6483184105004767E-5
94-95	2.1557490999073834E-4
96-97	3.663508234101107E-5
98-99	9.536928704591343E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	212.0
20-21	577.0
22-23	1215.0
24-25	3505.0
26-27	5036.0
28-29	10849.0
30-31	23560.0
32-33	29312.0
34-35	39671.0
36-37	61763.0
38-39	72715.0
40-41	72106.0
42-43	80633.0
44-45	94841.0
46-47	94073.0
48-49	87763.0
50-51	80014.0
52-53	77302.0
54-55	82793.0
56-57	86532.0
58-59	86017.0
60-61	82740.0
62-63	79351.0
64-65	81242.0
66-67	87989.0
68-69	88311.0
70-71	86841.0
72-73	81821.0
74-75	83110.0
76-77	83295.0
78-79	86832.0
80-81	88309.0
82-83	83122.0
84-85	82201.0
86-87	86151.0
88-89	90742.0
90-91	95809.0
92-93	95868.0
94-95	102420.0
96-97	151759.0
98-99	645919.0
100-101	4691511.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.94986557795859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.82761468123552	78.97448887300045
2	6.567816090832	11.421414524771917
3	1.4974653768120942	3.9061323966437604
4	0.512374763117202	1.7820366711431632
5	0.25830456485202635	1.1229773596028387
6	0.11689100225830688	0.6098194160179581
7	0.06401159459971262	0.38960596821130544
8	0.039457372769290844	0.27446506066793935
9	0.02486969345814039	0.19461748528353082
>10	0.08954587976977535	1.1716818137700968
>50	0.0013162754820882474	0.0762493340939887
>100	2.906742085715053E-4	0.05085613380082873
>500	4.203060511568404E-5	0.025654962992243877
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2276216843165928E-5	0.0
4	0.0	4.910486737266371E-5	0.0	1.2276216843165928E-5	0.0
5	0.0	4.910486737266371E-5	0.0	1.2276216843165928E-5	0.0
6	0.0	4.910486737266371E-5	0.0	1.2276216843165928E-5	0.0
7	0.0	4.910486737266371E-5	0.0	2.4552433686331857E-5	0.0
8	0.0	6.138108421582964E-5	0.0	2.4552433686331857E-5	0.0
9	0.0	1.9641946949065485E-4	0.0	6.138108421582964E-5	0.0
10-11	0.0	1.9641946949065485E-4	0.0	7.365730105899557E-5	0.0
12-13	0.0	1.9641946949065485E-4	0.0	8.59335179021615E-5	0.0
14-15	0.0	2.8235298739281636E-4	0.0	8.59335179021615E-5	0.0
16-17	0.0	2.8235298739281636E-4	0.0	9.820973474532743E-5	0.0
18-19	0.0	2.884910958143993E-4	0.0	1.1048595158849336E-4	6.138108421582964E-6
20-21	0.0	2.9462920423598227E-4	0.0	1.2890027685324225E-4	1.2276216843165928E-5
22-23	0.0	3.928389389813097E-4	0.0	1.9641946949065485E-4	1.841432526474889E-5
24-25	0.0	4.2966758951080747E-4	0.0	2.5166244528490155E-4	2.4552433686331857E-5
26-27	0.0	4.5422002319713933E-4	0.0	3.43734071608646E-4	3.069054210791482E-5
28-29	0.0	4.5422002319713933E-4	0.0	0.0010618927569338529	3.682865052949778E-5
30-31	0.0	5.647059747856327E-4	0.0	0.0025166244528490155	3.682865052949778E-5
32-33	0.0	5.647059747856327E-4	0.0	0.004978005929903784	3.682865052949778E-5
34-35	0.0	6.322251674230453E-4	0.0	0.007967264731214688	4.910486737266371E-5
36-37	0.0	7.058824684820408E-4	0.0	0.012761127408470983	4.910486737266371E-5
38-39	0.0	7.611254442762876E-4	0.0	0.02039693428492019	4.910486737266371E-5
40-41	0.0	8.593351790216149E-4	0.0	0.02843171820877229	6.138108421582964E-5
42-43	0.0	8.900257211295298E-4	0.0	0.03563171938728911	6.138108421582964E-5
44-45	0.0	9.820973474532744E-4	0.0	0.043513050600601635	6.138108421582964E-5
46-47	0.0	0.0010557546485122698	0.0	0.05221688834240627	6.138108421582964E-5
48-49	0.0	0.0010557546485122698	0.0	0.060834792566308755	6.138108421582964E-5
50-51	0.0	0.001184654925365512	0.0	0.06956318274179973	6.138108421582964E-5
52-53	0.0	0.0012030692506302609	0.0	0.07794783884568207	6.75191926374126E-5
54-55	0.0	0.001209207359051844	0.0	0.08631408062429964	7.365730105899557E-5
56-57	0.0	0.001227621684316593	0.0	0.0946925986197604	7.365730105899557E-5
58-59	0.0	0.001233759792738176	0.0	0.10289924957941682	7.365730105899557E-5
60-61	0.0	0.0012460360095813416	0.0	0.11117955784013223	7.365730105899557E-5
62-63	0.0	0.0012890027685324224	0.0	0.11887674580079727	7.365730105899557E-5
64-65	0.0	0.0013872125032777499	0.0	0.1262854426656479	7.365730105899557E-5
66-67	0.0	0.0013872125032777499	0.0	0.1337493825062928	7.365730105899557E-5
68-69	0.0	0.0014179030453856647	0.0	0.14156319452696792	7.365730105899557E-5
70-71	0.0	0.0014363173706504137	0.0	0.14881230057285738	7.365730105899557E-5
72-73	0.0	0.0014670079127583283	0.0	0.1542874932849094	7.365730105899557E-5
74-75	0.0	0.0015161127801309922	0.0	0.15887879838425345	7.365730105899557E-5
76-77	0.0	0.0016143225148763196	0.0	0.16306498832777302	7.365730105899557E-5
78-79	0.0	0.0016511511654058175	0.0	0.16669874851335015	7.365730105899557E-5
80-81	0.0	0.0016572892738274004	0.0	0.16855859536508977	7.365730105899557E-5
82-83	0.0	0.0016572892738274004	0.0	0.16925220161672866	7.365730105899557E-5
84-85	0.0	0.0016572892738274004	0.0	0.1697800789409848	7.365730105899557E-5
86-87	0.0	0.0016818417075137322	0.0	0.17001332706100494	7.365730105899557E-5
88-89	0.0	0.0017677752254158937	0.0	0.17012381301259344	7.365730105899557E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8945	0.0	47.001972	1
GTATCAA	15260	0.0	33.848724	1
TCAACGC	17890	0.0	28.177162	4
ATCAACG	18145	0.0	27.758179	3
CAACGCA	18495	0.0	27.344358	5
AACGCAG	19040	0.0	26.561653	6
TATCAAC	19350	0.0	26.330221	2
ACGCAGA	22115	0.0	22.887234	7
GTACATG	21415	0.0	22.72742	1
CGCAGAG	22440	0.0	22.537298	8
TACATGG	21285	0.0	22.26295	2
ACATGGG	21545	0.0	21.378275	3
CATGGGG	16215	0.0	19.771564	4
GCAGAGT	27545	0.0	18.37543	9
GAGTACT	16650	0.0	17.824238	12-13
CAGAGTA	26540	0.0	17.23743	10-11
AGAGTAC	24300	0.0	17.138922	10-11
GTGGTAT	4645	0.0	16.675785	1
ACCGAAC	950	0.0	16.56796	8
TATACTG	4510	0.0	15.980083	5
>>END_MODULE