FastQCFastQC Report
Fri 27 May 2016
SRR2139943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7363469
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76000.046.757541
GTATCAA139700.036.0680731
TCAACGC164350.029.726884
ATCAACG166450.029.4015393
CAACGCA169350.028.8736295
AACGCAG173600.028.2144186
TATCAAC176350.028.0795882
ACGCAGA203200.024.0435317
CGCAGAG206350.023.6965488
GTACATG201800.022.5498451
TACATGG199350.022.1836782
ACATGGG200450.021.525213
CATGGGG149300.020.3378684
GCAGAGT246850.019.7249269
GAGTACT151350.018.28600112-13
AGAGTAC223050.018.01825110-11
CTTATAC55500.017.292871
CAGAGTA244100.017.21860710-11
ATGGGGG97200.016.3430865
AGTACTT167600.016.17982112-13
Produced by FastQC (version 0.11.5)