##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7363469
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48153825323363	34.0	31.0	34.0	31.0	34.0
2	32.69129672441073	34.0	31.0	34.0	31.0	34.0
3	32.79785288700204	34.0	31.0	34.0	31.0	34.0
4	36.25505369819578	37.0	37.0	37.0	35.0	37.0
5	36.09662796163058	37.0	35.0	37.0	35.0	37.0
6	36.14098029067549	37.0	36.0	37.0	35.0	37.0
7	36.13371021185802	37.0	35.0	37.0	35.0	37.0
8	36.10376114844783	37.0	35.0	37.0	35.0	37.0
9	37.981928354692606	39.0	38.0	39.0	35.0	39.0
10-11	37.86920546552176	39.0	38.0	39.0	35.0	39.0
12-13	37.892212013115014	39.0	38.0	39.0	35.0	39.0
14-15	39.2627603239723	40.0	39.0	41.0	36.0	41.0
16-17	39.238360954598974	40.0	39.0	41.0	36.0	41.0
18-19	39.27700279582898	40.0	39.0	41.0	36.0	41.0
20-21	39.281375521484975	40.0	39.0	41.0	36.0	41.0
22-23	39.24825875417957	40.0	39.0	41.0	36.0	41.0
24-25	39.25452079290984	40.0	39.0	41.0	36.0	41.0
26-27	39.13430317930528	40.0	39.0	41.0	36.0	41.0
28-29	39.050392191244754	40.0	39.0	41.0	36.0	41.0
30-31	38.954972717212534	40.0	38.5	41.0	35.0	41.0
32-33	38.8668144542485	40.0	38.0	41.0	35.0	41.0
34-35	38.80476904523556	40.0	38.0	41.0	35.0	41.0
36-37	38.77173954826068	40.0	38.0	41.0	35.0	41.0
38-39	38.71046429147391	40.0	38.0	41.0	35.0	41.0
40-41	38.69175779343614	40.0	38.0	41.0	35.0	41.0
42-43	38.66923811900732	40.0	38.0	41.0	35.0	41.0
44-45	38.607346184768424	40.0	38.0	41.0	35.0	41.0
46-47	38.53407473204311	40.0	38.0	41.0	34.0	41.0
48-49	38.50223543821469	40.0	38.0	41.0	34.0	41.0
50-51	38.43829364093311	40.0	38.0	41.0	34.0	41.0
52-53	38.276000378001704	40.0	38.0	41.0	34.0	41.0
54-55	38.14112790724245	40.0	37.0	41.0	34.0	41.0
56-57	38.00083400896233	40.0	37.0	41.0	33.0	41.0
58-59	37.84395560345096	39.5	36.5	41.0	33.0	41.0
60-61	37.71755890529268	39.0	36.0	41.0	33.0	41.0
62-63	37.52099287223085	39.0	36.0	41.0	33.0	41.0
64-65	37.30311224693858	39.0	35.0	40.0	33.0	41.0
66-67	37.07759835058492	38.5	35.0	40.0	32.5	41.0
68-69	36.80588080056731	37.5	35.0	40.0	32.0	41.0
70-71	36.5105975945753	37.0	35.0	40.0	32.0	41.0
72-73	36.19960496410464	37.0	35.0	39.0	32.0	41.0
74-75	35.805231486047404	36.0	34.5	39.0	31.5	40.5
76-77	33.83406375083386	34.5	32.0	36.5	29.0	39.0
78-79	34.92931381865455	35.0	34.0	37.0	30.5	39.0
80-81	34.89849182439379	35.0	34.0	37.0	31.0	39.0
82-83	34.63051040744382	35.0	34.0	36.5	31.0	39.0
84-85	34.32082606292371	35.0	34.0	36.0	31.0	37.5
86-87	34.144676419624794	35.0	34.0	36.0	31.0	37.0
88-89	33.95326738273677	35.0	34.0	35.5	31.0	37.0
90-91	33.78824500894052	35.0	34.0	35.0	30.0	36.0
92-93	33.71505890348546	35.0	34.0	35.0	30.0	36.0
94-95	33.64867267189081	35.0	34.0	35.0	30.0	36.0
96-97	33.665907200600905	35.0	34.0	35.0	31.0	36.0
98-99	33.69981818883775	35.0	34.0	35.0	31.0	35.0
100-101	32.46632233359115	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	10.0
20	28.0
21	104.0
22	422.0
23	1493.0
24	3757.0
25	8636.0
26	16282.0
27	27214.0
28	42394.0
29	60395.0
30	83061.0
31	110319.0
32	148482.0
33	205585.0
34	304298.0
35	477028.0
36	879512.0
37	1746835.0
38	2250338.0
39	982041.0
40	15232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38405838335165	20.076950144015	13.155470607671466	24.38352086496188
2	14.821175191110067	21.46525143088095	40.876544960300386	22.83702841770859
3	18.206079227059963	26.3626831320944	30.575344311220704	24.855893329624937
4	12.273446634292249	17.811131840263926	39.2170763311328	30.69834519431102
5	12.729807608995902	38.205692392226354	36.1541382413279	12.910361757449845
6	29.804010135477405	38.295297226487286	18.893256713368558	13.007435924666751
7	26.191160715146623	32.64795438128415	23.682506166590773	17.478378736978453
8	24.45654351230378	36.78282613806074	21.625622379886437	17.13500796974904
9	25.61412290864537	16.24210002106344	21.333395984963065	36.810381085328125
10-11	24.065003243045922	26.69414518850408	29.509925241544448	19.73092632690555
12-13	24.80874397087878	24.322428358096257	29.497871100253846	21.370956570771114
14-15	21.844652546870577	25.740934842115156	26.908204123383168	25.5062084876311
16-17	21.62033954376667	28.856534875070434	27.597142053561985	21.925983527600916
18-19	21.599185374827194	28.100310909932542	29.190149124998392	21.11035459024187
20-21	22.30246418836988	27.38884934170404	29.019699341951892	21.288987127974185
22-23	21.988550383223764	27.40139715504208	28.887685057645022	21.722367404089137
24-25	21.780332825511824	27.52476818586539	28.99772906785001	21.69716992077278
26-27	21.783118490392074	27.67276343483046	28.8919494445198	21.652168630257666
28-29	21.673587264341172	27.816266598587337	28.851846799199205	21.65829933787229
30-31	22.04276912497472	27.595076203414298	28.74077922438902	21.621375447221965
32-33	21.629810061656364	27.85344852799082	28.752340779821694	21.76440063053112
34-35	21.860950678099304	27.839053767193956	28.785805190785403	21.51419036392134
36-37	21.787054583748315	27.80409784469353	28.71606229169892	21.692785279859237
38-39	21.656736859995675	27.832402014656537	28.82352053139046	21.687340593957323
40-41	21.91280810170336	27.69833690075992	28.687122658369486	21.70173233916724
42-43	21.838371375694017	27.78493501965338	28.537932456723762	21.83876114792884
44-45	21.89235626554918	27.92991528867243	28.358730206944138	21.81899823883425
46-47	21.998387537251478	27.86577897799746	28.101250764795505	22.03458271995555
48-49	21.866574231784334	27.992981919249488	28.097646655082446	22.042797193883732
50-51	21.832616279255447	28.0668758300303	28.081507313104897	22.01900057760936
52-53	22.04268801880798	27.967637003589175	28.008576787052696	21.981098190550146
54-55	21.965556307564434	27.938946320500484	27.99530778831425	22.100189583620836
56-57	22.007413774578275	28.10566547452762	27.86302168874426	22.02389906214984
58-59	21.908096297767198	28.166041895265252	27.956849909119924	21.969011897847622
60-61	22.046599108881594	28.18673623282156	27.876765589105467	21.889899069191383
62-63	21.95615226220813	28.263302976088493	27.86990766718405	21.910637094519327
64-65	21.940903183956305	28.22366281415873	27.793426753488877	22.04200724839609
66-67	22.010020754795036	28.212968658603167	27.685780382985815	22.091230203615986
68-69	21.970207071692236	28.145387690834305	27.78680785057081	22.097597386902645
70-71	21.986080720891756	27.931086378835534	27.86926965599166	22.21356324428105
72-73	22.059946985266627	27.97214740228146	27.81699764941079	22.15090796304112
74-75	21.90533586831721	28.09380895709009	27.791635808042443	22.20921936655026
76-77	22.06698404799271	27.993724228583506	27.752632360671765	22.18665936275202
78-79	22.02540283550822	28.012929266840214	27.721471208229104	22.240196689422465
80-81	22.04302638201619	28.006129682024532	27.74652519714529	22.20431873881399
82-83	22.0860946077244	27.963293081096875	27.843089760582203	22.107522550596524
84-85	22.20049826953728	27.903930881906554	27.74205259352393	22.153518255032235
86-87	22.006418033217113	27.94658084179514	27.864884747722407	22.182116377265345
88-89	22.090793005227454	27.909628006413378	27.823968694142042	22.175610294217126
90-91	22.16233468431879	27.879711422432766	27.855185327705918	22.102768565542526
92-93	22.154401493523977	27.809144756225013	27.886781814459642	22.149671935791368
94-95	22.0425583180405	27.77771482384263	27.993593807559364	22.186133050557512
96-97	22.090322680010097	27.794292240843593	27.996113536688704	22.119271542457604
98-99	22.341112996099184	28.361260981432483	28.0472970870694	21.250328935398933
100-101	26.282102969218197	35.336461454644514	28.835957504767094	9.5454780713702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1067.0
1	1027.0
2	1731.5
3	2702.0
4	3687.5
5	5995.5
6	8058.5
7	9604.5
8	10795.5
9	10811.5
10	9779.5
11	8514.0
12	8902.0
13	11592.0
14	17885.5
15	26481.5
16	33363.0
17	37293.0
18	38597.5
19	37937.0
20	36257.5
21	34609.0
22	34896.0
23	38691.5
24	46132.5
25	57130.0
26	71400.0
27	87118.0
28	104181.5
29	122696.0
30	140475.5
31	157551.0
32	175872.5
33	193814.5
34	209067.0
35	223171.5
36	240042.0
37	254453.0
38	265184.5
39	274919.0
40	283633.0
41	291375.0
42	295764.0
43	300258.5
44	305192.0
45	310207.0
46	314469.5
47	316945.5
48	314353.0
49	307716.5
50	301676.5
51	292396.5
52	278790.0
53	264736.5
54	250692.0
55	233863.5
56	215357.5
57	195090.5
58	173511.5
59	151430.0
60	128168.5
61	105471.0
62	84461.0
63	65862.0
64	50017.5
65	37960.5
66	29399.5
67	23047.5
68	17809.5
69	12962.5
70	9085.5
71	6409.0
72	4426.5
73	2959.0
74	1918.0
75	1296.5
76	882.5
77	602.5
78	413.5
79	291.5
80	218.0
81	149.5
82	107.5
83	75.0
84	47.5
85	31.5
86	22.0
87	18.5
88	15.0
89	10.5
90	7.0
91	6.0
92	5.5
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0023494361149615757
3	0.0
4	0.0033679777832975196
5	3.666750006009396E-4
6	3.666750006009396E-4
7	0.0
8	0.0
9	0.0
10-11	2.8519166713406415E-4
12-13	5.568027786903157E-4
14-15	1.3580555577812578E-5
16-17	0.0
18-19	0.0011203958351695376
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.154629165520489E-5
28-29	0.0
30-31	0.0
32-33	0.001065998422322335
34-35	8.437753968676999E-4
36-37	4.1394429303282085E-4
38-39	0.001808780837887113
40-41	7.020880379082607E-6
42-43	7.086767403647956E-6
44-45	6.44747865974112E-5
46-47	2.9014166094060008E-5
48-49	1.4684632825440831E-5
50-51	1.336884991126426E-4
52-53	1.5012752958319268E-5
54-55	0.0033152705458012596
56-57	0.002908632337284085
58-59	0.0015537470630296142
60-61	2.0450721312672025E-4
62-63	3.184548063269327E-5
64-65	1.369535252240197E-4
66-67	6.527430588730995E-5
68-69	2.5633452217425917E-4
70-71	0.0
72-73	0.0
74-75	1.375153232895006E-4
76-77	8.706191207634911E-6
78-79	4.145901141604753E-4
80-81	0.0
82-83	7.257553707258225E-5
84-85	7.35642668929994E-5
86-87	0.001771309246448688
88-89	0.0
90-91	1.441578724758317E-4
92-93	9.771812542238661E-6
94-95	1.988015052454771E-4
96-97	0.0
98-99	2.1094049189424244E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	171.0
20-21	482.0
22-23	1077.0
24-25	3044.0
26-27	4547.0
28-29	9977.0
30-31	21040.0
32-33	26411.0
34-35	36352.0
36-37	56373.0
38-39	66287.0
40-41	65045.0
42-43	72719.0
44-45	84939.0
46-47	85143.0
48-49	79346.0
50-51	72517.0
52-53	69169.0
54-55	74154.0
56-57	78875.0
58-59	79085.0
60-61	77964.0
62-63	73649.0
64-65	76141.0
66-67	82125.0
68-69	80271.0
70-71	77730.0
72-73	73111.0
74-75	74564.0
76-77	73428.0
78-79	78008.0
80-81	79362.0
82-83	74621.0
84-85	73926.0
86-87	76799.0
88-89	81344.0
90-91	85951.0
92-93	85159.0
94-95	91206.0
96-97	136015.0
98-99	581943.0
100-101	4243399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.7617561999416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.61085931715877	78.61557285142537
2	6.728174195421486	11.674964184277945
3	1.534889800811878	3.995092040754513
4	0.5504719136704387	1.910396398751596
5	0.23232933300199413	1.007865047400703
6	0.12168020365808177	0.633431289848504
7	0.06640977825071694	0.40332802929166217
8	0.04163886282797526	0.28901286920988806
9	0.026761862011032485	0.20897155325319117
>10	0.0851960156767643	1.1114208984588547
>50	0.0012210972682978954	0.07161370060094825
>100	3.4430171601631756E-4	0.06439158969172543
>500	2.3318526585981457E-5	0.013939547035118032
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.3580555577812577E-5	0.0	1.3580555577812577E-5	0.0
5	0.0	2.7161111155625153E-5	0.0	1.3580555577812577E-5	0.0
6	8.148333346687546E-5	2.7161111155625153E-5	0.0	1.3580555577812577E-5	0.0
7	8.148333346687546E-5	2.7161111155625153E-5	0.0	1.3580555577812577E-5	0.0
8	8.148333346687546E-5	4.074166673343773E-5	0.0	6.790277788906288E-5	0.0
9	8.148333346687546E-5	8.148333346687546E-5	0.0	1.0864444462250061E-4	0.0
10-11	8.827361125578175E-5	9.506388904468804E-5	0.0	1.5617638914484463E-4	0.0
12-13	9.506388904468804E-5	9.506388904468804E-5	0.0	1.6975694472265722E-4	0.0
14-15	1.2222500020031318E-4	1.2222500020031318E-4	0.0	1.969180558782824E-4	0.0
16-17	2.376597226117201E-4	1.2222500020031318E-4	0.0	2.0370833366718867E-4	0.0
18-19	2.7161111155625154E-4	1.4259583356703205E-4	0.0	2.376597226117201E-4	0.0
20-21	2.7161111155625154E-4	1.629666669337509E-4	0.0	2.851916671340641E-4	0.0
22-23	2.987722227118767E-4	1.969180558782824E-4	0.0	3.5309444502312705E-4	0.0
24-25	3.1235277828968927E-4	2.3765972261172012E-4	0.0	3.870458339676584E-4	0.0
26-27	3.259333338675018E-4	2.58030555978439E-4	0.0	4.685291674345339E-4	0.0
28-29	3.3951388944531444E-4	2.58030555978439E-4	0.0	0.0013037333354700073	0.0
30-31	3.5309444502312705E-4	3.3951388944531444E-4	0.0	0.003245752783097206	0.0
32-33	3.666750006009396E-4	3.3951388944531444E-4	0.0	0.006335329177049567	0.0
34-35	3.666750006009396E-4	4.0062638954547103E-4	0.0	0.01011751390547037	0.0
36-37	3.8025555617875217E-4	4.685291674345339E-4	0.0	0.01699606530563244	0.0
38-39	3.8025555617875217E-4	5.024805563790654E-4	0.0	0.02729691671140328	0.0
40-41	3.8025555617875217E-4	5.839638898459408E-4	0.0	0.03862989034108788	0.0
42-43	3.8025555617875217E-4	6.382861121571911E-4	0.0	0.04845542230163527	0.0
44-45	3.8025555617875217E-4	7.333500012018791E-4	0.0	0.05959147787544159	0.0
46-47	3.870458339676584E-4	8.012527790909421E-4	0.0	0.07227571678511854	0.0
48-49	3.938361117565647E-4	8.352041680354735E-4	0.0	0.08489884319469532	1.3580555577812577E-5
50-51	4.277875007010962E-4	9.098972237134426E-4	0.0	0.09722998765934915	1.3580555577812577E-5
52-53	4.4815833406781506E-4	9.098972237134426E-4	0.0	0.10835925295536655	1.3580555577812577E-5
54-55	4.4815833406781506E-4	9.234777792912552E-4	0.0	0.12005211130786318	1.3580555577812577E-5
56-57	4.4815833406781506E-4	9.438486126579741E-4	0.0	0.12940911410097605	1.3580555577812577E-5
58-59	4.4815833406781506E-4	9.778000016025055E-4	0.0	0.13721114328042938	1.3580555577812577E-5
60-61	4.5494861185672137E-4	9.913805571803182E-4	0.0	0.14510144607113848	1.3580555577812577E-5
62-63	5.024805563790654E-4	9.981708349692245E-4	0.0	0.15248926830546852	2.7161111155625153E-5
64-65	5.024805563790654E-4	0.0010457027794915684	0.0	0.15940856137236403	2.7161111155625153E-5
66-67	5.024805563790654E-4	0.0010796541684361	0.0	0.16687107666237205	2.7161111155625153E-5
68-69	5.024805563790654E-4	0.0011203958351695374	0.0	0.17422494750775755	2.7161111155625153E-5
70-71	5.024805563790654E-4	0.0011407666685362564	0.0	0.18134794890832023	2.7161111155625153E-5
72-73	5.296416675346905E-4	0.0011407666685362564	0.0	0.18708573363994607	2.7161111155625153E-5
74-75	5.296416675346905E-4	0.001154347224114069	0.0	0.1912413836467567	2.7161111155625153E-5
76-77	5.568027786903157E-4	0.0011815083352696943	0.0	0.19492171420834392	2.7161111155625153E-5
78-79	5.703833342681282E-4	0.0011950888908475068	0.0	0.19860204476993115	2.7161111155625153E-5
80-81	5.703833342681282E-4	0.0011950888908475068	0.0	0.2002113406059019	2.7161111155625153E-5
82-83	5.703833342681282E-4	0.001201879168636413	0.0	0.20070024060670316	2.7161111155625153E-5
84-85	5.703833342681282E-4	0.0012086694464253193	0.0	0.20112123782961536	2.7161111155625153E-5
86-87	5.703833342681282E-4	0.001249411113158757	0.0	0.20133173644107144	2.7161111155625153E-5
88-89	5.703833342681282E-4	0.0012833625021032885	0.0	0.20138605866338272	2.7161111155625153E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7600	0.0	46.75754	1
GTATCAA	13970	0.0	36.068073	1
TCAACGC	16435	0.0	29.72688	4
ATCAACG	16645	0.0	29.401539	3
CAACGCA	16935	0.0	28.873629	5
AACGCAG	17360	0.0	28.214418	6
TATCAAC	17635	0.0	28.079588	2
ACGCAGA	20320	0.0	24.043531	7
CGCAGAG	20635	0.0	23.696548	8
GTACATG	20180	0.0	22.549845	1
TACATGG	19935	0.0	22.183678	2
ACATGGG	20045	0.0	21.52521	3
CATGGGG	14930	0.0	20.337868	4
GCAGAGT	24685	0.0	19.724926	9
GAGTACT	15135	0.0	18.286001	12-13
AGAGTAC	22305	0.0	18.018251	10-11
CTTATAC	5550	0.0	17.29287	1
CAGAGTA	24410	0.0	17.218607	10-11
ATGGGGG	9720	0.0	16.343086	5
AGTACTT	16760	0.0	16.179821	12-13
>>END_MODULE