FastQCFastQC Report
Fri 27 May 2016
SRR2139942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10839018
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107800.045.5849881
GTATCAA191150.034.0853271
TCAACGC224550.028.1803024
ATCAACG225250.028.1287783
CAACGCA231300.027.3566595
AACGCAG238950.026.5149366
TATCAAC246650.025.9365252
ACGCAGA283300.022.42147
CGCAGAG285350.022.2460428
GTACATG301700.021.7037031
TACATGG295750.021.5065822
ACATGGG300100.020.8957463
CATGGGG213850.019.1870174
GCAGAGT349500.018.0928829
GAGTACT197200.017.19030812-13
AGAGTAC310100.017.0280710-11
CAGAGTA339500.016.47756410-11
ACCGAAC10550.015.4479478
ATGGGGG142200.014.956785
GTACTTT230550.014.73958314-15
Produced by FastQC (version 0.11.5)