##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10839018
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.494448482325616	34.0	31.0	34.0	31.0	34.0
2	32.696741992678675	34.0	31.0	34.0	31.0	34.0
3	32.80384449956629	34.0	31.0	34.0	31.0	34.0
4	36.25895260991356	37.0	37.0	37.0	35.0	37.0
5	36.10796577697352	37.0	35.0	37.0	35.0	37.0
6	36.15241611371067	37.0	36.0	37.0	35.0	37.0
7	36.14320208712634	37.0	36.0	37.0	35.0	37.0
8	36.11154276153061	37.0	36.0	37.0	35.0	37.0
9	37.98525207726382	39.0	38.0	39.0	35.0	39.0
10-11	37.87087700195718	39.0	38.0	39.0	35.0	39.0
12-13	37.894866721321065	39.0	38.0	39.0	35.0	39.0
14-15	39.26656100211292	40.0	39.0	41.0	36.0	41.0
16-17	39.244034653323766	40.0	39.0	41.0	36.0	41.0
18-19	39.282555255466875	40.0	39.0	41.0	36.0	41.0
20-21	39.28209025198111	40.0	39.0	41.0	36.0	41.0
22-23	39.245933170420884	40.0	39.0	41.0	36.0	41.0
24-25	39.25397214865726	40.0	39.0	41.0	36.0	41.0
26-27	39.13493011395704	40.0	39.0	41.0	36.0	41.0
28-29	39.05396172307765	40.0	39.0	41.0	36.0	41.0
30-31	38.95990741869956	40.0	38.5	41.0	35.0	41.0
32-33	38.877700097096955	40.0	38.0	41.0	35.0	41.0
34-35	38.81922165390043	40.0	38.0	41.0	35.0	41.0
36-37	38.79272395588761	40.0	38.0	41.0	35.0	41.0
38-39	38.73445617876963	40.0	38.0	41.0	35.0	41.0
40-41	38.71505438077136	40.0	38.0	41.0	35.0	41.0
42-43	38.69358213705649	40.0	38.0	41.0	35.0	41.0
44-45	38.63744209909012	40.0	38.0	41.0	35.0	41.0
46-47	38.567973943016284	40.0	38.0	41.0	35.0	41.0
48-49	38.54056820014071	40.0	38.0	41.0	34.0	41.0
50-51	38.47997040775921	40.0	38.0	41.0	34.0	41.0
52-53	38.32434460029592	40.0	38.0	41.0	34.0	41.0
54-55	38.19061212342222	40.0	37.0	41.0	34.0	41.0
56-57	38.05775467508775	40.0	37.0	41.0	34.0	41.0
58-59	37.910062479909	39.5	37.0	41.0	33.0	41.0
60-61	37.79002359498456	39.0	36.0	41.0	33.0	41.0
62-63	37.597666377325794	39.0	36.0	41.0	33.0	41.0
64-65	37.3803338467327	39.0	35.5	40.5	33.0	41.0
66-67	37.15627411117683	38.5	35.0	40.0	33.0	41.0
68-69	36.88582248894219	38.0	35.0	40.0	32.0	41.0
70-71	36.58478624649884	37.0	35.0	40.0	32.0	41.0
72-73	36.270124741065146	37.0	35.0	39.0	32.0	41.0
74-75	35.87203627048775	36.0	35.0	39.0	31.5	40.5
76-77	33.882552975646185	34.5	32.0	36.5	29.0	39.0
78-79	34.99814301900811	35.0	34.0	37.0	31.0	39.0
80-81	34.95956924784882	35.0	34.0	37.0	31.0	39.0
82-83	34.6845054830007	35.0	34.0	36.5	31.0	39.0
84-85	34.36565306006894	35.0	34.0	36.0	31.0	37.5
86-87	34.17957040502864	35.0	34.0	36.0	31.0	37.0
88-89	33.98867371371796	35.0	34.0	35.5	31.0	37.0
90-91	33.81759626390213	35.0	34.0	35.0	30.0	36.0
92-93	33.73892600739749	35.0	34.0	35.0	30.5	36.0
94-95	33.666212318520195	35.0	34.0	35.0	30.0	36.0
96-97	33.680402116152266	35.0	34.0	35.0	31.0	36.0
98-99	33.710366380037314	35.0	34.0	35.0	31.0	35.0
100-101	32.472171490706515	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	6.0
20	44.0
21	159.0
22	666.0
23	2056.0
24	5395.0
25	12386.0
26	22912.0
27	38952.0
28	59440.0
29	85024.0
30	117606.0
31	157184.0
32	211870.0
33	293583.0
34	439134.0
35	683457.0
36	1257834.0
37	2507710.0
38	3347370.0
39	1568029.0
40	28197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15975100327354	20.228963546328643	13.226631785277965	24.38465366511985
2	14.737838038567869	21.781347868747645	40.815282851492526	22.665531241191957
3	18.045822970309672	26.61250308837941	30.655544625906145	24.686129315404774
4	12.182595182810246	18.120063554081447	39.641696843414806	30.0556444196935
5	12.718993020288705	38.28737870061104	36.18841871477473	12.805209564325523
6	29.359687372537486	38.566548429735626	19.263110861722943	12.810653336003943
7	25.98333170034407	32.82719892152592	23.96968064819156	17.21978872993845
8	24.24446568868139	36.71677637217689	21.938269684578437	17.10048825456328
9	25.719673129060215	16.260356796159947	21.496024824389075	36.52394525039077
10-11	23.887213609023352	26.846771169071644	29.711628902521774	19.554386319383227
12-13	24.642822232435375	24.51931413565252	29.57843624467667	21.259427387235434
14-15	21.717917013869155	25.958689423467963	27.085572158948747	25.23782140371414
16-17	21.418430156680245	28.956327039958786	27.80100558925172	21.82423721410925
18-19	21.395926772824122	28.388735406209825	29.254768899739314	20.960568921226734
20-21	22.27505334100289	27.625207483365234	29.021080785064786	21.07865839056709
22-23	21.887917510263883	27.637315112187128	28.845612989985348	21.62915438756365
24-25	21.60431050117052	27.705233118611233	29.00458101502224	21.685875365196008
26-27	21.619192371906845	27.89010018391026	28.863154255817165	21.627553188365734
28-29	21.57759938595947	28.0157932836175	28.81962841251514	21.58697891790789
30-31	21.889654192143873	27.84657121738005	28.712193940752055	21.55158064972402
32-33	21.592655758230666	28.032622238947063	28.704722630623415	21.669999372198856
34-35	21.73709964236272	28.033143417364098	28.737581338448393	21.492175601824794
36-37	21.735418034695332	27.979899597645037	28.666896974179533	21.617785393480105
38-39	21.559181714132688	28.138586966198666	28.71606589571031	21.586165423958334
40-41	21.835465597477448	27.98648474985847	28.575069438620453	21.602980214043626
42-43	21.68440019260939	28.100933534227003	28.422355309961322	21.792310963202286
44-45	21.832065977019415	28.18105681033829	28.25139239434804	21.73548481829425
46-47	21.856319791800882	28.15206729133316	28.049108615618685	21.942504301247272
48-49	21.80174870038659	28.217378057690105	28.074268650510398	21.906604591412908
50-51	21.78572372636606	28.345579639479514	27.97306762079797	21.89562901335646
52-53	21.960198912092537	28.225364558943188	27.848144131922137	21.966292397042146
54-55	21.919131726356454	28.158719724800264	27.950524321228066	21.971624227615212
56-57	21.90067453724319	28.28538048656698	27.87738627059483	21.936558705595004
58-59	21.879952813792837	28.323168674726734	27.862071435232032	21.934807076248394
60-61	21.968125120274312	28.282214928010358	27.86550270289183	21.8841572488235
62-63	21.936200950826024	28.292117265330287	27.838881664737038	21.932800119106656
64-65	21.90524354678568	28.271212880065228	27.844337732826517	21.97920584032257
66-67	21.98781141127615	28.306854204414783	27.651802866979914	22.053531517329155
68-69	21.937930385993898	28.304259714457626	27.739007183844226	22.018802715704254
70-71	21.915950374982838	28.09807834098524	27.826897467234435	22.159073816797495
72-73	21.95203540703333	28.131736262520494	27.79701757661867	22.119210753827506
74-75	21.840307994732303	28.240826533716344	27.766726528325425	22.15213894322593
76-77	21.920747165948665	28.19261610759637	27.69451076257125	22.192125963883715
78-79	21.94071900123843	28.16162758393797	27.69188847536296	22.20576493946064
80-81	21.927322964588992	28.225803361158814	27.645645283804026	22.201228390448165
82-83	21.9719289997553	28.181845256638947	27.762456862693952	22.083768880911794
84-85	22.14874070661735	28.11076553986862	27.6598047734553	22.080688980058735
86-87	21.90914724052775	28.174450450067173	27.786676131398526	22.129726178006553
88-89	22.025202523853167	28.126983762884123	27.72613029808054	22.121683415182165
90-91	22.024270414490555	28.19741614693273	27.719361706483518	22.058951732093192
92-93	22.049389703918028	28.121215431255166	27.764868609910188	22.06452625491662
94-95	21.95420752714764	28.194551427328534	27.787392100356538	22.063848945167287
96-97	21.93741080365029	28.19875903658737	27.839749213947375	22.02408094581497
98-99	22.22888295951366	28.697422847171765	27.854078435644425	21.219615757670148
100-101	26.121869942601162	35.79878787902179	28.580943516142714	9.498398662234335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1937.0
1	1831.5
2	2669.0
3	3914.0
4	5386.0
5	8851.5
6	11883.0
7	13672.5
8	14561.0
9	14068.0
10	12783.5
11	11559.5
12	12426.0
13	16180.0
14	24434.5
15	35636.5
16	45271.5
17	51532.5
18	54021.0
19	53388.5
20	51505.0
21	50143.0
22	51722.5
23	58690.5
24	70829.5
25	88138.5
26	111303.0
27	137095.5
28	164279.0
29	193349.5
30	220843.0
31	246923.5
32	273595.5
33	298733.5
34	322984.0
35	346796.5
36	371830.0
37	394197.5
38	411230.5
39	426786.5
40	441160.5
41	452598.0
42	460110.5
43	466969.0
44	474433.0
45	480270.5
46	481124.5
47	478894.5
48	473704.0
49	463181.5
50	450734.0
51	432615.0
52	409301.5
53	387730.0
54	365816.5
55	338739.0
56	308994.0
57	278335.0
58	245594.0
59	212697.5
60	179410.0
61	147076.0
62	117285.5
63	91816.5
64	70324.5
65	53252.0
66	40715.5
67	31488.0
68	24241.0
69	17459.5
70	12289.5
71	8762.0
72	6017.0
73	4040.0
74	2752.0
75	1887.0
76	1281.0
77	881.5
78	609.0
79	414.0
80	282.5
81	186.5
82	135.5
83	97.0
84	57.5
85	34.0
86	27.0
87	23.5
88	16.0
89	9.5
90	6.0
91	4.5
92	3.5
93	2.0
94	2.5
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001872863390392008
3	0.0
4	0.0034597230118078963
5	2.8600376897611944E-4
6	4.3361861747992297E-4
7	0.0
8	0.0
9	0.0
10-11	3.044556250390949E-4
12-13	4.843612216531055E-4
14-15	1.8451856062975448E-5
16-17	0.0
18-19	0.0010932724717312953
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.0783508748036474E-5
28-29	0.0
30-31	4.166943691256511E-5
32-33	9.944345587013094E-4
34-35	6.76993889406465E-4
36-37	3.43156703213639E-4
38-39	0.0013959352360120524
40-41	4.800540425638957E-6
42-43	9.708133697699007E-6
44-45	8.848472126944115E-5
46-47	2.4931869925647677E-5
48-49	3.033584817514705E-5
50-51	1.1270222749805256E-4
52-53	1.0371888200172465E-5
54-55	0.002793081683673839
56-57	0.0025695489930799334
58-59	0.0014183921344061913
60-61	1.3667516877333217E-4
62-63	2.2155250592223696E-5
64-65	9.537721238664979E-5
66-67	4.5496764383858936E-5
68-69	1.6152518541072219E-4
70-71	0.0
72-73	0.0
74-75	1.5041602967985032E-4
76-77	1.8302599811092764E-5
78-79	2.4754970658861586E-4
80-81	0.0
82-83	9.566041013571788E-5
84-85	3.8820136988499406E-5
86-87	0.0015559840092114253
88-89	0.0
90-91	1.0168476056594483E-4
92-93	6.895922530654617E-6
94-95	1.1231581522504053E-4
96-97	3.58200161677225E-5
98-99	7.466263687527905E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	367.0
20-21	816.0
22-23	1691.0
24-25	4461.0
26-27	7039.0
28-29	16697.0
30-31	37345.0
32-33	47404.0
34-35	63747.0
36-37	99647.0
38-39	116312.0
40-41	113345.0
42-43	124353.0
44-45	142295.0
46-47	141027.0
48-49	131251.0
50-51	121205.0
52-53	116279.0
54-55	123455.0
56-57	127498.0
58-59	126053.0
60-61	120791.0
62-63	115068.0
64-65	118028.0
66-67	124304.0
68-69	124838.0
70-71	120909.0
72-73	114209.0
74-75	114994.0
76-77	114592.0
78-79	119800.0
80-81	120203.0
82-83	113284.0
84-85	111339.0
86-87	116134.0
88-89	121455.0
90-91	124922.0
92-93	126492.0
94-95	135502.0
96-97	201525.0
98-99	828256.0
100-101	5990086.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.62471803852483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.19894019489504	77.23258821561653
2	6.817785611274581	11.675419412249953
3	1.6568944432493187	4.256133585684645
4	0.6125224718043457	2.097882557620295
5	0.28969773958689393	1.2402643634262893
6	0.1402429889790919	0.7204959832928814
7	0.08903387389463124	0.5336450243673764
8	0.050378045780679906	0.345088477224209
9	0.03280238983532481	0.2527825842575514
>10	0.109503623657285	1.4199623399279737
>50	0.0015886728127338283	0.08796347521698893
>100	5.084975870038402E-4	0.06815633317885823
>500	6.763639503032548E-5	0.03437110953415647
>1k	3.381024803070949E-5	0.0352465384023691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.225928031487724E-6	0.0	0.0	0.0
3	0.0	9.225928031487724E-6	0.0	9.225928031487724E-6	0.0
4	0.0	5.535556818892634E-5	0.0	9.225928031487724E-6	0.0
5	0.0	5.535556818892634E-5	0.0	9.225928031487724E-6	0.0
6	9.225928031487724E-6	5.535556818892634E-5	1.8451856062975448E-5	9.225928031487724E-6	0.0
7	9.225928031487724E-6	5.535556818892634E-5	1.8451856062975448E-5	2.767778409446317E-5	0.0
8	9.225928031487724E-6	6.458149622041407E-5	1.8451856062975448E-5	2.767778409446317E-5	0.0
9	9.225928031487724E-6	1.1071113637785269E-4	1.8451856062975448E-5	2.767778409446317E-5	0.0
10-11	1.8451856062975448E-5	1.1071113637785269E-4	2.306482007871931E-5	2.767778409446317E-5	0.0
12-13	1.8451856062975448E-5	1.199370644093404E-4	2.767778409446317E-5	3.2290748110207034E-5	0.0
14-15	1.8451856062975448E-5	1.9835745267698606E-4	2.767778409446317E-5	3.6903712125950896E-5	9.225928031487724E-6
16-17	1.8451856062975448E-5	2.0297041669272993E-4	3.2290748110207034E-5	3.6903712125950896E-5	9.225928031487724E-6
18-19	1.8451856062975448E-5	2.306482007871931E-4	3.6903712125950896E-5	5.535556818892634E-5	9.225928031487724E-6
20-21	1.8451856062975448E-5	2.767778409446317E-4	3.6903712125950896E-5	8.303335228338951E-5	9.225928031487724E-6
22-23	1.8451856062975448E-5	2.9061673299186333E-4	3.6903712125950896E-5	1.2455002842508427E-4	1.3838892047231586E-5
24-25	1.8451856062975448E-5	3.136815530705826E-4	4.612964015743862E-5	2.0297041669272993E-4	1.8451856062975448E-5
26-27	2.767778409446317E-5	3.2290748110207034E-4	4.612964015743862E-5	2.8600376897611944E-4	1.8451856062975448E-5
28-29	2.767778409446317E-5	3.2290748110207034E-4	5.074260417318248E-5	7.795909186607125E-4	1.8451856062975448E-5
30-31	2.767778409446317E-5	4.01327869369716E-4	5.535556818892634E-5	0.002403354252202552	1.8451856062975448E-5
32-33	3.6903712125950896E-5	4.0594083338545985E-4	5.535556818892634E-5	0.005309521582121185	1.8451856062975448E-5
34-35	3.6903712125950896E-5	4.6590936559013003E-4	5.535556818892634E-5	0.008760018665897594	1.8451856062975448E-5
36-37	3.6903712125950896E-5	5.258778977948002E-4	5.535556818892634E-5	0.0137143420188065	1.8451856062975448E-5
38-39	3.6903712125950896E-5	5.35103825826288E-4	5.535556818892634E-5	0.021740899406200817	2.306482007871931E-5
40-41	3.6903712125950896E-5	6.089112500781898E-4	7.380742425190179E-5	0.03101295707784598	2.767778409446317E-5
42-43	3.6903712125950896E-5	6.227501421254213E-4	7.380742425190179E-5	0.0388918996167365	2.767778409446317E-5
44-45	4.151667614169476E-5	6.734927462986038E-4	8.303335228338951E-5	0.04773956459893322	3.6903712125950896E-5
46-47	4.612964015743862E-5	7.196223864560425E-4	8.303335228338951E-5	0.05715923711908219	4.612964015743862E-5
48-49	5.535556818892634E-5	7.242353504717863E-4	9.225928031487724E-5	0.0659100298569483	4.612964015743862E-5
50-51	5.535556818892634E-5	8.164946307866635E-4	9.225928031487724E-5	0.07474846891111353	4.612964015743862E-5
52-53	5.535556818892634E-5	8.303335228338951E-4	1.0148520834636496E-4	0.08272428369433468	5.9968532204670205E-5
54-55	6.919446023615793E-5	8.303335228338951E-4	1.199370644093404E-4	0.09133668751172846	7.380742425190179E-5
56-57	8.303335228338951E-5	8.303335228338951E-4	1.199370644093404E-4	0.09985221908479164	7.380742425190179E-5
58-59	8.303335228338951E-5	8.303335228338951E-4	1.199370644093404E-4	0.1082847173055714	7.842038826764565E-5
60-61	8.303335228338951E-5	8.533983429126144E-4	1.199370644093404E-4	0.11578078383115518	8.303335228338951E-5
62-63	8.303335228338951E-5	8.810761270070775E-4	1.199370644093404E-4	0.1227140687468182	9.225928031487724E-5
64-65	8.303335228338951E-5	0.0010056261554321618	1.3838892047231586E-4	0.12981342036704802	9.225928031487724E-5
66-67	8.303335228338951E-5	0.0010286909755108811	1.3838892047231586E-4	0.1370050312675927	9.225928031487724E-5
68-69	8.303335228338951E-5	0.0010379169035423688	1.3838892047231586E-4	0.1439659939673502	9.225928031487724E-5
70-71	8.303335228338951E-5	0.0010563687596053441	1.3838892047231586E-4	0.15106534558757997	9.225928031487724E-5
72-73	8.303335228338951E-5	0.001060981723621088	1.3838892047231586E-4	0.15714984512434613	1.1071113637785269E-4
74-75	1.0148520834636496E-4	0.0010794335796840636	1.3838892047231586E-4	0.1626254334110341	1.1071113637785269E-4
76-77	1.2455002842508427E-4	0.0011117243277942706	1.3838892047231586E-4	0.16697084551386482	1.1071113637785269E-4
78-79	1.2916299244082813E-4	0.0011255632198415022	1.3838892047231586E-4	0.1709010908552786	1.1071113637785269E-4
80-81	1.2916299244082813E-4	0.0011255632198415022	1.3838892047231586E-4	0.1726263393971668	1.1532410039359655E-4
82-83	1.2916299244082813E-4	0.001130176183857246	1.3838892047231586E-4	0.1731752821150403	1.3838892047231586E-4
84-85	1.2916299244082813E-4	0.0011347891478729899	1.3838892047231586E-4	0.17383954893330744	1.3838892047231586E-4
86-87	1.33775956456572E-4	0.0011347891478729899	1.3838892047231586E-4	0.17414861752236227	1.3838892047231586E-4
88-89	1.3838892047231586E-4	0.0011716928599989408	1.4300188448805972E-4	0.17424087680267714	1.4761484850380358E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10780	0.0	45.584988	1
GTATCAA	19115	0.0	34.085327	1
TCAACGC	22455	0.0	28.180302	4
ATCAACG	22525	0.0	28.128778	3
CAACGCA	23130	0.0	27.356659	5
AACGCAG	23895	0.0	26.514936	6
TATCAAC	24665	0.0	25.936525	2
ACGCAGA	28330	0.0	22.4214	7
CGCAGAG	28535	0.0	22.246042	8
GTACATG	30170	0.0	21.703703	1
TACATGG	29575	0.0	21.506582	2
ACATGGG	30010	0.0	20.895746	3
CATGGGG	21385	0.0	19.187017	4
GCAGAGT	34950	0.0	18.092882	9
GAGTACT	19720	0.0	17.190308	12-13
AGAGTAC	31010	0.0	17.02807	10-11
CAGAGTA	33950	0.0	16.477564	10-11
ACCGAAC	1055	0.0	15.447947	8
ATGGGGG	14220	0.0	14.95678	5
GTACTTT	23055	0.0	14.739583	14-15
>>END_MODULE