FastQCFastQC Report
Fri 27 May 2016
SRR2139941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23154128
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA285550.050.465341
GTATCAA520450.035.1952631
TCAACGC657150.027.3002534
ATCAACG663650.027.0328663
CAACGCA673300.026.6210385
AACGCAG687000.026.161686
TATCAAC690850.026.1463322
ACGCAGA803850.022.3129527
CGCAGAG813950.022.00598
GTACATG757800.021.272031
TACATGG751900.021.0191122
ACATGGG754200.020.3782353
GCAGAGT942250.018.8619759
CATGGGG514250.018.4304334
GTGGTAT136750.018.3773631
GAGTACT549050.017.6990612-13
AGAGTAC869600.017.6769110-11
TGGTATC134450.017.1366372
CAGAGTA932050.017.05038510-11
GTACTTT619000.015.66358514-15
Produced by FastQC (version 0.11.5)