##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23154128
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60460916515621	31.0	31.0	34.0	30.0	34.0
2	31.740790583864786	31.0	31.0	34.0	30.0	34.0
3	31.782708163313256	31.0	31.0	34.0	30.0	34.0
4	35.5486649292083	37.0	35.0	37.0	33.0	37.0
5	35.39460134279296	37.0	35.0	37.0	33.0	37.0
6	35.420056760505084	37.0	35.0	37.0	33.0	37.0
7	35.318667237220076	37.0	35.0	37.0	32.0	37.0
8	35.30323253805974	37.0	35.0	37.0	32.0	37.0
9	36.90077704502627	39.0	37.0	39.0	33.0	39.0
10-11	36.78328920441314	39.0	37.0	39.0	32.0	39.0
12-13	36.707565234156085	39.0	35.0	39.0	32.0	39.0
14-15	37.84540076395881	40.0	37.0	41.0	32.0	41.0
16-17	37.814787691421586	40.0	37.0	41.0	32.0	41.0
18-19	37.83861363727453	40.0	37.0	41.0	32.0	41.0
20-21	37.82248806622204	40.0	37.0	41.0	32.0	41.0
22-23	37.725305551127065	40.0	37.0	41.0	32.0	41.0
24-25	37.61769125842468	39.0	36.0	41.0	32.0	41.0
26-27	37.42407643263081	39.0	36.0	41.0	31.5	41.0
28-29	37.28454677504006	39.0	36.0	41.0	31.0	41.0
30-31	37.100733447695596	39.0	36.0	40.5	31.0	41.0
32-33	36.894954352808696	39.0	35.5	40.0	30.0	41.0
34-35	36.76052499724049	39.0	35.0	40.0	30.0	41.0
36-37	36.601330371781174	38.0	35.0	40.0	30.0	41.0
38-39	36.436738942522936	38.0	35.0	40.0	30.0	41.0
40-41	36.317023272955225	38.0	35.0	40.0	30.0	41.0
42-43	36.123603074278805	38.0	34.5	40.0	29.5	41.0
44-45	35.95687041136282	38.0	34.0	40.0	29.0	41.0
46-47	36.10109608998185	38.0	34.5	40.0	29.0	41.0
48-49	36.507657654562614	38.5	35.0	40.0	30.0	41.0
50-51	36.59314720593691	39.0	35.0	40.0	30.0	41.0
52-53	36.554897137360435	39.0	35.0	40.0	30.0	41.0
54-55	36.444392926850526	38.0	35.0	40.0	30.0	41.0
56-57	36.29854266887199	38.0	34.5	40.0	29.0	41.0
58-59	36.098695798082204	38.0	34.0	40.0	29.0	41.0
60-61	35.92690687539462	38.0	34.0	40.0	28.0	41.0
62-63	35.715692558880626	37.5	34.0	40.0	28.0	41.0
64-65	35.48633698647133	37.0	34.0	40.0	28.0	41.0
66-67	35.237399935753984	37.0	33.5	39.5	28.0	41.0
68-69	34.96960724409091	36.0	33.0	39.0	28.0	41.0
70-71	34.694526638453645	36.0	33.0	39.0	28.0	40.5
72-73	34.39211816466664	35.0	32.5	38.5	27.5	40.0
74-75	34.08080631432902	35.0	32.0	38.0	27.0	40.0
76-77	32.30189331307434	33.5	30.0	36.0	25.5	38.5
78-79	33.36915999219459	35.0	31.5	36.5	26.5	39.0
80-81	33.40089677493842	35.0	32.0	36.0	27.0	39.0
82-83	33.17705615897506	35.0	32.0	36.0	27.0	37.5
84-85	32.975553232396464	34.5	32.0	35.5	27.0	37.0
86-87	32.776367164732406	34.0	32.0	35.0	26.5	37.0
88-89	32.631723506588415	34.0	32.0	35.0	26.0	36.0
90-91	32.51572166057778	34.0	31.5	35.0	26.0	36.0
92-93	32.43780320702034	34.0	31.0	35.0	27.0	36.0
94-95	32.411877986076284	34.0	31.0	35.0	27.0	35.5
96-97	32.507837811889154	34.0	32.0	35.0	27.0	35.0
98-99	32.671610294831424	34.0	32.0	35.0	28.5	35.0
100	31.28682930177226	33.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.0
18	3.0
19	58.0
20	334.0
21	2010.0
22	9227.0
23	29932.0
24	75025.0
25	150746.0
26	255368.0
27	384407.0
28	521564.0
29	663547.0
30	802900.0
31	957123.0
32	1135242.0
33	1369851.0
34	1697127.0
35	2161335.0
36	2913286.0
37	3973537.0
38	4053455.0
39	1931566.0
40	66479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.166284819709034	21.482247139689303	14.723443698678699	23.62802434192296
2	14.592710574933745	22.12372250399596	41.36319289238242	21.920374028687874
3	17.71152426902019	27.464294919679116	31.427307476230588	23.3968733350701
4	12.342767916947988	19.058075710148398	39.174483759204485	29.424672613699133
5	12.311973052926026	38.82891638156272	36.29423660437568	12.56487396113557
6	28.729948283951785	39.45084004027273	18.955177236646527	12.864034439128954
7	25.350120088742713	33.37022380502621	23.893865659456733	17.385790446774347
8	24.00605196619799	37.49025227812509	21.520335380369325	16.98336037530759
9	25.417607122084846	16.433162496919014	21.283694706760382	36.86553567423576
10-11	23.729217997548513	27.126843878984936	29.58357196988232	19.56036615358423
12-13	24.6052214806504	24.710468399535944	29.509225182453473	21.175084937360182
14-15	21.79922931417729	26.093352340805136	26.922643455179173	25.184774889838398
16-17	21.34801477919817	29.2667223221352	27.5586411787684	21.82662171989823
18-19	21.383643615671623	28.297544968981125	29.240949140513912	21.077862274833336
20-21	22.240107534166302	27.446078019672687	29.075237829525253	21.23857661663576
22-23	21.94549569913771	27.491239624222374	28.979878189574897	21.583386487065013
24-25	21.743418504557532	27.670505131848138	29.047532053321568	21.538544310272762
26-27	21.762137031429297	27.75422991758466	28.942701305556156	21.540931745429877
28-29	21.60178331583839	27.655182123285304	28.994376371359444	21.748658189516863
30-31	22.12118023461509	27.62821525927181	28.81166150450388	21.43894300160922
32-33	21.962293850043018	27.701705691822998	28.872308076652324	21.463692381481657
34-35	21.704327547343876	27.778154791273213	29.01298881650764	21.50452884487527
36-37	21.888217586065252	27.707423902756783	28.817265721969783	21.58709278920818
38-39	21.633818450270798	27.776344301292422	28.9134028009006	21.67643444753618
40-41	21.8489100178404	27.66488226915537	28.862539813683814	21.623667899320413
42-43	21.888161453534583	27.68788847778821	28.639153643850257	21.78479642482695
44-45	21.78533468477896	27.921488190865144	28.44891031137645	21.844266812979445
46-47	22.066159846535253	27.75051083703286	28.04239360097427	22.140935715457623
48-49	21.909673213503506	27.891658960468135	28.084193806837117	22.114474019191242
50-51	21.754552401947137	28.030486335839015	28.06023258758419	22.15472867462966
52-53	21.93787170404055	27.93035739600647	28.075715867481705	22.056055032471274
54-55	21.92770547999959	27.831417364709026	28.030627685534608	22.210249469756764
56-57	22.010785077598584	27.97293560137498	27.852915365736596	22.163363955289835
58-59	21.822452323177803	28.04246146081299	27.943545870948324	22.191540345060883
60-61	22.157423095137826	28.127752537863373	27.882734170684525	21.832090196314272
62-63	21.75359056902895	28.263362795632872	27.861430185087432	22.121616450250745
64-65	21.73950449873788	28.2050295131193	27.80875971082033	22.24670627732249
66-67	21.87108392394503	28.180157324663956	27.858811008024563	22.089947743366448
68-69	21.852573505115902	28.284781243873418	27.73795159474513	22.124693656265546
70-71	21.87453407141256	27.95169710101445	27.63127793658914	22.542490890983853
72-73	21.972037761340975	27.95606307174791	27.542574034440992	22.529325132470127
74-75	21.818235170713567	28.141315696924014	27.774051699558832	22.26639743280358
76-77	21.988299369133166	27.815055851870113	27.723560172214096	22.473084606782624
78-79	21.923195598905437	27.853913605517523	27.401379070531906	22.821511725045134
80-81	22.26785894630317	28.079871802128586	27.349198621724724	22.303070629843518
82-83	22.249078004352686	27.76355776842135	27.54380696818966	22.443557259036307
84-85	22.112091065068025	27.710933429828543	27.475094114440303	22.701881390663132
86-87	21.92835969921621	28.06190520094667	27.57173143309472	22.438003666742404
88-89	22.288516926713612	27.98192396118413	27.555673350863728	22.17388576123853
90-91	22.348064466629502	27.94094695274706	27.623583626662217	22.08740495396122
92-93	22.129459451270918	27.58527334886735	27.6382685729692	22.64699862689253
94-95	22.18057065854534	27.844843493355253	27.80965517250432	22.164930675595087
96-97	22.452332140935997	27.74643019888127	27.929299571056383	21.87193808912635
98-99	23.052759478618196	29.889078345244513	28.259905897821174	18.79825627831612
100	22.37212170812454	30.47709438142888	22.05998698079765	25.090796929648928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4043.0
1	3886.5
2	6357.5
3	9505.5
4	12625.0
5	20829.5
6	28152.0
7	33865.5
8	38054.0
9	37349.0
10	33506.0
11	29183.5
12	30386.5
13	39002.0
14	62196.5
15	96217.0
16	123158.0
17	137495.5
18	141859.0
19	138000.0
20	128593.0
21	117522.5
22	111040.5
23	116878.0
24	137895.5
25	173859.0
26	223132.5
27	279701.5
28	340483.0
29	412035.5
30	482796.5
31	548517.5
32	620450.0
33	690238.5
34	755918.0
35	810392.0
36	860141.0
37	900706.0
38	911890.5
39	911294.0
40	915796.0
41	910784.0
42	896065.0
43	891231.0
44	888289.0
45	885787.5
46	882000.0
47	875384.5
48	875006.0
49	881463.0
50	886482.0
51	867104.5
52	827685.0
53	791766.0
54	755395.5
55	711348.0
56	662353.0
57	606093.0
58	546428.0
59	484357.5
60	415010.0
61	345114.5
62	278868.0
63	220295.0
64	170559.0
65	130072.0
66	100472.0
67	78829.0
68	62154.0
69	47020.5
70	34700.5
71	25731.0
72	18978.0
73	14019.5
74	10342.0
75	7658.5
76	5576.0
77	3833.5
78	2656.0
79	1893.0
80	1335.5
81	961.0
82	743.5
83	570.5
84	428.0
85	313.5
86	234.0
87	188.5
88	137.5
89	94.5
90	73.0
91	55.0
92	40.0
93	21.5
94	7.5
95	3.0
96	1.5
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.13288343227609348
3	0.0
4	2.548141739563675E-4
5	0.0
6	0.0
7	4.318884304345213E-6
8	0.0
9	3.886995873910691E-5
10-11	8.205880178255903E-5
12-13	2.5913305826071272E-5
14-15	3.2607576497806353E-4
16-17	0.0015915088661512108
18-19	4.318884304345213E-6
20-21	4.6431144577816283E-4
22-23	0.0023651321207081356
24-25	0.0
26-27	0.004337085647517698
28-29	0.0033432159084105123
30-31	0.008899924650918071
32-33	0.009626936311387464
34-35	0.002980089983860759
36-37	0.007739539573867808
38-39	2.347495248038452E-4
40-41	2.6811870821746975E-5
42-43	0.002195654724832336
44-45	2.5105660023123223E-5
46-47	0.0
48-49	1.2869646972713892E-4
50-51	0.0
52-53	1.672022233500676E-5
54-55	0.0027490167261511283
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	2.229928973351719E-4
70-71	4.922869282386692E-4
72-73	4.831402018613745E-4
74-75	4.790258005759752E-4
76-77	0.0012104145847628349
78-79	0.007495825273045009
80-81	8.809278687394595E-4
82-83	4.564673884142936E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	3.483572129006839E-4
94-95	1.312627983279351E-4
96-97	4.0777321774050004E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	980.0
20-21	3065.0
22-23	6245.0
24-25	13637.0
26-27	16682.0
28-29	33137.0
30-31	77170.0
32-33	90814.0
34-35	120735.0
36-37	168094.0
38-39	193846.0
40-41	214472.0
42-43	241228.0
44-45	280826.0
46-47	264626.0
48-49	237569.0
50-51	210229.0
52-53	195136.0
54-55	210886.0
56-57	239409.0
58-59	157724.0
60-61	260506.0
62-63	251770.0
64-65	258941.0
66-67	280867.0
68-69	268393.0
70-71	267704.0
72-73	259027.0
74-75	261597.0
76-77	245410.0
78-79	271284.0
80-81	285583.0
82-83	274633.0
84-85	276892.0
86-87	293247.0
88-89	324535.0
90-91	356834.0
92-93	396098.0
94-95	462773.0
96-97	1017884.0
98-99	1537232.0
100-101	1.2326408E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.69352212728765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6480188270305	73.30616906413259
2	7.637842377298217	12.632001752636906
3	1.9554826769246532	4.851172500413894
4	0.7910241555994352	2.616502940571237
5	0.3666327652110423	1.5159077341283993
6	0.1996363421575409	0.9905179366569224
7	0.11901949305053135	0.6889498757506871
8	0.0730209102023805	0.4830685002860189
9	0.049575870278011795	0.36896429932328795
>10	0.15367083689325667	1.9566340740666284
>50	0.004315791623196335	0.2412838023256721
>100	0.0016581774012765149	0.2335274488042971
>500	4.076483411124997E-5	0.026218231549633514
>1k	6.1011495870342227E-5	0.08908183935381736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.159442152172606E-5	0.0	4.318884304345213E-6	0.0
3	0.0	2.5913305826071272E-5	0.0	8.637768608690425E-6	0.0
4	0.0	6.91021488695234E-5	0.0	2.5913305826071272E-5	4.318884304345213E-6
5	0.0	7.342103317386861E-5	0.0	3.0232190130416485E-5	4.318884304345213E-6
6	3.45510744347617E-5	7.773991747821383E-5	0.0	3.0232190130416485E-5	8.637768608690425E-6
7	3.45510744347617E-5	7.773991747821383E-5	0.0	3.0232190130416485E-5	8.637768608690425E-6
8	3.45510744347617E-5	1.0365322330428509E-4	0.0	3.0232190130416485E-5	1.2956652913035636E-5
9	3.45510744347617E-5	2.0730644660857018E-4	0.0	4.318884304345212E-5	2.159442152172606E-5
10-11	3.8869958739106913E-5	2.0730644660857018E-4	0.0	5.1826611652142544E-5	2.159442152172606E-5
12-13	3.8869958739106913E-5	2.0730644660857018E-4	0.0	6.69427067173508E-5	2.159442152172606E-5
14-15	4.318884304345212E-5	2.85046364086784E-4	0.0	7.342103317386861E-5	5.182661165214255E-5
16-17	8.205880178255903E-5	2.958435748476471E-4	0.0	8.421824393473164E-5	6.91021488695234E-5
18-19	8.421824393473163E-5	3.3039464928240873E-4	0.0	1.058126654564577E-4	7.773991747821383E-5
20-21	8.637768608690424E-5	3.6926460802151563E-4	0.0	1.7923369863032633E-4	9.501545469559466E-5
22-23	8.853712823907685E-5	4.1893177752148555E-4	0.0	5.549766331083598E-4	1.1660987621732073E-4
24-25	9.069657039124945E-5	4.7939615778231856E-4	0.0	0.0019089468625205837	1.4684206634773723E-4
26-27	9.069657039124945E-5	5.096283479127351E-4	0.0	0.004819874883649256	1.5116095065208243E-4
28-29	9.069657039124945E-5	5.139472322170803E-4	0.0	0.017271218333076502	1.5547983495642765E-4
30-31	9.285601254342205E-5	5.614549595648776E-4	0.0	0.021292099620421896	1.7059593002163588E-4
32-33	9.501545469559466E-5	5.70092728173568E-4	0.0	0.02958867636906905	2.116253309129154E-4
34-35	9.933433899993988E-5	6.456732034996092E-4	0.0	0.039686227872628155	2.2890086813029625E-4
36-37	1.058126654564577E-4	7.23413120977823E-4	0.0	0.057603119409204276	2.2890086813029625E-4
38-39	1.079721076086303E-4	7.320508895865135E-4	0.0	0.10463576948352363	2.3106031028246885E-4
40-41	1.1229099191297551E-4	8.205880178255903E-4	0.0	0.15264664685277718	2.3753863673898668E-4
42-43	1.2092876052166594E-4	8.55139092260352E-4	0.0	0.18315524557867174	2.3969807889115928E-4
44-45	1.2308820267383855E-4	9.695895263255002E-4	0.0	0.22090661328295325	2.461764053476771E-4
46-47	1.2524764482601115E-4	0.0010451700016515413	0.0	0.2604092885726467	2.504952896520223E-4
48-49	1.2524764482601115E-4	0.0010473294438037139	0.0	0.2951935827598431	2.5913305826071276E-4
50-51	1.4900150849990983E-4	0.001129388245586273	0.0	0.3327678762076464	2.612925004128854E-4
52-53	1.5979871926077285E-4	0.0011358665720427909	0.0	0.41762747446157333	2.7208971117374835E-4
54-55	1.5979871926077285E-4	0.001140185456347136	0.0	0.5203348621032068	2.8288692193461143E-4
56-57	1.5979871926077285E-4	0.0011531421092601717	0.0	0.6198462753596249	2.9584357484764703E-4
58-59	1.6195816141294545E-4	0.0011531421092601717	0.0	0.8761677399382088	3.0232190130416485E-4
60-61	1.7491481432598108E-4	0.0011812148572384155	0.0	1.0940273803444467	3.0232190130416485E-4
62-63	1.9003090939118933E-4	0.0012265631424340402	0.0	1.1060511542477436	3.044813434563375E-4
64-65	1.9003090939118933E-4	0.0013561296715643967	0.0	1.1154058576509556	3.0664078560851005E-4
66-67	1.9219035154336193E-4	0.0013734052087817775	0.0	1.1233785180767768	3.152785542172005E-4
68-69	1.9434979369553455E-4	0.0014122751675208845	0.0	1.1310488566012937	3.368729757389265E-4
70-71	2.0298756230422495E-4	0.0014446667998034735	0.0	1.137144961796877	3.541485129563074E-4
72-73	2.3321975243464145E-4	0.0014489856841078186	0.0	1.142083605998896	3.7142405017368826E-4
74-75	2.3321975243464145E-4	0.0015051311800643066	0.0	1.1458669486495021	3.822212609345513E-4
76-77	2.3537919458681408E-4	0.0016087844033685914	0.0	1.149430028200587	3.843807030867239E-4
78-79	2.461764053476771E-4	0.0016454949199555258	0.0	1.1518421250845639	3.8654014523889653E-4
80-81	2.461764053476771E-4	0.0016454949199555258	0.0	1.1525914515113675	4.0381568245627733E-4
82-83	2.4833584749984973E-4	0.001649813804259871	0.0	1.153245762483476	4.3620731473886646E-4
84-85	2.504952896520223E-4	0.0016584515728685614	0.0	1.1535372871740193	4.3620731473886646E-4
86-87	2.526547318041949E-4	0.0016800459943902876	0.0	1.1536906075668236	4.3620731473886646E-4
88	2.6345194256505796E-4	0.0017318726060424302	0.0	1.1537078831040408	4.4052619904321166E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	28555	0.0	50.46534	1
GTATCAA	52045	0.0	35.195263	1
TCAACGC	65715	0.0	27.300253	4
ATCAACG	66365	0.0	27.032866	3
CAACGCA	67330	0.0	26.621038	5
AACGCAG	68700	0.0	26.16168	6
TATCAAC	69085	0.0	26.146332	2
ACGCAGA	80385	0.0	22.312952	7
CGCAGAG	81395	0.0	22.0059	8
GTACATG	75780	0.0	21.27203	1
TACATGG	75190	0.0	21.019112	2
ACATGGG	75420	0.0	20.378235	3
GCAGAGT	94225	0.0	18.861975	9
CATGGGG	51425	0.0	18.430433	4
GTGGTAT	13675	0.0	18.377363	1
GAGTACT	54905	0.0	17.69906	12-13
AGAGTAC	86960	0.0	17.67691	10-11
TGGTATC	13445	0.0	17.136637	2
CAGAGTA	93205	0.0	17.050385	10-11
GTACTTT	61900	0.0	15.663585	14-15
>>END_MODULE