FastQCFastQC Report
Fri 27 May 2016
SRR2139940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21845829
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271950.050.659281
ATCTCGT217500.040.00647494
GTATCAA487150.035.4414021
TATCTCG255550.030.22782194
TCAACGC608150.027.7668484
ATCAACG617900.027.3218883
CAACGCA625550.026.9944345
AACGCAG634750.026.682816
TATCAAC649700.026.2496032
CGCTATC385050.024.51973594
GTACATG688300.022.8347851
ACGCAGA742700.022.7875027
CGCAGAG753400.022.4359138
TACATGG697300.022.1468472
CTATCTC380600.022.08471594
CCGTCTT130100.021.83019390-91
GCCGTCT135600.021.67644390-91
ACATGGG698050.021.4513323
TATGCCG129550.020.92862586-87
ATGCCGT136500.020.74433788-89
Produced by FastQC (version 0.11.5)