##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21845829
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702224026380506	31.0	31.0	34.0	30.0	34.0
2	31.835390179058894	31.0	31.0	34.0	30.0	34.0
3	31.88156768964913	31.0	31.0	34.0	30.0	34.0
4	35.62699465421981	37.0	35.0	37.0	33.0	37.0
5	35.47996764050474	37.0	35.0	37.0	33.0	37.0
6	35.50168070985084	37.0	35.0	37.0	33.0	37.0
7	35.40459242814727	37.0	35.0	37.0	33.0	37.0
8	35.39274293504724	37.0	35.0	37.0	33.0	37.0
9	37.00267300453556	39.0	37.0	39.0	33.0	39.0
10-11	36.893614634628875	39.0	37.0	39.0	33.0	39.0
12-13	36.820294139444194	39.0	37.0	39.0	32.0	39.0
14-15	37.98793227302109	40.0	37.0	41.0	33.0	41.0
16-17	37.96331945104944	40.0	37.0	41.0	33.0	41.0
18-19	37.983772600252436	40.0	37.0	41.0	33.0	41.0
20-21	37.96488629011826	40.0	37.0	41.0	32.0	41.0
22-23	37.86958125832531	40.0	37.0	41.0	32.0	41.0
24-25	37.76444346488746	40.0	37.0	41.0	32.0	41.0
26-27	37.5805489118576	39.5	36.5	41.0	32.0	41.0
28-29	37.45406102779902	39.0	36.0	41.0	31.5	41.0
30-31	37.27636437101093	39.0	36.0	41.0	31.0	41.0
32-33	37.0765151314151	39.0	36.0	41.0	31.0	41.0
34-35	36.948937952159326	39.0	35.5	40.0	30.0	41.0
36-37	36.794069485134116	39.0	35.0	40.0	30.0	41.0
38-39	36.64395296129524	38.0	35.0	40.0	30.0	41.0
40-41	36.51934093424026	38.0	35.0	40.0	30.0	41.0
42-43	36.31627645979697	38.0	35.0	40.0	30.0	41.0
44-45	36.15146814514758	38.0	35.0	40.0	29.5	41.0
46-47	36.302392812694706	38.0	35.0	40.0	30.0	41.0
48-49	36.69641748757036	39.0	35.0	40.0	30.0	41.0
50-51	36.78079187738388	39.0	35.0	41.0	30.0	41.0
52-53	36.73890697410172	39.0	35.0	41.0	30.0	41.0
54-55	36.62649977405273	39.0	35.0	40.0	30.0	41.0
56-57	36.48283697379701	38.0	35.0	40.0	30.0	41.0
58-59	36.28862706599382	38.0	34.5	40.0	29.0	41.0
60-61	36.11567798224888	38.0	34.0	40.0	29.0	41.0
62-63	35.916577679927165	38.0	34.0	40.0	29.0	41.0
64-65	35.68771423749162	37.0	34.0	40.0	28.0	41.0
66-67	35.438192859970975	37.0	34.0	40.0	28.0	41.0
68-69	35.16317937650567	36.0	33.5	39.0	28.0	41.0
70-71	34.88317265832136	36.0	33.0	39.0	28.0	41.0
72-73	34.581901666861036	35.5	33.0	39.0	28.0	40.0
74-75	34.25657592723009	35.0	33.0	38.0	28.0	40.0
76-77	32.46183367469311	34.0	30.5	36.0	25.5	39.0
78-79	33.48808434391765	35.0	32.0	37.0	27.0	39.0
80-81	33.49983946542773	35.0	32.0	36.0	27.0	39.0
82-83	33.26938044162473	35.0	32.0	36.0	27.0	37.5
84-85	33.071688619122	35.0	32.0	35.5	27.0	37.0
86-87	32.86804527048585	34.5	32.0	35.0	27.0	37.0
88-89	32.7270108519958	34.0	32.0	35.0	27.0	36.0
90-91	32.62681965073425	34.0	32.0	35.0	27.0	36.0
92-93	32.562523673001735	34.0	32.0	35.0	27.0	36.0
94-95	32.51616315535023	34.0	32.0	35.0	27.0	35.5
96-97	32.5920251365421	34.0	32.0	35.0	27.0	35.0
98-99	32.739062207604675	34.0	32.0	35.0	29.0	35.0
100	31.411535818217057	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	44.0
20	228.0
21	1711.0
22	8048.0
23	26104.0
24	65735.0
25	133441.0
26	227420.0
27	343396.0
28	466744.0
29	595861.0
30	724098.0
31	864582.0
32	1029465.0
33	1250359.0
34	1556177.0
35	2000847.0
36	2746812.0
37	3849428.0
38	4039200.0
39	1839481.0
40	76641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.608722150118446	21.211568579063766	14.636423273293955	23.54328599752383
2	14.711380437499116	21.945943458205342	41.24192691625666	22.100749188038883
3	17.911423732191622	27.103695629952977	31.159453825258815	23.825426812596582
4	12.521259833652945	18.66825220604462	39.08150560751501	29.728982352787426
5	12.563446321950062	38.619687996276085	36.11356657602694	12.703299105746915
6	29.195403845740987	39.045828839912645	18.852115889033097	12.906651425313271
7	25.657648423412997	33.16881680251182	23.842198892978608	17.33133588109657
8	24.183595870863954	37.325605725468236	21.376936530996375	17.11386187267144
9	25.557294204814085	16.41933180632892	21.378243400500104	36.64513058835689
10-11	23.942338870177316	26.982601719684578	29.448459186118015	19.62660022402009
12-13	24.685474255563776	24.665667269085304	29.431799491957893	21.21705898339303
14-15	21.91213548033031	26.027197760811262	26.877321187457397	25.183345571401034
16-17	21.457355395242818	29.130038023858006	27.53622428789094	21.876382293008238
18-19	21.510150588020743	28.26111695102607	29.202383631327685	21.0263488296255
20-21	22.32578101028949	27.41030331253355	29.081762782719995	21.182152894456962
22-23	22.052982439946543	27.293632147822315	28.979706152382974	21.673679259848168
24-25	21.825140133160136	27.509502302772816	29.00239269079598	21.662964873271065
26-27	21.77926530077256	27.64363731605623	28.918773781918368	21.658323601252842
28-29	21.772938009435688	27.574166571424325	29.00468665697916	21.648208762160827
30-31	22.07178786637391	27.45873738620517	28.811289167798463	21.65818557962246
32-33	21.82312296888639	27.74685789171847	28.71758452081265	21.71243461858249
34-35	21.965098002233237	27.66699023770906	28.765628967778646	21.60228279227906
36-37	21.918541140919174	27.592967997461297	28.77730688002915	21.711183981590374
38-39	21.743299797108413	27.695289509789646	28.78784350848413	21.77356718461781
40-41	22.058772794439555	27.54143106875675	28.73604114213896	21.66375499466474
42-43	21.936494601207794	27.615803679973645	28.45446066094006	21.9932410578785
44-45	21.9623043767393	27.734119076131954	28.29472404510373	22.008852502025018
46-47	22.092117095193732	27.66768863843193	28.134335643995755	22.105858622378584
48-49	21.945615702500394	27.926318649472066	28.019720195730198	22.10834545229734
50-51	21.874938036657895	28.022082886397744	27.83388653221132	22.269092544733045
52-53	21.955888202721397	27.77448286029355	27.80183228028067	22.467796656704387
54-55	22.1192412138678	27.47520673834388	28.09094606791389	22.314605979874433
56-57	21.958298591333342	27.306915702083124	28.146094150408807	22.58869155617473
58-59	21.765463681503935	27.19472336611357	28.188287093261604	22.85152585912089
60-61	21.9609316137794	27.404744061300068	28.464134670360114	22.170189654560417
62-63	21.47141024979479	27.686277582903944	28.41998254904571	22.42232961825556
64-65	21.335763000675556	28.016277018363656	28.03873322108051	22.60922675988028
66-67	21.45295578123846	28.5548085944571	27.53796458090748	22.454271043396957
68-69	21.492661492668326	28.542435670614886	27.370653369506343	22.594249467210453
70-71	21.574625725867826	27.92282126239617	27.37308106810941	23.12947194362659
72-73	21.80970408526908	27.45922003194296	27.329204042115673	23.401871840672285
74-75	21.79334056333179	27.549441202635432	27.51539746872767	23.141820765305106
76-77	22.21367204311685	27.377966672557307	26.968031100823797	23.440330183502045
78-79	22.425225065233654	27.50450580063876	26.41724079212172	23.653028342005864
80-81	22.299415849934082	27.81999179504744	26.573806508986642	23.30678584603184
82-83	22.26095249266264	27.52516237698722	26.692275470742004	23.521609659608135
84-85	22.31245175383562	27.91307368353153	26.653814951785026	23.12065961084782
86-87	22.705002638420467	27.86108009633203	26.77132555721846	22.662591708029037
88-89	22.511967460525746	27.893538513486014	27.111756664086535	22.482737361901712
90-91	22.49683893629255	27.482520012565065	27.075618171764663	22.94502287937772
92-93	22.467315029226864	26.981627808274673	27.31220590606832	23.23885125643014
94-95	21.710796544611732	27.388893238528272	27.99682084061174	22.903489376248253
96-97	21.807057446112015	27.308321210131542	28.129723203739193	22.754898140017247
98-99	22.95776886371152	29.21577792970385	28.555346086516302	19.271107120068322
100	22.287276491622805	29.991606364628172	22.206587938584732	25.514529205164294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3986.0
1	3671.5
2	5787.5
3	8703.5
4	11669.5
5	19372.0
6	26389.0
7	31905.0
8	35847.5
9	35551.0
10	31974.5
11	27448.5
12	28289.5
13	35781.0
14	56201.0
15	86567.0
16	110941.0
17	124447.5
18	128643.5
19	124865.5
20	115496.0
21	104030.0
22	96146.0
23	99158.0
24	115193.0
25	142900.5
26	182858.0
27	231751.0
28	287490.0
29	355489.0
30	420976.0
31	479498.0
32	544096.5
33	605781.5
34	662508.5
35	710660.5
36	756575.5
37	793201.5
38	805454.0
39	806692.0
40	812328.0
41	811000.0
42	799139.5
43	798375.5
44	802491.5
45	805773.5
46	806358.0
47	806251.0
48	817500.0
49	817874.0
50	804069.0
51	790022.5
52	768177.0
53	740493.5
54	710683.5
55	673788.5
56	627929.5
57	573798.5
58	517196.0
59	459400.5
60	391925.0
61	324381.5
62	263003.5
63	207624.0
64	159486.5
65	120888.0
66	92764.5
67	72938.0
68	57394.0
69	43217.0
70	32044.0
71	24320.5
72	18615.5
73	13972.0
74	10237.0
75	7505.0
76	5509.0
77	3830.5
78	2656.0
79	1918.5
80	1374.5
81	993.5
82	740.0
83	531.5
84	388.5
85	295.5
86	227.0
87	171.5
88	119.5
89	86.0
90	68.5
91	54.5
92	42.5
93	25.0
94	11.5
95	6.5
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.14073624763793585
3	0.0
4	2.5634183989996446E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	3.204272998749556E-5
10-11	9.383942353480841E-5
12-13	9.155065710713016E-6
14-15	2.7465197132139045E-4
16-17	0.0015288959736890735
18-19	4.577532855356508E-6
20-21	4.806704666337446E-4
22-23	0.0023739509317391126
24-25	0.0
26-27	0.004555242546845273
28-29	0.0035382118510193135
30-31	0.00869959996538533
32-33	0.009812871239906831
34-35	0.0031126000035400915
36-37	0.007818492671219361
38-39	2.3331008165059603E-4
40-41	2.8163078682619425E-5
42-43	0.002148293684617522
44-45	1.67164386687716E-5
46-47	0.0
48-49	1.4629782741142894E-4
50-51	0.0
52-53	1.2345137045588468E-5
54-55	0.0028971228699992686
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	2.5626157869894303E-6
66-67	0.0
68-69	1.287437331032061E-4
70-71	4.499194883717909E-4
72-73	3.9659427499167153E-4
74-75	3.909914797488151E-4
76-77	0.0010254936047170045
78-79	0.007139645767360332
80-81	7.672652800024826E-4
82-83	4.723102878076344E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	4.000640991589986E-4
94-95	1.3027189176802797E-4
96-97	5.724709496249137E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	823.0
20-21	2645.0
22-23	5403.0
24-25	12381.0
26-27	14345.0
28-29	25508.0
30-31	57764.0
32-33	68251.0
34-35	92713.0
36-37	123305.0
38-39	98173.0
40-41	166148.0
42-43	188177.0
44-45	224795.0
46-47	211741.0
48-49	186194.0
50-51	106496.0
52-53	40625.0
54-55	42695.0
56-57	46171.0
58-59	129826.0
60-61	220134.0
62-63	214739.0
64-65	227389.0
66-67	247666.0
68-69	248040.0
70-71	249444.0
72-73	245735.0
74-75	251403.0
76-77	237674.0
78-79	261579.0
80-81	275801.0
82-83	264888.0
84-85	269502.0
86-87	286371.0
88-89	314939.0
90-91	346262.0
92-93	383279.0
94-95	447336.0
96-97	948476.0
98-99	1621806.0
100-101	1.2439187E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.97203343967998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7844141222383	71.08082515788597
2	7.955328083765238	12.883181832445281
3	2.2118531384398272	5.3729473886823245
4	0.8697473162102672	2.817008350889987
5	0.42924960422070113	1.7378606653464004
6	0.2512445206129282	1.2206267834763833
7	0.13655915648523861	0.7740230809792062
8	0.09138544444043345	0.5919732210504635
9	0.06407449050578663	0.466941760907851
>10	0.19973194029627514	2.4735530301442723
>50	0.004714899739113947	0.2577482329031407
>100	0.0016160134689038356	0.2262345599046775
>500	2.3227761147152996E-5	0.015196771024634712
>1k	5.804181585650628E-5	0.08187916435947584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3732598566069522E-5	0.0	4.577532855356508E-6	0.0
3	0.0	1.3732598566069522E-5	0.0	1.3732598566069522E-5	0.0
4	0.0	8.697312425177365E-5	0.0	1.3732598566069522E-5	0.0
5	0.0	1.0528325567319968E-4	0.0	1.3732598566069522E-5	4.577532855356508E-6
6	0.0	1.0528325567319968E-4	0.0	1.3732598566069522E-5	4.577532855356508E-6
7	0.0	1.0528325567319968E-4	0.0	2.7465197132139045E-5	4.577532855356508E-6
8	0.0	1.1443832138391269E-4	0.0	3.662026284285206E-5	1.3732598566069522E-5
9	0.0	2.0141144563568634E-4	0.0	8.239559139641713E-5	1.831013142142603E-5
10-11	4.577532855356508E-6	2.0141144563568634E-4	0.0	8.92618906794519E-5	1.831013142142603E-5
12-13	4.577532855356508E-6	2.0141144563568634E-4	0.0	1.0299448924552143E-4	1.831013142142603E-5
14-15	4.577532855356508E-6	3.0440593488120776E-4	0.0	1.1214955495623444E-4	3.662026284285206E-5
16-17	4.577532855356508E-6	3.2958236558566854E-4	0.0	1.2130462066694745E-4	5.95079271196346E-5
18-19	4.577532855356508E-6	3.6391386200084234E-4	0.0	1.6021364993747778E-4	7.095175925802587E-5
20-21	4.577532855356508E-6	4.2113302269279867E-4	0.0	2.220103434847906E-4	7.324052568570412E-5
22-23	4.577532855356508E-6	4.852184826677898E-4	0.0	4.371543876865464E-4	7.781805854106062E-5
24-25	4.577532855356508E-6	5.309938112213549E-4	0.0	0.0011489607466944835	8.697312425177365E-5
26-27	4.577532855356508E-6	5.630365412088505E-4	0.0	0.0032134280644602687	8.697312425177365E-5
28-29	4.577532855356508E-6	5.630365412088505E-4	0.0	0.011967959650329589	8.92618906794519E-5
30-31	4.577532855356508E-6	6.637422640266937E-4	0.0	0.01675148148417714	9.38394235348084E-5
32-33	4.577532855356508E-6	6.706085633097283E-4	0.0	0.02743315440215155	9.612818996248666E-5
34-35	4.577532855356508E-6	7.781805854106063E-4	0.0	0.041152020369655005	1.0986078852855618E-4
36-37	4.577532855356508E-6	8.857526075114842E-4	0.0	0.06694184047673357	1.0986078852855618E-4
38-39	4.577532855356508E-6	9.10929038215945E-4	0.0	0.21960027243644542	1.1214955495623444E-4
40-41	4.577532855356508E-6	0.001045966257448962	0.0	0.37863978519652425	1.2588215352230396E-4
42-43	4.577532855356508E-6	0.0010871640531471705	0.0	0.4295007527523904	1.3503721923301696E-4
44-45	6.866299283034761E-6	0.0012061799073864398	0.0	0.5018898573269982	1.4190351851605174E-4
46-47	9.155065710713016E-6	0.0012931530316382133	0.0	0.5758536332038486	1.4648105137140825E-4
48-49	9.155065710713016E-6	0.00129773056449357	0.0	0.6451071277725373	1.6021364993747776E-4
50-51	9.155065710713016E-6	0.0014785431122801519	0.0	0.7192425611314636	1.6021364993747776E-4
52-53	9.155065710713016E-6	0.001487698177990865	0.0	1.2328600576338853	1.876788470696168E-4
54-55	1.3732598566069522E-5	0.0015082970758399693	0.0	1.907540336418453	2.220103434847906E-4
56-57	1.3732598566069522E-5	0.0015380510393997865	0.0	2.6473634852676	2.2887664276782538E-4
58-59	1.3732598566069522E-5	0.001558649937248891	0.0	3.4207674151436414	2.2887664276782538E-4
60-61	1.3732598566069522E-5	0.0015815376015256735	0.0	3.6353415565049056	2.334541756231819E-4
62-63	1.3732598566069522E-5	0.0016021364993747777	0.0	3.6463917208177357	2.4718677418925143E-4
64-65	1.3732598566069522E-5	0.001785237813589038	0.0	3.6550112151843726	2.494755406169297E-4
66-67	1.3732598566069522E-5	0.0017989704121551076	0.0	3.661863781868841	2.517643070446079E-4
68-69	1.3732598566069522E-5	0.0018264356092872465	0.0	3.6682837716984786	2.5634183989996446E-4
70-71	1.3732598566069522E-5	0.0018539008064193856	0.0	3.6738180089206045	2.746519713213905E-4
72-73	1.3732598566069522E-5	0.001883654769979203	0.0	3.6783612102795455	3.204272998749555E-4
74-75	1.3732598566069522E-5	0.0019568952956649068	0.0	3.6821216535202215	3.204272998749555E-4
76-77	1.3732598566069522E-5	0.002039290887061324	0.0	3.6857333269430974	3.204272998749555E-4
78-79	1.3732598566069522E-5	0.002073622383476498	0.0	3.6880449810350524	3.204272998749555E-4
80-81	1.3732598566069522E-5	0.002080488682759533	0.0	3.6888643594161614	3.318711320133468E-4
82-83	1.3732598566069522E-5	0.002091932514897924	0.0	3.689493770183773	3.478924970070946E-4
84-85	1.3732598566069522E-5	0.002101087580608637	0.0	3.6898187750165032	3.478924970070946E-4
86-87	1.3732598566069522E-5	0.0021056651134639935	0.0	3.689981277432868	3.478924970070946E-4
88	1.3732598566069522E-5	0.0021834831720050542	0.0	3.68999958756429	3.478924970070946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27195	0.0	50.65928	1
ATCTCGT	21750	0.0	40.006474	94
GTATCAA	48715	0.0	35.441402	1
TATCTCG	25555	0.0	30.227821	94
TCAACGC	60815	0.0	27.766848	4
ATCAACG	61790	0.0	27.321888	3
CAACGCA	62555	0.0	26.994434	5
AACGCAG	63475	0.0	26.68281	6
TATCAAC	64970	0.0	26.249603	2
CGCTATC	38505	0.0	24.519735	94
GTACATG	68830	0.0	22.834785	1
ACGCAGA	74270	0.0	22.787502	7
CGCAGAG	75340	0.0	22.435913	8
TACATGG	69730	0.0	22.146847	2
CTATCTC	38060	0.0	22.084715	94
CCGTCTT	13010	0.0	21.830193	90-91
GCCGTCT	13560	0.0	21.676443	90-91
ACATGGG	69805	0.0	21.451332	3
TATGCCG	12955	0.0	20.928625	86-87
ATGCCGT	13650	0.0	20.744337	88-89
>>END_MODULE