FastQCFastQC Report
Fri 27 May 2016
SRR2139939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21354247
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271300.050.1570851
GTATCAA491950.034.4513441
TCAACGC607300.027.1547554
ATCAACG618900.026.6391493
CAACGCA625900.026.3217545
AACGCAG632550.026.0836776
TATCAAC641650.025.9877552
ACGCAGA744150.022.1610167
CGCAGAG757550.021.7743978
GTACATG707950.020.9784871
GTGGTAT121200.020.5297341
TACATGG712750.020.4079882
ACATGGG705450.019.9579123
GCAGAGT873600.018.6581549
GAGTACT480500.018.19515212-13
TGGTATC126350.018.1433372
CATGGGG474350.017.5717644
AGAGTAC795150.017.50943810-11
CAGAGTA857300.017.35206610-11
GTACTTT548900.015.87334214-15
Produced by FastQC (version 0.11.5)