##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21354247
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613932254319245	31.0	31.0	34.0	30.0	34.0
2	31.704882078024106	31.0	31.0	34.0	30.0	34.0
3	31.789338064695045	31.0	31.0	34.0	30.0	34.0
4	35.56199673067376	37.0	35.0	37.0	33.0	37.0
5	35.41321475770136	37.0	35.0	37.0	33.0	37.0
6	35.44469168123793	37.0	35.0	37.0	33.0	37.0
7	35.352214667180725	37.0	35.0	37.0	32.0	37.0
8	35.326845989933524	37.0	35.0	37.0	32.0	37.0
9	36.93323061215879	39.0	37.0	39.0	33.0	39.0
10-11	36.82422906319291	39.0	37.0	39.0	32.0	39.0
12-13	36.780252518386625	39.0	37.0	39.0	32.0	39.0
14-15	37.90091687616051	40.0	37.0	41.0	32.5	41.0
16-17	37.855131791816405	40.0	37.0	41.0	32.0	41.0
18-19	37.881116060894115	40.0	37.0	41.0	32.0	41.0
20-21	37.85126171686509	40.0	37.0	41.0	32.0	41.0
22-23	37.7657858587777	40.0	37.0	41.0	32.0	41.0
24-25	37.65804309026389	39.5	36.5	41.0	32.0	41.0
26-27	37.43853942391807	39.0	36.0	41.0	31.5	41.0
28-29	37.29135614596538	39.0	36.0	41.0	31.0	41.0
30-31	37.11644437466927	39.0	36.0	40.5	31.0	41.0
32-33	36.92297849264944	39.0	35.5	40.0	30.0	41.0
34-35	36.770017431214754	38.5	35.0	40.0	30.0	41.0
36-37	36.59391822219311	38.0	35.0	40.0	30.0	41.0
38-39	36.426810038333485	38.0	35.0	40.0	30.0	41.0
40-41	36.29257428275216	38.0	35.0	40.0	30.0	41.0
42-43	36.09847467593973	38.0	34.5	40.0	29.5	41.0
44-45	36.18488251663587	38.0	34.5	40.0	29.5	41.0
46-47	36.653852189977925	39.0	35.0	40.0	30.0	41.0
48-49	36.752073559331734	39.0	35.0	40.0	30.0	41.0
50-51	36.701478834032315	39.0	35.0	40.0	30.0	41.0
52-53	36.592702627963334	39.0	35.0	40.0	30.0	41.0
54-55	36.437123155265155	38.0	35.0	40.0	30.0	41.0
56-57	36.26017962260847	38.0	34.5	40.0	29.0	41.0
58-59	36.09396707206939	38.0	34.0	40.0	29.0	41.0
60-61	35.90545633101421	38.0	34.0	40.0	28.5	41.0
62-63	35.695673934528855	37.5	34.0	40.0	28.0	41.0
64-65	35.43541524936127	37.0	34.0	40.0	28.0	41.0
66-67	35.17322188743176	36.5	33.0	39.5	28.0	41.0
68-69	34.89249905237671	36.0	33.0	39.0	28.0	41.0
70-71	34.593897455147285	36.0	33.0	39.0	28.0	40.0
72-73	34.291904443737465	35.0	32.0	38.5	27.0	40.0
74-75	33.97611888975189	35.0	32.0	38.0	27.0	40.0
76-77	32.13976119953756	33.5	30.0	35.5	25.5	38.5
78-79	33.26201548259218	34.5	31.5	36.0	26.0	39.0
80-81	33.32506812922256	35.0	32.0	36.0	27.0	39.0
82-83	33.11138492232945	35.0	32.0	36.0	26.5	37.5
84-85	32.88698624409301	34.0	32.0	35.5	26.0	37.0
86-87	32.67840804319214	34.0	31.5	35.0	26.0	37.0
88-89	32.514755537872915	34.0	31.0	35.0	26.0	36.0
90-91	32.381937152402344	34.0	31.0	35.0	26.0	36.0
92-93	32.30778590880241	34.0	31.0	35.0	26.0	36.0
94-95	32.317917427162214	34.0	31.0	35.0	26.5	35.0
96-97	32.410004516855786	34.0	31.0	35.0	27.0	35.0
98-99	32.56317156103755	34.0	31.0	35.0	27.5	35.0
100	31.0073101411776	33.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	48.0
20	281.0
21	1764.0
22	8168.0
23	26568.0
24	66399.0
25	133438.0
26	229194.0
27	343928.0
28	469970.0
29	600155.0
30	732295.0
31	874847.0
32	1045042.0
33	1264758.0
34	1568918.0
35	1999498.0
36	2681731.0
37	3618856.0
38	3706233.0
39	1905829.0
40	76319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07245959082519	21.657724573477115	14.905119342302259	23.364696493395435
2	14.343411827519818	22.247543011569203	41.67784681993585	21.73119834097513
3	17.572964177709668	27.576885646067765	31.60571906870418	23.24443110751838
4	12.324082417890923	19.04630493409578	39.39058586331796	29.239026784695334
5	12.301112748204138	38.84298519165766	36.36350183642626	12.492400223711938
6	28.55349570509323	39.5564919708946	19.040849344863343	12.849162979148831
7	25.179261789834378	33.53243973422801	24.057540974056494	17.230757501881126
8	23.814260460694307	37.667930880447344	21.59598978133015	16.921818877528203
9	25.31894943427413	16.645442941631234	21.54256715303518	36.49304047105946
10-11	23.63625103346156	27.263656034022336	29.658409198563003	19.441683733953102
12-13	24.344562860326377	24.87135471865453	29.710682429181436	21.073399991837658
14-15	21.6589455392123	26.28803019077791	27.064102368679542	24.98892190133024
16-17	21.189044064220447	29.508912440440277	27.6964068326431	21.605636662696174
18-19	21.209722543817918	28.511991453787356	29.411728344083965	20.86655765831076
20-21	22.081818043236986	27.664735792111557	29.219703429900946	21.03374273475051
22-23	21.807651344421746	27.6110471927954	29.154888228822497	21.426413233960357
24-25	21.556385545713898	27.900222723535776	29.162293084381268	21.38109864636906
26-27	21.552646763544058	28.00047982190421	29.072619690223718	21.374253724328014
28-29	21.480718526687635	27.870722718898755	29.139287680693798	21.50927107371981
30-31	22.010509800272438	27.883884636108213	28.873141968777016	21.232463594842336
32-33	21.76774718957776	28.04214528455433	28.88356599078249	21.30654153508542
34-35	21.732776470753027	28.06702207139857	28.93034220576942	21.26985925207898
36-37	21.758166467953867	27.848868007959425	28.953492925635604	21.4394725984511
38-39	21.50922196236011	27.949754264443328	29.06383822621707	21.477185546979488
40-41	21.74168828183521	27.830980379929038	28.969641112062654	21.4576902261731
42-43	21.744400876700055	27.90127573787844	28.703935160592746	21.650388224828752
44-45	21.675323565123243	28.12317857577686	28.472235774218092	21.729262084881807
46-47	21.97098274651548	27.868262714364572	28.181835486494567	21.97891905262538
48-49	21.803704270194395	28.04067105939067	28.231127607529732	21.924497062885205
50-51	21.638990744944234	28.174980769196377	28.17693769986049	22.009090785998904
52-53	21.83040804852044	28.076643765656247	28.138613522206906	21.95433466361641
54-55	21.87443641054349	27.932353011962707	28.093885368912964	22.099325208580844
56-57	21.954677417651595	28.054743730420174	27.962233454916834	22.028345397011392
58-59	21.73314236300536	28.184447501815573	28.113618690361147	21.968791444817917
60-61	21.89205188609322	28.325962763026645	27.91812291930639	21.863862431573743
62-63	21.767231197639	28.4259164401517	27.880431281401552	21.92642108080775
64-65	21.759751589688697	28.400586912501108	27.79850309697764	22.041158400832558
66-67	21.885053085850252	28.421093207383073	27.571418542773753	22.12243516399292
68-69	21.841088063324094	28.316007267907896	27.699360544252293	22.143544124515724
70-71	21.901607897112417	27.934675755144195	27.858032938069076	22.305683409674316
72-73	21.974341897381326	27.94099534146292	27.748285914710756	22.336376846444995
74-75	21.80629638330826	28.181020384230276	27.67690191706378	22.335781315397686
76-77	22.014254399160375	28.086325883153652	27.63382857354021	22.265591144145763
78-79	22.015740218561273	28.114860427137163	27.52811884149552	22.341280512806048
80-81	22.0555836908419	28.067726081046324	27.48847086277729	22.388219365334486
82-83	22.058689493876425	27.99884825467871	27.66585418550262	22.276608065942245
84-85	22.216473513634348	27.960184402417028	27.591253424774	22.232088659174625
86-87	21.953461481705396	28.056672088016082	27.72739399292584	22.26247243735268
88-89	22.08864671454356	27.9869129878947	27.649031788874858	22.27540850868688
90-91	22.168748616644532	27.924655544488715	27.765320661797254	22.1412751770695
92-93	22.251233900065138	27.872171185488188	27.740174595408966	22.136420319037704
94-95	22.00668413424912	27.841312386458643	27.928221622497666	22.223781856794563
96-97	22.00182836986003	27.938401506116726	28.106592694246068	21.953177429777178
98-99	23.008990211796164	29.902850552795808	28.325489664739834	18.7626695706682
100	29.93634396227488	40.504507389844875	29.559148647880246	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3878.0
1	3595.0
2	5582.5
3	8381.0
4	11083.5
5	17988.5
6	24280.0
7	29220.5
8	32842.5
9	32657.5
10	29619.5
11	25947.5
12	27014.5
13	34516.0
14	53138.5
15	83524.0
16	109543.5
17	123190.0
18	128317.0
19	124953.0
20	117117.0
21	109303.0
22	103782.5
23	109169.0
24	131222.5
25	168757.0
26	221617.0
27	281953.5
28	350473.0
29	430273.5
30	501685.5
31	568346.5
32	642266.5
33	713602.0
34	776795.5
35	829055.0
36	874781.5
37	903473.0
38	902557.0
39	882809.0
40	866318.0
41	847418.0
42	820779.5
43	802490.5
44	791478.0
45	778172.5
46	765827.5
47	765761.5
48	777473.0
49	773758.5
50	753788.5
51	737221.0
52	713612.0
53	685350.5
54	654737.0
55	615921.0
56	571058.0
57	524575.5
58	476385.0
59	419970.5
60	358887.0
61	298540.5
62	241341.5
63	191081.5
64	147518.5
65	113327.5
66	87651.0
67	69193.5
68	54580.0
69	41189.0
70	30803.0
71	23331.5
72	17848.0
73	13501.0
74	9937.5
75	7155.0
76	5210.0
77	3760.5
78	2594.5
79	1814.5
80	1327.0
81	981.5
82	726.5
83	522.5
84	374.0
85	284.5
86	224.5
87	180.5
88	125.5
89	76.0
90	48.5
91	33.0
92	27.0
93	21.5
94	15.0
95	9.5
96	6.5
97	6.0
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.12653220691883915
3	4.682909212392271E-6
4	0.0
5	0.0
6	0.0
7	1.4048727637176811E-5
8	0.0
9	0.0
10-11	1.9668218692047538E-4
12-13	3.8634001002236227E-4
14-15	8.265334759872357E-4
16-17	2.3882836983200577E-4
18-19	0.0010606789366068492
20-21	7.259009660336888E-5
22-23	0.0018712590627254467
24-25	1.3822390759595898E-4
26-27	0.00545478144411342
28-29	0.0027986390028512573
30-31	0.004659473877022739
32-33	0.01731946607119189
34-35	0.0038896906446693777
36-37	0.0021411704103762447
38-39	0.0011480822077673586
40-41	6.260322734381347E-4
42-43	0.0013124226192086517
44-45	4.687514956873382E-5
46-47	2.2732206933328113E-4
48-49	1.6424740348860474E-4
50-51	4.700699080814061E-4
52-53	1.2898003696000348E-5
54-55	0.0
56-57	0.0
58-59	1.4394995531820046E-4
60-61	0.0
62-63	1.3677750021063734E-5
64-65	5.8223212213987825E-5
66-67	0.0
68-69	2.629275398278779E-4
70-71	9.577440704105579E-5
72-73	9.99125838573095E-4
74-75	1.2268771804375451E-4
76-77	0.002318668640523247
78-79	0.002799013920048621
80-81	0.002897149528164829
82-83	8.306155963732129E-5
84-85	0.0
86-87	0.0018312945858553786
88-89	4.866292858776059E-4
90-91	6.501728422250464E-4
92-93	9.579615939714554E-5
94-95	4.0188381210178105E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	918.0
20-21	2920.0
22-23	5444.0
24-25	11653.0
26-27	13669.0
28-29	26619.0
30-31	59678.0
32-33	76759.0
34-35	105804.0
36-37	149587.0
38-39	170362.0
40-41	184460.0
42-43	212593.0
44-45	258605.0
46-47	232191.0
48-49	219470.0
50-51	195789.0
52-53	183260.0
54-55	201444.0
56-57	228284.0
58-59	235039.0
60-61	242772.0
62-63	241532.0
64-65	253851.0
66-67	278947.0
68-69	267885.0
70-71	265712.0
72-73	256020.0
74-75	260515.0
76-77	244440.0
78-79	268934.0
80-81	279999.0
82-83	271690.0
84-85	276489.0
86-87	295573.0
88-89	328301.0
90-91	359175.0
92-93	393585.0
94-95	464607.0
96-97	899918.0
98-99	4199270.0
100-101	8230484.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.12941959960138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03843073334298	74.0171306892211
2	7.452513798575378	12.390462932671833
3	1.864753071998439	4.650475217154117
4	0.7270858601634368	2.417689022178537
5	0.3289570707632421	1.3673005182866655
6	0.17837210692565544	0.8896781832892664
7	0.1077838159711255	0.6272004244736936
8	0.07345309514227656	0.48848905335773996
9	0.048644312314499775	0.36393961045734974
>10	0.17436880907664676	2.2478820383297218
>50	0.003991361930456032	0.22293113465625689
>100	0.0015343943065512509	0.21181286395304627
>500	4.473167796572197E-5	0.025175466310126727
>1k	6.683781124796835E-5	0.0798328456605901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.68290921239227E-6	9.36581842478454E-6
2	0.0	1.873163684956908E-5	0.0	4.68290921239227E-6	9.36581842478454E-6
3	0.0	2.341454606196135E-5	0.0	4.68290921239227E-6	9.36581842478454E-6
4	0.0	5.6194910548707246E-5	0.0	1.4048727637176811E-5	9.36581842478454E-6
5	0.0	6.087781976109951E-5	0.0	1.4048727637176811E-5	9.36581842478454E-6
6	0.0	6.087781976109951E-5	0.0	1.4048727637176811E-5	1.4048727637176811E-5
7	0.0	7.024363818588406E-5	0.0	1.873163684956908E-5	1.873163684956908E-5
8	0.0	8.897527503545313E-5	0.0	1.873163684956908E-5	2.341454606196135E-5
9	0.0	1.73267640858514E-4	0.0	2.8097455274353623E-5	3.746327369913816E-5
10-11	4.68290921239227E-6	1.73267640858514E-4	0.0	3.746327369913816E-5	3.746327369913816E-5
12-13	4.68290921239227E-6	1.7560909546471013E-4	0.0	3.9804728305334295E-5	3.746327369913816E-5
14-15	4.68290921239227E-6	2.271210968010251E-4	0.0	4.4487637517726567E-5	4.4487637517726567E-5
16-17	4.68290921239227E-6	2.411698244382019E-4	0.0	4.68290921239227E-5	6.087781976109951E-5
18-19	4.68290921239227E-6	2.6224291589396716E-4	0.0	6.321927436729565E-5	7.258509279208018E-5
20-21	4.68290921239227E-6	2.9034037116832073E-4	0.0	1.1004836649121834E-4	7.960945661066859E-5
22-23	4.68290921239227E-6	3.2077928104887047E-4	0.0	4.1912037450910817E-4	1.0770691188502222E-4
24-25	7.024363818588405E-6	3.605840093542048E-4	0.0	0.001456384765053996	1.5687745861514104E-4
26-27	9.36581842478454E-6	3.8165710080997005E-4	0.0	0.004062423741750294	1.6390182243372945E-4
28-29	9.36581842478454E-6	3.8399855541616616E-4	0.0	0.014826090566433928	1.7560909546471013E-4
30-31	9.36581842478454E-6	4.5424219360205024E-4	0.0	0.018867441216728457	1.7795055007090627E-4
32-33	9.36581842478454E-6	4.589251028144425E-4	0.0	0.027579993806384276	1.873163684956908E-4
34-35	9.36581842478454E-6	5.315101956065227E-4	0.0	0.037479663881381534	1.9668218692047535E-4
36-37	9.36581842478454E-6	6.087781976109952E-4	0.0	0.053586530117404746	1.9668218692047535E-4
38-39	9.36581842478454E-6	6.368756528853487E-4	0.0	0.10080430370595601	2.0370655073906376E-4
40-41	9.36581842478454E-6	6.883876542216638E-4	0.0	0.1486261725829059	2.2243818758863285E-4
42-43	9.36581842478454E-6	7.024363818588405E-4	0.0	0.17970898248015957	2.2946255140722125E-4
44-45	9.36581842478454E-6	7.562898378013516E-4	0.0	0.21996795297909594	2.388283698320058E-4
46-47	9.36581842478454E-6	8.054603845314705E-4	0.0	0.26102535949874517	2.59901461287771E-4
48-49	9.36581842478454E-6	8.054603845314705E-4	0.0	0.299141899033012	2.8097455274353624E-4
50-51	9.36581842478454E-6	8.897527503545314E-4	0.0	0.3375066327555357	2.8097455274353624E-4
52-53	9.36581842478454E-6	8.991185687793159E-4	0.0	0.432536909402612	3.043890988054976E-4
54-55	9.36581842478454E-6	9.01460023385512E-4	0.0	0.5487362771443076	3.324865540798512E-4
56-57	9.36581842478454E-6	9.17850205628885E-4	0.0	0.66181682735055	3.3716946329224345E-4
58-59	9.36581842478454E-6	9.225331148412772E-4	0.0	0.7253849784541688	3.3951091789843956E-4
60-61	9.36581842478454E-6	9.553134793280232E-4	0.0	0.76250171687159	3.418523725046357E-4
62-63	9.36581842478454E-6	9.810694799961806E-4	0.0	0.7906740986933418	3.5121819092942026E-4
64-65	9.36581842478454E-6	0.00107238620963783	0.0	0.8028379553725308	3.5121819092942026E-4
66-67	9.36581842478454E-6	0.0010794105734564183	0.0	0.8137702069288606	3.5590110014181253E-4
68-69	9.36581842478454E-6	0.001107508028730772	0.0	0.8240328024678182	3.6292546396040097E-4
70-71	9.36581842478454E-6	0.0011356054840051255	0.0	0.8311648731982917	3.722912823851855E-4
72-73	9.36581842478454E-6	0.0011473127570361063	0.0	0.8364190973345957	4.120960106905198E-4
74-75	9.36581842478454E-6	0.001177751666916656	0.0	0.8403550825276115	4.120960106905198E-4
76-77	9.36581842478454E-6	0.001231605122859167	0.0	0.8441435560804369	4.144374652967159E-4
78-79	9.36581842478454E-6	0.0012503367597087361	0.0	0.8468245216045314	4.191203745091082E-4
80-81	1.4048727637176811E-5	0.0012573611235273245	0.0	0.8477002256272488	4.3551055675248115E-4
82-83	1.4048727637176811E-5	0.001262044032739717	0.0	0.8484143692821386	4.6829092123922705E-4
84-85	1.4048727637176811E-5	0.001264385487345913	0.0	0.8486508561973645	4.7531528505781543E-4
86-87	1.4048727637176811E-5	0.001283117124195482	0.0	0.8488077336559796	4.7765673966401154E-4
88	1.4048727637176811E-5	0.0013393120347441894	0.0	0.8488334896566477	4.823396488764038E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27130	0.0	50.157085	1
GTATCAA	49195	0.0	34.451344	1
TCAACGC	60730	0.0	27.154755	4
ATCAACG	61890	0.0	26.639149	3
CAACGCA	62590	0.0	26.321754	5
AACGCAG	63255	0.0	26.083677	6
TATCAAC	64165	0.0	25.987755	2
ACGCAGA	74415	0.0	22.161016	7
CGCAGAG	75755	0.0	21.774397	8
GTACATG	70795	0.0	20.978487	1
GTGGTAT	12120	0.0	20.529734	1
TACATGG	71275	0.0	20.407988	2
ACATGGG	70545	0.0	19.957912	3
GCAGAGT	87360	0.0	18.658154	9
GAGTACT	48050	0.0	18.195152	12-13
TGGTATC	12635	0.0	18.143337	2
CATGGGG	47435	0.0	17.571764	4
AGAGTAC	79515	0.0	17.509438	10-11
CAGAGTA	85730	0.0	17.352066	10-11
GTACTTT	54890	0.0	15.873342	14-15
>>END_MODULE