FastQCFastQC Report
Fri 27 May 2016
SRR2139938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21495104
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256950.047.9872931
GTATCAA474450.033.6598471
ATCAACG557650.027.9227753
TCAACGC575200.027.0639024
CAACGCA592500.026.2601225
AACGCAG594650.026.1923146
ACGCAGA609250.025.5451077
TATCAAC618350.025.344622
CGCAGAG709900.021.9120948
ACATGGG598700.020.4134863
GTACATG631400.020.4011691
TACATGG638450.019.8426572
GCAGAGT825600.018.6568079
GTGGTAT126050.017.7711321
GAGTACT490650.017.50597212-13
AGAGTAC761500.017.39234410-11
CATGGGG407700.017.1409174
CAGAGTA826750.016.6925310-11
CGTCGAA10752.9394869E-916.60642294
TGGTATC132000.015.3509382
Produced by FastQC (version 0.11.5)