FastQCFastQC Report
Fri 27 May 2016
SRR2139937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24298458
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273500.048.113071
GTATCAA492000.032.963221
ATCAACG577350.027.2938613
TCAACGC592150.026.5841374
CAACGCA607750.025.8816365
AACGCAG608400.025.8473346
ACGCAGA624900.025.1779067
TATCAAC645850.024.6484362
CGCAGAG724950.021.6750778
GTACATG625950.019.8674661
ACATGGG595450.019.5878943
TACATGG634350.019.0386892
GCAGAGT855000.018.187699
AGAGTAC779800.017.61193310-11
GAGTACT531600.017.33061212-13
CATGGGG411200.016.7114374
GTGGTAT144200.016.6818771
CAGAGTA850650.016.66284210-11
TGGTATC140250.015.5746632
AGTACTT615250.014.91466712-13
Produced by FastQC (version 0.11.5)