##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24298458
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.814984555810085	31.0	31.0	34.0	30.0	34.0
2	31.923195373138494	31.0	31.0	34.0	30.0	34.0
3	32.67061716426615	34.0	31.0	34.0	30.0	37.0
4	35.56284793874574	37.0	35.0	37.0	33.0	37.0
5	35.45191423258217	37.0	35.0	37.0	33.0	37.0
6	35.459796337693525	37.0	35.0	37.0	33.0	37.0
7	35.44484390737881	37.0	35.0	37.0	33.0	37.0
8	35.710484673554184	37.0	35.0	37.0	32.0	39.0
9	36.94601352892435	39.0	37.0	39.0	33.0	39.0
10-11	36.86434709149033	39.0	37.0	39.0	32.0	39.0
12-13	36.96204466966587	39.0	37.0	39.0	32.0	39.5
14-15	37.959973200768545	40.0	37.0	41.0	33.0	41.0
16-17	37.93310511720538	40.0	37.0	41.0	33.0	41.0
18-19	37.965357822294735	40.0	37.0	41.0	33.0	41.0
20-21	37.940160675757255	40.0	37.0	41.0	32.0	41.0
22-23	37.844969135550855	40.0	37.0	41.0	32.0	41.0
24-25	37.73838387888861	40.0	37.0	41.0	32.0	41.0
26-27	37.5648020333517	39.0	36.5	41.0	32.0	41.0
28-29	37.44492221287277	39.0	36.0	41.0	32.0	41.0
30-31	37.27324648834421	39.0	36.0	41.0	31.0	41.0
32-33	37.09202181101644	39.0	36.0	40.5	31.0	41.0
34-35	36.971711698151246	39.0	36.0	40.0	30.0	41.0
36-37	36.829622656147535	39.0	35.0	40.0	30.0	41.0
38-39	36.69170391358648	38.0	35.0	40.0	30.0	41.0
40-41	36.574487776324084	38.0	35.0	40.0	30.0	41.0
42-43	36.396533094094075	38.0	35.0	40.0	30.0	41.0
44-45	36.27803944030671	38.0	35.0	40.0	30.0	41.0
46-47	36.451117556076355	38.0	35.0	40.0	30.0	41.0
48-49	36.82097917702973	39.0	35.0	40.5	30.0	41.0
50-51	36.900657793539324	39.0	35.0	41.0	30.0	41.0
52-53	36.86780582362752	39.0	35.0	41.0	30.0	41.0
54-55	36.76918300546609	39.0	35.0	40.5	30.0	41.0
56-57	36.64068461182731	39.0	35.0	40.0	30.0	41.0
58-59	36.46071706751377	38.0	35.0	40.0	29.5	41.0
60-61	36.30829559825341	38.0	34.5	40.0	29.0	41.0
62-63	36.11468563296568	38.0	34.0	40.0	29.0	41.0
64-65	35.89037530175142	38.0	34.0	40.0	29.0	41.0
66-67	35.65129917170401	37.0	34.0	40.0	28.0	41.0
68-69	35.39432066135343	37.0	34.0	39.5	28.0	41.0
70-71	35.12665530108425	36.0	33.5	39.0	28.0	41.0
72-73	34.81642598724092	36.0	33.0	39.0	28.0	40.5
74-75	34.28302360841779	35.5	32.5	38.5	27.5	40.0
76-77	32.92673593420506	34.0	30.5	36.5	26.0	39.0
78-79	33.81921155347263	35.0	32.0	37.0	27.5	39.0
80-81	33.78972014666856	35.0	32.0	37.0	28.0	39.0
82-83	33.54167865204343	35.0	32.0	36.0	28.0	38.5
84-85	33.305668197296825	35.0	32.0	36.0	27.5	37.0
86-87	33.081314174791714	35.0	32.0	35.5	27.0	37.0
88-89	32.91824809077917	34.0	32.0	35.0	27.0	36.5
90-91	32.77783501647076	34.0	32.0	35.0	27.0	36.0
92-93	32.689145233285444	34.0	32.0	35.0	27.0	36.0
94-95	32.666153290310135	34.0	32.0	35.0	27.0	36.0
96-97	32.73319470718292	34.0	32.0	35.0	28.5	35.0
98-99	32.70774757829918	34.0	32.0	35.0	28.0	35.0
100	31.473415270787765	33.0	30.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	6.0
19	56.0
20	279.0
21	1797.0
22	8268.0
23	26994.0
24	67150.0
25	135072.0
26	231446.0
27	350940.0
28	483934.0
29	621826.0
30	768756.0
31	925921.0
32	1109237.0
33	1348058.0
34	1673205.0
35	2137267.0
36	2890896.0
37	4059285.0
38	4666592.0
39	2640902.0
40	150569.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.507378000596525	4.116785803017151E-5	27.3633201383487	29.129260693196745
2	14.448236655972293	26.427115592806487	38.56340053665371	20.56124721456751
3	15.7090384074277	26.529176695255185	34.890047106294965	22.871737791022152
4	12.52420633231974	25.145414960346763	38.78615187489346	23.54422683244004
5	15.927232913298447	38.154371771245735	33.36978832154699	12.548606993908832
6	25.449611882762763	39.439257398113796	21.726375464911207	13.384755254212234
7	23.71320218864935	34.25754706389575	24.63131813807429	17.39793260938061
8	22.584457828558506	33.34377020961577	23.779204425235545	20.29256753659018
9	22.50894830666579	21.284121309456257	25.701962413078828	30.50496797079913
10-11	23.312047397613636	27.085563543517416	30.50840900743478	19.09398005143417
12-13	23.394690964682948	25.447789466523258	30.176567475337773	20.980952093456022
14-15	21.001144195837995	28.193949302107214	27.838471582853902	22.966434919200893
16-17	20.2225532855689	30.172464669717876	28.954947408311487	20.65003463640174
18-19	20.346280340535525	29.334207299120596	30.388026240703415	19.931486119640468
20-21	20.986143826487563	28.78256942028991	30.213433696020758	20.01785305720177
22-23	20.934520945829096	28.407803225486557	30.00567136519603	20.652004463488318
24-25	20.652186356183385	28.72526321242405	30.09275345656372	20.529796974828837
26-27	20.60047473193703	28.87566391362139	29.95477225749678	20.569089096944797
28-29	20.731556984149417	28.677594590373396	29.97872478806344	20.612123637413738
30-31	20.930675092811267	28.673656194771247	29.86158795503307	20.534080757384416
32-33	20.714009842588148	29.04225512904764	29.727898894411524	20.515836133952682
34-35	20.881478092882556	28.809148367902925	29.673680490598514	20.635693048616
36-37	20.816830332519334	28.806646006324403	29.767264816560555	20.6092588445957
38-39	20.67627043982167	29.059962295454106	29.71973066734852	20.544036597375705
40-41	20.957770928788534	28.792507359413268	29.515376985446046	20.73434472635215
42-43	20.899668515847694	28.867978173025005	29.417458045090715	20.81489526603658
44-45	20.959628477115164	29.186197955402886	29.06519165725579	20.78898191022616
46-47	21.104660922155862	28.868605320690527	28.93866764214023	21.088066115013383
48-49	20.96617945916319	29.089030668536363	28.95606590692311	20.988723965377332
50-51	20.912196859330212	29.30977540260052	28.802693151048047	20.97533458702122
52-53	21.165153607753584	28.985621701726245	28.711450921650155	21.13777376887002
54-55	21.095119729908202	28.89810062245403	28.866624424570382	21.14015522306739
56-57	21.105098868209318	29.216009313304863	28.62850991625463	21.05038190223119
58-59	21.21701269560492	28.98876034917994	28.679217976205006	21.115008979010135
60-61	21.13213817816822	29.082168475391136	28.745267352122607	21.040425994318042
62-63	21.082501554304965	29.261287017468586	28.622763035724898	21.03344839250155
64-65	21.214635796344215	28.985334564699887	28.5731279346867	21.226901704269206
66-67	21.13795270969657	29.089480205469858	28.52100161937806	21.251565465455517
68-69	21.169057556043857	29.15802438490795	28.557927980017023	21.114990079031177
70-71	21.279462860529506	28.731821731598682	28.638129887205405	21.350585520666403
72-73	21.22868582170113	28.88656782901185	28.516014922145168	21.368731427141853
74-75	21.010023830091495	29.12709872127937	28.58759587056682	21.275281578062316
76-77	21.279179212794208	28.850146000433437	28.47085044870838	21.39982433806398
78-79	21.128360605300514	28.974310504430484	28.545043644826958	21.352285245442044
80-81	21.242625915643544	28.997594357154235	28.430800002269656	21.328979724932566
82-83	21.3174234596556	28.776150501915716	28.562219762902096	21.344206275526588
84-85	21.321134317054778	28.735173437382898	28.64412543601115	21.29956680955117
86-87	21.199977567966364	28.885096467990873	28.583707145240577	21.33121881880219
88-89	21.28923625910066	28.683552873762146	28.57156646398129	21.4556444031559
90-91	21.35917035312946	28.668159450596796	28.68451732552535	21.28815287074839
92-93	21.270430755104435	28.707008274902318	28.690302036044752	21.3322589339485
94-95	21.278083032500007	28.51942381218185	28.77298789277585	21.429505262542296
96-97	21.218894372199067	28.896148580641462	28.906263079804024	20.978693967355447
98-99	22.18233771953273	30.98723614966839	28.466188627981452	18.364237502817428
100	21.781469453162313	30.782870379103866	22.981142603537958	24.454517564195868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4833.0
1	4317.5
2	6249.5
3	9117.5
4	11847.0
5	19709.5
6	27091.5
7	32298.0
8	35868.5
9	35347.0
10	32013.5
11	28299.5
12	29538.5
13	36888.5
14	56889.5
15	88828.5
16	117795.5
17	136497.5
18	144894.0
19	145056.0
20	140859.5
21	135887.0
22	136455.5
23	151184.0
24	184295.5
25	237839.5
26	313667.5
27	404003.0
28	504827.0
29	617395.0
30	719744.0
31	809089.5
32	897864.5
33	975680.0
34	1041966.0
35	1087081.5
36	1122799.5
37	1147311.5
38	1133832.0
39	1099613.5
40	1068258.5
41	1033307.5
42	987375.0
43	946915.5
44	909731.0
45	875100.0
46	840229.0
47	804099.5
48	774859.5
49	745290.0
50	718991.0
51	692668.5
52	661165.0
53	628507.5
54	594587.5
55	557658.5
56	516067.0
57	470267.0
58	423071.0
59	373892.0
60	321033.0
61	267956.0
62	217615.5
63	172373.0
64	133360.0
65	102363.5
66	78502.5
67	60916.5
68	47933.0
69	36559.5
70	27613.5
71	21139.0
72	15986.0
73	11957.5
74	8943.0
75	6718.5
76	5065.0
77	3698.5
78	2568.0
79	1788.0
80	1260.0
81	901.5
82	685.5
83	508.0
84	374.5
85	272.0
86	206.0
87	160.0
88	112.5
89	83.0
90	62.5
91	46.0
92	35.5
93	21.0
94	9.5
95	5.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03153698065943114
2	0.1078051948810908
3	2.8808412451522642E-5
4	2.6339119955677846E-4
5	0.0
6	4.1154874930746635E-6
7	8.230974986149327E-6
8	0.0
9	2.4692924958447983E-5
10-11	8.025200611495594E-5
12-13	5.5559081156507954E-5
14-15	6.996328738226928E-4
16-17	0.0013251869727700415
18-19	9.877169983379193E-5
20-21	5.432811738133042E-4
22-23	0.0020438232337832247
24-25	6.259128753984532E-4
26-27	0.004831019985507352
28-29	0.004167352719599744
30-31	0.00938551486779801
32-33	0.008323129417077113
34-35	0.0038794938467668614
36-37	0.006967909552179069
38-39	2.4261483091803237E-4
40-41	4.5738511017875456E-4
42-43	0.0016342754419426547
44-45	8.685960530213614E-6
46-47	2.4187469778031374E-5
48-49	1.0454767481856028E-4
50-51	3.371900153180928E-5
52-53	5.495011279078317E-4
54-55	0.002083530815569914
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	1.234545374591807E-5
68-69	2.6545332792075665E-4
70-71	6.377875611961612E-4
72-73	4.434250823655645E-4
74-75	6.434444260188001E-4
76-77	0.0018981939388198552
78-79	0.006333044369648558
80-81	7.86698038101184E-4
82-83	4.5446378793504035E-4
84-85	0.0
86-87	0.0
88-89	0.0
90-91	2.70726065649988E-5
92-93	3.41948399801633E-4
94-95	8.549017660877315E-5
96-97	9.227947709702474E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	938.0
20-21	3493.0
22-23	6520.0
24-25	13022.0
26-27	19261.0
28-29	36175.0
30-31	72131.0
32-33	93540.0
34-35	131839.0
36-37	172717.0
38-39	195099.0
40-41	212073.0
42-43	247093.0
44-45	286895.0
46-47	268456.0
48-49	239961.0
50-51	224006.0
52-53	225447.0
54-55	244963.0
56-57	258903.0
58-59	258018.0
60-61	255216.0
62-63	248040.0
64-65	263758.0
66-67	282890.0
68-69	288775.0
70-71	285159.0
72-73	278360.0
74-75	284191.0
76-77	281044.0
78-79	302138.0
80-81	310211.0
82-83	299283.0
84-85	306406.0
86-87	324731.0
88-89	360104.0
90-91	384811.0
92-93	422655.0
94-95	528994.0
96-97	1282447.0
98-99	4026785.0
100-101	1.007191E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.68388010770528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.65451065657714	73.30298930135704
2	7.7148660891936265	12.757901255317739
3	1.9360973337148029	4.802521194531678
4	0.7093641495947707	2.346119211911933
5	0.3497088430441721	1.4457642025434325
6	0.20503211929138515	1.017171070183057
7	0.11446775571386943	0.6625246732750572
8	0.07503771458156391	0.4963527516814591
9	0.05053869129297872	0.37608615825020836
>10	0.18540006305361065	2.3905457708707116
>50	0.003945481081117886	0.214238313956983
>100	9.525307763715387E-4	0.12165481208491619
>500	3.9324057662312186E-5	0.02665429045222542
>1k	3.924802692774182E-5	0.03947699358352258
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.1154874930746635E-6	0.0
2	0.0	2.0577437465373317E-5	0.0	4.1154874930746635E-6	0.0
3	0.0	2.0577437465373317E-5	0.0	4.1154874930746635E-6	0.0
4	0.0	2.0577437465373317E-5	0.0	8.230974986149327E-6	0.0
5	0.0	2.469292495844798E-5	0.0	8.230974986149327E-6	0.0
6	3.7039387437671974E-5	2.8808412451522645E-5	0.0	1.234646247922399E-5	4.1154874930746635E-6
7	3.7039387437671974E-5	3.292389994459731E-5	0.0	1.6461949972298654E-5	4.1154874930746635E-6
8	3.7039387437671974E-5	3.292389994459731E-5	0.0	1.6461949972298654E-5	4.1154874930746635E-6
9	3.7039387437671974E-5	6.996328738226928E-5	0.0	2.8808412451522645E-5	1.234646247922399E-5
10-11	3.7039387437671974E-5	6.996328738226928E-5	0.0	5.7616824903045284E-5	1.234646247922399E-5
12-13	3.7039387437671974E-5	7.407877487534395E-5	0.0	8.230974986149327E-5	2.0577437465373317E-5
14-15	3.7039387437671974E-5	1.172913935526279E-4	0.0	9.054072484764258E-5	3.7039387437671974E-5
16-17	4.52703624238213E-5	1.2758011228531456E-4	0.0	1.0700267481994125E-4	4.115487493074663E-5
18-19	4.52703624238213E-5	1.2758011228531456E-4	0.0	1.5433078099029988E-4	5.3501337409970625E-5
20-21	4.52703624238213E-5	1.3786883101800124E-4	0.0	1.9754339966758386E-4	5.761682490304529E-5
22-23	4.52703624238213E-5	1.481575497506879E-4	0.0	2.3046729961218114E-4	6.173231239611996E-5
24-25	4.732810617035863E-5	1.6050401222991186E-4	0.0	2.3869827459833048E-4	6.584779988919462E-5
26-27	4.938584991689596E-5	1.6461949972298653E-4	0.0	3.3541223068558505E-4	6.584779988919462E-5
28-29	5.3501337409970625E-5	1.6461949972298653E-4	0.0	7.346145175138273E-4	6.996328738226928E-5
30-31	5.761682490304529E-5	1.7696596220221052E-4	0.0	0.002207959040034557	7.202103112880661E-5
32-33	5.967456864958262E-5	1.7696596220221052E-4	0.0	0.004934469504196522	7.819426236841861E-5
34-35	7.407877487534395E-5	1.9137016842797185E-4	0.0	0.008449095823282283	7.819426236841861E-5
36-37	7.407877487534395E-5	2.0577437465373318E-4	0.0	0.014239586726038336	8.025200611495593E-5
38-39	7.407877487534395E-5	2.0988986214680783E-4	0.0	0.021110393095726487	8.642523735456793E-5
40-41	7.407877487534395E-5	2.3046729961218116E-4	0.0	0.02919938376336474	8.642523735456793E-5
42-43	7.819426236841861E-5	2.551602245706291E-4	0.0	0.03759292050549051	9.054072484764258E-5
44-45	7.819426236841861E-5	2.819108932756144E-4	0.0	0.04674988017758164	9.465621234071726E-5
46-47	7.819426236841861E-5	2.839686370221518E-4	0.0	0.05849753922656327	9.877169983379192E-5
48-49	8.43674936080306E-5	2.839686370221518E-4	0.0	0.06776973254846047	1.0288718732686659E-4
50-51	9.05407248476426E-5	3.045460744875251E-4	0.0	0.07869840958632025	1.0494493107340391E-4
52-53	9.05407248476426E-5	3.0866156198059976E-4	0.0	0.08756316964640308	1.1523364980609058E-4
54-55	9.05407248476426E-5	3.1071930572713707E-4	0.0	0.0982469751784249	1.234646247922399E-4
56-57	9.05407248476426E-5	3.210080244598237E-4	0.0	0.10823526332411712	1.3786883101800124E-4
58-59	9.05407248476426E-5	3.3335448693904774E-4	0.0	0.11846636523190072	1.4404206225761322E-4
60-61	9.05407248476426E-5	3.457009494182717E-4	0.0	0.12785173445985748	1.502152934972252E-4
62-63	1.0288718732686659E-4	3.457009494182717E-4	0.0	0.1353707301097049	1.5638852473683721E-4
64-65	1.0288718732686659E-4	3.703938743767197E-4	0.0	0.14257694871007864	1.5638852473683721E-4
66-67	1.0288718732686659E-4	3.7862484936286903E-4	0.0	0.1497399546917751	1.5638852473683721E-4
68-69	1.0288718732686659E-4	3.868558243490184E-4	0.0	0.15658606813650477	1.5638852473683721E-4
70-71	1.0700267481994125E-4	3.9714454308170503E-4	0.0	0.16249590817656	1.625617559764492E-4
72-73	1.1111816231301591E-4	3.9920228682824234E-4	0.0	0.16735629890588116	1.7285047470913585E-4
74-75	1.1111816231301591E-4	4.0126003057477965E-4	0.0	0.1715294032238589	1.7285047470913585E-4
76-77	1.1111816231301591E-4	4.09491005560929E-4	0.0	0.17523334196762608	1.7285047470913585E-4
78-79	1.1111816231301591E-4	4.1566423680054103E-4	0.0	0.17746393618887257	1.7285047470913585E-4
80-81	1.1111816231301591E-4	4.1772198054707834E-4	0.0	0.17824176332506367	1.7902370594874786E-4
82-83	1.1111816231301591E-4	4.1977972429361565E-4	0.0	0.1788776061427437	1.8519693718835984E-4
84-85	1.1111816231301591E-4	4.2183746804015296E-4	0.0	0.1791142466735955	1.8519693718835984E-4
86-87	1.1111816231301591E-4	4.3624167426591434E-4	0.0	0.17921096062968275	1.8725468093489718E-4
88	1.1111816231301591E-4	4.609345992243623E-4	0.0	0.17922124934841543	1.8931242468143452E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27350	0.0	48.11307	1
GTATCAA	49200	0.0	32.96322	1
ATCAACG	57735	0.0	27.293861	3
TCAACGC	59215	0.0	26.584137	4
CAACGCA	60775	0.0	25.881636	5
AACGCAG	60840	0.0	25.847334	6
ACGCAGA	62490	0.0	25.177906	7
TATCAAC	64585	0.0	24.648436	2
CGCAGAG	72495	0.0	21.675077	8
GTACATG	62595	0.0	19.867466	1
ACATGGG	59545	0.0	19.587894	3
TACATGG	63435	0.0	19.038689	2
GCAGAGT	85500	0.0	18.18769	9
AGAGTAC	77980	0.0	17.611933	10-11
GAGTACT	53160	0.0	17.330612	12-13
CATGGGG	41120	0.0	16.711437	4
GTGGTAT	14420	0.0	16.681877	1
CAGAGTA	85065	0.0	16.662842	10-11
TGGTATC	14025	0.0	15.574663	2
AGTACTT	61525	0.0	14.914667	12-13
>>END_MODULE